LEM3 family/CDC50 family Ros3 protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Species:

Leishmania braziliensis

UniProt:

A4HK31_LEIBR

TriTrypDb:

LbrM.32.0580 , LBRM2903_320010900

Length:

363

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 21
NetGPI	no	yes: 0, no: 21

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	22
GO:0110165	cellular anatomical entity	1	22
GO:0005783	endoplasmic reticulum	5	1
GO:0005794	Golgi apparatus	5	1
GO:0005886	plasma membrane	3	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HK31

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HK31

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	22
GO:0051179	localization	1	22

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	216	218	PF00675	0.539
CLV_PCSK_KEX2_1	216	218	PF00082	0.407
CLV_PCSK_SKI1_1	124	128	PF00082	0.556
CLV_PCSK_SKI1_1	207	211	PF00082	0.578
CLV_PCSK_SKI1_1	97	101	PF00082	0.545
DOC_CYCLIN_yCln2_LP_2	106	112	PF00134	0.294
DOC_MAPK_FxFP_2	149	152	PF00069	0.351
DOC_MAPK_gen_1	207	215	PF00069	0.321
DOC_MAPK_gen_1	284	293	PF00069	0.326
DOC_MAPK_MEF2A_6	25	33	PF00069	0.490
DOC_MAPK_MEF2A_6	284	293	PF00069	0.333
DOC_PP1_RVXF_1	95	101	PF00149	0.346
DOC_PP2B_LxvP_1	106	109	PF13499	0.325
DOC_PP4_FxxP_1	149	152	PF00568	0.311
DOC_PP4_FxxP_1	255	258	PF00568	0.207
DOC_PP4_FxxP_1	356	359	PF00568	0.407
DOC_USP7_MATH_1	277	281	PF00917	0.375
DOC_USP7_MATH_1	320	324	PF00917	0.378
DOC_USP7_UBL2_3	7	11	PF12436	0.726
DOC_WW_Pin1_4	104	109	PF00397	0.326
DOC_WW_Pin1_4	114	119	PF00397	0.364
DOC_WW_Pin1_4	2	7	PF00397	0.685
DOC_WW_Pin1_4	24	29	PF00397	0.584
DOC_WW_Pin1_4	306	311	PF00397	0.395
LIG_14-3-3_CanoR_1	55	63	PF00244	0.330
LIG_14-3-3_CanoR_1	97	103	PF00244	0.356
LIG_14-3-3_CterR_2	360	363	PF00244	0.720
LIG_Actin_WH2_2	123	138	PF00022	0.349
LIG_AP2alpha_2	246	248	PF02296	0.349
LIG_BRCT_BRCA1_1	116	120	PF00533	0.290
LIG_BRCT_BRCA1_1	31	35	PF00533	0.447
LIG_eIF4E_1	114	120	PF01652	0.355
LIG_FHA_1	105	111	PF00498	0.348
LIG_FHA_1	125	131	PF00498	0.151
LIG_FHA_1	137	143	PF00498	0.328
LIG_FHA_1	277	283	PF00498	0.347
LIG_FHA_1	40	46	PF00498	0.382
LIG_FHA_1	54	60	PF00498	0.391
LIG_FHA_2	126	132	PF00498	0.389
LIG_FHA_2	55	61	PF00498	0.387
LIG_LIR_Gen_1	297	307	PF02991	0.323
LIG_LIR_Gen_1	313	320	PF02991	0.355
LIG_LIR_Gen_1	60	71	PF02991	0.345
LIG_LIR_Nem_3	117	123	PF02991	0.352
LIG_LIR_Nem_3	285	289	PF02991	0.312
LIG_LIR_Nem_3	297	303	PF02991	0.293
LIG_LIR_Nem_3	313	319	PF02991	0.243
LIG_LIR_Nem_3	32	38	PF02991	0.420
LIG_LIR_Nem_3	60	66	PF02991	0.310
LIG_NRBOX	331	337	PF00104	0.481
LIG_PTB_Apo_2	119	126	PF02174	0.387
LIG_PTB_Apo_2	65	72	PF02174	0.364
LIG_SH2_CRK	223	227	PF00017	0.346
LIG_SH2_CRK	286	290	PF00017	0.333
LIG_SH2_CRK	69	73	PF00017	0.295
LIG_SH2_GRB2like	163	166	PF00017	0.387
LIG_SH2_NCK_1	63	67	PF00017	0.342
LIG_SH2_SRC	63	66	PF00017	0.326
LIG_SH2_STAT5	182	185	PF00017	0.305
LIG_SH2_STAT5	254	257	PF00017	0.334
LIG_SH2_STAT5	299	302	PF00017	0.291
LIG_SH3_3	288	294	PF00018	0.362
LIG_SH3_3	304	310	PF00018	0.362
LIG_SH3_4	7	14	PF00018	0.724
LIG_SH3_5	294	298	PF00018	0.374
LIG_SUMO_SIM_anti_2	30	35	PF11976	0.422
LIG_SUMO_SIM_anti_2	346	352	PF11976	0.423
LIG_SUMO_SIM_par_1	27	32	PF11976	0.586
LIG_SUMO_SIM_par_1	36	42	PF11976	0.412
LIG_SUMO_SIM_par_1	46	52	PF11976	0.202
LIG_WW_1	108	111	PF00397	0.315
LIG_WW_3	224	228	PF00397	0.318
MOD_CDK_SPK_2	2	7	PF00069	0.575
MOD_CK1_1	101	107	PF00069	0.304
MOD_CK1_1	156	162	PF00069	0.324
MOD_CK1_1	180	186	PF00069	0.309
MOD_CK1_1	27	33	PF00069	0.597
MOD_CK2_1	262	268	PF00069	0.327
MOD_GlcNHglycan	154	158	PF01048	0.486
MOD_GlcNHglycan	236	239	PF01048	0.543
MOD_GlcNHglycan	242	245	PF01048	0.573
MOD_GlcNHglycan	351	354	PF01048	0.382
MOD_GlcNHglycan	51	54	PF01048	0.563
MOD_GSK3_1	180	187	PF00069	0.308
MOD_GSK3_1	193	200	PF00069	0.344
MOD_GSK3_1	306	313	PF00069	0.254
MOD_GSK3_1	49	56	PF00069	0.482
MOD_GSK3_1	81	88	PF00069	0.317
MOD_GSK3_1	89	96	PF00069	0.308
MOD_GSK3_1	98	105	PF00069	0.304
MOD_N-GLC_1	175	180	PF02516	0.485
MOD_N-GLC_1	184	189	PF02516	0.476
MOD_N-GLC_1	197	202	PF02516	0.532
MOD_N-GLC_1	240	245	PF02516	0.577
MOD_N-GLC_1	81	86	PF02516	0.506
MOD_N-GLC_2	206	208	PF02516	0.489
MOD_NEK2_1	125	130	PF00069	0.290
MOD_NEK2_1	175	180	PF00069	0.280
MOD_NEK2_1	184	189	PF00069	0.280
MOD_NEK2_1	197	202	PF00069	0.267
MOD_NEK2_1	29	34	PF00069	0.437
MOD_NEK2_1	349	354	PF00069	0.354
MOD_NEK2_1	85	90	PF00069	0.336
MOD_NEK2_1	98	103	PF00069	0.297
MOD_PKA_2	54	60	PF00069	0.349
MOD_Plk_1	175	181	PF00069	0.288
MOD_Plk_1	81	87	PF00069	0.285
MOD_Plk_2-3	54	60	PF00069	0.353
MOD_Plk_4	184	190	PF00069	0.352
MOD_Plk_4	29	35	PF00069	0.392
MOD_Plk_4	85	91	PF00069	0.388
MOD_ProDKin_1	104	110	PF00069	0.326
MOD_ProDKin_1	114	120	PF00069	0.364
MOD_ProDKin_1	2	8	PF00069	0.684
MOD_ProDKin_1	24	30	PF00069	0.582
MOD_ProDKin_1	306	312	PF00069	0.395
MOD_SUMO_rev_2	201	209	PF00179	0.372
TRG_ENDOCYTIC_2	123	126	PF00928	0.324
TRG_ENDOCYTIC_2	170	173	PF00928	0.265
TRG_ENDOCYTIC_2	286	289	PF00928	0.300
TRG_ENDOCYTIC_2	299	302	PF00928	0.271
TRG_ENDOCYTIC_2	63	66	PF00928	0.302
TRG_ENDOCYTIC_2	69	72	PF00928	0.294
TRG_ER_diArg_1	215	217	PF00400	0.207

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBG2	Leptomonas seymouri	32%	87%
A0A0N1PDQ9	Leptomonas seymouri	58%	100%
A0A0S4IMK4	Bodo saltans	31%	90%
A0A0S4J4T1	Bodo saltans	40%	96%
A0A0S4JB98	Bodo saltans	30%	90%
A0A0S4JJY0	Bodo saltans	28%	90%
A0A0S4JQR9	Bodo saltans	32%	69%
A0A1X0NHQ5	Trypanosomatidae	32%	76%
A0A1X0NV71	Trypanosomatidae	39%	96%
A0A1X0P5L3	Trypanosomatidae	32%	91%
A0A3R7LIN7	Trypanosoma rangeli	32%	85%
A0A3R7MGX3	Trypanosoma rangeli	41%	97%
A0A3S7X551	Leishmania donovani	73%	100%
A0A3S7X9N4	Leishmania donovani	29%	86%
A0A422NR22	Trypanosoma rangeli	35%	91%
A4HN07	Leishmania braziliensis	30%	86%
A4I7N1	Leishmania infantum	73%	100%
A4IBN0	Leishmania infantum	29%	86%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	94%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	77%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AFG4	Leishmania major	29%	100%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	86%
H2L0H3	Caenorhabditis elegans	22%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	93%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	88%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	92%
Q17QL5	Bos taurus	30%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	92%
Q2T9P5	Bos taurus	26%	100%
Q3MIR4	Homo sapiens	28%	100%
Q4Q5N2	Leishmania major	72%	100%
Q5F362	Gallus gallus	30%	98%
Q5R6C0	Pongo abelii	29%	100%
Q67YS6	Arabidopsis thaliana	23%	100%
Q6AY41	Rattus norvegicus	29%	100%
Q8BHG3	Mus musculus	27%	100%
Q8L8W0	Arabidopsis thaliana	25%	100%
Q8VEK0	Mus musculus	29%	100%
Q95JK4	Macaca fascicularis	25%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	98%
Q9D4D7	Mus musculus	25%	100%
Q9LTW0	Arabidopsis thaliana	26%	100%
Q9NV96	Homo sapiens	29%	100%
Q9SA35	Arabidopsis thaliana	26%	100%
Q9SLK2	Arabidopsis thaliana	26%	100%
V5AY40	Trypanosoma cruzi	33%	100%
V5B6A6	Trypanosoma cruzi	41%	96%
V5BY23	Trypanosoma cruzi	34%	91%