GP46-like surface antigen

Quick info Annotations Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, unknown function

Species:

Leishmania braziliensis

UniProt:

A4HJX1_LEIBR

TriTrypDb:

LbrM.31.3700 , LBRM2903_310026000

Length:

440

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 50

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 25, no: 2
NetGPI	no	yes: 0, no: 27

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	28
GO:0016020	membrane	2	10
GO:0042995	cell projection	2	28
GO:0043226	organelle	2	28
GO:0043227	membrane-bounded organelle	3	28
GO:0110165	cellular anatomical entity	1	28
GO:0120025	plasma membrane bounded cell projection	3	28

Expansion

Sequence features

A4HJX1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJX1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	219	223	PF00656	0.286
CLV_NRD_NRD_1	3	5	PF00675	0.711
CLV_PCSK_KEX2_1	3	5	PF00082	0.711
CLV_PCSK_KEX2_1	409	411	PF00082	0.558
CLV_PCSK_KEX2_1	45	47	PF00082	0.567
CLV_PCSK_PC1ET2_1	409	411	PF00082	0.558
CLV_PCSK_PC1ET2_1	45	47	PF00082	0.567
DEG_Nend_Nbox_1	1	3	PF02207	0.465
DEG_SPOP_SBC_1	291	295	PF00917	0.522
DEG_SPOP_SBC_1	299	303	PF00917	0.500
DEG_SPOP_SBC_1	307	311	PF00917	0.466
DEG_SPOP_SBC_1	315	319	PF00917	0.617
DEG_SPOP_SBC_1	323	327	PF00917	0.436
DEG_SPOP_SBC_1	331	335	PF00917	0.418
DEG_SPOP_SBC_1	339	343	PF00917	0.510
DEG_SPOP_SBC_1	347	351	PF00917	0.456
DEG_SPOP_SBC_1	355	359	PF00917	0.471
DEG_SPOP_SBC_1	363	367	PF00917	0.457
DOC_CYCLIN_yCln2_LP_2	23	29	PF00134	0.455
DOC_MAPK_MEF2A_6	148	157	PF00069	0.294
DOC_PP1_RVXF_1	172	179	PF00149	0.253
DOC_PP2B_LxvP_1	23	26	PF13499	0.498
DOC_USP7_MATH_1	75	79	PF00917	0.274
DOC_USP7_MATH_2	215	221	PF00917	0.261
LIG_14-3-3_CanoR_1	96	100	PF00244	0.248
LIG_Actin_WH2_2	159	176	PF00022	0.252
LIG_BIR_III_2	74	78	PF00653	0.322
LIG_BRCT_BRCA1_1	420	424	PF00533	0.211
LIG_BRCT_BRCA1_1	53	57	PF00533	0.294
LIG_FHA_1	137	143	PF00498	0.329
LIG_FHA_1	199	205	PF00498	0.263
LIG_FHA_1	222	228	PF00498	0.298
LIG_FHA_1	233	239	PF00498	0.342
LIG_FHA_1	301	307	PF00498	0.438
LIG_FHA_1	349	355	PF00498	0.549
LIG_FHA_1	425	431	PF00498	0.204
LIG_FHA_1	61	67	PF00498	0.413
LIG_FHA_2	109	115	PF00498	0.437
LIG_FHA_2	366	372	PF00498	0.452
LIG_FHA_2	388	394	PF00498	0.294
LIG_FHA_2	80	86	PF00498	0.368
LIG_LIR_Gen_1	118	129	PF02991	0.408
LIG_LIR_Gen_1	169	178	PF02991	0.335
LIG_LIR_Gen_1	193	200	PF02991	0.286
LIG_LIR_Gen_1	217	226	PF02991	0.296
LIG_LIR_Gen_1	240	248	PF02991	0.371
LIG_LIR_Gen_1	270	278	PF02991	0.278
LIG_LIR_Nem_3	118	124	PF02991	0.394
LIG_LIR_Nem_3	144	149	PF02991	0.319
LIG_LIR_Nem_3	169	173	PF02991	0.294
LIG_LIR_Nem_3	270	274	PF02991	0.316
LIG_LIR_Nem_3	427	432	PF02991	0.199
LIG_REV1ctd_RIR_1	129	139	PF16727	0.268
LIG_SH2_CRK	121	125	PF00017	0.279
LIG_SH2_GRB2like	121	124	PF00017	0.296
LIG_SH2_GRB2like	277	280	PF00017	0.268
LIG_SH2_SRC	277	280	PF00017	0.268
LIG_SH2_STAP1	121	125	PF00017	0.277
LIG_SH2_STAP1	277	281	PF00017	0.263
LIG_SH2_STAP1	53	57	PF00017	0.294
LIG_SH2_STAT3	206	209	PF00017	0.272
LIG_SH2_STAT3	225	228	PF00017	0.244
LIG_SH2_STAT5	225	228	PF00017	0.271
LIG_SH2_STAT5	399	402	PF00017	0.347
LIG_SH3_3	110	116	PF00018	0.419
LIG_SH3_3	38	44	PF00018	0.375
LIG_SUMO_SIM_par_1	433	439	PF11976	0.203
LIG_TYR_ITIM	119	124	PF00017	0.358
LIG_UBA3_1	125	134	PF00899	0.245
LIG_UBA3_1	245	250	PF00899	0.252
MOD_CK1_1	169	175	PF00069	0.384
MOD_CK1_1	193	199	PF00069	0.389
MOD_CK1_1	220	226	PF00069	0.418
MOD_CK1_1	366	372	PF00069	0.457
MOD_CK1_1	62	68	PF00069	0.346
MOD_CK2_1	177	183	PF00069	0.343
MOD_CK2_1	234	240	PF00069	0.300
MOD_CK2_1	365	371	PF00069	0.469
MOD_CK2_1	53	59	PF00069	0.407
MOD_CK2_1	79	85	PF00069	0.299
MOD_CK2_1	94	100	PF00069	0.299
MOD_GlcNHglycan	162	165	PF01048	0.524
MOD_GlcNHglycan	186	189	PF01048	0.598
MOD_GlcNHglycan	210	213	PF01048	0.563
MOD_GlcNHglycan	279	282	PF01048	0.497
MOD_GlcNHglycan	396	399	PF01048	0.504
MOD_GSK3_1	138	145	PF00069	0.334
MOD_GSK3_1	156	163	PF00069	0.351
MOD_GSK3_1	177	184	PF00069	0.388
MOD_GSK3_1	186	193	PF00069	0.392
MOD_GSK3_1	216	223	PF00069	0.397
MOD_GSK3_1	234	241	PF00069	0.404
MOD_GSK3_1	289	296	PF00069	0.437
MOD_GSK3_1	298	305	PF00069	0.477
MOD_GSK3_1	306	313	PF00069	0.472
MOD_GSK3_1	314	321	PF00069	0.457
MOD_GSK3_1	322	329	PF00069	0.453
MOD_GSK3_1	330	337	PF00069	0.508
MOD_GSK3_1	338	345	PF00069	0.601
MOD_GSK3_1	346	353	PF00069	0.511
MOD_GSK3_1	354	361	PF00069	0.576
MOD_GSK3_1	362	369	PF00069	0.647
MOD_GSK3_1	418	425	PF00069	0.217
MOD_GSK3_1	51	58	PF00069	0.313
MOD_GSK3_1	75	82	PF00069	0.292
MOD_N-GLC_1	267	272	PF02516	0.480
MOD_N-GLC_2	288	290	PF02516	0.663
MOD_NEK2_1	101	106	PF00069	0.366
MOD_NEK2_1	125	130	PF00069	0.327
MOD_NEK2_1	157	162	PF00069	0.362
MOD_NEK2_1	210	215	PF00069	0.345
MOD_NEK2_1	221	226	PF00069	0.363
MOD_NEK2_1	234	239	PF00069	0.394
MOD_NEK2_1	424	429	PF00069	0.199
MOD_NEK2_1	79	84	PF00069	0.376
MOD_PKA_2	95	101	PF00069	0.257
MOD_Plk_1	216	222	PF00069	0.281
MOD_Plk_1	267	273	PF00069	0.301
MOD_Plk_2-3	217	223	PF00069	0.255
MOD_Plk_4	199	205	PF00069	0.373
MOD_Plk_4	221	227	PF00069	0.260
MOD_Plk_4	249	255	PF00069	0.294
MOD_Plk_4	425	431	PF00069	0.228
TRG_ENDOCYTIC_2	121	124	PF00928	0.437
TRG_ER_diArg_1	2	4	PF00400	0.633

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I121	Leptomonas seymouri	26%	88%
A0A0S4IQI0	Bodo saltans	29%	75%
A0A3Q8I9A6	Leishmania donovani	37%	100%
A0A3Q8IFC2	Leishmania donovani	66%	100%
A0A3Q8IIJ9	Leishmania donovani	34%	100%
A0A3S7X4J4	Leishmania donovani	34%	100%
A4H4D2	Leishmania braziliensis	26%	100%
A4HJC8	Leishmania braziliensis	35%	100%
A4HVB0	Leishmania infantum	38%	100%
A4I6S4	Leishmania infantum	35%	100%
D1GJ51	Leishmania infantum	31%	95%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9AGH0	Leishmania infantum	41%	100%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	65%	100%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
Q25331	Leishmania major	37%	100%
Q4Q6B6	Leishmania major	34%	100%
Q4Q6B7	Leishmania major	34%	100%
Q4Q6B8	Leishmania major	38%	83%
Q4QGJ4	Leishmania major	37%	100%
Q4QGJ6	Leishmania major	33%	77%
Q4QGK6	Leishmania major	37%	100%
Q4QGL4	Leishmania major	36%	100%