GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania braziliensis

UniProt:

A4HJR1_LEIBR

TriTrypDb:

LbrM.31.3080 , LBRM2903_310040600

Length:

264

Annotations

LeishMANIAdb annotations

Related to other Eukaryotic GOLD domain proteins, involved in sorting of GPI-achored proteins. Kinetoplastids contain several paralogous GOLD domain protein families, not all closely related to each others.. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 9, no: 3
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Expansion

Sequence features

A4HJR1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJR1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	5	7	PF00675	0.694
CLV_PCSK_KEX2_1	167	169	PF00082	0.475
CLV_PCSK_KEX2_1	5	7	PF00082	0.694
CLV_PCSK_PC1ET2_1	167	169	PF00082	0.480
DEG_APCC_DBOX_1	213	221	PF00400	0.338
DEG_ODPH_VHL_1	13	26	PF01847	0.609
DOC_MAPK_gen_1	167	173	PF00069	0.318
DOC_PP1_RVXF_1	157	164	PF00149	0.280
DOC_PP4_FxxP_1	30	33	PF00568	0.362
DOC_SPAK_OSR1_1	162	166	PF12202	0.353
DOC_WW_Pin1_4	154	159	PF00397	0.247
DOC_WW_Pin1_4	29	34	PF00397	0.526
DOC_WW_Pin1_4	83	88	PF00397	0.305
LIG_14-3-3_CanoR_1	121	129	PF00244	0.286
LIG_14-3-3_CanoR_1	68	75	PF00244	0.267
LIG_BIR_III_4	176	180	PF00653	0.173
LIG_BRCT_BRCA1_1	145	149	PF00533	0.353
LIG_BRCT_BRCA1_1	193	197	PF00533	0.277
LIG_FHA_1	52	58	PF00498	0.315
LIG_FHA_1	64	70	PF00498	0.256
LIG_FHA_1	84	90	PF00498	0.117
LIG_LIR_Apic_2	126	132	PF02991	0.249
LIG_LIR_Gen_1	150	161	PF02991	0.271
LIG_LIR_Gen_1	240	251	PF02991	0.195
LIG_LIR_Gen_1	50	59	PF02991	0.336
LIG_LIR_Nem_3	150	156	PF02991	0.254
LIG_LIR_Nem_3	157	163	PF02991	0.221
LIG_LIR_Nem_3	240	246	PF02991	0.316
LIG_LIR_Nem_3	50	56	PF02991	0.327
LIG_NRBOX	22	28	PF00104	0.574
LIG_Pex14_2	149	153	PF04695	0.267
LIG_SH2_CRK	129	133	PF00017	0.256
LIG_SH2_CRK	192	196	PF00017	0.309
LIG_SH2_CRK	241	245	PF00017	0.341
LIG_SH2_NCK_1	129	133	PF00017	0.256
LIG_SH2_PTP2	243	246	PF00017	0.409
LIG_SH2_SRC	241	244	PF00017	0.448
LIG_SH2_STAP1	127	131	PF00017	0.338
LIG_SH2_STAP1	53	57	PF00017	0.312
LIG_SH2_STAT5	127	130	PF00017	0.258
LIG_SH2_STAT5	243	246	PF00017	0.340
LIG_SH2_STAT5	53	56	PF00017	0.338
LIG_Sin3_3	226	233	PF02671	0.234
LIG_SUMO_SIM_par_1	232	237	PF11976	0.421
LIG_SUMO_SIM_par_1	32	37	PF11976	0.582
LIG_TRAF2_1	184	187	PF00917	0.336
MOD_CDK_SPK_2	154	159	PF00069	0.247
MOD_CK1_1	152	158	PF00069	0.269
MOD_CK1_1	32	38	PF00069	0.430
MOD_CK2_1	40	46	PF00069	0.302
MOD_GSK3_1	111	118	PF00069	0.329
MOD_GSK3_1	123	130	PF00069	0.267
MOD_GSK3_1	47	54	PF00069	0.287
MOD_GSK3_1	59	66	PF00069	0.220
MOD_GSK3_1	89	96	PF00069	0.296
MOD_NEK2_1	149	154	PF00069	0.267
MOD_NEK2_1	259	264	PF00069	0.704
MOD_NEK2_1	63	68	PF00069	0.289
MOD_PK_1	191	197	PF00069	0.277
MOD_PKA_2	111	117	PF00069	0.311
MOD_PKA_2	120	126	PF00069	0.284
MOD_PKA_2	67	73	PF00069	0.267
MOD_Plk_1	143	149	PF00069	0.267
MOD_Plk_1	93	99	PF00069	0.285
MOD_Plk_4	111	117	PF00069	0.261
MOD_Plk_4	130	136	PF00069	0.277
MOD_Plk_4	191	197	PF00069	0.281
MOD_Plk_4	219	225	PF00069	0.205
MOD_ProDKin_1	154	160	PF00069	0.247
MOD_ProDKin_1	29	35	PF00069	0.528
MOD_ProDKin_1	83	89	PF00069	0.305
TRG_ENDOCYTIC_2	192	195	PF00928	0.309
TRG_ENDOCYTIC_2	241	244	PF00928	0.323
TRG_ENDOCYTIC_2	53	56	PF00928	0.334
TRG_ER_diArg_1	135	138	PF00400	0.326
TRG_ER_diArg_1	4	6	PF00400	0.612
TRG_Pf-PMV_PEXEL_1	248	253	PF00026	0.419

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4P8	Leptomonas seymouri	76%	100%
A0A0S4JHN1	Bodo saltans	30%	100%
A0A0S4JHW2	Bodo saltans	30%	100%
A0A1X0NIF0	Trypanosomatidae	52%	100%
A0A3Q8IGK5	Leishmania donovani	84%	100%
A0A3R7MK91	Trypanosoma rangeli	47%	100%
A4I778	Leishmania infantum	84%	100%
C9ZWM6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9B272	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
O17528	Caenorhabditis elegans	24%	100%
Q4Q5Y7	Leishmania major	84%	100%
V5B7K8	Trypanosoma cruzi	47%	100%