Putative 3'-nucleotidase/nuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3'-nucleotidase/nuclease precursor, putative

Species:

Leishmania braziliensis

UniProt:

A4HJL3_LEIBR

TriTrypDb:

LbrM.31.2580 , LBRM2903_310035300

Length:

379

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 22, no: 6
NetGPI	no	yes: 0, no: 28

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0009986	cell surface	2	1

Expansion

Sequence features

A4HJL3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJL3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	29
GO:0006259	DNA metabolic process	4	29
GO:0006308	DNA catabolic process	5	29
GO:0006725	cellular aromatic compound metabolic process	3	29
GO:0006807	nitrogen compound metabolic process	2	29
GO:0008152	metabolic process	1	29
GO:0009056	catabolic process	2	29
GO:0009057	macromolecule catabolic process	4	29
GO:0009987	cellular process	1	29
GO:0019439	aromatic compound catabolic process	4	29
GO:0034641	cellular nitrogen compound metabolic process	3	29
GO:0034655	nucleobase-containing compound catabolic process	4	29
GO:0043170	macromolecule metabolic process	3	29
GO:0044237	cellular metabolic process	2	29
GO:0044238	primary metabolic process	2	29
GO:0044248	cellular catabolic process	3	29
GO:0044260	obsolete cellular macromolecule metabolic process	3	29
GO:0044265	obsolete cellular macromolecule catabolic process	4	29
GO:0044270	cellular nitrogen compound catabolic process	4	29
GO:0046483	heterocycle metabolic process	3	29
GO:0046700	heterocycle catabolic process	4	29
GO:0071704	organic substance metabolic process	2	29
GO:0090304	nucleic acid metabolic process	4	29
GO:1901360	organic cyclic compound metabolic process	3	29
GO:1901361	organic cyclic compound catabolic process	4	29
GO:1901575	organic substance catabolic process	3	29
GO:0006163	purine nucleotide metabolic process	5	1
GO:0006195	purine nucleotide catabolic process	6	1
GO:0006196	AMP catabolic process	7	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009123	nucleoside monophosphate metabolic process	5	1
GO:0009125	nucleoside monophosphate catabolic process	6	1
GO:0009126	purine nucleoside monophosphate metabolic process	6	1
GO:0009128	purine nucleoside monophosphate catabolic process	7	1
GO:0009150	purine ribonucleotide metabolic process	6	1
GO:0009154	purine ribonucleotide catabolic process	6	1
GO:0009158	ribonucleoside monophosphate catabolic process	7	1
GO:0009161	ribonucleoside monophosphate metabolic process	6	1
GO:0009166	nucleotide catabolic process	6	1
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	1
GO:0009169	purine ribonucleoside monophosphate catabolic process	8	1
GO:0009259	ribonucleotide metabolic process	5	1
GO:0009261	ribonucleotide catabolic process	5	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019693	ribose phosphate metabolic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046033	AMP metabolic process	7	1
GO:0046434	organophosphate catabolic process	4	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:0072523	purine-containing compound catabolic process	5	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901136	carbohydrate derivative catabolic process	4	1
GO:1901292	nucleoside phosphate catabolic process	5	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	29
GO:0003824	catalytic activity	1	29
GO:0004518	nuclease activity	4	29
GO:0004519	endonuclease activity	5	29
GO:0005488	binding	1	29
GO:0016787	hydrolase activity	2	29
GO:0016788	hydrolase activity, acting on ester bonds	3	29
GO:0043167	ion binding	2	29
GO:0043169	cation binding	3	29
GO:0046872	metal ion binding	4	29
GO:0097159	organic cyclic compound binding	2	29
GO:1901363	heterocyclic compound binding	2	29
GO:0008252	nucleotidase activity	6	1
GO:0008254	3'-nucleotidase activity	7	1
GO:0016791	phosphatase activity	5	1
GO:0042578	phosphoric ester hydrolase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	231	235	PF00656	0.355
CLV_PCSK_KEX2_1	369	371	PF00082	0.386
CLV_PCSK_PC1ET2_1	369	371	PF00082	0.349
CLV_PCSK_SKI1_1	186	190	PF00082	0.542
CLV_PCSK_SKI1_1	273	277	PF00082	0.443
CLV_PCSK_SKI1_1	281	285	PF00082	0.434
CLV_PCSK_SKI1_1	289	293	PF00082	0.445
DOC_CKS1_1	95	100	PF01111	0.264
DOC_CYCLIN_RxL_1	51	62	PF00134	0.206
DOC_MAPK_gen_1	4	12	PF00069	0.414
DOC_MAPK_MEF2A_6	31	38	PF00069	0.355
DOC_PP1_RVXF_1	80	87	PF00149	0.160
DOC_USP7_MATH_1	101	105	PF00917	0.164
DOC_USP7_MATH_1	360	364	PF00917	0.310
DOC_USP7_UBL2_3	237	241	PF12436	0.261
DOC_USP7_UBL2_3	272	276	PF12436	0.292
DOC_WW_Pin1_4	107	112	PF00397	0.280
DOC_WW_Pin1_4	65	70	PF00397	0.315
DOC_WW_Pin1_4	94	99	PF00397	0.266
LIG_Actin_WH2_2	286	301	PF00022	0.235
LIG_BIR_II_1	1	5	PF00653	0.643
LIG_EH1_1	350	358	PF00400	0.187
LIG_FHA_1	104	110	PF00498	0.349
LIG_FHA_1	112	118	PF00498	0.309
LIG_FHA_1	247	253	PF00498	0.262
LIG_FHA_1	337	343	PF00498	0.350
LIG_FHA_1	353	359	PF00498	0.330
LIG_FHA_2	225	231	PF00498	0.359
LIG_FHA_2	264	270	PF00498	0.348
LIG_GBD_Chelix_1	290	298	PF00786	0.487
LIG_LIR_Apic_2	97	103	PF02991	0.160
LIG_LIR_Gen_1	212	221	PF02991	0.287
LIG_LIR_Gen_1	90	100	PF02991	0.316
LIG_LIR_Nem_3	212	217	PF02991	0.287
LIG_LIR_Nem_3	219	223	PF02991	0.284
LIG_LIR_Nem_3	90	95	PF02991	0.323
LIG_MAD2	292	300	PF02301	0.160
LIG_PCNA_yPIPBox_3	322	332	PF02747	0.327
LIG_Pex14_2	347	351	PF04695	0.323
LIG_PTB_Apo_2	244	251	PF02174	0.292
LIG_PTB_Phospho_1	244	250	PF10480	0.292
LIG_SH2_CRK	214	218	PF00017	0.244
LIG_SH2_CRK	256	260	PF00017	0.256
LIG_SH2_CRK	374	378	PF00017	0.519
LIG_SH2_SRC	223	226	PF00017	0.359
LIG_SH2_SRC	256	259	PF00017	0.234
LIG_SH2_STAP1	228	232	PF00017	0.320
LIG_SH2_STAT3	328	331	PF00017	0.326
LIG_SH2_STAT5	223	226	PF00017	0.272
LIG_SH2_STAT5	329	332	PF00017	0.436
LIG_SH3_1	370	376	PF00018	0.513
LIG_SH3_3	3	9	PF00018	0.509
LIG_SH3_3	370	376	PF00018	0.533
LIG_SH3_3	63	69	PF00018	0.252
LIG_SH3_3	95	101	PF00018	0.269
LIG_Sin3_3	14	21	PF02671	0.165
LIG_SUMO_SIM_anti_2	141	146	PF11976	0.206
LIG_SUMO_SIM_par_1	104	110	PF11976	0.357
LIG_SUMO_SIM_par_1	57	62	PF11976	0.167
MOD_CK1_1	182	188	PF00069	0.392
MOD_CK1_1	246	252	PF00069	0.387
MOD_CK1_1	87	93	PF00069	0.181
MOD_CK2_1	192	198	PF00069	0.347
MOD_CK2_1	213	219	PF00069	0.309
MOD_CK2_1	44	50	PF00069	0.323
MOD_GlcNHglycan	25	28	PF01048	0.620
MOD_GlcNHglycan	301	304	PF01048	0.500
MOD_GlcNHglycan	334	337	PF01048	0.561
MOD_GlcNHglycan	61	64	PF01048	0.446
MOD_GlcNHglycan	86	89	PF01048	0.456
MOD_GSK3_1	103	110	PF00069	0.258
MOD_GSK3_1	224	231	PF00069	0.261
MOD_GSK3_1	23	30	PF00069	0.499
MOD_GSK3_1	312	319	PF00069	0.408
MOD_GSK3_1	332	339	PF00069	0.440
MOD_GSK3_1	84	91	PF00069	0.217
MOD_N-GLC_1	246	251	PF02516	0.456
MOD_N-GLC_1	294	299	PF02516	0.555
MOD_N-GLC_1	321	326	PF02516	0.570
MOD_NEK2_1	123	128	PF00069	0.330
MOD_NEK2_1	224	229	PF00069	0.287
MOD_NEK2_1	294	299	PF00069	0.305
MOD_NEK2_1	44	49	PF00069	0.272
MOD_NEK2_1	59	64	PF00069	0.220
MOD_Plk_1	224	230	PF00069	0.266
MOD_Plk_1	246	252	PF00069	0.284
MOD_Plk_1	294	300	PF00069	0.321
MOD_Plk_1	321	327	PF00069	0.530
MOD_Plk_1	44	50	PF00069	0.294
MOD_Plk_2-3	213	219	PF00069	0.177
MOD_Plk_2-3	230	236	PF00069	0.198
MOD_Plk_2-3	263	269	PF00069	0.160
MOD_Plk_4	116	122	PF00069	0.251
MOD_Plk_4	352	358	PF00069	0.245
MOD_Plk_4	44	50	PF00069	0.302
MOD_ProDKin_1	107	113	PF00069	0.280
MOD_ProDKin_1	65	71	PF00069	0.315
MOD_ProDKin_1	94	100	PF00069	0.266
MOD_SUMO_rev_2	79	84	PF00179	0.286
TRG_DiLeu_BaEn_1	280	285	PF01217	0.235
TRG_ENDOCYTIC_2	214	217	PF00928	0.274
TRG_ENDOCYTIC_2	374	377	PF00928	0.568
TRG_Pf-PMV_PEXEL_1	207	212	PF00026	0.523

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Q7	Leptomonas seymouri	37%	100%
A0A0S4JAL3	Bodo saltans	43%	100%
A0A0S4JML7	Bodo saltans	23%	100%
A0A1X0P2K7	Trypanosomatidae	43%	100%
A0A3Q8IBF6	Leishmania donovani	47%	99%
A0A3Q8IEW3	Leishmania donovani	39%	100%
A0A3Q8IFH4	Leishmania donovani	76%	100%
A0A3Q8IK90	Leishmania donovani	64%	100%
A0A3S7X3A6	Leishmania donovani	39%	100%
A0A422N7B0	Trypanosoma rangeli	42%	100%
A4H6V5	Leishmania braziliensis	45%	100%
A4HI96	Leishmania braziliensis	35%	100%
A4I5I0	Leishmania infantum	38%	100%
A4I736	Leishmania infantum	76%	100%
C9ZQH5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
C9ZQH6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
C9ZQW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AGC7	Leishmania infantum	47%	99%
E9ANW7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	46%	99%
E9B0S4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B0S5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9B231	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	100%
F4JJL0	Arabidopsis thaliana	30%	100%
F4JJL3	Arabidopsis thaliana	30%	100%
P24021	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	26%	100%
Q4Q630	Leishmania major	73%	100%
Q4Q7F3	Leishmania major	39%	100%
Q4Q7F4	Leishmania major	39%	100%
Q4QGQ3	Leishmania major	45%	100%
Q66VY6	Leishmania major	62%	100%
Q8LDW6	Arabidopsis thaliana	30%	100%
Q9C9G4	Arabidopsis thaliana	27%	100%
Q9SXA6	Arabidopsis thaliana	27%	100%
V5B8Z9	Trypanosoma cruzi	42%	98%