Putative aminopeptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

metallo-peptidase, Clan MA(E), Family M1

Species:

Leishmania braziliensis

UniProt:

A4HJK9_LEIBR

TriTrypDb:

LbrM.31.2530 , LBRM2903_310034700

Length:

618

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HJK9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJK9

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	1
GO:0006518	peptide metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0019538	protein metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043171	peptide catabolic process	4	1
GO:0043603	amide metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004177	aminopeptidase activity	5	10
GO:0005488	binding	1	11
GO:0008233	peptidase activity	3	11
GO:0008237	metallopeptidase activity	4	11
GO:0008238	exopeptidase activity	4	10
GO:0008270	zinc ion binding	6	11
GO:0016787	hydrolase activity	2	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0008235	metalloexopeptidase activity	5	1
GO:0033218	amide binding	2	1
GO:0042277	peptide binding	3	1
GO:0070006	metalloaminopeptidase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	175	179	PF00656	0.602
CLV_C14_Caspase3-7	65	69	PF00656	0.768
CLV_NRD_NRD_1	288	290	PF00675	0.419
CLV_NRD_NRD_1	366	368	PF00675	0.486
CLV_NRD_NRD_1	43	45	PF00675	0.444
CLV_NRD_NRD_1	501	503	PF00675	0.224
CLV_NRD_NRD_1	593	595	PF00675	0.409
CLV_NRD_NRD_1	79	81	PF00675	0.475
CLV_PCSK_FUR_1	41	45	PF00082	0.448
CLV_PCSK_FUR_1	77	81	PF00082	0.521
CLV_PCSK_KEX2_1	163	165	PF00082	0.676
CLV_PCSK_KEX2_1	366	368	PF00082	0.486
CLV_PCSK_KEX2_1	43	45	PF00082	0.444
CLV_PCSK_KEX2_1	593	595	PF00082	0.409
CLV_PCSK_KEX2_1	79	81	PF00082	0.519
CLV_PCSK_PC1ET2_1	163	165	PF00082	0.728
CLV_PCSK_PC7_1	362	368	PF00082	0.486
CLV_PCSK_SKI1_1	101	105	PF00082	0.515
CLV_PCSK_SKI1_1	190	194	PF00082	0.393
CLV_PCSK_SKI1_1	251	255	PF00082	0.314
CLV_PCSK_SKI1_1	290	294	PF00082	0.381
CLV_PCSK_SKI1_1	370	374	PF00082	0.521
CLV_PCSK_SKI1_1	563	567	PF00082	0.259
CLV_PCSK_SKI1_1	608	612	PF00082	0.403
CLV_PCSK_SKI1_1	85	89	PF00082	0.482
CLV_Separin_Metazoa	590	594	PF03568	0.386
DEG_APCC_DBOX_1	607	615	PF00400	0.296
DEG_SPOP_SBC_1	519	523	PF00917	0.323
DEG_SPOP_SBC_1	55	59	PF00917	0.505
DOC_CYCLIN_RxL_1	375	385	PF00134	0.470
DOC_CYCLIN_yCln2_LP_2	474	480	PF00134	0.259
DOC_MAPK_gen_1	593	601	PF00069	0.391
DOC_PP1_RVXF_1	188	195	PF00149	0.358
DOC_PP1_RVXF_1	261	267	PF00149	0.386
DOC_PP2B_LxvP_1	341	344	PF13499	0.449
DOC_PP2B_LxvP_1	474	477	PF13499	0.259
DOC_PP4_FxxP_1	19	22	PF00568	0.434
DOC_PP4_FxxP_1	254	257	PF00568	0.290
DOC_PP4_FxxP_1	394	397	PF00568	0.290
DOC_PP4_FxxP_1	514	517	PF00568	0.313
DOC_USP7_MATH_1	141	145	PF00917	0.749
DOC_USP7_MATH_1	158	162	PF00917	0.786
DOC_USP7_MATH_1	215	219	PF00917	0.331
DOC_USP7_MATH_1	440	444	PF00917	0.290
DOC_USP7_MATH_1	50	54	PF00917	0.572
DOC_USP7_MATH_1	517	521	PF00917	0.321
DOC_USP7_MATH_1	613	617	PF00917	0.476
DOC_USP7_MATH_1	83	87	PF00917	0.294
DOC_USP7_UBL2_3	499	503	PF12436	0.308
DOC_WW_Pin1_4	114	119	PF00397	0.457
DOC_WW_Pin1_4	178	183	PF00397	0.568
DOC_WW_Pin1_4	253	258	PF00397	0.208
DOC_WW_Pin1_4	513	518	PF00397	0.390
DOC_WW_Pin1_4	599	604	PF00397	0.524
DOC_WW_Pin1_4	6	11	PF00397	0.503
LIG_14-3-3_CanoR_1	370	379	PF00244	0.447
LIG_APCC_ABBA_1	200	205	PF00400	0.393
LIG_BIR_II_1	1	5	PF00653	0.510
LIG_BRCT_BRCA1_1	510	514	PF00533	0.313
LIG_Clathr_ClatBox_1	191	195	PF01394	0.470
LIG_CSL_BTD_1	7	10	PF09270	0.240
LIG_deltaCOP1_diTrp_1	186	194	PF00928	0.468
LIG_FHA_1	12	18	PF00498	0.375
LIG_FHA_1	122	128	PF00498	0.502
LIG_FHA_1	234	240	PF00498	0.452
LIG_FHA_1	35	41	PF00498	0.420
LIG_FHA_1	371	377	PF00498	0.494
LIG_FHA_1	456	462	PF00498	0.425
LIG_FHA_1	521	527	PF00498	0.347
LIG_FHA_2	173	179	PF00498	0.677
LIG_FHA_2	241	247	PF00498	0.290
LIG_FHA_2	280	286	PF00498	0.419
LIG_LIR_Apic_2	511	517	PF02991	0.313
LIG_LIR_Gen_1	275	281	PF02991	0.218
LIG_LIR_Gen_1	314	322	PF02991	0.535
LIG_LIR_Gen_1	558	568	PF02991	0.344
LIG_LIR_Gen_1	70	76	PF02991	0.573
LIG_LIR_Nem_3	275	280	PF02991	0.335
LIG_LIR_Nem_3	314	318	PF02991	0.370
LIG_LIR_Nem_3	558	564	PF02991	0.331
LIG_LIR_Nem_3	70	75	PF02991	0.537
LIG_NRBOX	405	411	PF00104	0.383
LIG_NRBOX	567	573	PF00104	0.208
LIG_PAM2_1	178	190	PF00658	0.378
LIG_PDZ_Class_1	613	618	PF00595	0.505
LIG_Pex14_2	245	249	PF04695	0.393
LIG_SH2_GRB2like	72	75	PF00017	0.347
LIG_SH2_SRC	545	548	PF00017	0.419
LIG_SH2_STAP1	72	76	PF00017	0.550
LIG_SH2_STAT5	311	314	PF00017	0.352
LIG_SH2_STAT5	416	419	PF00017	0.324
LIG_SH2_STAT5	47	50	PF00017	0.384
LIG_SH2_STAT5	6	9	PF00017	0.275
LIG_SH2_STAT5	604	607	PF00017	0.354
LIG_SH3_3	254	260	PF00018	0.358
LIG_SH3_3	301	307	PF00018	0.337
LIG_SH3_3	409	415	PF00018	0.328
LIG_SH3_3	573	579	PF00018	0.505
LIG_SH3_4	499	506	PF00018	0.252
LIG_SUMO_SIM_anti_2	332	338	PF11976	0.539
LIG_SUMO_SIM_anti_2	404	410	PF11976	0.312
LIG_SUMO_SIM_par_1	128	133	PF11976	0.529
LIG_WRC_WIRS_1	216	221	PF05994	0.419
LIG_WRC_WIRS_1	312	317	PF05994	0.432
MOD_CK1_1	144	150	PF00069	0.665
MOD_CK1_1	323	329	PF00069	0.461
MOD_CK1_1	352	358	PF00069	0.552
MOD_CK1_1	508	514	PF00069	0.401
MOD_CK1_1	518	524	PF00069	0.285
MOD_CK1_1	53	59	PF00069	0.718
MOD_CK1_1	60	66	PF00069	0.701
MOD_CK2_1	240	246	PF00069	0.290
MOD_CK2_1	279	285	PF00069	0.419
MOD_CK2_1	415	421	PF00069	0.290
MOD_CK2_1	448	454	PF00069	0.259
MOD_CK2_1	603	609	PF00069	0.295
MOD_Cter_Amidation	287	290	PF01082	0.419
MOD_GlcNHglycan	118	121	PF01048	0.609
MOD_GlcNHglycan	149	152	PF01048	0.635
MOD_GlcNHglycan	175	178	PF01048	0.602
MOD_GlcNHglycan	31	34	PF01048	0.388
MOD_GlcNHglycan	327	330	PF01048	0.663
MOD_GlcNHglycan	417	420	PF01048	0.290
MOD_GlcNHglycan	507	510	PF01048	0.312
MOD_GlcNHglycan	549	552	PF01048	0.321
MOD_GlcNHglycan	65	68	PF01048	0.746
MOD_GlcNHglycan	84	88	PF01048	0.247
MOD_GSK3_1	141	148	PF00069	0.677
MOD_GSK3_1	229	236	PF00069	0.405
MOD_GSK3_1	272	279	PF00069	0.309
MOD_GSK3_1	320	327	PF00069	0.684
MOD_GSK3_1	348	355	PF00069	0.454
MOD_GSK3_1	50	57	PF00069	0.732
MOD_GSK3_1	513	520	PF00069	0.310
MOD_GSK3_1	527	534	PF00069	0.386
MOD_GSK3_1	58	65	PF00069	0.706
MOD_GSK3_1	599	606	PF00069	0.584
MOD_N-GLC_1	428	433	PF02516	0.225
MOD_N-GLC_1	53	58	PF02516	0.496
MOD_NEK2_1	17	22	PF00069	0.423
MOD_NEK2_1	29	34	PF00069	0.382
MOD_NEK2_1	298	303	PF00069	0.383
MOD_NEK2_1	324	329	PF00069	0.680
MOD_NEK2_1	428	433	PF00069	0.225
MOD_PK_1	128	134	PF00069	0.524
MOD_PKA_2	144	150	PF00069	0.516
MOD_PKA_2	227	233	PF00069	0.208
MOD_PKA_2	380	386	PF00069	0.458
MOD_Plk_1	206	212	PF00069	0.308
MOD_Plk_1	428	434	PF00069	0.225
MOD_Plk_1	83	89	PF00069	0.283
MOD_Plk_2-3	320	326	PF00069	0.421
MOD_Plk_4	276	282	PF00069	0.214
MOD_Plk_4	35	41	PF00069	0.487
MOD_Plk_4	422	428	PF00069	0.396
MOD_Plk_4	492	498	PF00069	0.492
MOD_ProDKin_1	114	120	PF00069	0.474
MOD_ProDKin_1	178	184	PF00069	0.555
MOD_ProDKin_1	253	259	PF00069	0.208
MOD_ProDKin_1	513	519	PF00069	0.390
MOD_ProDKin_1	599	605	PF00069	0.532
MOD_ProDKin_1	6	12	PF00069	0.504
MOD_SUMO_rev_2	430	440	PF00179	0.208
TRG_DiLeu_BaEn_2	70	76	PF01217	0.570
TRG_DiLeu_BaLyEn_6	396	401	PF01217	0.393
TRG_DiLeu_LyEn_5	237	242	PF01217	0.383
TRG_ENDOCYTIC_2	72	75	PF00928	0.558
TRG_ER_diArg_1	42	44	PF00400	0.429
TRG_ER_diArg_1	592	594	PF00400	0.381
TRG_ER_diArg_1	76	79	PF00400	0.521
TRG_NLS_MonoExtC_3	501	507	PF00514	0.225
TRG_NLS_MonoExtN_4	499	506	PF00514	0.225
TRG_Pf-PMV_PEXEL_1	80	84	PF00026	0.474

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC92	Leptomonas seymouri	55%	100%
A0A1X0NRQ5	Trypanosomatidae	37%	100%
A0A3S7X4Q1	Leishmania donovani	75%	100%
A0A422N3Z8	Trypanosoma rangeli	35%	100%
A4I709	Leishmania infantum	75%	100%
C9ZWP4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	95%
E9B227	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	100%
Q4Q635	Leishmania major	74%	100%
V5AMB1	Trypanosoma cruzi	35%	100%