Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

metallo-peptidase, Clan MA(M), Family M8

Species:

Leishmania braziliensis

UniProt:

A4HJI1_LEIBR

TriTrypDb:

LbrM.31.2240 , LBRM2903_310032100

Length:

297

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 41
NetGPI	no	yes: 0, no: 41

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	42
GO:0110165	cellular anatomical entity	1	42

Expansion

Sequence features

A4HJI1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJI1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	42
GO:0006807	nitrogen compound metabolic process	2	42
GO:0007155	cell adhesion	2	42
GO:0008152	metabolic process	1	42
GO:0009987	cellular process	1	42
GO:0019538	protein metabolic process	3	42
GO:0043170	macromolecule metabolic process	3	42
GO:0044238	primary metabolic process	2	42
GO:0071704	organic substance metabolic process	2	42
GO:1901564	organonitrogen compound metabolic process	3	42

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	42
GO:0004175	endopeptidase activity	4	42
GO:0004222	metalloendopeptidase activity	5	42
GO:0005488	binding	1	42
GO:0008233	peptidase activity	3	42
GO:0008237	metallopeptidase activity	4	42
GO:0016787	hydrolase activity	2	42
GO:0043167	ion binding	2	42
GO:0043169	cation binding	3	42
GO:0046872	metal ion binding	4	42
GO:0140096	catalytic activity, acting on a protein	2	42

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	21	23	PF00082	0.365
CLV_PCSK_KEX2_1	238	240	PF00082	0.509
CLV_PCSK_PC1ET2_1	21	23	PF00082	0.396
CLV_PCSK_PC1ET2_1	238	240	PF00082	0.341
CLV_PCSK_SKI1_1	70	74	PF00082	0.463
DEG_Nend_Nbox_1	1	3	PF02207	0.153
DOC_PP1_RVXF_1	52	58	PF00149	0.220
DOC_PP2B_LxvP_1	29	32	PF13499	0.349
DOC_PP4_FxxP_1	74	77	PF00568	0.354
DOC_USP7_MATH_1	119	123	PF00917	0.260
DOC_USP7_MATH_1	13	17	PF00917	0.281
DOC_USP7_MATH_1	228	232	PF00917	0.292
DOC_USP7_MATH_1	267	271	PF00917	0.547
DOC_WW_Pin1_4	180	185	PF00397	0.339
DOC_WW_Pin1_4	229	234	PF00397	0.277
DOC_WW_Pin1_4	83	88	PF00397	0.236
LIG_14-3-3_CanoR_1	280	288	PF00244	0.390
LIG_14-3-3_CanoR_1	38	43	PF00244	0.285
LIG_14-3-3_CanoR_1	99	108	PF00244	0.244
LIG_APCC_ABBAyCdc20_2	70	76	PF00400	0.141
LIG_FHA_1	201	207	PF00498	0.311
LIG_FHA_1	280	286	PF00498	0.653
LIG_FHA_1	84	90	PF00498	0.241
LIG_LIR_Apic_2	249	255	PF02991	0.333
LIG_LIR_Gen_1	201	211	PF02991	0.331
LIG_LIR_LC3C_4	178	181	PF02991	0.318
LIG_LIR_Nem_3	122	126	PF02991	0.301
LIG_LIR_Nem_3	140	145	PF02991	0.146
LIG_LIR_Nem_3	18	23	PF02991	0.292
LIG_LIR_Nem_3	201	207	PF02991	0.310
LIG_LIR_Nem_3	49	55	PF02991	0.193
LIG_RPA_C_Fungi	33	45	PF08784	0.153
LIG_SH2_CRK	142	146	PF00017	0.345
LIG_SH2_CRK	204	208	PF00017	0.457
LIG_SH2_CRK	252	256	PF00017	0.411
LIG_SH2_CRK	39	43	PF00017	0.276
LIG_SH2_GRB2like	252	255	PF00017	0.153
LIG_SH2_SRC	247	250	PF00017	0.347
LIG_SH2_SRC	252	255	PF00017	0.346
LIG_SH2_STAP1	116	120	PF00017	0.343
LIG_SH2_STAP1	123	127	PF00017	0.376
LIG_SH2_STAP1	204	208	PF00017	0.452
LIG_SH2_STAT5	126	129	PF00017	0.341
LIG_SH2_STAT5	142	145	PF00017	0.145
LIG_SH3_3	125	131	PF00018	0.348
LIG_SH3_3	142	148	PF00018	0.158
LIG_SH3_3	178	184	PF00018	0.455
LIG_SH3_3	227	233	PF00018	0.301
LIG_SUMO_SIM_anti_2	260	266	PF11976	0.649
MOD_CK1_1	183	189	PF00069	0.283
MOD_CK1_1	209	215	PF00069	0.342
MOD_CK1_1	40	46	PF00069	0.252
MOD_CK1_1	93	99	PF00069	0.240
MOD_CK2_1	55	61	PF00069	0.332
MOD_GlcNHglycan	117	120	PF01048	0.371
MOD_GlcNHglycan	208	211	PF01048	0.388
MOD_GlcNHglycan	282	285	PF01048	0.563
MOD_GlcNHglycan	96	99	PF01048	0.332
MOD_GSK3_1	111	118	PF00069	0.405
MOD_GSK3_1	129	136	PF00069	0.400
MOD_GSK3_1	209	216	PF00069	0.369
MOD_GSK3_1	263	270	PF00069	0.494
MOD_GSK3_1	77	84	PF00069	0.236
MOD_GSK3_1	90	97	PF00069	0.214
MOD_LATS_1	278	284	PF00433	0.397
MOD_N-GLC_1	198	203	PF02516	0.306
MOD_N-GLC_1	55	60	PF02516	0.262
MOD_NEK2_1	133	138	PF00069	0.399
MOD_NEK2_1	149	154	PF00069	0.201
MOD_NEK2_1	90	95	PF00069	0.280
MOD_PIKK_1	55	61	PF00454	0.409
MOD_PKA_1	238	244	PF00069	0.153
MOD_PKA_2	104	110	PF00069	0.253
MOD_PKA_2	238	244	PF00069	0.393
MOD_PKA_2	279	285	PF00069	0.686
MOD_PKA_2	37	43	PF00069	0.398
MOD_PKA_2	93	99	PF00069	0.294
MOD_Plk_4	209	215	PF00069	0.290
MOD_Plk_4	85	91	PF00069	0.396
MOD_ProDKin_1	180	186	PF00069	0.431
MOD_ProDKin_1	229	235	PF00069	0.344
MOD_ProDKin_1	83	89	PF00069	0.287
MOD_SUMO_for_1	25	28	PF00179	0.299
TRG_ENDOCYTIC_2	142	145	PF00928	0.399
TRG_ENDOCYTIC_2	204	207	PF00928	0.429
TRG_ENDOCYTIC_2	247	250	PF00928	0.314
TRG_ENDOCYTIC_2	39	42	PF00928	0.274

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NEJ8	Trypanosomatidae	28%	100%
A0A1X0NEL1	Trypanosomatidae	28%	70%
A0A1X0NES0	Trypanosomatidae	29%	98%
A0A1X0NGP1	Trypanosomatidae	38%	80%
A0A1X0NGS6	Trypanosomatidae	37%	67%
A0A1X0NHF4	Trypanosomatidae	29%	96%
A0A1X0NJC6	Trypanosomatidae	34%	70%
A0A1X0NKS8	Trypanosomatidae	37%	73%
A0A1X0NP29	Trypanosomatidae	35%	95%
A0A1X0NQ02	Trypanosomatidae	31%	95%
A0A1X0NQN3	Trypanosomatidae	30%	68%
A0A1X0NQU3	Trypanosomatidae	39%	78%
A0A1X0NR02	Trypanosomatidae	40%	87%
A0A1X0NWK7	Trypanosomatidae	36%	74%
A0A1X0NY94	Trypanosomatidae	38%	70%
A0A1X0NYQ7	Trypanosomatidae	28%	100%
A0A1X0NYX4	Trypanosomatidae	27%	96%
A0A1X0P047	Trypanosomatidae	28%	100%
A0A1X0P5K2	Trypanosomatidae	34%	67%
A0A3R7JUH9	Trypanosoma rangeli	39%	86%
A0A3R7KFR4	Trypanosoma rangeli	31%	93%
A0A3R7KJC7	Trypanosoma rangeli	39%	72%
A0A3R7LGK6	Trypanosoma rangeli	38%	100%
A0A3R7M2G4	Trypanosoma rangeli	31%	72%
A0A3R7RCC3	Trypanosoma rangeli	25%	100%
A0A422MQQ6	Trypanosoma rangeli	29%	78%
A0A422MUP1	Trypanosoma rangeli	39%	87%
A0A422MXE4	Trypanosoma rangeli	40%	100%
A0A422NWT8	Trypanosoma rangeli	26%	100%
A4H629	Leishmania braziliensis	37%	100%
A4H6D0	Leishmania braziliensis	34%	100%
A4H6E4	Leishmania braziliensis	37%	74%
D0A7W4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	67%
V5A303	Trypanosoma cruzi	40%	71%
V5AN34	Trypanosoma cruzi	44%	71%
V5ANT4	Trypanosoma cruzi	40%	92%
V5AQ95	Trypanosoma cruzi	31%	80%
V5BAB0	Trypanosoma cruzi	37%	84%
V5CIW6	Trypanosoma cruzi	30%	82%
V5CJ71	Trypanosoma cruzi	28%	80%