Putative dihydrouridine synthase (Dus)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dihydrouridine synthase (Dus), putative

Species:

Leishmania braziliensis

UniProt:

A4HJH5_LEIBR

TriTrypDb:

LbrM.31.2160 , LBRM2903_310031600 , LBRM2903_310031700

Length:

666

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

A4HJH5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJH5

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	10
GO:0016491	oxidoreductase activity	2	10
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	10
GO:0017150	tRNA dihydrouridine synthase activity	5	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0050660	flavin adenine dinucleotide binding	4	10
GO:0097159	organic cyclic compound binding	2	10
GO:0106413	dihydrouridine synthase activity	4	10
GO:0140098	catalytic activity, acting on RNA	3	10
GO:0140101	catalytic activity, acting on a tRNA	4	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	339	343	PF00656	0.404
CLV_C14_Caspase3-7	76	80	PF00656	0.590
CLV_NRD_NRD_1	295	297	PF00675	0.268
CLV_NRD_NRD_1	426	428	PF00675	0.265
CLV_NRD_NRD_1	58	60	PF00675	0.477
CLV_PCSK_KEX2_1	295	297	PF00082	0.268
CLV_PCSK_KEX2_1	426	428	PF00082	0.281
CLV_PCSK_SKI1_1	33	37	PF00082	0.547
CLV_PCSK_SKI1_1	410	414	PF00082	0.291
CLV_PCSK_SKI1_1	429	433	PF00082	0.291
CLV_PCSK_SKI1_1	6	10	PF00082	0.349
DEG_APCC_DBOX_1	537	545	PF00400	0.531
DEG_SCF_FBW7_2	161	168	PF00400	0.370
DEG_SCF_FBW7_2	568	575	PF00400	0.464
DOC_CYCLIN_yCln2_LP_2	321	327	PF00134	0.465
DOC_MAPK_gen_1	426	432	PF00069	0.432
DOC_MAPK_gen_1	552	562	PF00069	0.521
DOC_MAPK_gen_1	647	655	PF00069	0.553
DOC_MAPK_MEF2A_6	510	518	PF00069	0.450
DOC_PP2B_LxvP_1	321	324	PF13499	0.465
DOC_PP4_FxxP_1	177	180	PF00568	0.449
DOC_PP4_FxxP_1	332	335	PF00568	0.531
DOC_PP4_FxxP_1	475	478	PF00568	0.465
DOC_USP7_MATH_1	155	159	PF00917	0.563
DOC_USP7_MATH_1	178	182	PF00917	0.612
DOC_USP7_MATH_1	20	24	PF00917	0.453
DOC_USP7_UBL2_3	552	556	PF12436	0.467
DOC_USP7_UBL2_3	645	649	PF12436	0.613
DOC_WW_Pin1_4	108	113	PF00397	0.382
DOC_WW_Pin1_4	139	144	PF00397	0.448
DOC_WW_Pin1_4	161	166	PF00397	0.314
DOC_WW_Pin1_4	176	181	PF00397	0.620
DOC_WW_Pin1_4	349	354	PF00397	0.479
DOC_WW_Pin1_4	568	573	PF00397	0.641
DOC_WW_Pin1_4	603	608	PF00397	0.440
DOC_WW_Pin1_4	63	68	PF00397	0.543
DOC_WW_Pin1_4	83	88	PF00397	0.553
LIG_14-3-3_CanoR_1	22	27	PF00244	0.474
LIG_14-3-3_CanoR_1	251	256	PF00244	0.572
LIG_14-3-3_CanoR_1	495	503	PF00244	0.531
LIG_14-3-3_CanoR_1	630	638	PF00244	0.554
LIG_APCC_ABBA_1	582	587	PF00400	0.480
LIG_BIR_II_1	1	5	PF00653	0.488
LIG_FHA_1	140	146	PF00498	0.531
LIG_FHA_1	252	258	PF00498	0.524
LIG_FHA_1	314	320	PF00498	0.548
LIG_FHA_1	324	330	PF00498	0.558
LIG_FHA_1	367	373	PF00498	0.471
LIG_FHA_1	433	439	PF00498	0.561
LIG_FHA_1	499	505	PF00498	0.566
LIG_FHA_1	557	563	PF00498	0.705
LIG_FHA_1	572	578	PF00498	0.505
LIG_FHA_2	197	203	PF00498	0.560
LIG_FHA_2	444	450	PF00498	0.459
LIG_FHA_2	455	461	PF00498	0.468
LIG_GBD_Chelix_1	313	321	PF00786	0.361
LIG_GBD_Chelix_1	613	621	PF00786	0.248
LIG_LIR_Apic_2	174	180	PF02991	0.421
LIG_LIR_Gen_1	158	166	PF02991	0.580
LIG_LIR_Gen_1	305	314	PF02991	0.448
LIG_LIR_Gen_1	388	398	PF02991	0.500
LIG_LIR_Gen_1	446	454	PF02991	0.448
LIG_LIR_Gen_1	51	61	PF02991	0.354
LIG_LIR_Gen_1	555	563	PF02991	0.455
LIG_LIR_Gen_1	608	617	PF02991	0.347
LIG_LIR_Nem_3	125	131	PF02991	0.448
LIG_LIR_Nem_3	158	163	PF02991	0.503
LIG_LIR_Nem_3	305	310	PF02991	0.448
LIG_LIR_Nem_3	446	450	PF02991	0.448
LIG_LIR_Nem_3	51	56	PF02991	0.328
LIG_LIR_Nem_3	608	613	PF02991	0.316
LIG_PDZ_Class_2	661	666	PF00595	0.651
LIG_Pex14_1	508	512	PF04695	0.531
LIG_Pex14_2	524	528	PF04695	0.491
LIG_SH2_CRK	11	15	PF00017	0.408
LIG_SH2_CRK	61	65	PF00017	0.489
LIG_SH2_NCK_1	61	65	PF00017	0.450
LIG_SH2_SRC	447	450	PF00017	0.531
LIG_SH2_STAP1	391	395	PF00017	0.448
LIG_SH2_STAP1	447	451	PF00017	0.531
LIG_SH2_STAT5	141	144	PF00017	0.465
LIG_SH2_STAT5	28	31	PF00017	0.453
LIG_SH3_2	481	486	PF14604	0.531
LIG_SH3_3	113	119	PF00018	0.251
LIG_SH3_3	160	166	PF00018	0.572
LIG_SH3_3	208	214	PF00018	0.483
LIG_SH3_3	253	259	PF00018	0.583
LIG_SH3_3	475	481	PF00018	0.465
LIG_SH3_3	601	607	PF00018	0.386
LIG_SH3_3	61	67	PF00018	0.550
LIG_SUMO_SIM_anti_2	207	212	PF11976	0.297
LIG_SUMO_SIM_anti_2	384	391	PF11976	0.453
LIG_SUMO_SIM_par_1	651	658	PF11976	0.488
LIG_TRAF2_1	268	271	PF00917	0.560
LIG_TRAF2_1	378	381	PF00917	0.357
LIG_TRAF2_1	648	651	PF00917	0.594
LIG_WRC_WIRS_1	299	304	PF05994	0.465
MOD_CDK_SPK_2	108	113	PF00069	0.424
MOD_CDK_SPK_2	568	573	PF00069	0.469
MOD_CDK_SPK_2	83	88	PF00069	0.519
MOD_CDK_SPxxK_3	63	70	PF00069	0.463
MOD_CK1_1	188	194	PF00069	0.686
MOD_CK1_1	309	315	PF00069	0.383
MOD_CK1_1	476	482	PF00069	0.471
MOD_CK1_1	66	72	PF00069	0.319
MOD_CK2_1	146	152	PF00069	0.400
MOD_CK2_1	180	186	PF00069	0.529
MOD_CK2_1	264	270	PF00069	0.698
MOD_CK2_1	454	460	PF00069	0.491
MOD_CK2_1	605	611	PF00069	0.316
MOD_CK2_1	92	98	PF00069	0.582
MOD_CMANNOS	50	53	PF00535	0.394
MOD_GlcNHglycan	182	185	PF01048	0.555
MOD_GlcNHglycan	186	190	PF01048	0.551
MOD_GlcNHglycan	432	435	PF01048	0.183
MOD_GlcNHglycan	47	50	PF01048	0.580
MOD_GlcNHglycan	488	491	PF01048	0.325
MOD_GlcNHglycan	496	499	PF01048	0.327
MOD_GlcNHglycan	589	595	PF01048	0.597
MOD_GlcNHglycan	607	610	PF01048	0.555
MOD_GlcNHglycan	639	642	PF01048	0.729
MOD_GlcNHglycan	88	91	PF01048	0.671
MOD_GSK3_1	135	142	PF00069	0.462
MOD_GSK3_1	155	162	PF00069	0.551
MOD_GSK3_1	176	183	PF00069	0.587
MOD_GSK3_1	188	195	PF00069	0.705
MOD_GSK3_1	309	316	PF00069	0.531
MOD_GSK3_1	40	47	PF00069	0.583
MOD_GSK3_1	494	501	PF00069	0.543
MOD_GSK3_1	571	578	PF00069	0.697
MOD_GSK3_1	73	80	PF00069	0.654
MOD_GSK3_1	88	95	PF00069	0.585
MOD_N-GLC_1	264	269	PF02516	0.471
MOD_NEK2_1	21	26	PF00069	0.486
MOD_NEK2_1	430	435	PF00069	0.467
MOD_NEK2_1	454	459	PF00069	0.448
MOD_NEK2_1	544	549	PF00069	0.555
MOD_NEK2_1	73	78	PF00069	0.638
MOD_PIKK_1	476	482	PF00454	0.561
MOD_PKA_2	21	27	PF00069	0.482
MOD_PKA_2	250	256	PF00069	0.689
MOD_PKA_2	494	500	PF00069	0.495
MOD_PKA_2	629	635	PF00069	0.537
MOD_Plk_1	33	39	PF00069	0.583
MOD_Plk_1	575	581	PF00069	0.551
MOD_Plk_1	73	79	PF00069	0.598
MOD_Plk_4	298	304	PF00069	0.465
MOD_Plk_4	309	315	PF00069	0.531
MOD_Plk_4	385	391	PF00069	0.401
MOD_ProDKin_1	108	114	PF00069	0.368
MOD_ProDKin_1	139	145	PF00069	0.448
MOD_ProDKin_1	161	167	PF00069	0.322
MOD_ProDKin_1	176	182	PF00069	0.626
MOD_ProDKin_1	349	355	PF00069	0.478
MOD_ProDKin_1	568	574	PF00069	0.638
MOD_ProDKin_1	603	609	PF00069	0.427
MOD_ProDKin_1	63	69	PF00069	0.539
MOD_ProDKin_1	83	89	PF00069	0.552
MOD_SUMO_for_1	648	651	PF00179	0.594
MOD_SUMO_rev_2	162	172	PF00179	0.476
MOD_SUMO_rev_2	466	475	PF00179	0.531
TRG_DiLeu_BaEn_4	539	545	PF01217	0.503
TRG_DiLeu_BaEn_4	575	581	PF01217	0.643
TRG_DiLeu_BaEn_4	657	663	PF01217	0.514
TRG_DiLeu_BaLyEn_6	464	469	PF01217	0.465
TRG_ENDOCYTIC_2	11	14	PF00928	0.408
TRG_ENDOCYTIC_2	141	144	PF00928	0.448
TRG_ENDOCYTIC_2	299	302	PF00928	0.465
TRG_ENDOCYTIC_2	391	394	PF00928	0.448
TRG_ENDOCYTIC_2	447	450	PF00928	0.531
TRG_ENDOCYTIC_2	61	64	PF00928	0.502
TRG_ER_diArg_1	425	427	PF00400	0.465
TRG_NES_CRM1_1	308	322	PF08389	0.463
TRG_Pf-PMV_PEXEL_1	12	17	PF00026	0.515
TRG_Pf-PMV_PEXEL_1	129	134	PF00026	0.331
TRG_Pf-PMV_PEXEL_1	377	381	PF00026	0.365
TRG_Pf-PMV_PEXEL_1	393	397	PF00026	0.331
TRG_Pf-PMV_PEXEL_1	622	626	PF00026	0.337

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HS31	Leptomonas seymouri	58%	100%
A0A0S4JUA9	Bodo saltans	37%	100%
A0A3Q8ICM1	Leishmania donovani	79%	100%
A4I6W8	Leishmania infantum	79%	100%
C9ZWQ2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9B1Z2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
Q4Q669	Leishmania major	77%	99%
V5D2B4	Trypanosoma cruzi	39%	100%