Putative dihydroceramide synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Sphingosine N-acyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4HJG0_LEIBR

TriTrypDb:

LbrM.31.2010 , LBRM2903_310029600

Length:

459

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HJG0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJG0

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	13
GO:0006643	membrane lipid metabolic process	4	13
GO:0006665	sphingolipid metabolic process	4	13
GO:0006672	ceramide metabolic process	4	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0008610	lipid biosynthetic process	4	13
GO:0009058	biosynthetic process	2	13
GO:0009987	cellular process	1	13
GO:0030148	sphingolipid biosynthetic process	5	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043603	amide metabolic process	3	13
GO:0043604	amide biosynthetic process	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044249	cellular biosynthetic process	3	13
GO:0044255	cellular lipid metabolic process	3	13
GO:0044271	cellular nitrogen compound biosynthetic process	4	13
GO:0046467	membrane lipid biosynthetic process	4	13
GO:0046513	ceramide biosynthetic process	5	13
GO:0071704	organic substance metabolic process	2	13
GO:1901564	organonitrogen compound metabolic process	3	13
GO:1901566	organonitrogen compound biosynthetic process	4	13
GO:1901576	organic substance biosynthetic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0016410	N-acyltransferase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016746	acyltransferase activity	3	13
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	13
GO:0050291	sphingosine N-acyltransferase activity	6	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	246	252	PF00089	0.513
CLV_NRD_NRD_1	121	123	PF00675	0.270
CLV_NRD_NRD_1	127	129	PF00675	0.312
CLV_NRD_NRD_1	35	37	PF00675	0.630
CLV_NRD_NRD_1	386	388	PF00675	0.320
CLV_NRD_NRD_1	452	454	PF00675	0.537
CLV_PCSK_KEX2_1	120	122	PF00082	0.283
CLV_PCSK_KEX2_1	127	129	PF00082	0.320
CLV_PCSK_KEX2_1	221	223	PF00082	0.202
CLV_PCSK_KEX2_1	287	289	PF00082	0.435
CLV_PCSK_KEX2_1	35	37	PF00082	0.630
CLV_PCSK_KEX2_1	386	388	PF00082	0.320
CLV_PCSK_KEX2_1	424	426	PF00082	0.373
CLV_PCSK_KEX2_1	452	454	PF00082	0.539
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.222
CLV_PCSK_PC1ET2_1	287	289	PF00082	0.396
CLV_PCSK_PC1ET2_1	386	388	PF00082	0.360
CLV_PCSK_PC1ET2_1	424	426	PF00082	0.373
CLV_PCSK_SKI1_1	140	144	PF00082	0.342
CLV_PCSK_SKI1_1	218	222	PF00082	0.291
CLV_PCSK_SKI1_1	300	304	PF00082	0.453
CLV_PCSK_SKI1_1	341	345	PF00082	0.166
CLV_PCSK_SKI1_1	38	42	PF00082	0.561
DEG_SPOP_SBC_1	436	440	PF00917	0.557
DOC_CKS1_1	442	447	PF01111	0.551
DOC_CYCLIN_RxL_1	92	103	PF00134	0.208
DOC_CYCLIN_yCln2_LP_2	185	191	PF00134	0.313
DOC_CYCLIN_yCln2_LP_2	77	83	PF00134	0.250
DOC_MAPK_gen_1	136	144	PF00069	0.464
DOC_MAPK_MEF2A_6	143	152	PF00069	0.436
DOC_PP1_RVXF_1	138	145	PF00149	0.471
DOC_PP1_RVXF_1	93	100	PF00149	0.218
DOC_PP2B_LxvP_1	25	28	PF13499	0.400
DOC_PP4_FxxP_1	171	174	PF00568	0.211
DOC_PP4_FxxP_1	442	445	PF00568	0.548
DOC_SPAK_OSR1_1	104	108	PF12202	0.503
DOC_USP7_MATH_1	436	440	PF00917	0.631
DOC_USP7_UBL2_3	139	143	PF12436	0.495
DOC_USP7_UBL2_3	292	296	PF12436	0.320
DOC_USP7_UBL2_3	424	428	PF12436	0.753
DOC_WW_Pin1_4	131	136	PF00397	0.509
DOC_WW_Pin1_4	14	19	PF00397	0.433
DOC_WW_Pin1_4	427	432	PF00397	0.559
DOC_WW_Pin1_4	441	446	PF00397	0.527
LIG_14-3-3_CanoR_1	128	134	PF00244	0.626
LIG_14-3-3_CanoR_1	305	315	PF00244	0.301
LIG_14-3-3_CanoR_1	46	54	PF00244	0.359
LIG_Actin_WH2_2	33	48	PF00022	0.296
LIG_BIR_II_1	1	5	PF00653	0.426
LIG_BRCT_BRCA1_1	156	160	PF00533	0.237
LIG_BRCT_BRCA1_1	311	315	PF00533	0.159
LIG_BRCT_BRCA1_1	321	325	PF00533	0.283
LIG_BRCT_BRCA1_1	438	442	PF00533	0.678
LIG_Clathr_ClatBox_1	213	217	PF01394	0.399
LIG_EH1_1	231	239	PF00400	0.309
LIG_FHA_1	233	239	PF00498	0.247
LIG_FHA_1	258	264	PF00498	0.261
LIG_FHA_2	408	414	PF00498	0.626
LIG_GBD_Chelix_1	355	363	PF00786	0.284
LIG_GBD_Chelix_1	89	97	PF00786	0.159
LIG_LIR_Apic_2	16	22	PF02991	0.398
LIG_LIR_Apic_2	439	445	PF02991	0.550
LIG_LIR_Gen_1	100	109	PF02991	0.542
LIG_LIR_Gen_1	312	323	PF02991	0.192
LIG_LIR_Gen_1	44	55	PF02991	0.335
LIG_LIR_Nem_3	100	105	PF02991	0.575
LIG_LIR_Nem_3	151	156	PF02991	0.264
LIG_LIR_Nem_3	157	163	PF02991	0.295
LIG_LIR_Nem_3	167	173	PF02991	0.260
LIG_LIR_Nem_3	197	201	PF02991	0.286
LIG_LIR_Nem_3	223	228	PF02991	0.453
LIG_LIR_Nem_3	247	253	PF02991	0.233
LIG_LIR_Nem_3	301	307	PF02991	0.160
LIG_LIR_Nem_3	312	318	PF02991	0.221
LIG_LIR_Nem_3	322	327	PF02991	0.205
LIG_LIR_Nem_3	34	40	PF02991	0.368
LIG_LIR_Nem_3	44	50	PF02991	0.308
LIG_MLH1_MIPbox_1	321	325	PF16413	0.283
LIG_MYND_1	79	83	PF01753	0.178
LIG_NRBOX	354	360	PF00104	0.237
LIG_Pex14_1	149	153	PF04695	0.302
LIG_Pex14_1	353	357	PF04695	0.343
LIG_Pex14_2	320	324	PF04695	0.237
LIG_REV1ctd_RIR_1	322	332	PF16727	0.253
LIG_RPA_C_Fungi	116	128	PF08784	0.399
LIG_SH2_CRK	108	112	PF00017	0.399
LIG_SH2_CRK	189	193	PF00017	0.348
LIG_SH2_CRK	19	23	PF00017	0.500
LIG_SH2_CRK	304	308	PF00017	0.167
LIG_SH2_CRK	330	334	PF00017	0.302
LIG_SH2_NCK_1	189	193	PF00017	0.391
LIG_SH2_NCK_1	19	23	PF00017	0.470
LIG_SH2_STAP1	206	210	PF00017	0.159
LIG_SH2_STAT3	162	165	PF00017	0.249
LIG_SH2_STAT3	273	276	PF00017	0.391
LIG_SH2_STAT5	154	157	PF00017	0.274
LIG_SH2_STAT5	163	166	PF00017	0.299
LIG_SH2_STAT5	228	231	PF00017	0.180
LIG_SH2_STAT5	253	256	PF00017	0.285
LIG_SH2_STAT5	26	29	PF00017	0.625
LIG_SH2_STAT5	306	309	PF00017	0.314
LIG_SH2_STAT5	330	333	PF00017	0.360
LIG_SH2_STAT5	96	99	PF00017	0.398
LIG_SH3_3	185	191	PF00018	0.277
LIG_SH3_3	395	401	PF00018	0.562
LIG_SUMO_SIM_anti_2	235	240	PF11976	0.246
LIG_SUMO_SIM_anti_2	371	376	PF11976	0.159
LIG_SUMO_SIM_anti_2	74	79	PF11976	0.308
LIG_SUMO_SIM_par_1	259	268	PF11976	0.259
LIG_TRAF2_1	191	194	PF00917	0.369
LIG_TRAF2_1	280	283	PF00917	0.273
LIG_TYR_ITIM	302	307	PF00017	0.279
LIG_UBA3_1	213	221	PF00899	0.302
LIG_UBA3_1	374	383	PF00899	0.159
MOD_CDK_SPK_2	131	136	PF00069	0.386
MOD_CDK_SPxK_1	441	447	PF00069	0.445
MOD_CK1_1	438	444	PF00069	0.592
MOD_CK1_1	6	12	PF00069	0.546
MOD_CK2_1	18	24	PF00069	0.509
MOD_CK2_1	389	395	PF00069	0.540
MOD_Cter_Amidation	125	128	PF01082	0.491
MOD_Cter_Amidation	384	387	PF01082	0.382
MOD_GlcNHglycan	114	118	PF01048	0.381
MOD_GlcNHglycan	206	209	PF01048	0.416
MOD_GlcNHglycan	293	296	PF01048	0.446
MOD_GlcNHglycan	68	71	PF01048	0.424
MOD_GSK3_1	14	21	PF00069	0.551
MOD_GSK3_1	263	270	PF00069	0.347
MOD_GSK3_1	272	279	PF00069	0.341
MOD_GSK3_1	332	339	PF00069	0.309
MOD_GSK3_1	409	416	PF00069	0.576
MOD_GSK3_1	41	48	PF00069	0.501
MOD_GSK3_1	436	443	PF00069	0.709
MOD_N-GLC_1	31	36	PF02516	0.533
MOD_N-GLC_1	447	452	PF02516	0.586
MOD_N-GLC_1	453	458	PF02516	0.548
MOD_N-GLC_2	314	316	PF02516	0.343
MOD_NEK2_1	148	153	PF00069	0.362
MOD_NEK2_1	155	160	PF00069	0.350
MOD_NEK2_1	265	270	PF00069	0.249
MOD_NEK2_1	30	35	PF00069	0.398
MOD_NEK2_1	377	382	PF00069	0.351
MOD_NEK2_1	437	442	PF00069	0.693
MOD_NEK2_2	298	303	PF00069	0.159
MOD_PIKK_1	272	278	PF00454	0.279
MOD_PKA_1	452	458	PF00069	0.607
MOD_PKA_2	399	405	PF00069	0.510
MOD_PKA_2	45	51	PF00069	0.510
MOD_PKA_2	452	458	PF00069	0.607
MOD_PKB_1	36	44	PF00069	0.379
MOD_Plk_1	177	183	PF00069	0.411
MOD_Plk_1	309	315	PF00069	0.235
MOD_Plk_1	41	47	PF00069	0.341
MOD_Plk_1	73	79	PF00069	0.287
MOD_Plk_2-3	389	395	PF00069	0.471
MOD_Plk_4	148	154	PF00069	0.282
MOD_Plk_4	194	200	PF00069	0.429
MOD_Plk_4	209	215	PF00069	0.328
MOD_Plk_4	298	304	PF00069	0.346
MOD_Plk_4	319	325	PF00069	0.283
MOD_Plk_4	377	383	PF00069	0.337
MOD_Plk_4	73	79	PF00069	0.272
MOD_ProDKin_1	131	137	PF00069	0.382
MOD_ProDKin_1	14	20	PF00069	0.561
MOD_ProDKin_1	427	433	PF00069	0.456
MOD_ProDKin_1	441	447	PF00069	0.414
TRG_ENDOCYTIC_2	108	111	PF00928	0.323
TRG_ENDOCYTIC_2	253	256	PF00928	0.285
TRG_ENDOCYTIC_2	304	307	PF00928	0.167
TRG_ENDOCYTIC_2	369	372	PF00928	0.360
TRG_ENDOCYTIC_2	86	89	PF00928	0.361
TRG_ENDOCYTIC_2	96	99	PF00928	0.310
TRG_ER_diArg_1	120	122	PF00400	0.401
TRG_ER_diArg_1	35	37	PF00400	0.414
TRG_ER_diArg_1	451	453	PF00400	0.596
TRG_Pf-PMV_PEXEL_1	95	100	PF00026	0.458

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2N8	Leptomonas seymouri	64%	100%
A0A0S4JIF9	Bodo saltans	35%	100%
A0A1X0NPU5	Trypanosomatidae	44%	100%
A0A1X0NPY1	Trypanosomatidae	46%	100%
A0A3S7X4J5	Leishmania donovani	70%	100%
A0A422MNN3	Trypanosoma rangeli	46%	100%
A4I6V5	Leishmania infantum	70%	100%
C9ZMC0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
C9ZWR6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9B1X8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
G5ED45	Caenorhabditis elegans	26%	100%
Q4Q684	Leishmania major	69%	100%
Q9D6J1	Mus musculus	31%	100%
Q9LDF2	Arabidopsis thaliana	26%	100%