GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania braziliensis

UniProt:

A4HJ91_LEIBR

TriTrypDb:

LbrM.31.1310 , LBRM2903_310021200

Length:

215

Annotations

LeishMANIAdb annotations

Related to other eukaryotic COPII adaptors. Likely involved in ER to Golgi transport.. A Leishmaniid-specific subgroup of these proteins is missing the SigP (unclear topology and function). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 12, no: 7
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	20
GO:0110165	cellular anatomical entity	1	20
GO:0005783	endoplasmic reticulum	5	2
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	2
GO:0005794	Golgi apparatus	5	2
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	2
GO:0030135	coated vesicle	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2

Expansion

Sequence features

A4HJ91

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HJ91

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0006996	organelle organization	4	2
GO:0007030	Golgi organization	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016043	cellular component organization	3	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071840	cellular component organization or biogenesis	2	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	11	13	PF00675	0.507
CLV_NRD_NRD_1	56	58	PF00675	0.351
CLV_PCSK_KEX2_1	11	13	PF00082	0.650
CLV_PCSK_KEX2_1	206	208	PF00082	0.237
CLV_PCSK_KEX2_1	56	58	PF00082	0.365
CLV_PCSK_PC1ET2_1	206	208	PF00082	0.199
CLV_PCSK_SKI1_1	181	185	PF00082	0.392
CLV_PCSK_SKI1_1	2	6	PF00082	0.534
CLV_PCSK_SKI1_1	206	210	PF00082	0.197
CLV_Separin_Metazoa	83	87	PF03568	0.151
DOC_CYCLIN_RxL_1	203	213	PF00134	0.261
DOC_MAPK_RevD_3	198	212	PF00069	0.315
DOC_PP1_RVXF_1	102	109	PF00149	0.263
DOC_PP1_RVXF_1	118	125	PF00149	0.219
DOC_PP1_RVXF_1	179	186	PF00149	0.236
DOC_PP1_RVXF_1	55	62	PF00149	0.274
DOC_PP4_FxxP_1	95	98	PF00568	0.324
DOC_USP7_MATH_1	135	139	PF00917	0.187
DOC_USP7_MATH_1	31	35	PF00917	0.546
DOC_WW_Pin1_4	29	34	PF00397	0.334
LIG_14-3-3_CanoR_1	127	131	PF00244	0.333
LIG_14-3-3_CanoR_1	173	177	PF00244	0.198
LIG_14-3-3_CanoR_1	2	10	PF00244	0.546
LIG_APCC_ABBA_1	63	68	PF00400	0.291
LIG_BIR_III_2	15	19	PF00653	0.313
LIG_eIF4E_1	203	209	PF01652	0.159
LIG_FHA_1	101	107	PF00498	0.302
LIG_FHA_1	158	164	PF00498	0.231
LIG_FHA_1	3	9	PF00498	0.533
LIG_FHA_1	48	54	PF00498	0.258
LIG_FHA_2	78	84	PF00498	0.291
LIG_GBD_Chelix_1	192	200	PF00786	0.159
LIG_GBD_Chelix_1	21	29	PF00786	0.564
LIG_IRF3_LxIS_1	158	164	PF10401	0.240
LIG_LIR_Apic_2	68	72	PF02991	0.318
LIG_LIR_Gen_1	187	197	PF02991	0.323
LIG_LIR_Nem_3	15	20	PF02991	0.402
LIG_LIR_Nem_3	187	192	PF02991	0.312
LIG_NRBOX	204	210	PF00104	0.284
LIG_Pex14_1	185	189	PF04695	0.222
LIG_SH2_STAT5	203	206	PF00017	0.199
LIG_SH2_STAT5	69	72	PF00017	0.371
LIG_Sin3_3	17	24	PF02671	0.305
LIG_SUMO_SIM_par_1	194	199	PF11976	0.355
LIG_SUMO_SIM_par_1	71	77	PF11976	0.257
LIG_TRAF2_1	80	83	PF00917	0.313
LIG_TRFH_1	63	67	PF08558	0.286
LIG_UBA3_1	204	212	PF00899	0.316
LIG_WRC_WIRS_1	107	112	PF05994	0.332
MOD_CK1_1	100	106	PF00069	0.246
MOD_CK2_1	77	83	PF00069	0.323
MOD_GlcNHglycan	151	154	PF01048	0.513
MOD_GlcNHglycan	33	36	PF01048	0.661
MOD_GSK3_1	106	113	PF00069	0.321
MOD_GSK3_1	157	164	PF00069	0.296
MOD_GSK3_1	93	100	PF00069	0.306
MOD_NEK2_1	110	115	PF00069	0.266
MOD_NEK2_1	116	121	PF00069	0.271
MOD_NEK2_1	126	131	PF00069	0.269
MOD_NEK2_1	151	156	PF00069	0.309
MOD_NEK2_1	161	166	PF00069	0.240
MOD_NEK2_1	184	189	PF00069	0.256
MOD_PKA_2	126	132	PF00069	0.331
MOD_PKA_2	172	178	PF00069	0.226
MOD_PKA_2	77	83	PF00069	0.273
MOD_Plk_1	100	106	PF00069	0.197
MOD_Plk_4	112	118	PF00069	0.289
MOD_Plk_4	4	10	PF00069	0.516
MOD_ProDKin_1	29	35	PF00069	0.338
MOD_SUMO_rev_2	139	148	PF00179	0.284
TRG_DiLeu_BaLyEn_6	15	20	PF01217	0.498
TRG_DiLeu_BaLyEn_6	204	209	PF01217	0.261
TRG_ER_diArg_1	10	12	PF00400	0.371
TRG_ER_diLys_1	211	215	PF00400	0.658
TRG_Pf-PMV_PEXEL_1	206	210	PF00026	0.223
TRG_Pf-PMV_PEXEL_1	57	62	PF00026	0.444

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2E2	Leptomonas seymouri	58%	100%
A0A0N1II77	Leptomonas seymouri	42%	100%
A0A0N1PA81	Leptomonas seymouri	41%	100%
A0A0S4JG68	Bodo saltans	30%	97%
A0A0S4JHA6	Bodo saltans	28%	100%
A0A1X0NKZ3	Trypanosomatidae	30%	97%
A0A3R7KJS3	Trypanosoma rangeli	33%	99%
A0A3S7X4D1	Leishmania donovani	41%	66%
A0A3S7X4G7	Leishmania donovani	73%	100%
A4HJ92	Leishmania braziliensis	42%	100%
A4I6N5	Leishmania infantum	73%	100%
A4I6N6	Leishmania infantum	41%	100%
C9ZWV3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
E9B1Q8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	71%	100%
E9B1Q9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
O17528	Caenorhabditis elegans	23%	100%
Q4Q6F1	Leishmania major	43%	100%
Q4Q6F2	Leishmania major	68%	100%
Q9FVU0	Arabidopsis thaliana	25%	100%
V5BK13	Trypanosoma cruzi	33%	97%