Bifunctional lysine-specific demethylase and histidyl-hydroxylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cupin superfamily protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HIZ9_LEIBR

TriTrypDb:

LbrM.31.0350 , LBRM2903_310010200

Length:

595

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	8
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	8
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	8
GO:0110165	cellular anatomical entity	1	9
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

A4HIZ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIZ9

Function

Biological processes
Term	Name	Level	Count
GO:0006482	protein demethylation	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	2
GO:0008214	protein dealkylation	5	1
GO:0016570	histone modification	5	1
GO:0016577	histone demethylation	4	1
GO:0019538	protein metabolic process	3	1
GO:0034720	histone H3-K4 demethylation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070076	histone lysine demethylation	5	1
GO:0070544	histone H3-K36 demethylation	6	1
GO:0070988	demethylation	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:0032259	methylation	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005506	iron ion binding	6	9
GO:0016491	oxidoreductase activity	2	9
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	9
GO:0016706	2-oxoglutarate-dependent dioxygenase activity	4	9
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046914	transition metal ion binding	5	9
GO:0051213	dioxygenase activity	3	9
GO:0032451	demethylase activity	2	1
GO:0032452	histone demethylase activity	4	1
GO:0032453	histone H3K4 demethylase activity	5	1
GO:0051864	histone H3K36 demethylase activity	5	1
GO:0140096	catalytic activity, acting on a protein	2	1
GO:0140457	protein demethylase activity	3	1
GO:0008168	methyltransferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016741	transferase activity, transferring one-carbon groups	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	576	580	PF00656	0.396
CLV_MEL_PAP_1	211	217	PF00089	0.439
CLV_NRD_NRD_1	124	126	PF00675	0.581
CLV_NRD_NRD_1	150	152	PF00675	0.398
CLV_NRD_NRD_1	35	37	PF00675	0.480
CLV_NRD_NRD_1	428	430	PF00675	0.335
CLV_NRD_NRD_1	465	467	PF00675	0.376
CLV_PCSK_KEX2_1	101	103	PF00082	0.671
CLV_PCSK_KEX2_1	185	187	PF00082	0.406
CLV_PCSK_KEX2_1	213	215	PF00082	0.341
CLV_PCSK_KEX2_1	34	36	PF00082	0.489
CLV_PCSK_KEX2_1	428	430	PF00082	0.311
CLV_PCSK_KEX2_1	7	9	PF00082	0.423
CLV_PCSK_PC1ET2_1	101	103	PF00082	0.679
CLV_PCSK_PC1ET2_1	185	187	PF00082	0.300
CLV_PCSK_PC1ET2_1	213	215	PF00082	0.341
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.402
CLV_PCSK_SKI1_1	110	114	PF00082	0.593
CLV_PCSK_SKI1_1	185	189	PF00082	0.447
CLV_PCSK_SKI1_1	230	234	PF00082	0.268
CLV_PCSK_SKI1_1	393	397	PF00082	0.417
CLV_PCSK_SKI1_1	548	552	PF00082	0.299
DEG_SCF_FBW7_1	324	330	PF00400	0.297
DEG_SPOP_SBC_1	478	482	PF00917	0.339
DOC_CKS1_1	270	275	PF01111	0.260
DOC_CKS1_1	324	329	PF01111	0.325
DOC_MAPK_gen_1	230	240	PF00069	0.272
DOC_MAPK_HePTP_8	493	505	PF00069	0.407
DOC_MAPK_MEF2A_6	354	362	PF00069	0.311
DOC_MAPK_MEF2A_6	496	505	PF00069	0.397
DOC_MAPK_NFAT4_5	496	504	PF00069	0.383
DOC_PP1_RVXF_1	131	138	PF00149	0.320
DOC_PP1_RVXF_1	144	150	PF00149	0.206
DOC_PP1_RVXF_1	387	393	PF00149	0.382
DOC_PP2B_LxvP_1	21	24	PF13499	0.509
DOC_PP2B_LxvP_1	339	342	PF13499	0.284
DOC_PP2B_LxvP_1	503	506	PF13499	0.440
DOC_PP4_FxxP_1	240	243	PF00568	0.293
DOC_PP4_FxxP_1	560	563	PF00568	0.318
DOC_PP4_MxPP_1	468	471	PF00568	0.309
DOC_USP7_MATH_1	15	19	PF00917	0.483
DOC_USP7_MATH_1	24	28	PF00917	0.532
DOC_USP7_MATH_1	273	277	PF00917	0.260
DOC_USP7_MATH_1	478	482	PF00917	0.403
DOC_USP7_UBL2_3	110	114	PF12436	0.569
DOC_USP7_UBL2_3	152	156	PF12436	0.249
DOC_USP7_UBL2_3	206	210	PF12436	0.412
DOC_USP7_UBL2_3	350	354	PF12436	0.325
DOC_WW_Pin1_4	269	274	PF00397	0.260
DOC_WW_Pin1_4	323	328	PF00397	0.325
DOC_WW_Pin1_4	78	83	PF00397	0.515
LIG_14-3-3_CanoR_1	125	134	PF00244	0.423
LIG_14-3-3_CanoR_1	214	218	PF00244	0.432
LIG_14-3-3_CanoR_1	313	321	PF00244	0.271
LIG_14-3-3_CanoR_1	413	421	PF00244	0.394
LIG_14-3-3_CanoR_1	428	433	PF00244	0.435
LIG_14-3-3_CanoR_1	516	522	PF00244	0.343
LIG_14-3-3_CanoR_1	72	82	PF00244	0.475
LIG_APCC_ABBA_1	279	284	PF00400	0.260
LIG_BRCT_BRCA1_1	236	240	PF00533	0.288
LIG_BRCT_BRCA1_1	275	279	PF00533	0.260
LIG_Clathr_ClatBox_1	290	294	PF01394	0.234
LIG_Clathr_ClatBox_1	571	575	PF01394	0.413
LIG_CtBP_PxDLS_1	470	474	PF00389	0.300
LIG_eIF4E_1	217	223	PF01652	0.353
LIG_eIF4E_1	546	552	PF01652	0.252
LIG_FHA_1	11	17	PF00498	0.435
LIG_FHA_1	142	148	PF00498	0.312
LIG_FHA_1	217	223	PF00498	0.445
LIG_FHA_1	328	334	PF00498	0.387
LIG_FHA_1	353	359	PF00498	0.343
LIG_FHA_1	413	419	PF00498	0.530
LIG_FHA_1	442	448	PF00498	0.455
LIG_FHA_1	547	553	PF00498	0.398
LIG_FHA_2	449	455	PF00498	0.356
LIG_FHA_2	481	487	PF00498	0.303
LIG_FHA_2	574	580	PF00498	0.348
LIG_LIR_Apic_2	237	243	PF02991	0.291
LIG_LIR_Apic_2	269	273	PF02991	0.260
LIG_LIR_Gen_1	369	378	PF02991	0.378
LIG_LIR_Nem_3	148	153	PF02991	0.369
LIG_LIR_Nem_3	258	264	PF02991	0.320
LIG_LIR_Nem_3	296	302	PF02991	0.273
LIG_LIR_Nem_3	369	373	PF02991	0.377
LIG_Pex14_1	236	240	PF04695	0.288
LIG_Pex14_2	150	154	PF04695	0.321
LIG_Pex14_2	531	535	PF04695	0.308
LIG_Pex14_2	60	64	PF04695	0.354
LIG_Rb_LxCxE_1	249	269	PF01857	0.302
LIG_Rb_pABgroove_1	296	304	PF01858	0.325
LIG_REV1ctd_RIR_1	147	156	PF16727	0.341
LIG_SH2_CRK	264	268	PF00017	0.324
LIG_SH2_NCK_1	283	287	PF00017	0.260
LIG_SH2_NCK_1	302	306	PF00017	0.260
LIG_SH2_SRC	283	286	PF00017	0.299
LIG_SH2_SRC	302	305	PF00017	0.299
LIG_SH2_STAT5	153	156	PF00017	0.326
LIG_SH2_STAT5	168	171	PF00017	0.334
LIG_SH2_STAT5	264	267	PF00017	0.380
LIG_SH2_STAT5	380	383	PF00017	0.421
LIG_SH2_STAT5	55	58	PF00017	0.360
LIG_SH2_STAT5	86	89	PF00017	0.494
LIG_SH3_3	173	179	PF00018	0.443
LIG_SH3_3	41	47	PF00018	0.456
LIG_SUMO_SIM_anti_2	306	312	PF11976	0.294
LIG_SUMO_SIM_anti_2	442	451	PF11976	0.386
LIG_SUMO_SIM_par_1	442	451	PF11976	0.446
LIG_SUMO_SIM_par_1	570	576	PF11976	0.410
LIG_TRAF2_1	304	307	PF00917	0.182
LIG_UBA3_1	187	192	PF00899	0.259
LIG_UBA3_1	290	297	PF00899	0.234
MOD_CK1_1	128	134	PF00069	0.618
MOD_CK1_1	216	222	PF00069	0.425
MOD_CK1_1	269	275	PF00069	0.260
MOD_CK1_1	480	486	PF00069	0.478
MOD_CK2_1	142	148	PF00069	0.328
MOD_Cter_Amidation	99	102	PF01082	0.511
MOD_DYRK1A_RPxSP_1	78	82	PF00069	0.467
MOD_GlcNHglycan	17	20	PF01048	0.443
MOD_GlcNHglycan	250	253	PF01048	0.261
MOD_GlcNHglycan	28	31	PF01048	0.495
MOD_GlcNHglycan	3	6	PF01048	0.433
MOD_GlcNHglycan	362	365	PF01048	0.260
MOD_GlcNHglycan	376	379	PF01048	0.328
MOD_GlcNHglycan	475	478	PF01048	0.489
MOD_GlcNHglycan	517	520	PF01048	0.427
MOD_GlcNHglycan	587	590	PF01048	0.636
MOD_GSK3_1	101	108	PF00069	0.615
MOD_GSK3_1	120	127	PF00069	0.509
MOD_GSK3_1	193	200	PF00069	0.446
MOD_GSK3_1	22	29	PF00069	0.518
MOD_GSK3_1	269	276	PF00069	0.260
MOD_GSK3_1	309	316	PF00069	0.260
MOD_GSK3_1	323	330	PF00069	0.372
MOD_GSK3_1	348	355	PF00069	0.275
MOD_GSK3_1	374	381	PF00069	0.249
MOD_GSK3_1	441	448	PF00069	0.383
MOD_GSK3_1	469	476	PF00069	0.459
MOD_GSK3_1	538	545	PF00069	0.340
MOD_GSK3_1	80	87	PF00069	0.544
MOD_LATS_1	490	496	PF00433	0.243
MOD_N-GLC_1	25	30	PF02516	0.519
MOD_N-GLC_1	327	332	PF02516	0.182
MOD_N-GLC_1	73	78	PF02516	0.435
MOD_NEK2_1	1	6	PF00069	0.437
MOD_NEK2_1	124	129	PF00069	0.525
MOD_NEK2_1	141	146	PF00069	0.308
MOD_NEK2_1	159	164	PF00069	0.323
MOD_NEK2_1	246	251	PF00069	0.520
MOD_NEK2_1	358	363	PF00069	0.260
MOD_NEK2_1	374	379	PF00069	0.335
MOD_NEK2_1	412	417	PF00069	0.473
MOD_NEK2_1	473	478	PF00069	0.448
MOD_NEK2_1	542	547	PF00069	0.389
MOD_NEK2_1	573	578	PF00069	0.370
MOD_NEK2_1	88	93	PF00069	0.559
MOD_NEK2_2	342	347	PF00069	0.335
MOD_NEK2_2	461	466	PF00069	0.477
MOD_PIKK_1	105	111	PF00454	0.479
MOD_PIKK_1	128	134	PF00454	0.618
MOD_PIKK_1	273	279	PF00454	0.260
MOD_PKA_1	101	107	PF00069	0.757
MOD_PKA_1	125	131	PF00069	0.517
MOD_PKA_1	213	219	PF00069	0.444
MOD_PKA_1	428	434	PF00069	0.463
MOD_PKA_2	101	107	PF00069	0.499
MOD_PKA_2	124	130	PF00069	0.449
MOD_PKA_2	213	219	PF00069	0.444
MOD_PKA_2	312	318	PF00069	0.298
MOD_PKA_2	342	348	PF00069	0.294
MOD_PKA_2	412	418	PF00069	0.473
MOD_PKA_2	428	434	PF00069	0.468
MOD_PKA_2	515	521	PF00069	0.342
MOD_PKB_1	8	16	PF00069	0.421
MOD_Plk_1	327	333	PF00069	0.182
MOD_Plk_1	441	447	PF00069	0.380
MOD_Plk_4	266	272	PF00069	0.260
MOD_Plk_4	441	447	PF00069	0.405
MOD_Plk_4	538	544	PF00069	0.308
MOD_Plk_4	546	552	PF00069	0.252
MOD_ProDKin_1	269	275	PF00069	0.260
MOD_ProDKin_1	323	329	PF00069	0.325
MOD_ProDKin_1	78	84	PF00069	0.518
MOD_SUMO_for_1	116	119	PF00179	0.569
TRG_DiLeu_BaEn_1	442	447	PF01217	0.377
TRG_DiLeu_BaEn_1	568	573	PF01217	0.423
TRG_DiLeu_BaEn_4	306	312	PF01217	0.182
TRG_DiLeu_BaLyEn_6	44	49	PF01217	0.428
TRG_DiLeu_BaLyEn_6	489	494	PF01217	0.446
TRG_ENDOCYTIC_2	264	267	PF00928	0.364
TRG_ENDOCYTIC_2	283	286	PF00928	0.250
TRG_ENDOCYTIC_2	55	58	PF00928	0.360
TRG_ER_diArg_1	34	36	PF00400	0.489
TRG_ER_diArg_1	8	11	PF00400	0.420
TRG_NLS_Bipartite_1	101	118	PF00514	0.584
TRG_NLS_MonoExtC_3	113	119	PF00514	0.496
TRG_NLS_MonoExtC_3	5	10	PF00514	0.412
TRG_NLS_MonoExtN_4	210	216	PF00514	0.448
TRG_NLS_MonoExtN_4	6	11	PF00514	0.404
TRG_Pf-PMV_PEXEL_1	434	438	PF00026	0.303

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDT4	Leptomonas seymouri	73%	100%
A0A1X0NJI1	Trypanosomatidae	54%	100%
A0A3S7X447	Leishmania donovani	81%	100%
A3KP59	Danio rerio	29%	100%
A4I6B6	Leishmania infantum	81%	100%
A5PK74	Bos taurus	28%	89%
A8QFQ3	Brugia malayi	27%	84%
A8XEA2	Caenorhabditis briggsae	23%	77%
B0WMG3	Culex quinquefasciatus	28%	92%
B3MSI4	Drosophila ananassae	29%	71%
B3NU20	Drosophila erecta	29%	91%
B4GUZ2	Drosophila persimilis	28%	87%
B4I100	Drosophila sechellia	30%	91%
B4L6Q5	Drosophila mojavensis	29%	67%
B4NP88	Drosophila willistoni	29%	78%
B4Q068	Drosophila yakuba	29%	87%
B4R4H1	Drosophila simulans	30%	70%
C3XRY1	Branchiostoma floridae	27%	98%
D3ZU57	Rattus norvegicus	29%	100%
E9B1H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	78%	95%
O01658	Caenorhabditis elegans	25%	80%
Q16W06	Aedes aegypti	27%	94%
Q4Q6P0	Leishmania major	79%	100%
Q54K96	Dictyostelium discoideum	31%	100%
Q5ZMM1	Gallus gallus	29%	99%
Q7K4H4	Drosophila melanogaster	28%	91%
Q9H6W3	Homo sapiens	28%	93%
Q9JJF3	Mus musculus	29%	99%
V5AYK7	Trypanosoma cruzi	52%	100%