Cleavage and polyadenylation specificity factor subunit 2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HIZ6_LEIBR

TriTrypDb:

LbrM.31.0320 , LBRM2903_310009900

Length:

522

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HIZ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIZ6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation	8	1
GO:0006378	mRNA polyadenylation	7	1
GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation	9	1
GO:0006397	mRNA processing	7	1
GO:0006399	tRNA metabolic process	7	1
GO:0008033	tRNA processing	8	1
GO:0008380	RNA splicing	7	1
GO:0016071	mRNA metabolic process	6	1
GO:0031123	RNA 3'-end processing	7	1
GO:0031124	mRNA 3'-end processing	8	1
GO:0034470	ncRNA processing	7	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043631	RNA polyadenylation	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0019205	nucleobase-containing compound kinase activity	5	12
GO:0051731	polynucleotide 5'-hydroxyl-kinase activity	5	12
GO:0000166	nucleotide binding	3	2
GO:0005488	binding	1	2
GO:0005524	ATP binding	5	2
GO:0017076	purine nucleotide binding	4	2
GO:0030554	adenyl nucleotide binding	5	2
GO:0032553	ribonucleotide binding	3	2
GO:0032555	purine ribonucleotide binding	4	2
GO:0032559	adenyl ribonucleotide binding	5	2
GO:0035639	purine ribonucleoside triphosphate binding	4	2
GO:0036094	small molecule binding	2	2
GO:0043167	ion binding	2	2
GO:0043168	anion binding	3	2
GO:0097159	organic cyclic compound binding	2	2
GO:0097367	carbohydrate derivative binding	2	2
GO:1901265	nucleoside phosphate binding	3	2
GO:1901363	heterocyclic compound binding	2	2
GO:0046404	polydeoxyribonucleotide 5'-hydroxyl-kinase activity	7	1
GO:0051733	polydeoxyribonucleotide kinase activity	6	1
GO:0051734	polynucleotide kinase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	32	34	PF00675	0.452
CLV_PCSK_KEX2_1	371	373	PF00082	0.313
CLV_PCSK_PC1ET2_1	371	373	PF00082	0.404
CLV_PCSK_SKI1_1	101	105	PF00082	0.413
CLV_PCSK_SKI1_1	126	130	PF00082	0.403
CLV_PCSK_SKI1_1	17	21	PF00082	0.431
CLV_PCSK_SKI1_1	352	356	PF00082	0.504
CLV_PCSK_SKI1_1	400	404	PF00082	0.436
DOC_CKS1_1	163	168	PF01111	0.487
DOC_CKS1_1	380	385	PF01111	0.534
DOC_CYCLIN_yCln2_LP_2	163	169	PF00134	0.487
DOC_MAPK_FxFP_2	168	171	PF00069	0.340
DOC_MAPK_gen_1	230	238	PF00069	0.340
DOC_MAPK_gen_1	475	485	PF00069	0.400
DOC_MAPK_MEF2A_6	120	129	PF00069	0.526
DOC_PP1_RVXF_1	124	130	PF00149	0.427
DOC_PP2B_LxvP_1	80	83	PF13499	0.439
DOC_PP4_FxxP_1	168	171	PF00568	0.340
DOC_PP4_FxxP_1	380	383	PF00568	0.532
DOC_USP7_MATH_1	257	261	PF00917	0.662
DOC_USP7_MATH_1	264	268	PF00917	0.709
DOC_USP7_MATH_1	270	274	PF00917	0.707
DOC_USP7_MATH_1	432	436	PF00917	0.453
DOC_USP7_MATH_1	64	68	PF00917	0.383
DOC_WW_Pin1_4	162	167	PF00397	0.487
DOC_WW_Pin1_4	189	194	PF00397	0.452
DOC_WW_Pin1_4	266	271	PF00397	0.687
DOC_WW_Pin1_4	379	384	PF00397	0.527
DOC_WW_Pin1_4	489	494	PF00397	0.503
LIG_14-3-3_CanoR_1	17	23	PF00244	0.384
LIG_14-3-3_CanoR_1	336	340	PF00244	0.483
LIG_14-3-3_CanoR_1	400	405	PF00244	0.492
LIG_14-3-3_CanoR_1	495	499	PF00244	0.439
LIG_14-3-3_CanoR_1	89	99	PF00244	0.435
LIG_APCC_ABBA_1	10	15	PF00400	0.277
LIG_BRCT_BRCA1_1	466	470	PF00533	0.336
LIG_Clathr_ClatBox_1	353	357	PF01394	0.286
LIG_Clathr_ClatBox_1	391	395	PF01394	0.473
LIG_EH1_1	211	219	PF00400	0.452
LIG_FHA_1	190	196	PF00498	0.427
LIG_FHA_1	280	286	PF00498	0.529
LIG_FHA_1	356	362	PF00498	0.493
LIG_FHA_1	382	388	PF00498	0.453
LIG_FHA_1	495	501	PF00498	0.454
LIG_FHA_1	50	56	PF00498	0.355
LIG_FHA_1	75	81	PF00498	0.405
LIG_FHA_2	285	291	PF00498	0.430
LIG_FHA_2	43	49	PF00498	0.401
LIG_FHA_2	436	442	PF00498	0.516
LIG_FHA_2	451	457	PF00498	0.338
LIG_FHA_2	514	520	PF00498	0.280
LIG_LIR_Apic_2	165	171	PF02991	0.487
LIG_LIR_Apic_2	334	340	PF02991	0.564
LIG_LIR_Gen_1	435	444	PF02991	0.532
LIG_LIR_Gen_1	467	477	PF02991	0.306
LIG_LIR_Nem_3	239	243	PF02991	0.369
LIG_LIR_Nem_3	435	439	PF02991	0.522
LIG_LIR_Nem_3	467	473	PF02991	0.320
LIG_NRBOX	510	516	PF00104	0.484
LIG_Pex14_1	144	148	PF04695	0.452
LIG_Pex14_1	375	379	PF04695	0.387
LIG_SH2_CRK	154	158	PF00017	0.340
LIG_SH2_PTP2	196	199	PF00017	0.224
LIG_SH2_STAP1	91	95	PF00017	0.487
LIG_SH2_STAT5	141	144	PF00017	0.332
LIG_SH2_STAT5	196	199	PF00017	0.411
LIG_SH2_STAT5	378	381	PF00017	0.399
LIG_SH2_STAT5	8	11	PF00017	0.437
LIG_SH3_2	383	388	PF14604	0.559
LIG_SH3_3	201	207	PF00018	0.478
LIG_SH3_3	239	245	PF00018	0.494
LIG_SH3_3	380	386	PF00018	0.460
LIG_SH3_3	53	59	PF00018	0.355
LIG_SH3_3	6	12	PF00018	0.509
LIG_SH3_3	78	84	PF00018	0.371
LIG_SUMO_SIM_anti_2	494	501	PF11976	0.427
LIG_SUMO_SIM_anti_2	77	82	PF11976	0.452
LIG_SUMO_SIM_par_1	155	160	PF11976	0.340
LIG_SUMO_SIM_par_1	390	395	PF11976	0.477
LIG_SUMO_SIM_par_1	446	453	PF11976	0.422
LIG_SUMO_SIM_par_1	496	501	PF11976	0.525
LIG_TRAF2_1	516	519	PF00917	0.413
LIG_TRFH_1	8	12	PF08558	0.407
LIG_TYR_ITIM	152	157	PF00017	0.340
LIG_ULM_U2AF65_1	371	376	PF00076	0.275
MOD_CDC14_SPxK_1	385	388	PF00782	0.556
MOD_CDK_SPxK_1	382	388	PF00069	0.551
MOD_CDK_SPxK_1	489	495	PF00069	0.504
MOD_CK1_1	21	27	PF00069	0.452
MOD_CK1_1	277	283	PF00069	0.639
MOD_CK1_1	28	34	PF00069	0.452
MOD_CK1_1	42	48	PF00069	0.253
MOD_CK1_1	435	441	PF00069	0.498
MOD_CK1_1	452	458	PF00069	0.290
MOD_CK1_1	69	75	PF00069	0.435
MOD_CK2_1	105	111	PF00069	0.327
MOD_CK2_1	42	48	PF00069	0.414
MOD_CK2_1	450	456	PF00069	0.404
MOD_CK2_1	468	474	PF00069	0.537
MOD_CK2_1	513	519	PF00069	0.399
MOD_CMANNOS	307	310	PF00535	0.496
MOD_GlcNHglycan	266	269	PF01048	0.733
MOD_GlcNHglycan	272	275	PF01048	0.772
MOD_GlcNHglycan	500	503	PF01048	0.579
MOD_GSK3_1	17	24	PF00069	0.441
MOD_GSK3_1	258	265	PF00069	0.650
MOD_GSK3_1	266	273	PF00069	0.731
MOD_GSK3_1	284	291	PF00069	0.546
MOD_GSK3_1	361	368	PF00069	0.519
MOD_GSK3_1	390	397	PF00069	0.434
MOD_GSK3_1	464	471	PF00069	0.473
MOD_GSK3_1	494	501	PF00069	0.445
MOD_GSK3_1	66	73	PF00069	0.402
MOD_GSK3_1	91	98	PF00069	0.389
MOD_N-GLC_1	189	194	PF02516	0.452
MOD_NEK2_1	22	27	PF00069	0.359
MOD_NEK2_1	288	293	PF00069	0.434
MOD_NEK2_1	355	360	PF00069	0.522
MOD_NEK2_1	361	366	PF00069	0.586
MOD_NEK2_1	394	399	PF00069	0.485
MOD_NEK2_1	468	473	PF00069	0.508
MOD_NEK2_1	487	492	PF00069	0.398
MOD_NEK2_1	498	503	PF00069	0.375
MOD_NEK2_1	71	76	PF00069	0.370
MOD_NEK2_1	95	100	PF00069	0.437
MOD_PIKK_1	22	28	PF00454	0.452
MOD_PIKK_1	277	283	PF00454	0.538
MOD_PIKK_1	394	400	PF00454	0.524
MOD_PK_1	66	72	PF00069	0.452
MOD_PKA_2	335	341	PF00069	0.494
MOD_PKA_2	494	500	PF00069	0.446
MOD_Plk_1	322	328	PF00069	0.401
MOD_Plk_1	394	400	PF00069	0.432
MOD_Plk_1	473	479	PF00069	0.439
MOD_Plk_4	137	143	PF00069	0.338
MOD_Plk_4	284	290	PF00069	0.464
MOD_Plk_4	303	309	PF00069	0.317
MOD_Plk_4	365	371	PF00069	0.316
MOD_Plk_4	387	393	PF00069	0.442
MOD_Plk_4	411	417	PF00069	0.603
MOD_Plk_4	432	438	PF00069	0.398
MOD_Plk_4	478	484	PF00069	0.510
MOD_Plk_4	494	500	PF00069	0.422
MOD_Plk_4	66	72	PF00069	0.379
MOD_Plk_4	74	80	PF00069	0.308
MOD_Plk_4	95	101	PF00069	0.416
MOD_ProDKin_1	162	168	PF00069	0.487
MOD_ProDKin_1	189	195	PF00069	0.452
MOD_ProDKin_1	266	272	PF00069	0.691
MOD_ProDKin_1	379	385	PF00069	0.527
MOD_ProDKin_1	489	495	PF00069	0.504
MOD_SUMO_rev_2	517	522	PF00179	0.326
TRG_DiLeu_BaEn_1	494	499	PF01217	0.552
TRG_DiLeu_BaEn_4	186	192	PF01217	0.452
TRG_DiLeu_BaLyEn_6	153	158	PF01217	0.340
TRG_ENDOCYTIC_2	154	157	PF00928	0.352
TRG_ENDOCYTIC_2	196	199	PF00928	0.487
TRG_ENDOCYTIC_2	240	243	PF00928	0.252
TRG_ER_diArg_1	112	115	PF00400	0.415
TRG_ER_diArg_1	230	233	PF00400	0.324
TRG_ER_diArg_1	372	375	PF00400	0.431
TRG_Pf-PMV_PEXEL_1	101	105	PF00026	0.383
TRG_Pf-PMV_PEXEL_1	475	479	PF00026	0.495

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA46	Leptomonas seymouri	76%	100%
A0A1X0NKV9	Trypanosomatidae	47%	100%
A0A3S7X444	Leishmania donovani	91%	100%
A0A422MZS5	Trypanosoma rangeli	47%	100%
A4I6B3	Leishmania infantum	91%	100%
C9ZN71	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B1G9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9B1H1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4Q6P3	Leishmania major	90%	100%
V5BBI5	Trypanosoma cruzi	47%	100%