Ubiquitin carboxyl-terminal hydrolase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin carboxyl-terminal hydrolase, putative

Species:

Leishmania braziliensis

UniProt:

A4HIX8_LEIBR

TriTrypDb:

LbrM.31.0140 , LBRM2903_310009100

Length:

499

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HIX8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIX8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006511	ubiquitin-dependent protein catabolic process	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009056	catabolic process	2	11
GO:0009057	macromolecule catabolic process	4	11
GO:0009987	cellular process	1	11
GO:0010498	proteasomal protein catabolic process	5	11
GO:0016579	protein deubiquitination	6	11
GO:0019538	protein metabolic process	3	11
GO:0019941	modification-dependent protein catabolic process	6	11
GO:0030163	protein catabolic process	4	11
GO:0036211	protein modification process	4	11
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0043632	modification-dependent macromolecule catabolic process	5	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044248	cellular catabolic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044265	obsolete cellular macromolecule catabolic process	4	11
GO:0051603	proteolysis involved in protein catabolic process	5	11
GO:0070646	protein modification by small protein removal	5	11
GO:0070647	protein modification by small protein conjugation or removal	5	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901565	organonitrogen compound catabolic process	4	11
GO:1901575	organic substance catabolic process	3	11
GO:0009894	regulation of catabolic process	4	1
GO:0019222	regulation of metabolic process	3	1
GO:0030162	regulation of proteolysis	6	1
GO:0042176	regulation of protein catabolic process	5	1
GO:0050789	regulation of biological process	2	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061136	regulation of proteasomal protein catabolic process	6	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1903050	regulation of proteolysis involved in protein catabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004843	cysteine-type deubiquitinase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0019783	ubiquitin-like protein peptidase activity	4	11
GO:0101005	deubiquitinase activity	5	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0005488	binding	1	1
GO:0044877	protein-containing complex binding	2	1
GO:0070628	proteasome binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	3	5	PF00675	0.510
CLV_NRD_NRD_1	385	387	PF00675	0.326
CLV_NRD_NRD_1	394	396	PF00675	0.339
CLV_NRD_NRD_1	446	448	PF00675	0.205
CLV_NRD_NRD_1	469	471	PF00675	0.205
CLV_PCSK_FUR_1	392	396	PF00082	0.379
CLV_PCSK_KEX2_1	3	5	PF00082	0.510
CLV_PCSK_KEX2_1	385	387	PF00082	0.325
CLV_PCSK_KEX2_1	392	394	PF00082	0.333
CLV_PCSK_KEX2_1	469	471	PF00082	0.313
CLV_PCSK_SKI1_1	171	175	PF00082	0.386
CLV_PCSK_SKI1_1	347	351	PF00082	0.309
CLV_PCSK_SKI1_1	35	39	PF00082	0.631
CLV_PCSK_SKI1_1	353	357	PF00082	0.297
CLV_PCSK_SKI1_1	379	383	PF00082	0.274
CLV_PCSK_SKI1_1	4	8	PF00082	0.509
CLV_PCSK_SKI1_1	69	73	PF00082	0.331
CLV_PCSK_SKI1_1	89	93	PF00082	0.128
DEG_COP1_1	115	125	PF00400	0.647
DEG_Nend_UBRbox_3	1	3	PF02207	0.510
DEG_SCF_FBW7_2	404	411	PF00400	0.205
DOC_CYCLIN_RxL_1	32	40	PF00134	0.450
DOC_MAPK_DCC_7	198	206	PF00069	0.356
DOC_MAPK_DCC_7	347	357	PF00069	0.315
DOC_MAPK_gen_1	271	279	PF00069	0.425
DOC_MAPK_gen_1	347	357	PF00069	0.315
DOC_MAPK_gen_1	49	59	PF00069	0.460
DOC_MAPK_gen_1	73	80	PF00069	0.496
DOC_MAPK_MEF2A_6	198	206	PF00069	0.386
DOC_MAPK_MEF2A_6	35	43	PF00069	0.606
DOC_MAPK_MEF2A_6	52	61	PF00069	0.450
DOC_PP1_RVXF_1	351	358	PF00149	0.298
DOC_PP1_RVXF_1	45	51	PF00149	0.313
DOC_PP4_FxxP_1	305	308	PF00568	0.425
DOC_USP7_MATH_1	20	24	PF00917	0.508
DOC_USP7_MATH_1	241	245	PF00917	0.361
DOC_USP7_MATH_1	364	368	PF00917	0.205
DOC_USP7_MATH_1	461	465	PF00917	0.425
DOC_USP7_MATH_1	90	94	PF00917	0.427
DOC_USP7_UBL2_3	372	376	PF12436	0.375
DOC_USP7_UBL2_3	69	73	PF12436	0.540
DOC_WW_Pin1_4	10	15	PF00397	0.503
DOC_WW_Pin1_4	400	405	PF00397	0.449
LIG_14-3-3_CanoR_1	185	194	PF00244	0.205
LIG_14-3-3_CanoR_1	304	308	PF00244	0.453
LIG_14-3-3_CanoR_1	338	342	PF00244	0.368
LIG_14-3-3_CanoR_1	35	40	PF00244	0.590
LIG_14-3-3_CanoR_1	9	14	PF00244	0.516
LIG_Actin_WH2_2	358	374	PF00022	0.389
LIG_Actin_WH2_2	456	474	PF00022	0.425
LIG_Actin_WH2_2	54	71	PF00022	0.470
LIG_BRCT_BRCA1_1	28	32	PF00533	0.468
LIG_eIF4E_1	331	337	PF01652	0.235
LIG_FHA_1	107	113	PF00498	0.474
LIG_FHA_1	152	158	PF00498	0.425
LIG_FHA_1	206	212	PF00498	0.373
LIG_FHA_1	285	291	PF00498	0.395
LIG_FHA_1	36	42	PF00498	0.540
LIG_FHA_1	361	367	PF00498	0.315
LIG_FHA_1	54	60	PF00498	0.487
LIG_FHA_2	164	170	PF00498	0.205
LIG_FHA_2	214	220	PF00498	0.356
LIG_FHA_2	261	267	PF00498	0.376
LIG_FHA_2	90	96	PF00498	0.449
LIG_Integrin_RGD_1	178	180	PF01839	0.425
LIG_LIR_Apic_2	302	308	PF02991	0.425
LIG_LIR_Gen_1	248	258	PF02991	0.419
LIG_LIR_Gen_1	276	286	PF02991	0.333
LIG_LIR_Gen_1	330	339	PF02991	0.347
LIG_LIR_Nem_3	248	253	PF02991	0.419
LIG_LIR_Nem_3	276	282	PF02991	0.313
LIG_LIR_Nem_3	330	336	PF02991	0.328
LIG_LIR_Nem_3	356	360	PF02991	0.303
LIG_LIR_Nem_3	420	426	PF02991	0.317
LIG_LIR_Nem_3	450	455	PF02991	0.329
LIG_MAD2	350	358	PF02301	0.313
LIG_NRBOX	234	240	PF00104	0.386
LIG_PDZ_Class_2	494	499	PF00595	0.433
LIG_Pex14_2	301	305	PF04695	0.425
LIG_PTB_Apo_2	150	157	PF02174	0.313
LIG_PTB_Apo_2	211	218	PF02174	0.356
LIG_PTB_Phospho_1	150	156	PF10480	0.313
LIG_SH2_GRB2like	156	159	PF00017	0.313
LIG_SH2_PTP2	331	334	PF00017	0.458
LIG_SH2_PTP2	481	484	PF00017	0.425
LIG_SH2_SRC	156	159	PF00017	0.425
LIG_SH2_STAP1	250	254	PF00017	0.425
LIG_SH2_STAT5	156	159	PF00017	0.351
LIG_SH2_STAT5	331	334	PF00017	0.326
LIG_SH2_STAT5	442	445	PF00017	0.313
LIG_SH2_STAT5	481	484	PF00017	0.313
LIG_SH3_3	117	123	PF00018	0.487
LIG_SH3_3	16	22	PF00018	0.515
LIG_SH3_3	262	268	PF00018	0.458
LIG_SH3_3	492	498	PF00018	0.490
LIG_SUMO_SIM_anti_2	109	115	PF11976	0.474
LIG_SUMO_SIM_anti_2	38	43	PF11976	0.406
LIG_SUMO_SIM_par_1	35	40	PF11976	0.675
LIG_TRAF2_1	408	411	PF00917	0.414
LIG_TYR_ITIM	479	484	PF00017	0.425
LIG_UBA3_1	42	49	PF00899	0.333
LIG_UBA3_1	85	89	PF00899	0.474
MOD_CDK_SPxxK_3	10	17	PF00069	0.510
MOD_CK1_1	10	16	PF00069	0.506
MOD_CK1_1	115	121	PF00069	0.657
MOD_CK1_1	193	199	PF00069	0.298
MOD_CK1_1	323	329	PF00069	0.351
MOD_CK2_1	163	169	PF00069	0.397
MOD_CK2_1	193	199	PF00069	0.301
MOD_CK2_1	213	219	PF00069	0.290
MOD_Cter_Amidation	445	448	PF01082	0.425
MOD_GlcNHglycan	114	117	PF01048	0.398
MOD_GlcNHglycan	158	161	PF01048	0.386
MOD_GlcNHglycan	187	190	PF01048	0.205
MOD_GlcNHglycan	420	423	PF01048	0.320
MOD_GlcNHglycan	435	438	PF01048	0.313
MOD_GlcNHglycan	99	102	PF01048	0.348
MOD_GSK3_1	132	139	PF00069	0.567
MOD_GSK3_1	190	197	PF00069	0.315
MOD_GSK3_1	206	213	PF00069	0.463
MOD_GSK3_1	280	287	PF00069	0.364
MOD_GSK3_1	360	367	PF00069	0.396
MOD_GSK3_1	4	11	PF00069	0.513
MOD_GSK3_1	400	407	PF00069	0.367
MOD_LATS_1	33	39	PF00433	0.452
MOD_N-GLC_1	132	137	PF02516	0.602
MOD_N-GLC_1	284	289	PF02516	0.398
MOD_N-GLC_1	418	423	PF02516	0.301
MOD_N-GLC_2	153	155	PF02516	0.313
MOD_NEK2_1	138	143	PF00069	0.543
MOD_NEK2_1	163	168	PF00069	0.404
MOD_NEK2_1	206	211	PF00069	0.301
MOD_NEK2_1	258	263	PF00069	0.337
MOD_NEK2_1	28	33	PF00069	0.468
MOD_NEK2_1	37	42	PF00069	0.619
MOD_NEK2_1	484	489	PF00069	0.373
MOD_NEK2_1	61	66	PF00069	0.468
MOD_PIKK_1	228	234	PF00454	0.356
MOD_PIKK_1	26	32	PF00454	0.474
MOD_PKA_2	2	8	PF00069	0.508
MOD_PKA_2	303	309	PF00069	0.456
MOD_PKA_2	320	326	PF00069	0.228
MOD_PKA_2	337	343	PF00069	0.325
MOD_PKA_2	399	405	PF00069	0.234
MOD_Plk_1	53	59	PF00069	0.485
MOD_Plk_1	95	101	PF00069	0.475
MOD_Plk_2-3	289	295	PF00069	0.355
MOD_Plk_2-3	53	59	PF00069	0.564
MOD_Plk_4	106	112	PF00069	0.474
MOD_Plk_4	206	212	PF00069	0.362
MOD_Plk_4	241	247	PF00069	0.349
MOD_Plk_4	37	43	PF00069	0.411
MOD_ProDKin_1	10	16	PF00069	0.506
MOD_ProDKin_1	400	406	PF00069	0.449
MOD_SUMO_for_1	417	420	PF00179	0.446
MOD_SUMO_rev_2	40	48	PF00179	0.333
TRG_DiLeu_BaEn_1	81	86	PF01217	0.540
TRG_DiLeu_BaEn_2	221	227	PF01217	0.425
TRG_DiLeu_BaLyEn_6	32	37	PF01217	0.459
TRG_DiLeu_LyEn_5	411	416	PF01217	0.341
TRG_ENDOCYTIC_2	250	253	PF00928	0.425
TRG_ENDOCYTIC_2	331	334	PF00928	0.326
TRG_ENDOCYTIC_2	423	426	PF00928	0.313
TRG_ENDOCYTIC_2	481	484	PF00928	0.313
TRG_ER_diArg_1	392	395	PF00400	0.397
TRG_ER_diArg_1	468	470	PF00400	0.396
TRG_ER_diArg_1	485	488	PF00400	0.354
TRG_Pf-PMV_PEXEL_1	165	169	PF00026	0.425
TRG_Pf-PMV_PEXEL_1	379	383	PF00026	0.397

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMH5	Leptomonas seymouri	77%	100%
A0A1X0NJ77	Trypanosomatidae	51%	100%
A0A3R7RDZ6	Trypanosoma rangeli	51%	100%
A0A3S7X418	Leishmania donovani	87%	100%
A5D9H7	Bos taurus	22%	100%
C9ZN32	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
E9AHK7	Leishmania infantum	87%	100%
E9B1G2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
F1M625	Rattus norvegicus	22%	100%
O75317	Homo sapiens	22%	100%
P40826	Oryctolagus cuniculus	32%	100%
P43593	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
P54578	Homo sapiens	31%	100%
P60051	Pan troglodytes	31%	100%
Q0IIF7	Bos taurus	32%	100%
Q17361	Caenorhabditis elegans	30%	100%
Q3LFD5	Homo sapiens	26%	100%
Q4Q6Q0	Leishmania major	85%	100%
Q52KZ6	Xenopus laevis	23%	100%
Q84WC6	Arabidopsis thaliana	29%	100%
Q92353	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
Q949Y0	Arabidopsis thaliana	29%	100%
Q9JMA1	Mus musculus	32%	100%
Q9UMW8	Homo sapiens	26%	100%
Q9WTV6	Mus musculus	25%	100%
V5AT19	Trypanosoma cruzi	24%	93%
V5BIL0	Trypanosoma cruzi	51%	100%