tRNA-intron lyase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

tRNA-intron lyase

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4HIT3_LEIBR

TriTrypDb:

LbrM.30.3490 , LBRM2903_300042000

Length:

143

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	3
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

A4HIT3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIT3

Function

Biological processes
Term	Name	Level	Count
GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation	8	3
GO:0006139	nucleobase-containing compound metabolic process	3	3
GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation	9	3
GO:0006396	RNA processing	6	3
GO:0006399	tRNA metabolic process	7	3
GO:0006725	cellular aromatic compound metabolic process	3	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008033	tRNA processing	8	3
GO:0008152	metabolic process	1	3
GO:0008380	RNA splicing	7	3
GO:0009987	cellular process	1	3
GO:0016070	RNA metabolic process	5	3
GO:0034470	ncRNA processing	7	3
GO:0034641	cellular nitrogen compound metabolic process	3	3
GO:0034660	ncRNA metabolic process	6	3
GO:0043170	macromolecule metabolic process	3	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0046483	heterocycle metabolic process	3	3
GO:0071704	organic substance metabolic process	2	3
GO:0090304	nucleic acid metabolic process	4	3
GO:1901360	organic cyclic compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	3
GO:0005488	binding	1	3
GO:0097159	organic cyclic compound binding	2	3
GO:1901363	heterocyclic compound binding	2	3
GO:0000213	tRNA-intron endonuclease activity	6	1
GO:0003824	catalytic activity	1	1
GO:0004518	nuclease activity	4	1
GO:0004519	endonuclease activity	5	1
GO:0004521	RNA endonuclease activity	5	1
GO:0004540	RNA nuclease activity	4	1
GO:0004549	tRNA-specific ribonuclease activity	5	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0016892	RNA endonuclease activity, producing 3'-phosphomonoesters	6	1
GO:0016894	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters	6	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	28	32	PF00656	0.487
CLV_NRD_NRD_1	121	123	PF00675	0.399
CLV_NRD_NRD_1	72	74	PF00675	0.455
CLV_PCSK_KEX2_1	11	13	PF00082	0.516
CLV_PCSK_KEX2_1	72	74	PF00082	0.455
CLV_PCSK_PC1ET2_1	11	13	PF00082	0.527
DEG_SCF_FBW7_2	126	133	PF00400	0.385
DOC_CKS1_1	127	132	PF01111	0.385
DOC_PP4_FxxP_1	102	105	PF00568	0.623
DOC_USP7_MATH_1	87	91	PF00917	0.448
DOC_USP7_MATH_2	13	19	PF00917	0.510
DOC_WW_Pin1_4	126	131	PF00397	0.382
DOC_WW_Pin1_4	33	38	PF00397	0.438
DOC_WW_Pin1_4	50	55	PF00397	0.553
LIG_14-3-3_CanoR_1	116	126	PF00244	0.381
LIG_14-3-3_CanoR_1	72	76	PF00244	0.422
LIG_BIR_III_2	31	35	PF00653	0.414
LIG_FHA_2	84	90	PF00498	0.332
LIG_LIR_Apic_2	100	105	PF02991	0.619
LIG_LIR_Gen_1	3	9	PF02991	0.570
LIG_LIR_Nem_3	3	7	PF02991	0.590
LIG_NRBOX	39	45	PF00104	0.333
LIG_SH2_CRK	139	143	PF00017	0.345
LIG_SH2_STAP1	139	143	PF00017	0.459
LIG_SH2_STAT5	139	142	PF00017	0.411
LIG_SH2_STAT5	4	7	PF00017	0.616
LIG_SH2_STAT5	64	67	PF00017	0.405
LIG_SH3_3	124	130	PF00018	0.373
LIG_SH3_3	31	37	PF00018	0.395
LIG_SUMO_SIM_anti_2	107	113	PF11976	0.468
LIG_SUMO_SIM_anti_2	3	9	PF11976	0.488
LIG_TYR_ITIM	137	142	PF00017	0.316
MOD_CDC14_SPxK_1	53	56	PF00782	0.442
MOD_CDK_SPxK_1	50	56	PF00069	0.436
MOD_CK1_1	107	113	PF00069	0.400
MOD_CK1_1	18	24	PF00069	0.609
MOD_CK1_1	36	42	PF00069	0.414
MOD_CK2_1	83	89	PF00069	0.384
MOD_GlcNHglycan	106	109	PF01048	0.474
MOD_GlcNHglycan	25	30	PF01048	0.518
MOD_GSK3_1	122	129	PF00069	0.419
MOD_GSK3_1	15	22	PF00069	0.661
MOD_GSK3_1	83	90	PF00069	0.468
MOD_N-GLC_2	119	121	PF02516	0.362
MOD_NEK2_1	137	142	PF00069	0.379
MOD_NEK2_1	43	48	PF00069	0.463
MOD_NEK2_1	71	76	PF00069	0.332
MOD_NMyristoyl	1	7	PF02799	0.503
MOD_OFUCOSY	115	121	PF10250	0.377
MOD_PKA_1	122	128	PF00069	0.437
MOD_PKA_2	71	77	PF00069	0.478
MOD_Plk_1	19	25	PF00069	0.431
MOD_Plk_4	107	113	PF00069	0.405
MOD_Plk_4	122	128	PF00069	0.377
MOD_Plk_4	3	9	PF00069	0.583
MOD_ProDKin_1	126	132	PF00069	0.380
MOD_ProDKin_1	33	39	PF00069	0.429
MOD_ProDKin_1	50	56	PF00069	0.552
TRG_ENDOCYTIC_2	139	142	PF00928	0.330
TRG_ENDOCYTIC_2	4	7	PF00928	0.528
TRG_ER_diArg_1	71	73	PF00400	0.305
TRG_Pf-PMV_PEXEL_1	63	67	PF00026	0.279

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4G2	Leptomonas seymouri	57%	91%
A0A1X0P2F0	Trypanosomatidae	39%	97%
A0A3S7X3W7	Leishmania donovani	81%	100%
A4I635	Leishmania infantum	81%	100%
C9ZRF4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	89%
E9B1C0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
Q4Q6U4	Leishmania major	79%	100%