SAM-dependent MTase TRM10-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA (Guanine-1)-methyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4HIP1_LEIBR

TriTrypDb:

LbrM.30.3060 , LBRM2903_300037300

Length:

382

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HIP1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIP1

Function

Biological processes
Term	Name	Level	Count
GO:0008152	metabolic process	1	8
GO:0032259	methylation	2	8
GO:0001510	RNA methylation	4	1
GO:0002939	tRNA N1-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0008168	methyltransferase activity	4	10
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	10
GO:0000049	tRNA binding	5	1
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0005488	binding	1	1
GO:0008173	RNA methyltransferase activity	4	3
GO:0008175	tRNA methyltransferase activity	5	3
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	3
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	3
GO:0016423	tRNA (guanine) methyltransferase activity	6	3
GO:0097159	organic cyclic compound binding	2	1
GO:0140098	catalytic activity, acting on RNA	3	3
GO:0140101	catalytic activity, acting on a tRNA	4	3
GO:0140640	catalytic activity, acting on a nucleic acid	2	3
GO:1901363	heterocyclic compound binding	2	1
GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity	8	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	237	241	PF00656	0.442
CLV_C14_Caspase3-7	24	28	PF00656	0.761
CLV_C14_Caspase3-7	35	39	PF00656	0.678
CLV_C14_Caspase3-7	358	362	PF00656	0.540
CLV_NRD_NRD_1	104	106	PF00675	0.498
CLV_NRD_NRD_1	115	117	PF00675	0.366
CLV_NRD_NRD_1	210	212	PF00675	0.225
CLV_NRD_NRD_1	329	331	PF00675	0.500
CLV_NRD_NRD_1	338	340	PF00675	0.522
CLV_NRD_NRD_1	342	344	PF00675	0.528
CLV_NRD_NRD_1	52	54	PF00675	0.596
CLV_NRD_NRD_1	64	66	PF00675	0.384
CLV_NRD_NRD_1	77	79	PF00675	0.422
CLV_PCSK_FUR_1	338	342	PF00082	0.567
CLV_PCSK_FUR_1	75	79	PF00082	0.441
CLV_PCSK_KEX2_1	104	106	PF00082	0.496
CLV_PCSK_KEX2_1	114	116	PF00082	0.376
CLV_PCSK_KEX2_1	210	212	PF00082	0.225
CLV_PCSK_KEX2_1	329	331	PF00082	0.500
CLV_PCSK_KEX2_1	338	340	PF00082	0.522
CLV_PCSK_KEX2_1	341	343	PF00082	0.529
CLV_PCSK_KEX2_1	52	54	PF00082	0.566
CLV_PCSK_KEX2_1	63	65	PF00082	0.390
CLV_PCSK_KEX2_1	74	76	PF00082	0.351
CLV_PCSK_KEX2_1	77	79	PF00082	0.358
CLV_PCSK_PC1ET2_1	340	342	PF00082	0.530
CLV_PCSK_PC1ET2_1	63	65	PF00082	0.474
CLV_PCSK_PC1ET2_1	74	76	PF00082	0.421
CLV_PCSK_PC7_1	338	344	PF00082	0.450
CLV_PCSK_SKI1_1	293	297	PF00082	0.211
DEG_SPOP_SBC_1	17	21	PF00917	0.472
DOC_USP7_MATH_1	121	125	PF00917	0.379
DOC_USP7_MATH_1	15	19	PF00917	0.615
DOC_USP7_MATH_1	241	245	PF00917	0.356
DOC_USP7_MATH_1	43	47	PF00917	0.567
DOC_USP7_UBL2_3	336	340	PF12436	0.696
DOC_USP7_UBL2_3	70	74	PF12436	0.443
DOC_WW_Pin1_4	312	317	PF00397	0.518
DOC_WW_Pin1_4	365	370	PF00397	0.491
DOC_WW_Pin1_4	372	377	PF00397	0.463
DOC_WW_Pin1_4	8	13	PF00397	0.693
LIG_14-3-3_CanoR_1	104	111	PF00244	0.417
LIG_Actin_WH2_2	153	170	PF00022	0.506
LIG_Actin_WH2_2	296	313	PF00022	0.506
LIG_BIR_II_1	1	5	PF00653	0.477
LIG_BRCT_BRCA1_1	222	226	PF00533	0.506
LIG_EH1_1	298	306	PF00400	0.506
LIG_FHA_1	21	27	PF00498	0.495
LIG_FHA_1	247	253	PF00498	0.446
LIG_FHA_1	275	281	PF00498	0.467
LIG_FHA_1	329	335	PF00498	0.508
LIG_FHA_2	187	193	PF00498	0.452
LIG_FHA_2	241	247	PF00498	0.472
LIG_FHA_2	45	51	PF00498	0.582
LIG_FHA_2	57	63	PF00498	0.451
LIG_LIR_Gen_1	163	172	PF02991	0.425
LIG_LIR_Gen_1	91	100	PF02991	0.340
LIG_LIR_Nem_3	163	167	PF02991	0.449
LIG_LIR_Nem_3	204	209	PF02991	0.414
LIG_LIR_Nem_3	91	96	PF02991	0.340
LIG_PCNA_PIPBox_1	89	98	PF02747	0.327
LIG_Pex14_1	222	226	PF04695	0.425
LIG_Pex14_2	140	144	PF04695	0.264
LIG_RPA_C_Fungi	110	122	PF08784	0.441
LIG_SH2_CRK	164	168	PF00017	0.246
LIG_SH2_NCK_1	267	271	PF00017	0.379
LIG_SH2_STAT5	251	254	PF00017	0.264
LIG_SH3_3	130	136	PF00018	0.453
LIG_SH3_3	170	176	PF00018	0.264
LIG_SH3_3	9	15	PF00018	0.605
LIG_TRAF2_1	349	352	PF00917	0.619
LIG_TRAF2_1	88	91	PF00917	0.479
LIG_TRAF2_1	97	100	PF00917	0.455
LIG_TYR_ITIM	305	310	PF00017	0.379
LIG_UBA3_1	177	184	PF00899	0.179
MOD_CK1_1	155	161	PF00069	0.273
MOD_CK1_1	18	24	PF00069	0.570
MOD_CK1_1	266	272	PF00069	0.417
MOD_CK1_1	365	371	PF00069	0.492
MOD_CK1_1	44	50	PF00069	0.612
MOD_CK2_1	121	127	PF00069	0.532
MOD_CK2_1	184	190	PF00069	0.379
MOD_CK2_1	346	352	PF00069	0.578
MOD_CK2_1	44	50	PF00069	0.568
MOD_CK2_1	56	62	PF00069	0.463
MOD_CK2_1	94	100	PF00069	0.497
MOD_Cter_Amidation	336	339	PF01082	0.557
MOD_GlcNHglycan	164	167	PF01048	0.324
MOD_GlcNHglycan	268	271	PF01048	0.429
MOD_GSK3_1	146	153	PF00069	0.500
MOD_GSK3_1	16	23	PF00069	0.583
MOD_GSK3_1	162	169	PF00069	0.298
MOD_N-GLC_1	365	370	PF02516	0.674
MOD_N-GLC_2	290	292	PF02516	0.246
MOD_NEK2_1	140	145	PF00069	0.292
MOD_NEK2_1	162	167	PF00069	0.248
MOD_PIKK_1	155	161	PF00454	0.246
MOD_PIKK_1	214	220	PF00454	0.311
MOD_PKA_2	103	109	PF00069	0.529
MOD_PKA_2	282	288	PF00069	0.169
MOD_PKA_2	328	334	PF00069	0.465
MOD_Plk_1	106	112	PF00069	0.322
MOD_Plk_1	140	146	PF00069	0.298
MOD_Plk_2-3	346	352	PF00069	0.698
MOD_Plk_2-3	357	363	PF00069	0.764
MOD_Plk_2-3	94	100	PF00069	0.418
MOD_Plk_4	106	112	PF00069	0.322
MOD_Plk_4	177	183	PF00069	0.366
MOD_Plk_4	282	288	PF00069	0.325
MOD_ProDKin_1	312	318	PF00069	0.395
MOD_ProDKin_1	365	371	PF00069	0.492
MOD_ProDKin_1	372	378	PF00069	0.461
MOD_ProDKin_1	8	14	PF00069	0.692
MOD_SUMO_rev_2	94	103	PF00179	0.445
TRG_ENDOCYTIC_2	164	167	PF00928	0.249
TRG_ENDOCYTIC_2	307	310	PF00928	0.379
TRG_ER_diArg_1	114	116	PF00400	0.409
TRG_ER_diArg_1	209	211	PF00400	0.264
TRG_ER_diArg_1	329	332	PF00400	0.468
TRG_ER_diArg_1	338	341	PF00400	0.669
TRG_ER_diArg_1	342	344	PF00400	0.737
TRG_ER_diArg_1	51	53	PF00400	0.656
TRG_ER_diArg_1	64	66	PF00400	0.379
TRG_NLS_Bipartite_1	329	344	PF00514	0.648
TRG_NLS_MonoCore_2	338	343	PF00514	0.490
TRG_NLS_MonoExtC_3	338	343	PF00514	0.564
TRG_NLS_MonoExtN_4	336	343	PF00514	0.550
TRG_Pf-PMV_PEXEL_1	55	60	PF00026	0.364

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I770	Leptomonas seymouri	70%	100%
A0A0S4JPX3	Bodo saltans	39%	100%
A0A1X0P1F8	Trypanosomatidae	48%	100%
A0A3S5ISH7	Trypanosoma rangeli	52%	100%
A0A3S7X3S5	Leishmania donovani	74%	99%
A4I5Y8	Leishmania infantum	74%	99%
E9B181	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	99%
O14214	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P0CS10	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	29%	89%
P0CS11	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	28%	89%
Q08DP1	Bos taurus	27%	100%
Q4I8X0	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	26%	94%
Q4Q6Y3	Leishmania major	75%	100%
Q4WXA1	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	96%
Q59Q39	Candida albicans (strain SC5314 / ATCC MYA-2876)	25%	100%
Q5B8X0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	28%	92%
Q5RJK3	Rattus norvegicus	26%	100%
Q6FQB2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q6PF06	Homo sapiens	25%	100%
Q75A17	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	28%	100%
Q9D075	Mus musculus	25%	100%
V5DNZ8	Trypanosoma cruzi	50%	100%