GRAM domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HIK4_LEIBR

TriTrypDb:

LbrM.30.2680 , LBRM2903_300033300 , LBRM2903_300033400

Length:

709

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0016020	membrane	2	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0110165	cellular anatomical entity	1	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

A4HIK4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIK4

PDB structure(s): 4tyz_A , 4tyz_B

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	499	503	PF00656	0.492
CLV_C14_Caspase3-7	518	522	PF00656	0.439
CLV_C14_Caspase3-7	616	620	PF00656	0.357
CLV_NRD_NRD_1	168	170	PF00675	0.679
CLV_NRD_NRD_1	181	183	PF00675	0.562
CLV_NRD_NRD_1	204	206	PF00675	0.693
CLV_NRD_NRD_1	263	265	PF00675	0.527
CLV_NRD_NRD_1	413	415	PF00675	0.440
CLV_NRD_NRD_1	606	608	PF00675	0.416
CLV_PCSK_KEX2_1	168	170	PF00082	0.684
CLV_PCSK_KEX2_1	263	265	PF00082	0.524
CLV_PCSK_KEX2_1	413	415	PF00082	0.440
CLV_PCSK_KEX2_1	606	608	PF00082	0.326
CLV_PCSK_SKI1_1	134	138	PF00082	0.490
CLV_PCSK_SKI1_1	532	536	PF00082	0.603
CLV_PCSK_SKI1_1	606	610	PF00082	0.416
CLV_PCSK_SKI1_1	668	672	PF00082	0.312
DEG_APCC_KENBOX_2	170	174	PF00400	0.522
DEG_SPOP_SBC_1	142	146	PF00917	0.521
DEG_SPOP_SBC_1	214	218	PF00917	0.586
DOC_CYCLIN_yCln2_LP_2	293	299	PF00134	0.311
DOC_CYCLIN_yCln2_LP_2	573	579	PF00134	0.589
DOC_MAPK_DCC_7	266	276	PF00069	0.513
DOC_MAPK_gen_1	263	273	PF00069	0.522
DOC_MAPK_gen_1	4	11	PF00069	0.462
DOC_MAPK_MEF2A_6	267	276	PF00069	0.510
DOC_PP2B_LxvP_1	573	576	PF13499	0.588
DOC_PP4_FxxP_1	380	383	PF00568	0.392
DOC_PP4_FxxP_1	538	541	PF00568	0.637
DOC_USP7_MATH_1	142	146	PF00917	0.558
DOC_USP7_MATH_1	158	162	PF00917	0.667
DOC_USP7_MATH_1	214	218	PF00917	0.668
DOC_USP7_MATH_1	252	256	PF00917	0.582
DOC_USP7_MATH_1	34	38	PF00917	0.538
DOC_USP7_MATH_1	507	511	PF00917	0.524
DOC_USP7_MATH_1	522	526	PF00917	0.497
DOC_USP7_MATH_1	550	554	PF00917	0.480
DOC_USP7_MATH_1	56	60	PF00917	0.582
DOC_USP7_MATH_1	701	705	PF00917	0.416
DOC_USP7_MATH_2	285	291	PF00917	0.387
DOC_USP7_UBL2_3	202	206	PF12436	0.609
DOC_USP7_UBL2_3	662	666	PF12436	0.357
DOC_WW_Pin1_4	219	224	PF00397	0.633
DOC_WW_Pin1_4	379	384	PF00397	0.426
LIG_14-3-3_CanoR_1	27	36	PF00244	0.507
LIG_14-3-3_CanoR_1	413	419	PF00244	0.391
LIG_14-3-3_CanoR_1	532	542	PF00244	0.516
LIG_Actin_WH2_2	26	43	PF00022	0.485
LIG_AP2alpha_2	378	380	PF02296	0.466
LIG_BIR_II_1	1	5	PF00653	0.623
LIG_BRCT_BRCA1_1	112	116	PF00533	0.317
LIG_CtBP_PxDLS_1	285	289	PF00389	0.403
LIG_deltaCOP1_diTrp_1	120	130	PF00928	0.352
LIG_eIF4E_1	416	422	PF01652	0.356
LIG_FHA_1	214	220	PF00498	0.663
LIG_FHA_1	24	30	PF00498	0.457
LIG_FHA_1	300	306	PF00498	0.504
LIG_FHA_1	333	339	PF00498	0.377
LIG_FHA_1	429	435	PF00498	0.445
LIG_FHA_1	462	468	PF00498	0.468
LIG_FHA_1	49	55	PF00498	0.533
LIG_FHA_1	592	598	PF00498	0.545
LIG_FHA_1	684	690	PF00498	0.332
LIG_FHA_2	208	214	PF00498	0.650
LIG_FHA_2	243	249	PF00498	0.555
LIG_FHA_2	302	308	PF00498	0.462
LIG_FHA_2	326	332	PF00498	0.444
LIG_FHA_2	359	365	PF00498	0.475
LIG_FHA_2	510	516	PF00498	0.434
LIG_FHA_2	614	620	PF00498	0.394
LIG_LIR_Apic_2	326	332	PF02991	0.526
LIG_LIR_Apic_2	378	383	PF02991	0.403
LIG_LIR_Apic_2	535	541	PF02991	0.642
LIG_LIR_Gen_1	295	305	PF02991	0.405
LIG_LIR_Gen_1	335	343	PF02991	0.350
LIG_LIR_Gen_1	417	425	PF02991	0.380
LIG_LIR_Gen_1	459	467	PF02991	0.564
LIG_LIR_Gen_1	639	650	PF02991	0.314
LIG_LIR_Gen_1	655	665	PF02991	0.227
LIG_LIR_Gen_1	696	707	PF02991	0.298
LIG_LIR_Nem_3	295	301	PF02991	0.396
LIG_LIR_Nem_3	335	340	PF02991	0.349
LIG_LIR_Nem_3	345	351	PF02991	0.320
LIG_LIR_Nem_3	417	422	PF02991	0.354
LIG_LIR_Nem_3	459	463	PF02991	0.560
LIG_LIR_Nem_3	557	561	PF02991	0.497
LIG_LIR_Nem_3	639	645	PF02991	0.314
LIG_LIR_Nem_3	655	661	PF02991	0.298
LIG_LIR_Nem_3	696	702	PF02991	0.357
LIG_LRP6_Inhibitor_1	350	356	PF00058	0.262
LIG_MLH1_MIPbox_1	112	116	PF16413	0.317
LIG_PCNA_yPIPBox_3	168	180	PF02747	0.519
LIG_PCNA_yPIPBox_3	330	342	PF02747	0.444
LIG_Pex14_2	104	108	PF04695	0.465
LIG_Pex14_2	605	609	PF04695	0.392
LIG_Pex14_2	621	625	PF04695	0.298
LIG_Pex14_2	688	692	PF04695	0.298
LIG_REV1ctd_RIR_1	113	121	PF16727	0.330
LIG_REV1ctd_RIR_1	135	141	PF16727	0.516
LIG_RPA_C_Fungi	200	212	PF08784	0.517
LIG_SH2_CRK	419	423	PF00017	0.322
LIG_SH2_CRK	699	703	PF00017	0.346
LIG_SH2_NCK_1	419	423	PF00017	0.405
LIG_SH2_PTP2	337	340	PF00017	0.341
LIG_SH2_SRC	658	661	PF00017	0.357
LIG_SH2_STAP1	416	420	PF00017	0.359
LIG_SH2_STAP1	699	703	PF00017	0.287
LIG_SH2_STAT5	298	301	PF00017	0.395
LIG_SH2_STAT5	337	340	PF00017	0.341
LIG_SH2_STAT5	348	351	PF00017	0.328
LIG_SH2_STAT5	354	357	PF00017	0.380
LIG_SH2_STAT5	416	419	PF00017	0.398
LIG_SH2_STAT5	635	638	PF00017	0.368
LIG_SH3_2	262	267	PF14604	0.689
LIG_SH3_3	259	265	PF00018	0.682
LIG_SH3_3	269	275	PF00018	0.560
LIG_SH3_3	386	392	PF00018	0.334
LIG_SH3_3	419	425	PF00018	0.329
LIG_SH3_3	471	477	PF00018	0.664
LIG_SH3_3	495	501	PF00018	0.446
LIG_SUMO_SIM_anti_2	30	37	PF11976	0.495
LIG_SUMO_SIM_anti_2	97	102	PF11976	0.344
LIG_SUMO_SIM_par_1	449	455	PF11976	0.467
LIG_SUMO_SIM_par_1	586	591	PF11976	0.440
LIG_TRAF2_1	304	307	PF00917	0.310
LIG_TRAF2_1	443	446	PF00917	0.302
LIG_TRAF2_1	528	531	PF00917	0.641
LIG_TYR_ITIM	656	661	PF00017	0.371
LIG_TYR_ITSM	415	422	PF00017	0.415
LIG_Vh1_VBS_1	54	72	PF01044	0.317
LIG_WRC_WIRS_1	105	110	PF05994	0.476
LIG_WRC_WIRS_1	324	329	PF05994	0.457
LIG_WRC_WIRS_1	484	489	PF05994	0.432
LIG_WRC_WIRS_1	618	623	PF05994	0.357
MOD_CK1_1	145	151	PF00069	0.522
MOD_CK1_1	157	163	PF00069	0.499
MOD_CK1_1	196	202	PF00069	0.740
MOD_CK1_1	2	8	PF00069	0.530
MOD_CK1_1	217	223	PF00069	0.636
MOD_CK1_1	23	29	PF00069	0.427
MOD_CK1_1	232	238	PF00069	0.695
MOD_CK1_1	322	328	PF00069	0.493
MOD_CK1_1	358	364	PF00069	0.592
MOD_CK1_1	393	399	PF00069	0.376
MOD_CK1_1	59	65	PF00069	0.502
MOD_CK2_1	207	213	PF00069	0.703
MOD_CK2_1	300	306	PF00069	0.410
MOD_CK2_1	325	331	PF00069	0.451
MOD_CK2_1	500	506	PF00069	0.483
MOD_CK2_1	509	515	PF00069	0.610
MOD_CK2_1	78	84	PF00069	0.436
MOD_Cter_Amidation	180	183	PF01082	0.581
MOD_GlcNHglycan	147	150	PF01048	0.518
MOD_GlcNHglycan	155	159	PF01048	0.463
MOD_GlcNHglycan	160	163	PF01048	0.465
MOD_GlcNHglycan	195	198	PF01048	0.794
MOD_GlcNHglycan	22	25	PF01048	0.468
MOD_GlcNHglycan	240	243	PF01048	0.648
MOD_GlcNHglycan	254	257	PF01048	0.664
MOD_GlcNHglycan	489	492	PF01048	0.557
MOD_GlcNHglycan	517	520	PF01048	0.450
MOD_GlcNHglycan	552	555	PF01048	0.519
MOD_GSK3_1	141	148	PF00069	0.582
MOD_GSK3_1	154	161	PF00069	0.608
MOD_GSK3_1	213	220	PF00069	0.642
MOD_GSK3_1	23	30	PF00069	0.414
MOD_GSK3_1	234	241	PF00069	0.735
MOD_GSK3_1	252	259	PF00069	0.617
MOD_GSK3_1	287	294	PF00069	0.437
MOD_GSK3_1	319	326	PF00069	0.433
MOD_GSK3_1	390	397	PF00069	0.375
MOD_GSK3_1	483	490	PF00069	0.459
MOD_GSK3_1	550	557	PF00069	0.476
MOD_GSK3_1	56	63	PF00069	0.466
MOD_GSK3_1	613	620	PF00069	0.381
MOD_GSK3_1	683	690	PF00069	0.319
MOD_LATS_1	25	31	PF00433	0.488
MOD_N-GLC_1	20	25	PF02516	0.417
MOD_N-GLC_1	322	327	PF02516	0.375
MOD_N-GLC_1	683	688	PF02516	0.357
MOD_N-GLC_2	71	73	PF02516	0.298
MOD_NEK2_1	104	109	PF00069	0.470
MOD_NEK2_1	130	135	PF00069	0.481
MOD_NEK2_1	299	304	PF00069	0.454
MOD_NEK2_1	487	492	PF00069	0.437
MOD_NEK2_1	588	593	PF00069	0.521
MOD_NEK2_1	61	66	PF00069	0.362
MOD_NEK2_1	626	631	PF00069	0.318
MOD_NEK2_1	636	641	PF00069	0.380
MOD_NEK2_1	645	650	PF00069	0.400
MOD_NEK2_1	683	688	PF00069	0.327
MOD_NEK2_2	522	527	PF00069	0.486
MOD_OFUCOSY	69	75	PF10250	0.473
MOD_PIKK_1	107	113	PF00454	0.464
MOD_PIKK_1	182	188	PF00454	0.514
MOD_PIKK_1	222	228	PF00454	0.679
MOD_PIKK_1	229	235	PF00454	0.581
MOD_PIKK_1	34	40	PF00454	0.541
MOD_PIKK_1	358	364	PF00454	0.483
MOD_PIKK_1	420	426	PF00454	0.315
MOD_PIKK_1	56	62	PF00454	0.568
MOD_PIKK_1	701	707	PF00454	0.371
MOD_PK_1	666	672	PF00069	0.371
MOD_PKA_1	182	188	PF00069	0.514
MOD_PKA_1	206	212	PF00069	0.665
MOD_PKA_1	267	273	PF00069	0.517
MOD_PKA_2	238	244	PF00069	0.598
MOD_PKA_2	355	361	PF00069	0.332
MOD_PKA_2	40	46	PF00069	0.384
MOD_PKA_2	461	467	PF00069	0.447
MOD_Plk_1	154	160	PF00069	0.455
MOD_Plk_1	214	220	PF00069	0.653
MOD_Plk_1	242	248	PF00069	0.573
MOD_Plk_1	286	292	PF00069	0.379
MOD_Plk_1	322	328	PF00069	0.384
MOD_Plk_1	393	399	PF00069	0.464
MOD_Plk_1	522	528	PF00069	0.518
MOD_Plk_1	626	632	PF00069	0.333
MOD_Plk_1	683	689	PF00069	0.398
MOD_Plk_2-3	78	84	PF00069	0.374
MOD_Plk_4	11	17	PF00069	0.335
MOD_Plk_4	110	116	PF00069	0.397
MOD_Plk_4	242	248	PF00069	0.424
MOD_Plk_4	332	338	PF00069	0.406
MOD_Plk_4	414	420	PF00069	0.363
MOD_Plk_4	522	528	PF00069	0.562
MOD_Plk_4	613	619	PF00069	0.341
MOD_Plk_4	683	689	PF00069	0.307
MOD_Plk_4	85	91	PF00069	0.623
MOD_ProDKin_1	219	225	PF00069	0.635
MOD_ProDKin_1	379	385	PF00069	0.427
MOD_SUMO_rev_2	185	195	PF00179	0.513
MOD_SUMO_rev_2	655	664	PF00179	0.357
TRG_DiLeu_BaEn_1	190	195	PF01217	0.518
TRG_DiLeu_BaEn_1	295	300	PF01217	0.396
TRG_DiLeu_BaEn_1	97	102	PF01217	0.438
TRG_DiLeu_BaEn_3	445	451	PF01217	0.270
TRG_DiLeu_BaEn_4	286	292	PF01217	0.379
TRG_ENDOCYTIC_2	298	301	PF00928	0.395
TRG_ENDOCYTIC_2	337	340	PF00928	0.341
TRG_ENDOCYTIC_2	419	422	PF00928	0.349
TRG_ENDOCYTIC_2	570	573	PF00928	0.437
TRG_ENDOCYTIC_2	635	638	PF00928	0.368
TRG_ENDOCYTIC_2	658	661	PF00928	0.357
TRG_ENDOCYTIC_2	699	702	PF00928	0.287
TRG_ER_diArg_1	168	170	PF00400	0.625
TRG_ER_diArg_1	262	264	PF00400	0.523
TRG_ER_diArg_1	413	415	PF00400	0.440
TRG_ER_diArg_1	605	607	PF00400	0.317
TRG_NLS_MonoExtC_3	201	206	PF00514	0.668
TRG_NLS_MonoExtN_4	202	209	PF00514	0.594
TRG_NLS_MonoExtN_4	263	270	PF00514	0.526
TRG_Pf-PMV_PEXEL_1	182	186	PF00026	0.516
TRG_Pf-PMV_PEXEL_1	27	31	PF00026	0.491

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I443	Leptomonas seymouri	54%	98%
A0A3R7L2R2	Trypanosoma rangeli	34%	100%
A0A3S7X3S1	Leishmania donovani	69%	97%
A4I5U9	Leishmania infantum	69%	97%
E9B144	Leishmania mexicana (strain MHOM/GT/2001/U1103)	70%	97%
Q4Q722	Leishmania major	69%	100%