RING-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase, putative

Species:

Leishmania braziliensis

UniProt:

A4HIE9_LEIBR

TriTrypDb:

LbrM.30.2120 , LBRM2903_300027400

Length:

518

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	1
GO:0005737	cytoplasm	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A4HIE9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIE9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0009894	regulation of catabolic process	4	1
GO:0009896	positive regulation of catabolic process	5	1
GO:0009987	cellular process	1	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0016567	protein ubiquitination	7	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0030162	regulation of proteolysis	6	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031331	positive regulation of cellular catabolic process	6	1
GO:0032434	regulation of proteasomal ubiquitin-dependent protein catabolic process	7	1
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	8	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0042176	regulation of protein catabolic process	5	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0045732	positive regulation of protein catabolic process	6	1
GO:0045862	positive regulation of proteolysis	7	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061136	regulation of proteasomal protein catabolic process	6	1
GO:0065007	biological regulation	1	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1
GO:1901800	positive regulation of proteasomal protein catabolic process	7	1
GO:1903050	regulation of proteolysis involved in protein catabolic process	7	1
GO:1903052	positive regulation of proteolysis involved in protein catabolic process	8	1
GO:2000058	regulation of ubiquitin-dependent protein catabolic process	6	1
GO:2000060	positive regulation of ubiquitin-dependent protein catabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0004842	ubiquitin-protein transferase activity	4	7
GO:0005488	binding	1	6
GO:0016740	transferase activity	2	7
GO:0019787	ubiquitin-like protein transferase activity	3	7
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6
GO:0140096	catalytic activity, acting on a protein	2	7
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031624	ubiquitin conjugating enzyme binding	5	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0061630	ubiquitin protein ligase activity	5	1
GO:0061659	ubiquitin-like protein ligase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	21	25	PF00656	0.519
CLV_C14_Caspase3-7	355	359	PF00656	0.478
CLV_NRD_NRD_1	211	213	PF00675	0.365
CLV_NRD_NRD_1	81	83	PF00675	0.427
CLV_PCSK_KEX2_1	361	363	PF00082	0.548
CLV_PCSK_KEX2_1	407	409	PF00082	0.462
CLV_PCSK_KEX2_1	81	83	PF00082	0.427
CLV_PCSK_PC1ET2_1	361	363	PF00082	0.432
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.423
CLV_PCSK_SKI1_1	188	192	PF00082	0.289
CLV_PCSK_SKI1_1	213	217	PF00082	0.321
CLV_PCSK_SKI1_1	269	273	PF00082	0.285
CLV_PCSK_SKI1_1	361	365	PF00082	0.562
CLV_PCSK_SKI1_1	440	444	PF00082	0.507
CLV_PCSK_SKI1_1	446	450	PF00082	0.465
CLV_PCSK_SKI1_1	489	493	PF00082	0.451
CLV_PCSK_SKI1_1	99	103	PF00082	0.291
DEG_APCC_DBOX_1	393	401	PF00400	0.349
DEG_Nend_Nbox_1	1	3	PF02207	0.501
DOC_CDC14_PxL_1	239	247	PF14671	0.365
DOC_CYCLIN_RxL_1	185	195	PF00134	0.280
DOC_CYCLIN_RxL_1	437	447	PF00134	0.280
DOC_CYCLIN_yCln2_LP_2	238	244	PF00134	0.208
DOC_MAPK_gen_1	81	91	PF00069	0.430
DOC_MAPK_MEF2A_6	431	438	PF00069	0.311
DOC_MAPK_RevD_3	66	82	PF00069	0.385
DOC_PP2B_LxvP_1	238	241	PF13499	0.208
DOC_USP7_MATH_1	350	354	PF00917	0.562
DOC_USP7_MATH_1	356	360	PF00917	0.437
DOC_USP7_MATH_1	56	60	PF00917	0.451
DOC_USP7_UBL2_3	193	197	PF12436	0.382
DOC_USP7_UBL2_3	305	309	PF12436	0.326
LIG_14-3-3_CanoR_1	262	266	PF00244	0.289
LIG_14-3-3_CanoR_1	269	276	PF00244	0.289
LIG_14-3-3_CanoR_1	277	283	PF00244	0.374
LIG_14-3-3_CanoR_1	362	366	PF00244	0.407
LIG_14-3-3_CanoR_1	369	373	PF00244	0.314
LIG_14-3-3_CanoR_1	437	443	PF00244	0.448
LIG_Actin_WH2_2	246	264	PF00022	0.365
LIG_APCC_ABBAyCdc20_2	369	375	PF00400	0.362
LIG_BIR_III_2	24	28	PF00653	0.496
LIG_BRCT_BRCA1_1	320	324	PF00533	0.208
LIG_BRCT_BRCA1_1	359	363	PF00533	0.415
LIG_BRCT_BRCA1_1	498	502	PF00533	0.293
LIG_deltaCOP1_diTrp_1	323	332	PF00928	0.365
LIG_FHA_1	214	220	PF00498	0.483
LIG_FHA_1	369	375	PF00498	0.389
LIG_FHA_1	437	443	PF00498	0.363
LIG_FHA_1	46	52	PF00498	0.443
LIG_FHA_1	510	516	PF00498	0.374
LIG_FHA_1	75	81	PF00498	0.323
LIG_FHA_2	17	23	PF00498	0.517
LIG_FHA_2	416	422	PF00498	0.407
LIG_FHA_2	442	448	PF00498	0.415
LIG_FHA_2	498	504	PF00498	0.490
LIG_LIR_Apic_2	224	229	PF02991	0.203
LIG_LIR_Gen_1	321	331	PF02991	0.350
LIG_LIR_Gen_1	452	459	PF02991	0.412
LIG_LIR_Nem_3	321	327	PF02991	0.350
LIG_LIR_Nem_3	364	370	PF02991	0.443
LIG_MAD2	402	410	PF02301	0.384
LIG_MLH1_MIPbox_1	498	502	PF16413	0.293
LIG_NRBOX	164	170	PF00104	0.274
LIG_PTB_Apo_2	253	260	PF02174	0.365
LIG_PTB_Phospho_1	253	259	PF10480	0.365
LIG_SH2_CRK	239	243	PF00017	0.365
LIG_SH2_NCK_1	239	243	PF00017	0.365
LIG_SH2_SRC	239	242	PF00017	0.365
LIG_SH2_SRC	265	268	PF00017	0.365
LIG_SH2_STAP1	370	374	PF00017	0.357
LIG_SH2_STAP1	6	10	PF00017	0.591
LIG_SH2_STAT3	20	23	PF00017	0.794
LIG_SH2_STAT5	150	153	PF00017	0.312
LIG_SH2_STAT5	18	21	PF00017	0.520
LIG_SH2_STAT5	214	217	PF00017	0.289
LIG_SH2_STAT5	259	262	PF00017	0.365
LIG_SH2_STAT5	282	285	PF00017	0.444
LIG_SH2_STAT5	292	295	PF00017	0.520
LIG_SH2_STAT5	370	373	PF00017	0.351
LIG_SH2_STAT5	453	456	PF00017	0.325
LIG_SH2_STAT5	458	461	PF00017	0.401
LIG_SH2_STAT5	94	97	PF00017	0.444
LIG_SH3_3	238	244	PF00018	0.294
LIG_SH3_3	264	270	PF00018	0.312
LIG_SUMO_SIM_anti_2	420	428	PF11976	0.384
LIG_TRAF2_1	19	22	PF00917	0.604
LIG_TRAF2_1	352	355	PF00917	0.728
LIG_TRAF2_1	418	421	PF00917	0.360
LIG_TYR_ITIM	237	242	PF00017	0.365
LIG_UBA3_1	206	213	PF00899	0.434
LIG_ULM_U2AF65_1	81	86	PF00076	0.415
LIG_WRC_WIRS_1	498	503	PF05994	0.430
MOD_CK1_1	281	287	PF00069	0.468
MOD_CK1_1	493	499	PF00069	0.329
MOD_CK1_1	74	80	PF00069	0.315
MOD_CK2_1	109	115	PF00069	0.461
MOD_CK2_1	16	22	PF00069	0.516
MOD_CK2_1	169	175	PF00069	0.251
MOD_CK2_1	415	421	PF00069	0.404
MOD_CK2_1	441	447	PF00069	0.326
MOD_GlcNHglycan	308	312	PF01048	0.292
MOD_GlcNHglycan	31	34	PF01048	0.646
MOD_GlcNHglycan	335	338	PF01048	0.475
MOD_GlcNHglycan	375	378	PF01048	0.405
MOD_GlcNHglycan	413	416	PF01048	0.682
MOD_GlcNHglycan	483	486	PF01048	0.393
MOD_GlcNHglycan	58	61	PF01048	0.436
MOD_GSK3_1	105	112	PF00069	0.343
MOD_GSK3_1	25	32	PF00069	0.629
MOD_GSK3_1	303	310	PF00069	0.398
MOD_GSK3_1	314	321	PF00069	0.345
MOD_GSK3_1	357	364	PF00069	0.542
MOD_GSK3_1	411	418	PF00069	0.407
MOD_GSK3_1	425	432	PF00069	0.527
MOD_GSK3_1	45	52	PF00069	0.482
MOD_GSK3_1	477	484	PF00069	0.389
MOD_GSK3_1	489	496	PF00069	0.447
MOD_GSK3_1	66	73	PF00069	0.412
MOD_LATS_1	211	217	PF00433	0.341
MOD_N-GLC_1	66	71	PF02516	0.429
MOD_NEK2_1	168	173	PF00069	0.319
MOD_NEK2_1	218	223	PF00069	0.289
MOD_NEK2_1	254	259	PF00069	0.208
MOD_NEK2_1	294	299	PF00069	0.289
MOD_NEK2_1	357	362	PF00069	0.375
MOD_NEK2_1	383	388	PF00069	0.500
MOD_NEK2_1	424	429	PF00069	0.328
MOD_NEK2_1	436	441	PF00069	0.335
MOD_NEK2_1	449	454	PF00069	0.352
MOD_NEK2_1	483	488	PF00069	0.473
MOD_NEK2_1	490	495	PF00069	0.422
MOD_NEK2_1	71	76	PF00069	0.365
MOD_NEK2_1	95	100	PF00069	0.396
MOD_NEK2_2	143	148	PF00069	0.250
MOD_OFUCOSY	313	318	PF10250	0.289
MOD_PIKK_1	415	421	PF00454	0.371
MOD_PIKK_1	493	499	PF00454	0.482
MOD_PKA_1	361	367	PF00069	0.480
MOD_PKA_2	261	267	PF00069	0.409
MOD_PKA_2	361	367	PF00069	0.429
MOD_PKA_2	368	374	PF00069	0.445
MOD_PKA_2	436	442	PF00069	0.457
MOD_PKA_2	83	89	PF00069	0.513
MOD_Plk_1	109	115	PF00069	0.305
MOD_Plk_1	16	22	PF00069	0.516
MOD_Plk_1	66	72	PF00069	0.423
MOD_Plk_2-3	109	115	PF00069	0.447
MOD_Plk_2-3	16	22	PF00069	0.516
MOD_Plk_2-3	45	51	PF00069	0.477
MOD_Plk_4	221	227	PF00069	0.272
MOD_Plk_4	25	31	PF00069	0.629
MOD_Plk_4	254	260	PF00069	0.208
MOD_Plk_4	383	389	PF00069	0.351
MOD_Plk_4	438	444	PF00069	0.368
MOD_Plk_4	449	455	PF00069	0.311
MOD_Plk_4	483	489	PF00069	0.541
MOD_Plk_4	497	503	PF00069	0.412
MOD_Plk_4	74	80	PF00069	0.309
MOD_SUMO_for_1	430	433	PF00179	0.322
MOD_SUMO_rev_2	189	198	PF00179	0.293
MOD_SUMO_rev_2	375	384	PF00179	0.471
TRG_DiLeu_BaEn_1	420	425	PF01217	0.391
TRG_DiLeu_BaEn_3	201	207	PF01217	0.297
TRG_ENDOCYTIC_2	150	153	PF00928	0.388
TRG_ENDOCYTIC_2	239	242	PF00928	0.365
TRG_ENDOCYTIC_2	453	456	PF00928	0.325
TRG_ER_diArg_1	80	82	PF00400	0.417

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8P3	Leptomonas seymouri	51%	100%
A0A0S4JJP0	Bodo saltans	26%	100%
A0A1X0P1P5	Trypanosomatidae	26%	100%
A0A3Q8IEV4	Leishmania donovani	80%	98%
A0A3R7JXL5	Trypanosoma rangeli	29%	100%
A2VEA3	Bos taurus	26%	93%
A4I5P6	Leishmania infantum	81%	98%
B1H1E4	Xenopus tropicalis	26%	98%
C9ZR47	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9B0Z0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
O01965	Caenorhabditis elegans	25%	100%
O76924	Drosophila melanogaster	24%	100%
O95376	Homo sapiens	27%	100%
P0C8K8	Arabidopsis thaliana	27%	94%
P36113	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	94%
Q22431	Caenorhabditis elegans	23%	100%
Q32NS4	Xenopus laevis	26%	98%
Q6NW85	Danio rerio	26%	97%
Q6PFJ9	Danio rerio	26%	98%
Q6T486	Dictyostelium discoideum	26%	100%
Q84RR0	Arabidopsis thaliana	31%	92%
Q84RR2	Arabidopsis thaliana	25%	87%
Q8L829	Arabidopsis thaliana	30%	94%
Q8W468	Arabidopsis thaliana	26%	91%
Q94981	Drosophila melanogaster	27%	100%
Q949V6	Arabidopsis thaliana	24%	87%
Q9LVW9	Arabidopsis thaliana	27%	98%
Q9LVX0	Arabidopsis thaliana	25%	96%
Q9NE54	Leishmania major	80%	100%
Q9P3U4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9SKC3	Arabidopsis thaliana	27%	95%
Q9SKC4	Arabidopsis thaliana	26%	100%
Q9Y4X5	Homo sapiens	26%	93%
Q9Z1K5	Mus musculus	26%	93%
Q9Z1K6	Mus musculus	27%	100%
V5AQL7	Trypanosoma cruzi	29%	100%