PNPLA domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Domain of unknown function (DUF3336)/Patatin-like phospholipase, putative

Species:

Leishmania braziliensis

UniProt:

A4HIE6_LEIBR

TriTrypDb:

LbrM.30.2090 , LBRM2903_300027100

Length:

764

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	8
GO:0110165	cellular anatomical entity	1	8

Expansion

Sequence features

A4HIE6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIE6

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0009056	catabolic process	2	6
GO:0016042	lipid catabolic process	4	6
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901575	organic substance catabolic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016787	hydrolase activity	2	11
GO:0004806	triglyceride lipase activity	5	8
GO:0016298	lipase activity	4	8
GO:0016788	hydrolase activity, acting on ester bonds	3	8
GO:0052689	carboxylic ester hydrolase activity	4	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	93	97	PF00656	0.508
CLV_MEL_PAP_1	670	676	PF00089	0.382
CLV_NRD_NRD_1	195	197	PF00675	0.440
CLV_NRD_NRD_1	215	217	PF00675	0.419
CLV_NRD_NRD_1	396	398	PF00675	0.361
CLV_NRD_NRD_1	488	490	PF00675	0.414
CLV_NRD_NRD_1	606	608	PF00675	0.253
CLV_PCSK_KEX2_1	215	217	PF00082	0.419
CLV_PCSK_KEX2_1	488	490	PF00082	0.453
CLV_PCSK_KEX2_1	606	608	PF00082	0.253
CLV_PCSK_KEX2_1	69	71	PF00082	0.319
CLV_PCSK_PC1ET2_1	69	71	PF00082	0.319
CLV_PCSK_SKI1_1	197	201	PF00082	0.447
CLV_PCSK_SKI1_1	322	326	PF00082	0.414
CLV_PCSK_SKI1_1	397	401	PF00082	0.393
CLV_PCSK_SKI1_1	405	409	PF00082	0.286
CLV_PCSK_SKI1_1	488	492	PF00082	0.453
CLV_PCSK_SKI1_1	542	546	PF00082	0.453
CLV_PCSK_SKI1_1	606	610	PF00082	0.333
CLV_PCSK_SKI1_1	738	742	PF00082	0.312
DEG_APCC_DBOX_1	605	613	PF00400	0.494
DEG_Nend_UBRbox_3	1	3	PF02207	0.596
DEG_SPOP_SBC_1	128	132	PF00917	0.607
DEG_SPOP_SBC_1	363	367	PF00917	0.566
DOC_MAPK_DCC_7	173	183	PF00069	0.609
DOC_MAPK_gen_1	137	146	PF00069	0.688
DOC_MAPK_gen_1	488	499	PF00069	0.253
DOC_MAPK_gen_1	606	612	PF00069	0.599
DOC_MAPK_gen_1	673	681	PF00069	0.579
DOC_MAPK_MEF2A_6	337	344	PF00069	0.584
DOC_PP2B_LxvP_1	549	552	PF13499	0.211
DOC_PP2B_LxvP_1	631	634	PF13499	0.550
DOC_PP2B_LxvP_1	680	683	PF13499	0.491
DOC_PP4_FxxP_1	630	633	PF00568	0.540
DOC_SPAK_OSR1_1	676	680	PF12202	0.520
DOC_USP7_MATH_1	128	132	PF00917	0.607
DOC_USP7_MATH_1	13	17	PF00917	0.574
DOC_USP7_MATH_1	211	215	PF00917	0.698
DOC_USP7_MATH_1	245	249	PF00917	0.747
DOC_USP7_MATH_1	263	267	PF00917	0.680
DOC_USP7_MATH_1	292	296	PF00917	0.642
DOC_USP7_MATH_1	311	315	PF00917	0.738
DOC_USP7_MATH_1	318	322	PF00917	0.671
DOC_USP7_MATH_1	324	328	PF00917	0.689
DOC_USP7_MATH_1	363	367	PF00917	0.637
DOC_USP7_MATH_1	696	700	PF00917	0.581
DOC_WW_Pin1_4	124	129	PF00397	0.648
DOC_WW_Pin1_4	153	158	PF00397	0.654
DOC_WW_Pin1_4	199	204	PF00397	0.608
DOC_WW_Pin1_4	296	301	PF00397	0.693
DOC_WW_Pin1_4	336	341	PF00397	0.616
DOC_WW_Pin1_4	451	456	PF00397	0.274
DOC_WW_Pin1_4	472	477	PF00397	0.266
LIG_14-3-3_CanoR_1	173	181	PF00244	0.617
LIG_14-3-3_CanoR_1	216	226	PF00244	0.735
LIG_14-3-3_CanoR_1	246	253	PF00244	0.639
LIG_14-3-3_CanoR_1	349	355	PF00244	0.563
LIG_14-3-3_CanoR_1	378	382	PF00244	0.681
LIG_14-3-3_CanoR_1	632	638	PF00244	0.554
LIG_14-3-3_CanoR_1	738	747	PF00244	0.504
LIG_14-3-3_CanoR_1	82	88	PF00244	0.584
LIG_14-3-3_CterR_2	760	764	PF00244	0.533
LIG_Actin_WH2_2	180	198	PF00022	0.611
LIG_APCC_ABBA_1	474	479	PF00400	0.253
LIG_BIR_III_2	29	33	PF00653	0.594
LIG_Clathr_ClatBox_1	301	305	PF01394	0.616
LIG_eIF4E_1	403	409	PF01652	0.559
LIG_FHA_1	130	136	PF00498	0.590
LIG_FHA_1	178	184	PF00498	0.631
LIG_FHA_1	256	262	PF00498	0.618
LIG_FHA_1	404	410	PF00498	0.481
LIG_FHA_1	491	497	PF00498	0.293
LIG_FHA_1	52	58	PF00498	0.556
LIG_FHA_1	728	734	PF00498	0.510
LIG_FHA_1	93	99	PF00498	0.492
LIG_FHA_2	512	518	PF00498	0.351
LIG_FHA_2	7	13	PF00498	0.565
LIG_FHA_2	739	745	PF00498	0.479
LIG_GBD_Chelix_1	739	747	PF00786	0.298
LIG_IBAR_NPY_1	624	626	PF08397	0.507
LIG_LIR_Gen_1	4	15	PF02991	0.571
LIG_LIR_Gen_1	706	714	PF02991	0.555
LIG_LIR_Nem_3	18	23	PF02991	0.586
LIG_LIR_Nem_3	4	10	PF02991	0.580
LIG_LIR_Nem_3	585	589	PF02991	0.453
LIG_LIR_Nem_3	678	684	PF02991	0.602
LIG_LIR_Nem_3	706	710	PF02991	0.559
LIG_LIR_Nem_3	715	721	PF02991	0.540
LIG_LIR_Nem_3	728	734	PF02991	0.510
LIG_LIR_Nem_3	79	84	PF02991	0.534
LIG_MYND_1	629	633	PF01753	0.536
LIG_NRBOX	63	69	PF00104	0.507
LIG_PAM2_1	577	589	PF00658	0.411
LIG_Pex14_2	491	495	PF04695	0.253
LIG_Pex14_2	677	681	PF04695	0.548
LIG_REV1ctd_RIR_1	488	496	PF16727	0.253
LIG_SH2_GRB2like	707	710	PF00017	0.538
LIG_SH2_PTP2	626	629	PF00017	0.511
LIG_SH2_SRC	589	592	PF00017	0.491
LIG_SH2_SRC	617	620	PF00017	0.508
LIG_SH2_SRC	626	629	PF00017	0.481
LIG_SH2_STAP1	7	11	PF00017	0.538
LIG_SH2_STAP1	94	98	PF00017	0.483
LIG_SH2_STAT3	373	376	PF00017	0.626
LIG_SH2_STAT3	759	762	PF00017	0.505
LIG_SH2_STAT5	20	23	PF00017	0.565
LIG_SH2_STAT5	548	551	PF00017	0.291
LIG_SH2_STAT5	589	592	PF00017	0.453
LIG_SH2_STAT5	617	620	PF00017	0.483
LIG_SH2_STAT5	626	629	PF00017	0.578
LIG_SH2_STAT5	707	710	PF00017	0.570
LIG_SH2_STAT5	718	721	PF00017	0.568
LIG_SH2_STAT5	94	97	PF00017	0.501
LIG_SH3_3	294	300	PF00018	0.631
LIG_SH3_3	585	591	PF00018	0.453
LIG_SH3_3	708	714	PF00018	0.534
LIG_SH3_5	714	718	PF00018	0.550
LIG_SUMO_SIM_anti_2	264	269	PF11976	0.598
LIG_SUMO_SIM_par_1	179	185	PF11976	0.584
LIG_TRAF2_1	533	536	PF00917	0.253
LIG_TYR_ITIM	615	620	PF00017	0.476
LIG_UBA3_1	445	453	PF00899	0.253
LIG_WRC_WIRS_1	60	65	PF05994	0.519
LIG_WRC_WIRS_1	7	12	PF05994	0.564
MOD_CDK_SPxxK_3	296	303	PF00069	0.675
MOD_CK1_1	114	120	PF00069	0.653
MOD_CK1_1	131	137	PF00069	0.565
MOD_CK1_1	169	175	PF00069	0.669
MOD_CK1_1	218	224	PF00069	0.660
MOD_CK1_1	228	234	PF00069	0.683
MOD_CK1_1	241	247	PF00069	0.698
MOD_CK1_1	248	254	PF00069	0.628
MOD_CK1_1	295	301	PF00069	0.672
MOD_CK1_1	314	320	PF00069	0.644
MOD_CK1_1	327	333	PF00069	0.721
MOD_CK1_1	351	357	PF00069	0.588
MOD_CK1_1	364	370	PF00069	0.657
MOD_CK1_1	411	417	PF00069	0.290
MOD_CK1_1	441	447	PF00069	0.253
MOD_CK1_1	558	564	PF00069	0.292
MOD_CK1_1	6	12	PF00069	0.566
MOD_CK2_1	451	457	PF00069	0.253
MOD_CK2_1	498	504	PF00069	0.245
MOD_CK2_1	511	517	PF00069	0.348
MOD_CK2_1	530	536	PF00069	0.284
MOD_CK2_1	6	12	PF00069	0.637
MOD_CK2_1	738	744	PF00069	0.484
MOD_CMANNOS	651	654	PF00535	0.345
MOD_GlcNHglycan	113	116	PF01048	0.447
MOD_GlcNHglycan	121	124	PF01048	0.491
MOD_GlcNHglycan	147	151	PF01048	0.403
MOD_GlcNHglycan	174	177	PF01048	0.460
MOD_GlcNHglycan	231	234	PF01048	0.496
MOD_GlcNHglycan	247	250	PF01048	0.443
MOD_GlcNHglycan	292	295	PF01048	0.417
MOD_GlcNHglycan	316	319	PF01048	0.446
MOD_GlcNHglycan	329	332	PF01048	0.565
MOD_GlcNHglycan	387	390	PF01048	0.315
MOD_GlcNHglycan	410	413	PF01048	0.315
MOD_GlcNHglycan	440	443	PF01048	0.510
MOD_GlcNHglycan	479	483	PF01048	0.434
MOD_GlcNHglycan	521	524	PF01048	0.465
MOD_GlcNHglycan	557	560	PF01048	0.395
MOD_GlcNHglycan	644	647	PF01048	0.400
MOD_GlcNHglycan	688	691	PF01048	0.326
MOD_GlcNHglycan	714	717	PF01048	0.333
MOD_GSK3_1	124	131	PF00069	0.729
MOD_GSK3_1	165	172	PF00069	0.740
MOD_GSK3_1	177	184	PF00069	0.634
MOD_GSK3_1	195	202	PF00069	0.650
MOD_GSK3_1	207	214	PF00069	0.659
MOD_GSK3_1	215	222	PF00069	0.714
MOD_GSK3_1	225	232	PF00069	0.700
MOD_GSK3_1	235	242	PF00069	0.720
MOD_GSK3_1	244	251	PF00069	0.637
MOD_GSK3_1	268	275	PF00069	0.613
MOD_GSK3_1	292	299	PF00069	0.698
MOD_GSK3_1	314	321	PF00069	0.666
MOD_GSK3_1	325	332	PF00069	0.658
MOD_GSK3_1	336	343	PF00069	0.675
MOD_GSK3_1	347	354	PF00069	0.589
MOD_GSK3_1	362	369	PF00069	0.662
MOD_GSK3_1	377	384	PF00069	0.618
MOD_GSK3_1	399	406	PF00069	0.501
MOD_GSK3_1	408	415	PF00069	0.295
MOD_GSK3_1	437	444	PF00069	0.294
MOD_GSK3_1	461	468	PF00069	0.283
MOD_GSK3_1	526	533	PF00069	0.253
MOD_GSK3_1	59	66	PF00069	0.522
MOD_GSK3_1	633	640	PF00069	0.575
MOD_GSK3_1	660	667	PF00069	0.523
MOD_GSK3_1	686	693	PF00069	0.566
MOD_GSK3_1	717	724	PF00069	0.519
MOD_N-GLC_1	1	6	PF02516	0.405
MOD_N-GLC_1	228	233	PF02516	0.416
MOD_N-GLC_1	526	531	PF02516	0.453
MOD_NEK2_1	159	164	PF00069	0.653
MOD_NEK2_1	195	200	PF00069	0.697
MOD_NEK2_1	237	242	PF00069	0.694
MOD_NEK2_1	272	277	PF00069	0.596
MOD_NEK2_1	304	309	PF00069	0.630
MOD_NEK2_1	348	353	PF00069	0.598
MOD_NEK2_1	408	413	PF00069	0.407
MOD_NEK2_1	437	442	PF00069	0.281
MOD_NEK2_1	449	454	PF00069	0.310
MOD_NEK2_1	461	466	PF00069	0.370
MOD_NEK2_1	490	495	PF00069	0.276
MOD_NEK2_1	51	56	PF00069	0.589
MOD_NEK2_1	526	531	PF00069	0.253
MOD_NEK2_1	555	560	PF00069	0.387
MOD_NEK2_1	618	623	PF00069	0.477
MOD_NEK2_1	63	68	PF00069	0.462
MOD_NEK2_1	76	81	PF00069	0.568
MOD_PIKK_1	364	370	PF00454	0.626
MOD_PIKK_1	530	536	PF00454	0.253
MOD_PIKK_1	618	624	PF00454	0.512
MOD_PK_1	215	221	PF00069	0.667
MOD_PKA_1	215	221	PF00069	0.622
MOD_PKA_2	172	178	PF00069	0.622
MOD_PKA_2	195	201	PF00069	0.636
MOD_PKA_2	215	221	PF00069	0.683
MOD_PKA_2	245	251	PF00069	0.642
MOD_PKA_2	348	354	PF00069	0.554
MOD_PKA_2	377	383	PF00069	0.682
MOD_PKB_1	736	744	PF00069	0.484
MOD_Plk_1	263	269	PF00069	0.666
MOD_Plk_1	511	517	PF00069	0.289
MOD_Plk_1	526	532	PF00069	0.289
MOD_Plk_1	690	696	PF00069	0.494
MOD_Plk_1	76	82	PF00069	0.557
MOD_Plk_2-3	498	504	PF00069	0.209
MOD_Plk_4	131	137	PF00069	0.582
MOD_Plk_4	195	201	PF00069	0.631
MOD_Plk_4	256	262	PF00069	0.697
MOD_Plk_4	263	269	PF00069	0.648
MOD_Plk_4	441	447	PF00069	0.284
MOD_Plk_4	511	517	PF00069	0.253
MOD_Plk_4	582	588	PF00069	0.432
MOD_Plk_4	59	65	PF00069	0.509
MOD_Plk_4	6	12	PF00069	0.618
MOD_Plk_4	660	666	PF00069	0.657
MOD_Plk_4	696	702	PF00069	0.596
MOD_Plk_4	76	82	PF00069	0.504
MOD_ProDKin_1	124	130	PF00069	0.647
MOD_ProDKin_1	153	159	PF00069	0.656
MOD_ProDKin_1	199	205	PF00069	0.609
MOD_ProDKin_1	296	302	PF00069	0.694
MOD_ProDKin_1	336	342	PF00069	0.611
MOD_ProDKin_1	451	457	PF00069	0.274
MOD_ProDKin_1	472	478	PF00069	0.266
TRG_DiLeu_BaEn_2	456	462	PF01217	0.253
TRG_DiLeu_BaLyEn_6	154	159	PF01217	0.686
TRG_DiLeu_BaLyEn_6	604	609	PF01217	0.453
TRG_ENDOCYTIC_2	20	23	PF00928	0.585
TRG_ENDOCYTIC_2	283	286	PF00928	0.626
TRG_ENDOCYTIC_2	617	620	PF00928	0.471
TRG_ENDOCYTIC_2	7	10	PF00928	0.559
TRG_ENDOCYTIC_2	707	710	PF00928	0.576
TRG_ER_diArg_1	487	489	PF00400	0.209
TRG_ER_diArg_1	605	607	PF00400	0.453
TRG_ER_diArg_1	736	739	PF00400	0.495
TRG_Pf-PMV_PEXEL_1	425	429	PF00026	0.469
TRG_Pf-PMV_PEXEL_1	500	504	PF00026	0.445
TRG_Pf-PMV_PEXEL_1	606	611	PF00026	0.301
TRG_Pf-PMV_PEXEL_1	745	750	PF00026	0.284

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8P1	Leptomonas seymouri	53%	92%
A0A3Q8INR2	Leishmania donovani	28%	100%
A4HB80	Leishmania braziliensis	29%	100%
A4I5P3	Leishmania infantum	78%	86%
A4IAC8	Leishmania infantum	29%	100%
E9B0Y7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
E9B5F1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
Q4Q2I2	Leishmania major	28%	100%
Q4Q779	Leishmania major	77%	100%