tRNA pseudouridine synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridine synthase A-like protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HIA0_LEIBR

TriTrypDb:

LbrM.30.1610 , LBRM2903_300021800

Length:

499

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HIA0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HIA0

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	7
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140101	catalytic activity, acting on a tRNA	4	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	1
GO:0016835	carbon-oxygen lyase activity	3	1
GO:0016836	hydro-lyase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	5	9	PF00656	0.753
CLV_NRD_NRD_1	382	384	PF00675	0.532
CLV_NRD_NRD_1	401	403	PF00675	0.463
CLV_NRD_NRD_1	405	407	PF00675	0.441
CLV_NRD_NRD_1	59	61	PF00675	0.358
CLV_NRD_NRD_1	95	97	PF00675	0.436
CLV_PCSK_KEX2_1	382	384	PF00082	0.532
CLV_PCSK_KEX2_1	401	403	PF00082	0.456
CLV_PCSK_KEX2_1	407	409	PF00082	0.469
CLV_PCSK_KEX2_1	474	476	PF00082	0.394
CLV_PCSK_KEX2_1	95	97	PF00082	0.384
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.525
CLV_PCSK_PC1ET2_1	474	476	PF00082	0.394
CLV_PCSK_SKI1_1	224	228	PF00082	0.312
CLV_PCSK_SKI1_1	27	31	PF00082	0.428
CLV_PCSK_SKI1_1	401	405	PF00082	0.574
CLV_PCSK_SKI1_1	408	412	PF00082	0.540
DEG_APCC_DBOX_1	407	415	PF00400	0.522
DEG_SPOP_SBC_1	18	22	PF00917	0.585
DOC_CDC14_PxL_1	449	457	PF14671	0.317
DOC_MAPK_gen_1	60	66	PF00069	0.295
DOC_MAPK_MEF2A_6	224	231	PF00069	0.278
DOC_MAPK_MEF2A_6	257	264	PF00069	0.259
DOC_PP2B_LxvP_1	270	273	PF13499	0.324
DOC_USP7_MATH_1	129	133	PF00917	0.464
DOC_USP7_MATH_1	19	23	PF00917	0.650
DOC_USP7_MATH_1	339	343	PF00917	0.516
DOC_USP7_MATH_1	426	430	PF00917	0.762
DOC_WW_Pin1_4	292	297	PF00397	0.289
DOC_WW_Pin1_4	432	437	PF00397	0.604
DOC_WW_Pin1_4	441	446	PF00397	0.575
LIG_14-3-3_CanoR_1	110	118	PF00244	0.336
LIG_14-3-3_CanoR_1	130	139	PF00244	0.382
LIG_14-3-3_CanoR_1	173	181	PF00244	0.290
LIG_14-3-3_CanoR_1	224	230	PF00244	0.350
LIG_14-3-3_CanoR_1	257	261	PF00244	0.207
LIG_14-3-3_CanoR_1	333	340	PF00244	0.404
LIG_14-3-3_CanoR_1	368	375	PF00244	0.662
LIG_14-3-3_CanoR_1	441	445	PF00244	0.449
LIG_Actin_WH2_2	26	43	PF00022	0.429
LIG_Actin_WH2_2	82	97	PF00022	0.407
LIG_BIR_III_2	136	140	PF00653	0.599
LIG_BIR_III_4	364	368	PF00653	0.452
LIG_BRCT_BRCA1_1	341	345	PF00533	0.408
LIG_deltaCOP1_diTrp_1	385	394	PF00928	0.414
LIG_FHA_1	155	161	PF00498	0.304
LIG_FHA_1	221	227	PF00498	0.185
LIG_FHA_1	257	263	PF00498	0.173
LIG_FHA_1	28	34	PF00498	0.466
LIG_FHA_1	286	292	PF00498	0.327
LIG_FHA_1	293	299	PF00498	0.319
LIG_FHA_1	326	332	PF00498	0.400
LIG_FHA_1	347	353	PF00498	0.387
LIG_FHA_2	276	282	PF00498	0.386
LIG_FHA_2	388	394	PF00498	0.444
LIG_FHA_2	41	47	PF00498	0.415
LIG_FHA_2	426	432	PF00498	0.723
LIG_FHA_2	460	466	PF00498	0.395
LIG_LIR_Gen_1	145	154	PF02991	0.341
LIG_LIR_Gen_1	171	181	PF02991	0.348
LIG_LIR_Gen_1	242	251	PF02991	0.262
LIG_LIR_Gen_1	301	311	PF02991	0.453
LIG_LIR_Gen_1	314	323	PF02991	0.467
LIG_LIR_Nem_3	171	177	PF02991	0.308
LIG_LIR_Nem_3	180	184	PF02991	0.246
LIG_LIR_Nem_3	205	211	PF02991	0.341
LIG_LIR_Nem_3	242	246	PF02991	0.243
LIG_LIR_Nem_3	250	256	PF02991	0.243
LIG_LIR_Nem_3	301	307	PF02991	0.279
LIG_LIR_Nem_3	314	319	PF02991	0.418
LIG_LIR_Nem_3	342	348	PF02991	0.414
LIG_LIR_Nem_3	53	57	PF02991	0.358
LIG_MYND_1	139	143	PF01753	0.511
LIG_Pex14_1	300	304	PF04695	0.243
LIG_Pex14_2	170	174	PF04695	0.294
LIG_SH2_CRK	253	257	PF00017	0.243
LIG_SH2_PTP2	451	454	PF00017	0.427
LIG_SH2_SRC	243	246	PF00017	0.337
LIG_SH2_STAP1	463	467	PF00017	0.510
LIG_SH2_STAT5	146	149	PF00017	0.295
LIG_SH2_STAT5	184	187	PF00017	0.416
LIG_SH2_STAT5	243	246	PF00017	0.259
LIG_SH2_STAT5	348	351	PF00017	0.456
LIG_SH2_STAT5	413	416	PF00017	0.609
LIG_SH2_STAT5	451	454	PF00017	0.367
LIG_SH3_3	136	142	PF00018	0.524
LIG_SH3_3	284	290	PF00018	0.243
LIG_SH3_3	442	448	PF00018	0.694
LIG_SH3_5	142	146	PF00018	0.366
LIG_SUMO_SIM_par_1	278	284	PF11976	0.382
LIG_SUMO_SIM_par_1	295	301	PF11976	0.198
LIG_TRAF2_1	194	197	PF00917	0.431
LIG_TRAF2_1	233	236	PF00917	0.308
LIG_TRAF2_1	462	465	PF00917	0.473
LIG_TYR_ITIM	241	246	PF00017	0.259
LIG_UBA3_1	279	286	PF00899	0.249
MOD_CK1_1	220	226	PF00069	0.149
MOD_CK1_1	3	9	PF00069	0.726
MOD_CK1_1	397	403	PF00069	0.486
MOD_CK2_1	239	245	PF00069	0.252
MOD_CK2_1	275	281	PF00069	0.386
MOD_CK2_1	40	46	PF00069	0.522
MOD_CK2_1	425	431	PF00069	0.741
MOD_CK2_1	459	465	PF00069	0.380
MOD_DYRK1A_RPxSP_1	441	445	PF00069	0.449
MOD_GlcNHglycan	118	121	PF01048	0.332
MOD_GlcNHglycan	313	316	PF01048	0.245
MOD_GlcNHglycan	335	338	PF01048	0.490
MOD_GlcNHglycan	396	399	PF01048	0.577
MOD_GlcNHglycan	428	431	PF01048	0.640
MOD_GSK3_1	125	132	PF00069	0.313
MOD_GSK3_1	13	20	PF00069	0.686
MOD_GSK3_1	23	30	PF00069	0.655
MOD_GSK3_1	271	278	PF00069	0.407
MOD_GSK3_1	392	399	PF00069	0.513
MOD_GSK3_1	437	444	PF00069	0.636
MOD_N-GLC_1	217	222	PF02516	0.281
MOD_N-GLC_1	8	13	PF02516	0.577
MOD_N-GLC_2	210	212	PF02516	0.243
MOD_NEK2_1	1	6	PF00069	0.735
MOD_NEK2_1	125	130	PF00069	0.358
MOD_NEK2_1	202	207	PF00069	0.337
MOD_NEK2_1	222	227	PF00069	0.112
MOD_NEK2_1	244	249	PF00069	0.161
MOD_NEK2_1	311	316	PF00069	0.371
MOD_NEK2_1	40	45	PF00069	0.666
MOD_NEK2_1	425	430	PF00069	0.652
MOD_PIKK_1	339	345	PF00454	0.419
MOD_PKA_2	129	135	PF00069	0.354
MOD_PKA_2	172	178	PF00069	0.297
MOD_PKA_2	256	262	PF00069	0.200
MOD_PKA_2	332	338	PF00069	0.387
MOD_PKA_2	40	46	PF00069	0.453
MOD_PKA_2	440	446	PF00069	0.605
MOD_PKA_2	456	462	PF00069	0.381
MOD_Plk_1	459	465	PF00069	0.392
MOD_Plk_1	82	88	PF00069	0.460
MOD_Plk_2-3	239	245	PF00069	0.243
MOD_Plk_4	145	151	PF00069	0.376
MOD_Plk_4	247	253	PF00069	0.291
MOD_Plk_4	271	277	PF00069	0.344
MOD_ProDKin_1	292	298	PF00069	0.289
MOD_ProDKin_1	432	438	PF00069	0.604
MOD_ProDKin_1	441	447	PF00069	0.566
MOD_SUMO_rev_2	281	288	PF00179	0.248
MOD_SUMO_rev_2	429	435	PF00179	0.629
MOD_SUMO_rev_2	466	476	PF00179	0.395
MOD_SUMO_rev_2	479	486	PF00179	0.446
TRG_DiLeu_BaEn_1	236	241	PF01217	0.248
TRG_DiLeu_BaEn_1	261	266	PF01217	0.259
TRG_DiLeu_BaEn_4	465	471	PF01217	0.395
TRG_DiLeu_BaLyEn_6	293	298	PF01217	0.342
TRG_DiLeu_BaLyEn_6	450	455	PF01217	0.544
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.327
TRG_ENDOCYTIC_2	146	149	PF00928	0.348
TRG_ENDOCYTIC_2	243	246	PF00928	0.251
TRG_ENDOCYTIC_2	253	256	PF00928	0.231
TRG_ENDOCYTIC_2	451	454	PF00928	0.367
TRG_ER_diArg_1	381	383	PF00400	0.544
TRG_ER_diArg_1	401	403	PF00400	0.374
TRG_ER_diArg_1	475	478	PF00400	0.394
TRG_ER_diArg_1	94	96	PF00400	0.337
TRG_NLS_MonoExtC_3	473	479	PF00514	0.387
TRG_NLS_MonoExtN_4	473	478	PF00514	0.395
TRG_Pf-PMV_PEXEL_1	468	472	PF00026	0.306
TRG_Pf-PMV_PEXEL_1	98	102	PF00026	0.396

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA61	Leptomonas seymouri	72%	97%
A0A0S4JB03	Bodo saltans	50%	100%
A0A1X0P1U9	Trypanosomatidae	57%	100%
A0A3S7X3E8	Leishmania donovani	82%	100%
A0A422P3K1	Trypanosoma rangeli	58%	100%
A4I5I4	Leishmania infantum	82%	100%
C9ZQW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9B0S9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q09524	Caenorhabditis elegans	33%	100%
Q4Q7E9	Leishmania major	83%	100%
V5DQN1	Trypanosoma cruzi	59%	95%