p1/s1 nuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

p1/s1 nuclease

Gene product:

p1/s1 nuclease

Species:

Leishmania braziliensis

UniProt:

A4HI96_LEIBR

TriTrypDb:

LbrM.30.1560 , LBRM2903_300021400

Length:

328

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 23, no: 6
NetGPI	no	yes: 0, no: 29

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0009986	cell surface	2	1

Expansion

Sequence features

A4HI96

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HI96

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	30
GO:0006259	DNA metabolic process	4	30
GO:0006308	DNA catabolic process	5	30
GO:0006725	cellular aromatic compound metabolic process	3	30
GO:0006807	nitrogen compound metabolic process	2	30
GO:0008152	metabolic process	1	30
GO:0009056	catabolic process	2	30
GO:0009057	macromolecule catabolic process	4	30
GO:0009987	cellular process	1	30
GO:0019439	aromatic compound catabolic process	4	30
GO:0034641	cellular nitrogen compound metabolic process	3	30
GO:0034655	nucleobase-containing compound catabolic process	4	30
GO:0043170	macromolecule metabolic process	3	30
GO:0044237	cellular metabolic process	2	30
GO:0044238	primary metabolic process	2	30
GO:0044248	cellular catabolic process	3	30
GO:0044260	obsolete cellular macromolecule metabolic process	3	30
GO:0044265	obsolete cellular macromolecule catabolic process	4	30
GO:0044270	cellular nitrogen compound catabolic process	4	30
GO:0046483	heterocycle metabolic process	3	30
GO:0046700	heterocycle catabolic process	4	30
GO:0071704	organic substance metabolic process	2	30
GO:0090304	nucleic acid metabolic process	4	30
GO:1901360	organic cyclic compound metabolic process	3	30
GO:1901361	organic cyclic compound catabolic process	4	30
GO:1901575	organic substance catabolic process	3	30
GO:0006163	purine nucleotide metabolic process	5	1
GO:0006195	purine nucleotide catabolic process	6	1
GO:0006196	AMP catabolic process	7	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009123	nucleoside monophosphate metabolic process	5	1
GO:0009125	nucleoside monophosphate catabolic process	6	1
GO:0009126	purine nucleoside monophosphate metabolic process	6	1
GO:0009128	purine nucleoside monophosphate catabolic process	7	1
GO:0009150	purine ribonucleotide metabolic process	6	1
GO:0009154	purine ribonucleotide catabolic process	6	1
GO:0009158	ribonucleoside monophosphate catabolic process	7	1
GO:0009161	ribonucleoside monophosphate metabolic process	6	1
GO:0009166	nucleotide catabolic process	6	1
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	1
GO:0009169	purine ribonucleoside monophosphate catabolic process	8	1
GO:0009259	ribonucleotide metabolic process	5	1
GO:0009261	ribonucleotide catabolic process	5	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019693	ribose phosphate metabolic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046033	AMP metabolic process	7	1
GO:0046434	organophosphate catabolic process	4	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:0072523	purine-containing compound catabolic process	5	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901136	carbohydrate derivative catabolic process	4	1
GO:1901292	nucleoside phosphate catabolic process	5	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	30
GO:0003824	catalytic activity	1	30
GO:0004518	nuclease activity	4	30
GO:0004519	endonuclease activity	5	30
GO:0005488	binding	1	30
GO:0016787	hydrolase activity	2	30
GO:0016788	hydrolase activity, acting on ester bonds	3	30
GO:0043167	ion binding	2	30
GO:0043169	cation binding	3	30
GO:0046872	metal ion binding	4	30
GO:0097159	organic cyclic compound binding	2	30
GO:1901363	heterocyclic compound binding	2	30
GO:0008252	nucleotidase activity	6	1
GO:0008254	3'-nucleotidase activity	7	1
GO:0016791	phosphatase activity	5	1
GO:0042578	phosphoric ester hydrolase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	204	206	PF00675	0.393
CLV_NRD_NRD_1	44	46	PF00675	0.438
CLV_PCSK_KEX2_1	204	206	PF00082	0.435
CLV_PCSK_KEX2_1	313	315	PF00082	0.739
CLV_PCSK_KEX2_1	44	46	PF00082	0.442
CLV_PCSK_KEX2_1	85	87	PF00082	0.468
CLV_PCSK_PC1ET2_1	313	315	PF00082	0.571
CLV_PCSK_PC1ET2_1	85	87	PF00082	0.468
CLV_PCSK_SKI1_1	14	18	PF00082	0.301
CLV_PCSK_SKI1_1	221	225	PF00082	0.552
CLV_PCSK_SKI1_1	314	318	PF00082	0.609
DEG_APCC_DBOX_1	13	21	PF00400	0.230
DEG_APCC_DBOX_1	132	140	PF00400	0.154
DEG_SPOP_SBC_1	206	210	PF00917	0.226
DOC_CYCLIN_RxL_1	8	21	PF00134	0.384
DOC_CYCLIN_yCln2_LP_2	153	159	PF00134	0.191
DOC_MAPK_gen_1	12	20	PF00069	0.384
DOC_MAPK_MEF2A_6	12	20	PF00069	0.267
DOC_MAPK_MEF2A_6	172	181	PF00069	0.243
DOC_USP7_MATH_1	206	210	PF00917	0.178
DOC_USP7_MATH_1	242	246	PF00917	0.239
DOC_USP7_MATH_1	249	253	PF00917	0.260
DOC_USP7_MATH_1	3	7	PF00917	0.495
DOC_WW_Pin1_4	131	136	PF00397	0.233
DOC_WW_Pin1_4	196	201	PF00397	0.215
DOC_WW_Pin1_4	316	321	PF00397	0.329
DOC_WW_Pin1_4	68	73	PF00397	0.311
LIG_14-3-3_CanoR_1	162	168	PF00244	0.386
LIG_14-3-3_CanoR_1	204	213	PF00244	0.267
LIG_14-3-3_CanoR_1	221	227	PF00244	0.194
LIG_14-3-3_CanoR_1	278	282	PF00244	0.278
LIG_BRCT_BRCA1_1	244	248	PF00533	0.330
LIG_BRCT_BRCA1_1	64	68	PF00533	0.206
LIG_eIF4E_1	288	294	PF01652	0.332
LIG_FHA_1	108	114	PF00498	0.316
LIG_FHA_1	181	187	PF00498	0.273
LIG_FHA_1	197	203	PF00498	0.286
LIG_FHA_1	208	214	PF00498	0.171
LIG_FHA_1	317	323	PF00498	0.507
LIG_FHA_2	232	238	PF00498	0.363
LIG_LIR_Gen_1	252	259	PF02991	0.158
LIG_LIR_Nem_3	93	98	PF02991	0.303
LIG_NRBOX	289	295	PF00104	0.288
LIG_PCNA_PIPBox_1	185	194	PF02747	0.226
LIG_PDZ_Class_1	323	328	PF00595	0.361
LIG_PTB_Apo_2	240	247	PF02174	0.287
LIG_PTB_Phospho_1	240	246	PF10480	0.287
LIG_SH2_CRK	203	207	PF00017	0.290
LIG_SH2_STAP1	163	167	PF00017	0.330
LIG_SH2_STAP1	246	250	PF00017	0.244
LIG_SH2_STAP1	254	258	PF00017	0.221
LIG_SH2_STAP1	307	311	PF00017	0.280
LIG_SH2_STAP1	98	102	PF00017	0.183
LIG_SH2_STAT3	98	101	PF00017	0.296
LIG_SH2_STAT5	145	148	PF00017	0.218
LIG_SH2_STAT5	15	18	PF00017	0.190
LIG_SH2_STAT5	267	270	PF00017	0.211
LIG_SH2_STAT5	288	291	PF00017	0.328
LIG_SH3_3	129	135	PF00018	0.165
LIG_SUMO_SIM_par_1	15	21	PF11976	0.357
LIG_SUMO_SIM_par_1	208	218	PF11976	0.158
LIG_TRFH_1	68	72	PF08558	0.277
LIG_TYR_ITIM	13	18	PF00017	0.163
MOD_CDK_SPxxK_3	316	323	PF00069	0.412
MOD_CK1_1	161	167	PF00069	0.296
MOD_CK1_1	227	233	PF00069	0.213
MOD_CK1_1	277	283	PF00069	0.389
MOD_GlcNHglycan	20	23	PF01048	0.458
MOD_GlcNHglycan	263	266	PF01048	0.202
MOD_GlcNHglycan	307	310	PF01048	0.428
MOD_GlcNHglycan	64	67	PF01048	0.274
MOD_GSK3_1	127	134	PF00069	0.367
MOD_GSK3_1	222	229	PF00069	0.300
MOD_N-GLC_1	107	112	PF02516	0.310
MOD_N-GLC_1	242	247	PF02516	0.305
MOD_N-GLC_1	250	255	PF02516	0.353
MOD_N-GLC_2	194	196	PF02516	0.258
MOD_NEK2_1	125	130	PF00069	0.394
MOD_NEK2_1	222	227	PF00069	0.328
MOD_NEK2_1	62	67	PF00069	0.248
MOD_NEK2_2	3	8	PF00069	0.363
MOD_PKA_2	161	167	PF00069	0.258
MOD_PKA_2	277	283	PF00069	0.361
MOD_Plk_1	242	248	PF00069	0.327
MOD_Plk_1	250	256	PF00069	0.340
MOD_Plk_2-3	231	237	PF00069	0.201
MOD_ProDKin_1	131	137	PF00069	0.268
MOD_ProDKin_1	196	202	PF00069	0.244
MOD_ProDKin_1	316	322	PF00069	0.407
MOD_ProDKin_1	68	74	PF00069	0.377
MOD_SUMO_rev_2	227	235	PF00179	0.186
MOD_SUMO_rev_2	48	55	PF00179	0.173
TRG_DiLeu_BaEn_2	63	69	PF01217	0.288
TRG_DiLeu_BaLyEn_6	218	223	PF01217	0.258
TRG_ENDOCYTIC_2	15	18	PF00928	0.190
TRG_ENDOCYTIC_2	254	257	PF00928	0.283
TRG_ENDOCYTIC_2	97	100	PF00928	0.246
TRG_ER_diArg_1	11	14	PF00400	0.202
TRG_ER_diArg_1	203	205	PF00400	0.213
TRG_PTS1	325	328	PF00515	0.452

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Q7	Leptomonas seymouri	56%	91%
A0A0S4JAL3	Bodo saltans	34%	97%
A0A0S4JML7	Bodo saltans	31%	90%
A0A1X0P2K7	Trypanosomatidae	41%	99%
A0A3Q8IBF6	Leishmania donovani	37%	86%
A0A3Q8IEW3	Leishmania donovani	64%	100%
A0A3Q8IFH4	Leishmania donovani	34%	87%
A0A3Q8IK90	Leishmania donovani	33%	94%
A0A3S7X3A6	Leishmania donovani	65%	100%
A0A422N7B0	Trypanosoma rangeli	40%	99%
A4H6V5	Leishmania braziliensis	36%	100%
A4HJL3	Leishmania braziliensis	35%	100%
A4I5I0	Leishmania infantum	64%	100%
A4I736	Leishmania infantum	34%	87%
C9ZQH5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
C9ZQH6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
C9ZQW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AGC7	Leishmania infantum	37%	86%
E9ANW7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	86%
E9B0S4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	64%	100%
E9B0S5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	66%	100%
E9B231	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	87%
F4JJL0	Arabidopsis thaliana	28%	100%
F4JJL3	Arabidopsis thaliana	25%	100%
P24021	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	27%	100%
Q4Q630	Leishmania major	34%	100%
Q4Q7F3	Leishmania major	63%	100%
Q4Q7F4	Leishmania major	63%	100%
Q4QGQ3	Leishmania major	36%	100%
Q66VY6	Leishmania major	33%	100%
Q8LDW6	Arabidopsis thaliana	27%	100%
Q9C9G4	Arabidopsis thaliana	29%	100%
Q9SXA6	Arabidopsis thaliana	24%	100%
V5B8Z9	Trypanosoma cruzi	41%	85%