Putative ama1 protein

Quick info Annotations Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ama1 protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HI93_LEIBR

TriTrypDb:

LbrM.30.1530 , LBRM2903_300021000

Length:

204

Annotations

LeishMANIAdb annotations

Homologous to the obscure animal PLAC8 protein. These proteins likely immerse into the cell membrane, but without crossing it. Might be involved in heavy metal extrusion.. Might carry exosomal signals on its N-terminus.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HI93

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HI93

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	112	114	PF00675	0.595
CLV_NRD_NRD_1	145	147	PF00675	0.416
CLV_NRD_NRD_1	201	203	PF00675	0.446
CLV_PCSK_KEX2_1	145	147	PF00082	0.416
CLV_PCSK_KEX2_1	201	203	PF00082	0.446
DOC_CKS1_1	167	172	PF01111	0.451
DOC_PP1_RVXF_1	112	119	PF00149	0.399
DOC_PP2B_LxvP_1	8	11	PF13499	0.483
DOC_WW_Pin1_4	166	171	PF00397	0.451
LIG_BRCT_BRCA1_1	136	140	PF00533	0.424
LIG_FHA_1	128	134	PF00498	0.480
LIG_FHA_1	171	177	PF00498	0.529
LIG_FHA_2	123	129	PF00498	0.443
LIG_FHA_2	156	162	PF00498	0.628
LIG_FHA_2	188	194	PF00498	0.739
LIG_SH2_CRK	120	124	PF00017	0.447
LIG_SH2_NCK_1	47	51	PF00017	0.497
LIG_SH2_STAP1	47	51	PF00017	0.400
LIG_SH2_STAT5	120	123	PF00017	0.431
LIG_SH3_3	59	65	PF00018	0.485
LIG_WRC_WIRS_1	123	128	PF05994	0.300
MOD_CK1_1	155	161	PF00069	0.532
MOD_CK2_1	154	160	PF00069	0.657
MOD_CK2_1	187	193	PF00069	0.705
MOD_CK2_1	87	93	PF00069	0.367
MOD_Cter_Amidation	199	202	PF01082	0.434
MOD_GlcNHglycan	154	157	PF01048	0.416
MOD_GlcNHglycan	30	33	PF01048	0.705
MOD_GlcNHglycan	48	51	PF01048	0.626
MOD_GSK3_1	166	173	PF00069	0.480
MOD_GSK3_1	87	94	PF00069	0.315
MOD_N-GLC_1	134	139	PF02516	0.276
MOD_NEK2_1	122	127	PF00069	0.508
MOD_NEK2_1	134	139	PF00069	0.365
MOD_NEK2_1	178	183	PF00069	0.627
MOD_NEK2_2	187	192	PF00069	0.670
MOD_OFUCOSY	84	91	PF10250	0.554
MOD_PIKK_1	12	18	PF00454	0.567
MOD_PIKK_1	170	176	PF00454	0.529
MOD_Plk_1	127	133	PF00069	0.433
MOD_Plk_1	134	140	PF00069	0.363
MOD_Plk_1	178	184	PF00069	0.633
MOD_Plk_4	127	133	PF00069	0.461
MOD_Plk_4	178	184	PF00069	0.556
MOD_ProDKin_1	166	172	PF00069	0.451
TRG_ENDOCYTIC_2	120	123	PF00928	0.447
TRG_ER_diArg_1	145	147	PF00400	0.580
TRG_ER_diArg_1	201	203	PF00400	0.646

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1Y6	Leptomonas seymouri	52%	92%
A0A0S4JRC5	Bodo saltans	30%	84%
A0A1X0NEC8	Trypanosomatidae	43%	95%
A0A3Q8IER4	Leishmania donovani	43%	100%
A0A3S7X3B7	Leishmania donovani	60%	100%
A4HI90	Leishmania braziliensis	43%	100%
A4HI92	Leishmania braziliensis	76%	100%
A4I5H0	Leishmania infantum	43%	100%
E9AHJ8	Leishmania infantum	59%	100%
E9AHJ9	Leishmania infantum	55%	100%
E9AHK0	Leishmania infantum	57%	99%
E9B0R8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	47%	100%
E9B0S0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	71%	100%
E9B0S1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	66%	100%
Q4Q7G1	Leishmania major	63%	100%
Q4Q7G2	Leishmania major	60%	100%
Q4Q7G3	Leishmania major	68%	100%
Q4Q7G5	Leishmania major	38%	100%