N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glycosyltransferase family 28 protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HI04_LEIBR

TriTrypDb:

LbrM.30.0610 , LBRM2903_300011900

Length:

436

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0016020	membrane	2	12
GO:0031090	organelle membrane	3	12
GO:0031965	nuclear membrane	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HI04

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HI04

Function

Biological processes
Term	Name	Level	Count
GO:0006488	dolichol-linked oligosaccharide biosynthetic process	5	12
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	12
GO:0006629	lipid metabolic process	3	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009987	cellular process	1	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044255	cellular lipid metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:1901135	carbohydrate derivative metabolic process	3	12
GO:1901137	carbohydrate derivative biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004577	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	6	9
GO:0008194	UDP-glycosyltransferase activity	4	9
GO:0008375	acetylglucosaminyltransferase activity	5	9
GO:0016740	transferase activity	2	12
GO:0016757	glycosyltransferase activity	3	12
GO:0016758	hexosyltransferase activity	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	408	412	PF00656	0.470
CLV_NRD_NRD_1	210	212	PF00675	0.328
CLV_NRD_NRD_1	311	313	PF00675	0.200
CLV_NRD_NRD_1	93	95	PF00675	0.310
CLV_PCSK_FUR_1	207	211	PF00082	0.362
CLV_PCSK_KEX2_1	207	209	PF00082	0.293
CLV_PCSK_KEX2_1	210	212	PF00082	0.291
CLV_PCSK_KEX2_1	275	277	PF00082	0.313
CLV_PCSK_KEX2_1	311	313	PF00082	0.200
CLV_PCSK_KEX2_1	93	95	PF00082	0.310
CLV_PCSK_PC1ET2_1	275	277	PF00082	0.327
CLV_PCSK_PC7_1	203	209	PF00082	0.366
CLV_PCSK_SKI1_1	334	338	PF00082	0.325
CLV_PCSK_SKI1_1	416	420	PF00082	0.355
CLV_Separin_Metazoa	50	54	PF03568	0.501
DEG_APCC_DBOX_1	210	218	PF00400	0.599
DEG_APCC_DBOX_1	325	333	PF00400	0.487
DEG_APCC_DBOX_1	394	402	PF00400	0.608
DEG_APCC_DBOX_1	415	423	PF00400	0.506
DEG_MDM2_SWIB_1	194	201	PF02201	0.517
DEG_Nend_Nbox_1	1	3	PF02207	0.340
DEG_SPOP_SBC_1	71	75	PF00917	0.454
DOC_CYCLIN_yCln2_LP_2	185	191	PF00134	0.562
DOC_MAPK_gen_1	207	217	PF00069	0.510
DOC_MAPK_MEF2A_6	33	42	PF00069	0.585
DOC_MAPK_MEF2A_6	416	423	PF00069	0.554
DOC_MAPK_NFAT4_5	416	424	PF00069	0.556
DOC_PP2B_LxvP_1	185	188	PF13499	0.501
DOC_USP7_MATH_1	304	308	PF00917	0.581
DOC_WW_Pin1_4	27	32	PF00397	0.636
LIG_14-3-3_CanoR_1	126	136	PF00244	0.474
LIG_14-3-3_CanoR_1	231	236	PF00244	0.633
LIG_14-3-3_CanoR_1	334	339	PF00244	0.562
LIG_14-3-3_CanoR_1	364	374	PF00244	0.513
LIG_14-3-3_CterR_2	432	436	PF00244	0.751
LIG_BRCT_BRCA1_1	248	252	PF00533	0.466
LIG_FHA_1	110	116	PF00498	0.587
LIG_FHA_1	180	186	PF00498	0.550
LIG_FHA_1	282	288	PF00498	0.512
LIG_FHA_1	335	341	PF00498	0.556
LIG_FHA_1	391	397	PF00498	0.479
LIG_FHA_2	345	351	PF00498	0.476
LIG_GBD_Chelix_1	114	122	PF00786	0.223
LIG_LIR_Gen_1	171	181	PF02991	0.476
LIG_LIR_Gen_1	240	247	PF02991	0.545
LIG_LIR_Nem_3	15	20	PF02991	0.475
LIG_LIR_Nem_3	171	176	PF02991	0.476
LIG_LIR_Nem_3	195	201	PF02991	0.487
LIG_LIR_Nem_3	240	245	PF02991	0.531
LIG_LIR_Nem_3	249	255	PF02991	0.438
LIG_LIR_Nem_3	66	72	PF02991	0.562
LIG_NRBOX	3	9	PF00104	0.399
LIG_PCNA_yPIPBox_3	364	378	PF02747	0.580
LIG_Pex14_2	194	198	PF04695	0.508
LIG_SH2_CRK	162	166	PF00017	0.501
LIG_SH2_CRK	242	246	PF00017	0.559
LIG_SH2_NCK_1	242	246	PF00017	0.562
LIG_SH2_PTP2	23	26	PF00017	0.631
LIG_SH2_STAT5	110	113	PF00017	0.476
LIG_SH2_STAT5	216	219	PF00017	0.559
LIG_SH2_STAT5	23	26	PF00017	0.631
LIG_SH2_STAT5	278	281	PF00017	0.532
LIG_SH2_STAT5	70	73	PF00017	0.476
LIG_SH3_3	140	146	PF00018	0.292
LIG_SH3_3	173	179	PF00018	0.497
LIG_SH3_3	356	362	PF00018	0.504
LIG_Sin3_3	336	343	PF02671	0.476
LIG_SUMO_SIM_par_1	24	30	PF11976	0.525
LIG_SUMO_SIM_par_1	243	250	PF11976	0.476
LIG_SUMO_SIM_par_1	336	341	PF11976	0.547
LIG_SUMO_SIM_par_1	397	402	PF11976	0.623
LIG_TRAF2_1	237	240	PF00917	0.687
LIG_TYR_ITIM	21	26	PF00017	0.631
LIG_WRC_WIRS_1	170	175	PF05994	0.501
MOD_CDC14_SPxK_1	30	33	PF00782	0.670
MOD_CDK_SPxK_1	27	33	PF00069	0.647
MOD_CK1_1	109	115	PF00069	0.551
MOD_CK1_1	177	183	PF00069	0.566
MOD_CK1_1	307	313	PF00069	0.587
MOD_CK1_1	81	87	PF00069	0.581
MOD_CK2_1	344	350	PF00069	0.476
MOD_CK2_1	81	87	PF00069	0.549
MOD_GlcNHglycan	407	410	PF01048	0.403
MOD_GlcNHglycan	426	429	PF01048	0.483
MOD_GlcNHglycan	43	46	PF01048	0.300
MOD_GSK3_1	106	113	PF00069	0.477
MOD_GSK3_1	114	121	PF00069	0.492
MOD_GSK3_1	243	250	PF00069	0.480
MOD_GSK3_1	293	300	PF00069	0.548
MOD_GSK3_1	334	341	PF00069	0.549
MOD_GSK3_1	41	48	PF00069	0.498
MOD_GSK3_1	72	79	PF00069	0.488
MOD_NEK2_1	118	123	PF00069	0.386
MOD_NEK2_1	12	17	PF00069	0.411
MOD_NEK2_1	338	343	PF00069	0.479
MOD_NEK2_1	399	404	PF00069	0.602
MOD_NEK2_1	421	426	PF00069	0.642
MOD_PIKK_1	81	87	PF00454	0.581
MOD_PKA_1	210	216	PF00069	0.562
MOD_PKA_2	210	216	PF00069	0.551
MOD_PKA_2	222	228	PF00069	0.631
MOD_PKB_1	208	216	PF00069	0.581
MOD_Plk_1	290	296	PF00069	0.565
MOD_Plk_1	399	405	PF00069	0.582
MOD_Plk_4	106	112	PF00069	0.487
MOD_Plk_4	12	18	PF00069	0.396
MOD_Plk_4	210	216	PF00069	0.521
MOD_Plk_4	251	257	PF00069	0.537
MOD_Plk_4	290	296	PF00069	0.558
MOD_Plk_4	334	340	PF00069	0.508
MOD_Plk_4	344	350	PF00069	0.438
MOD_Plk_4	58	64	PF00069	0.538
MOD_ProDKin_1	27	33	PF00069	0.647
TRG_DiLeu_BaEn_1	240	245	PF01217	0.558
TRG_DiLeu_BaEn_1	417	422	PF01217	0.623
TRG_DiLeu_BaLyEn_6	181	186	PF01217	0.453
TRG_DiLeu_LyEn_5	394	399	PF01217	0.639
TRG_ENDOCYTIC_2	23	26	PF00928	0.631
TRG_ENDOCYTIC_2	242	245	PF00928	0.590
TRG_ER_diArg_1	101	104	PF00400	0.476
TRG_ER_diArg_1	207	210	PF00400	0.521
TRG_ER_diArg_1	311	313	PF00400	0.457
TRG_Pf-PMV_PEXEL_1	233	238	PF00026	0.493
TRG_Pf-PMV_PEXEL_1	364	369	PF00026	0.282
TRG_Pf-PMV_PEXEL_1	53	57	PF00026	0.307
TRG_Pf-PMV_PEXEL_1	80	85	PF00026	0.200

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I7X1	Leptomonas seymouri	62%	100%
A0A0S4IKP3	Bodo saltans	42%	100%
A0A1X0P2G9	Trypanosomatidae	47%	100%
A0A3Q8IBT8	Leishmania donovani	84%	100%
A0A422NIG8	Trypanosoma rangeli	49%	100%
A4I581	Leishmania infantum	84%	100%
C9ZQL0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	96%
E9B0H8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4Q7Q3	Leishmania major	84%	100%
V5BC77	Trypanosoma cruzi	49%	100%