Cysteine protease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Cysteine protease

Gene product:

AUT2/APG4/ATG4 cysteine peptidase, putative

Species:

Leishmania braziliensis

UniProt:

A4HHX1_LEIBR

TriTrypDb:

LbrM.30.0280 , LBRM2903_300008500

Length:

398

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A4HHX1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HHX1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006810	transport	3	12
GO:0006914	autophagy	3	11
GO:0008104	protein localization	4	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	11
GO:0009987	cellular process	1	12
GO:0015031	protein transport	4	12
GO:0019538	protein metabolic process	3	12
GO:0033036	macromolecule localization	2	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	11
GO:0045184	establishment of protein localization	3	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	12
GO:0061919	process utilizing autophagic mechanism	2	11
GO:0070727	cellular macromolecule localization	3	12
GO:0071702	organic substance transport	4	12
GO:0071704	organic substance metabolic process	2	12
GO:0071705	nitrogen compound transport	4	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000045	autophagosome assembly	6	1
GO:0006996	organelle organization	4	1
GO:0007033	vacuole organization	5	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1905037	autophagosome organization	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008233	peptidase activity	3	12
GO:0008234	cysteine-type peptidase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	141	145	PF00656	0.378
CLV_C14_Caspase3-7	374	378	PF00656	0.750
CLV_C14_Caspase3-7	49	53	PF00656	0.559
CLV_NRD_NRD_1	357	359	PF00675	0.727
CLV_NRD_NRD_1	366	368	PF00675	0.753
CLV_NRD_NRD_1	94	96	PF00675	0.341
CLV_PCSK_FUR_1	364	368	PF00082	0.522
CLV_PCSK_KEX2_1	139	141	PF00082	0.264
CLV_PCSK_KEX2_1	357	359	PF00082	0.706
CLV_PCSK_KEX2_1	366	368	PF00082	0.687
CLV_PCSK_KEX2_1	94	96	PF00082	0.262
CLV_PCSK_PC1ET2_1	139	141	PF00082	0.344
CLV_PCSK_PC7_1	90	96	PF00082	0.262
CLV_PCSK_SKI1_1	122	126	PF00082	0.291
CLV_PCSK_SKI1_1	95	99	PF00082	0.178
DEG_Nend_Nbox_1	1	3	PF02207	0.625
DEG_SCF_FBW7_1	256	263	PF00400	0.378
DEG_SPOP_SBC_1	157	161	PF00917	0.531
DOC_AGCK_PIF_1	45	50	PF00069	0.495
DOC_MAPK_gen_1	94	100	PF00069	0.559
DOC_MAPK_MEF2A_6	175	184	PF00069	0.540
DOC_MAPK_MEF2A_6	272	281	PF00069	0.530
DOC_PP1_RVXF_1	264	271	PF00149	0.479
DOC_PP2B_LxvP_1	360	363	PF13499	0.719
DOC_PP4_FxxP_1	327	330	PF00568	0.723
DOC_USP7_MATH_1	220	224	PF00917	0.463
DOC_USP7_MATH_1	254	258	PF00917	0.493
DOC_USP7_MATH_1	260	264	PF00917	0.452
DOC_USP7_MATH_1	30	34	PF00917	0.540
DOC_WW_Pin1_4	127	132	PF00397	0.472
DOC_WW_Pin1_4	256	261	PF00397	0.517
DOC_WW_Pin1_4	326	331	PF00397	0.707
LIG_14-3-3_CanoR_1	154	158	PF00244	0.420
LIG_14-3-3_CanoR_1	188	195	PF00244	0.490
LIG_14-3-3_CanoR_1	242	248	PF00244	0.544
LIG_14-3-3_CanoR_1	357	361	PF00244	0.730
LIG_14-3-3_CanoR_1	75	81	PF00244	0.465
LIG_APCC_ABBA_1	96	101	PF00400	0.559
LIG_APCC_ABBAyCdc20_2	95	101	PF00400	0.559
LIG_BRCT_BRCA1_1	222	226	PF00533	0.540
LIG_BRCT_BRCA1_1	296	300	PF00533	0.547
LIG_BRCT_BRCA1_1	9	13	PF00533	0.579
LIG_Clathr_ClatBox_1	234	238	PF01394	0.465
LIG_deltaCOP1_diTrp_1	7	13	PF00928	0.583
LIG_EH1_1	108	116	PF00400	0.515
LIG_FHA_1	13	19	PF00498	0.524
LIG_FHA_1	146	152	PF00498	0.540
LIG_FHA_1	242	248	PF00498	0.503
LIG_FHA_1	253	259	PF00498	0.460
LIG_FHA_1	349	355	PF00498	0.730
LIG_FHA_1	357	363	PF00498	0.711
LIG_FHA_1	42	48	PF00498	0.465
LIG_FHA_1	59	65	PF00498	0.436
LIG_FHA_2	139	145	PF00498	0.475
LIG_FHA_2	282	288	PF00498	0.516
LIG_FHA_2	372	378	PF00498	0.533
LIG_FHA_2	47	53	PF00498	0.540
LIG_LIR_Apic_2	127	131	PF02991	0.482
LIG_LIR_Apic_2	326	330	PF02991	0.725
LIG_LIR_Gen_1	198	207	PF02991	0.516
LIG_LIR_Gen_1	275	283	PF02991	0.483
LIG_LIR_Gen_1	287	296	PF02991	0.451
LIG_LIR_Gen_1	297	304	PF02991	0.409
LIG_LIR_Gen_1	391	398	PF02991	0.721
LIG_LIR_Gen_1	43	50	PF02991	0.447
LIG_LIR_Gen_1	52	60	PF02991	0.569
LIG_LIR_Gen_1	7	17	PF02991	0.564
LIG_LIR_Nem_3	198	203	PF02991	0.516
LIG_LIR_Nem_3	223	227	PF02991	0.454
LIG_LIR_Nem_3	275	281	PF02991	0.463
LIG_LIR_Nem_3	287	292	PF02991	0.507
LIG_LIR_Nem_3	297	303	PF02991	0.407
LIG_LIR_Nem_3	391	397	PF02991	0.723
LIG_LIR_Nem_3	43	48	PF02991	0.358
LIG_LIR_Nem_3	52	57	PF02991	0.441
LIG_LIR_Nem_3	67	73	PF02991	0.415
LIG_LIR_Nem_3	7	12	PF02991	0.559
LIG_MLH1_MIPbox_1	222	226	PF16413	0.540
LIG_MLH1_MIPbox_1	9	13	PF16413	0.579
LIG_PCNA_PIPBox_1	78	87	PF02747	0.540
LIG_PCNA_yPIPBox_3	75	85	PF02747	0.515
LIG_Pex14_1	9	13	PF04695	0.552
LIG_SH2_CRK	88	92	PF00017	0.364
LIG_SH2_SRC	236	239	PF00017	0.482
LIG_SH2_STAP1	166	170	PF00017	0.540
LIG_SH2_STAT5	200	203	PF00017	0.528
LIG_SH2_STAT5	225	228	PF00017	0.454
LIG_SH2_STAT5	236	239	PF00017	0.444
LIG_SH2_STAT5	4	7	PF00017	0.582
LIG_SUMO_SIM_anti_2	153	161	PF11976	0.497
LIG_SUMO_SIM_anti_2	179	186	PF11976	0.497
LIG_SUMO_SIM_par_1	179	186	PF11976	0.499
LIG_SUMO_SIM_par_1	253	259	PF11976	0.488
LIG_TRAF2_1	33	36	PF00917	0.529
LIG_TRFH_1	235	239	PF08558	0.559
MOD_CDC14_SPxK_1	329	332	PF00782	0.500
MOD_CDK_SPxK_1	326	332	PF00069	0.501
MOD_CK1_1	103	109	PF00069	0.524
MOD_CK2_1	282	288	PF00069	0.480
MOD_CK2_1	30	36	PF00069	0.466
MOD_Cter_Amidation	364	367	PF01082	0.522
MOD_GlcNHglycan	101	105	PF01048	0.308
MOD_GlcNHglycan	189	192	PF01048	0.340
MOD_GlcNHglycan	252	255	PF01048	0.323
MOD_GlcNHglycan	26	29	PF01048	0.527
MOD_GlcNHglycan	9	12	PF01048	0.510
MOD_GSK3_1	103	110	PF00069	0.509
MOD_GSK3_1	153	160	PF00069	0.416
MOD_GSK3_1	183	190	PF00069	0.493
MOD_GSK3_1	246	253	PF00069	0.506
MOD_GSK3_1	254	261	PF00069	0.462
MOD_GSK3_1	268	275	PF00069	0.463
MOD_N-GLC_1	229	234	PF02516	0.312
MOD_N-GLC_1	323	328	PF02516	0.501
MOD_N-GLC_2	162	164	PF02516	0.340
MOD_N-GLC_2	335	337	PF02516	0.501
MOD_NEK2_1	114	119	PF00069	0.547
MOD_NEK2_1	281	286	PF00069	0.518
MOD_NEK2_1	348	353	PF00069	0.688
MOD_NEK2_1	41	46	PF00069	0.401
MOD_NEK2_1	73	78	PF00069	0.458
MOD_PIKK_1	129	135	PF00454	0.465
MOD_PIKK_1	193	199	PF00454	0.479
MOD_PIKK_1	229	235	PF00454	0.485
MOD_PIKK_1	30	36	PF00454	0.500
MOD_PIKK_1	302	308	PF00454	0.602
MOD_PIKK_1	74	80	PF00454	0.465
MOD_PKA_2	153	159	PF00069	0.450
MOD_PKA_2	187	193	PF00069	0.522
MOD_PKA_2	241	247	PF00069	0.544
MOD_PKA_2	356	362	PF00069	0.727
MOD_PKA_2	74	80	PF00069	0.465
MOD_Plk_1	229	235	PF00069	0.512
MOD_Plk_1	273	279	PF00069	0.454
MOD_Plk_2-3	390	396	PF00069	0.723
MOD_Plk_4	153	159	PF00069	0.408
MOD_Plk_4	196	202	PF00069	0.549
MOD_Plk_4	273	279	PF00069	0.478
MOD_Plk_4	323	329	PF00069	0.694
MOD_Plk_4	41	47	PF00069	0.419
MOD_ProDKin_1	127	133	PF00069	0.472
MOD_ProDKin_1	256	262	PF00069	0.517
MOD_ProDKin_1	326	332	PF00069	0.708
MOD_SUMO_for_1	124	127	PF00179	0.540
MOD_SUMO_for_1	146	149	PF00179	0.559
TRG_DiLeu_BaEn_1	350	355	PF01217	0.627
TRG_DiLeu_BaLyEn_6	59	64	PF01217	0.540
TRG_ENDOCYTIC_2	200	203	PF00928	0.540
TRG_ENDOCYTIC_2	224	227	PF00928	0.445
TRG_ER_diArg_1	363	366	PF00400	0.743
TRG_ER_diArg_1	94	96	PF00400	0.462
TRG_Pf-PMV_PEXEL_1	307	311	PF00026	0.426

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A098DRK7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	26%	85%
A0A0G2QC33	Rattus norvegicus	24%	100%
A0A0N0P8C0	Leptomonas seymouri	63%	99%
A0A0S4JSC7	Bodo saltans	36%	100%
A0A1X0P2H1	Trypanosomatidae	37%	100%
A0A3R7NRV7	Trypanosoma rangeli	42%	100%
A0A3S7X2Y6	Leishmania donovani	84%	100%
A1CJ08	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	27%	100%
A2QY50	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	26%	99%
A4I521	Leishmania infantum	85%	100%
A4I8K7	Leishmania infantum	25%	100%
A5DEF7	Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324)	27%	99%
A7KAI3	Pichia angusta	31%	78%
C9ZQI0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E2RDP2	Canis lupus familiaris	26%	84%
E9B0F1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
K8ESC5	Caenorhabditis elegans	25%	83%
Q1E5M9	Coccidioides immitis (strain RS)	28%	92%
Q2HH40	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	27%	89%
Q2U5B0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	28%	98%
Q4Q7T2	Leishmania major	84%	99%
Q4U3V5	Cryphonectria parasitica	27%	87%
Q523C3	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	28%	81%
Q5B7L0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	25%	99%
Q5XH30	Xenopus laevis	23%	88%
Q684M2	Sus scrofa	26%	85%
Q68FJ9	Xenopus laevis	23%	85%
Q6CH28	Yarrowia lipolytica (strain CLIB 122 / E 150)	25%	73%
Q6FP20	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	22%	82%
Q6PZ02	Gallus gallus	25%	100%
Q75E61	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	29%	76%
Q86TL0	Homo sapiens	26%	84%
Q86ZL5	Podospora anserina	25%	80%
Q8BGE6	Mus musculus	25%	100%
Q8BGV9	Mus musculus	25%	84%
Q9P373	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9U1N6	Caenorhabditis elegans	25%	76%
Q9Y4P1	Homo sapiens	25%	100%
V5BGZ3	Trypanosoma cruzi	39%	100%
W0TGM7	Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275)	24%	88%