Polynucleotide adenylyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly(A) polymerase, putative

Species:

Leishmania braziliensis

UniProt:

A4HHQ8_LEIBR

TriTrypDb:

LbrM.29.2570 , LBRM2903_290032900

Length:

417

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	9
GO:0005737	cytoplasm	2	8
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	9
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	9
GO:0110165	cellular anatomical entity	1	9
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

A4HHQ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HHQ8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006396	RNA processing	6	9
GO:0006397	mRNA processing	7	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0016071	mRNA metabolic process	6	9
GO:0031123	RNA 3'-end processing	7	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043631	RNA polyadenylation	6	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071076	RNA 3' uridylation	8	8
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:0006378	mRNA polyadenylation	7	1
GO:0031124	mRNA 3'-end processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003676	nucleic acid binding	3	9
GO:0003723	RNA binding	4	9
GO:0003824	catalytic activity	1	9
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0016740	transferase activity	2	9
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	9
GO:0016779	nucleotidyltransferase activity	4	9
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0050265	RNA uridylyltransferase activity	4	8
GO:0070566	adenylyltransferase activity	5	9
GO:0070569	uridylyltransferase activity	5	8
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	4	6	PF00675	0.329
CLV_NRD_NRD_1	99	101	PF00675	0.326
CLV_PCSK_KEX2_1	4	6	PF00082	0.329
CLV_PCSK_KEX2_1	98	100	PF00082	0.326
CLV_PCSK_SKI1_1	113	117	PF00082	0.344
CLV_PCSK_SKI1_1	158	162	PF00082	0.311
CLV_PCSK_SKI1_1	234	238	PF00082	0.476
CLV_PCSK_SKI1_1	322	326	PF00082	0.557
CLV_PCSK_SKI1_1	336	340	PF00082	0.315
CLV_PCSK_SKI1_1	5	9	PF00082	0.304
CLV_PCSK_SKI1_1	92	96	PF00082	0.375
CLV_Separin_Metazoa	235	239	PF03568	0.417
CLV_Separin_Metazoa	70	74	PF03568	0.476
DOC_AGCK_PIF_2	358	363	PF00069	0.557
DOC_CDC14_PxL_1	19	27	PF14671	0.330
DOC_CYCLIN_RxL_1	231	240	PF00134	0.476
DOC_CYCLIN_RxL_1	96	107	PF00134	0.359
DOC_CYCLIN_yCln2_LP_2	253	259	PF00134	0.455
DOC_MAPK_gen_1	170	179	PF00069	0.344
DOC_MAPK_gen_1	98	105	PF00069	0.387
DOC_MAPK_MEF2A_6	226	233	PF00069	0.476
DOC_MAPK_MEF2A_6	264	271	PF00069	0.417
DOC_MAPK_MEF2A_6	49	57	PF00069	0.400
DOC_MAPK_MEF2A_6	98	105	PF00069	0.383
DOC_MAPK_NFAT4_5	226	234	PF00069	0.476
DOC_MAPK_NFAT4_5	98	106	PF00069	0.476
DOC_PP1_RVXF_1	384	390	PF00149	0.462
DOC_PP2B_LxvP_1	101	104	PF13499	0.395
DOC_PP4_FxxP_1	312	315	PF00568	0.508
DOC_PP4_MxPP_1	194	197	PF00568	0.476
DOC_SPAK_OSR1_1	264	268	PF12202	0.476
DOC_USP7_MATH_1	104	108	PF00917	0.456
DOC_USP7_MATH_1	66	70	PF00917	0.367
DOC_USP7_MATH_1	7	11	PF00917	0.541
DOC_USP7_UBL2_3	45	49	PF12436	0.304
DOC_WW_Pin1_4	317	322	PF00397	0.365
DOC_WW_Pin1_4	397	402	PF00397	0.538
LIG_14-3-3_CanoR_1	158	164	PF00244	0.476
LIG_14-3-3_CanoR_1	24	30	PF00244	0.344
LIG_14-3-3_CanoR_1	326	332	PF00244	0.552
LIG_14-3-3_CanoR_1	386	390	PF00244	0.489
LIG_14-3-3_CanoR_1	85	89	PF00244	0.354
LIG_Actin_WH2_2	156	172	PF00022	0.417
LIG_BRCT_BRCA1_1	334	338	PF00533	0.395
LIG_deltaCOP1_diTrp_1	199	208	PF00928	0.476
LIG_EH_1	372	376	PF12763	0.633
LIG_FHA_1	66	72	PF00498	0.387
LIG_FHA_2	170	176	PF00498	0.299
LIG_FHA_2	62	68	PF00498	0.433
LIG_FHA_2	91	97	PF00498	0.375
LIG_LIR_Gen_1	106	115	PF02991	0.344
LIG_LIR_Gen_1	16	25	PF02991	0.477
LIG_LIR_Gen_1	249	257	PF02991	0.400
LIG_LIR_Gen_1	355	365	PF02991	0.315
LIG_LIR_Gen_1	54	65	PF02991	0.477
LIG_LIR_Gen_1	74	83	PF02991	0.126
LIG_LIR_Nem_3	106	111	PF02991	0.344
LIG_LIR_Nem_3	16	22	PF02991	0.469
LIG_LIR_Nem_3	206	211	PF02991	0.375
LIG_LIR_Nem_3	249	253	PF02991	0.415
LIG_LIR_Nem_3	355	361	PF02991	0.545
LIG_LIR_Nem_3	388	393	PF02991	0.526
LIG_LIR_Nem_3	47	51	PF02991	0.511
LIG_LIR_Nem_3	54	60	PF02991	0.403
LIG_LIR_Nem_3	74	79	PF02991	0.126
LIG_LRP6_Inhibitor_1	86	92	PF00058	0.476
LIG_MYND_1	209	213	PF01753	0.375
LIG_PDZ_Class_1	412	417	PF00595	0.615
LIG_REV1ctd_RIR_1	16	22	PF16727	0.448
LIG_REV1ctd_RIR_1	230	238	PF16727	0.242
LIG_REV1ctd_RIR_1	337	347	PF16727	0.482
LIG_SH2_CRK	108	112	PF00017	0.456
LIG_SH2_CRK	125	129	PF00017	0.207
LIG_SH2_CRK	393	397	PF00017	0.574
LIG_SH2_CRK	48	52	PF00017	0.563
LIG_SH2_SRC	291	294	PF00017	0.476
LIG_SH2_STAP1	108	112	PF00017	0.417
LIG_SH2_STAP1	250	254	PF00017	0.510
LIG_SH2_STAP1	406	410	PF00017	0.491
LIG_SH2_STAT5	129	132	PF00017	0.344
LIG_SH2_STAT5	164	167	PF00017	0.344
LIG_SH2_STAT5	291	294	PF00017	0.375
LIG_SH3_3	186	192	PF00018	0.347
LIG_SH3_3	207	213	PF00018	0.320
LIG_SH3_3	77	83	PF00018	0.476
LIG_SUMO_SIM_anti_2	175	181	PF11976	0.375
LIG_SUMO_SIM_anti_2	32	38	PF11976	0.563
LIG_SUMO_SIM_anti_2	74	81	PF11976	0.449
LIG_SUMO_SIM_par_1	32	38	PF11976	0.573
LIG_TRAF2_1	181	184	PF00917	0.344
LIG_TYR_ITIM	248	253	PF00017	0.307
LIG_WRPW_2	254	257	PF00400	0.393
MOD_CDK_SPK_2	317	322	PF00069	0.365
MOD_CK1_1	12	18	PF00069	0.494
MOD_CK1_1	327	333	PF00069	0.335
MOD_CK1_1	388	394	PF00069	0.443
MOD_CK1_1	78	84	PF00069	0.422
MOD_CK2_1	246	252	PF00069	0.401
MOD_CK2_1	317	323	PF00069	0.368
MOD_CK2_1	349	355	PF00069	0.529
MOD_CK2_1	356	362	PF00069	0.427
MOD_CK2_1	90	96	PF00069	0.375
MOD_GlcNHglycan	152	155	PF01048	0.364
MOD_GlcNHglycan	226	229	PF01048	0.375
MOD_GlcNHglycan	326	329	PF01048	0.490
MOD_GlcNHglycan	37	40	PF01048	0.474
MOD_GlcNHglycan	406	409	PF01048	0.494
MOD_GlcNHglycan	414	417	PF01048	0.625
MOD_GlcNHglycan	9	12	PF01048	0.517
MOD_GSK3_1	242	249	PF00069	0.289
MOD_GSK3_1	363	370	PF00069	0.511
MOD_GSK3_1	61	68	PF00069	0.465
MOD_GSK3_1	9	16	PF00069	0.479
MOD_N-GLC_1	13	18	PF02516	0.545
MOD_NEK2_1	159	164	PF00069	0.367
MOD_NEK2_1	169	174	PF00069	0.310
MOD_NEK2_1	242	247	PF00069	0.384
MOD_NEK2_1	361	366	PF00069	0.317
MOD_NEK2_2	385	390	PF00069	0.461
MOD_PKA_2	169	175	PF00069	0.375
MOD_PKA_2	23	29	PF00069	0.346
MOD_PKA_2	385	391	PF00069	0.518
MOD_PKA_2	84	90	PF00069	0.353
MOD_Plk_1	13	19	PF00069	0.540
MOD_Plk_1	281	287	PF00069	0.359
MOD_Plk_1	361	367	PF00069	0.475
MOD_Plk_4	13	19	PF00069	0.472
MOD_Plk_4	175	181	PF00069	0.476
MOD_Plk_4	385	391	PF00069	0.518
MOD_Plk_4	75	81	PF00069	0.357
MOD_ProDKin_1	317	323	PF00069	0.368
MOD_ProDKin_1	397	403	PF00069	0.538
MOD_SUMO_for_1	181	184	PF00179	0.242
TRG_DiLeu_BaEn_2	261	267	PF01217	0.375
TRG_DiLeu_BaEn_3	183	189	PF01217	0.242
TRG_DiLeu_BaLyEn_6	39	44	PF01217	0.543
TRG_ENDOCYTIC_2	108	111	PF00928	0.365
TRG_ENDOCYTIC_2	125	128	PF00928	0.178
TRG_ENDOCYTIC_2	250	253	PF00928	0.445
TRG_ENDOCYTIC_2	257	260	PF00928	0.332
TRG_ENDOCYTIC_2	393	396	PF00928	0.574
TRG_ENDOCYTIC_2	48	51	PF00928	0.566
TRG_ER_diArg_1	3	5	PF00400	0.332
TRG_ER_diArg_1	97	100	PF00400	0.331
TRG_Pf-PMV_PEXEL_1	92	96	PF00026	0.476

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1ILG5	Leptomonas seymouri	53%	100%
A0A0S4IKH6	Bodo saltans	37%	76%
A4I4T6	Leishmania infantum	90%	83%
C9ZKY5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	86%
E9AEC2	Leishmania major	89%	100%
E9ALG4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	83%
O42617	Candida albicans (strain SC5314 / ATCC MYA-2876)	28%	75%
P29468	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	73%
Q10295	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	74%
Q51D88	Entamoeba histolytica	24%	80%
Q56XM9	Arabidopsis thaliana	25%	82%
Q9UW26	Candida albicans (strain SC5314 / ATCC MYA-2876)	32%	75%
V5DTV7	Trypanosoma cruzi	43%	77%