Fumarate hydratase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Fumarate hydratase

Gene product:

Fumarate hydratase class I, cytosolic

Species:

Leishmania braziliensis

UniProt:

A4HHJ6_LEIBR

TriTrypDb:

LbrM.29.1930 , LBRM2903_290025600

Length:

568

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005777	peroxisome	6	1
GO:0005829	cytosol	2	1
GO:0020015	glycosome	7	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HHJ6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HHJ6

PDB structure(s): 5l2r_A , 5l2r_B , 6msn_A , 6msn_B , 6unz_A , 6unz_B , 6unz_C , 6unz_D , 6unz_E , 6unz_F , 6unz_G , 6unz_H , 6uo0_A , 6uo0_B , 6uoi_A , 6uoi_B , 6uoj_A , 6uoj_B , 6up9_A , 6up9_B , 6upm_A , 6upm_B , 6upo_A , 6upo_B , 6uq8_A , 6uq8_B , 6uq9_A , 6uq9_B , 6uqb_A , 6uqb_B , 6uql_A , 6uql_B , 6uqm_A , 6uqm_B , 6uqn_A , 6uqn_B

Function

Biological processes
Term	Name	Level	Count
GO:0006091	generation of precursor metabolites and energy	3	10
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0044237	cellular metabolic process	2	10
GO:0006082	organic acid metabolic process	3	1
GO:0006106	fumarate metabolic process	7	1
GO:0006108	malate metabolic process	7	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0043436	oxoacid metabolic process	4	1
GO:0043648	dicarboxylic acid metabolic process	6	1
GO:0044281	small molecule metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004333	fumarate hydratase activity	5	10
GO:0005488	binding	1	10
GO:0016829	lyase activity	2	10
GO:0016835	carbon-oxygen lyase activity	3	10
GO:0016836	hydro-lyase activity	4	10
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051539	4 iron, 4 sulfur cluster binding	4	10
GO:0051540	metal cluster binding	2	10
GO:0005515	protein binding	2	1
GO:0042802	identical protein binding	3	1
GO:0042803	protein homodimerization activity	4	1
GO:0046983	protein dimerization activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	106	110	PF00656	0.239
CLV_C14_Caspase3-7	430	434	PF00656	0.345
CLV_NRD_NRD_1	399	401	PF00675	0.190
CLV_NRD_NRD_1	90	92	PF00675	0.232
CLV_NRD_NRD_1	99	101	PF00675	0.219
CLV_PCSK_KEX2_1	144	146	PF00082	0.227
CLV_PCSK_KEX2_1	392	394	PF00082	0.370
CLV_PCSK_KEX2_1	399	401	PF00082	0.420
CLV_PCSK_KEX2_1	99	101	PF00082	0.231
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.227
CLV_PCSK_PC1ET2_1	392	394	PF00082	0.370
CLV_PCSK_SKI1_1	228	232	PF00082	0.249
CLV_PCSK_SKI1_1	271	275	PF00082	0.263
CLV_PCSK_SKI1_1	350	354	PF00082	0.227
CLV_PCSK_SKI1_1	410	414	PF00082	0.261
CLV_PCSK_SKI1_1	429	433	PF00082	0.500
CLV_PCSK_SKI1_1	496	500	PF00082	0.227
CLV_PCSK_SKI1_1	526	530	PF00082	0.240
CLV_PCSK_SKI1_1	65	69	PF00082	0.286
DEG_APCC_DBOX_1	349	357	PF00400	0.227
DOC_MAPK_MEF2A_6	529	538	PF00069	0.185
DOC_PP1_RVXF_1	294	301	PF00149	0.184
DOC_PP1_RVXF_1	527	534	PF00149	0.185
DOC_PP2B_LxvP_1	199	202	PF13499	0.287
DOC_USP7_MATH_1	105	109	PF00917	0.239
DOC_USP7_MATH_1	308	312	PF00917	0.333
DOC_USP7_MATH_1	460	464	PF00917	0.286
DOC_USP7_MATH_1	521	525	PF00917	0.240
DOC_USP7_UBL2_3	157	161	PF12436	0.257
DOC_USP7_UBL2_3	522	526	PF12436	0.345
DOC_WW_Pin1_4	442	447	PF00397	0.257
DOC_WW_Pin1_4	452	457	PF00397	0.231
LIG_14-3-3_CanoR_1	173	183	PF00244	0.227
LIG_14-3-3_CanoR_1	30	37	PF00244	0.281
LIG_14-3-3_CanoR_1	333	339	PF00244	0.227
LIG_14-3-3_CanoR_1	399	407	PF00244	0.217
LIG_14-3-3_CanoR_1	81	90	PF00244	0.281
LIG_APCC_ABBA_1	180	185	PF00400	0.227
LIG_APCC_ABBAyCdc20_2	220	226	PF00400	0.345
LIG_APCC_ABBAyCdc20_2	358	364	PF00400	0.287
LIG_APCC_Cbox_2	111	117	PF00515	0.227
LIG_BIR_II_1	1	5	PF00653	0.458
LIG_BRCT_BRCA1_1	31	35	PF00533	0.393
LIG_BRCT_BRCA1_1	460	464	PF00533	0.227
LIG_deltaCOP1_diTrp_1	539	549	PF00928	0.227
LIG_DLG_GKlike_1	471	478	PF00625	0.345
LIG_eIF4E_1	113	119	PF01652	0.345
LIG_FHA_1	136	142	PF00498	0.228
LIG_FHA_1	175	181	PF00498	0.227
LIG_FHA_1	225	231	PF00498	0.227
LIG_FHA_1	270	276	PF00498	0.227
LIG_FHA_1	377	383	PF00498	0.359
LIG_FHA_1	414	420	PF00498	0.345
LIG_FHA_1	442	448	PF00498	0.240
LIG_FHA_2	149	155	PF00498	0.238
LIG_FHA_2	262	268	PF00498	0.227
LIG_FHA_2	284	290	PF00498	0.345
LIG_FHA_2	46	52	PF00498	0.354
LIG_LIR_Gen_1	111	119	PF02991	0.215
LIG_LIR_Gen_1	204	212	PF02991	0.231
LIG_LIR_Gen_1	28	37	PF02991	0.576
LIG_LIR_Gen_1	297	308	PF02991	0.227
LIG_LIR_Gen_1	44	54	PF02991	0.337
LIG_LIR_Gen_1	473	479	PF02991	0.267
LIG_LIR_Gen_1	561	568	PF02991	0.184
LIG_LIR_Nem_3	204	209	PF02991	0.231
LIG_LIR_Nem_3	28	34	PF02991	0.348
LIG_LIR_Nem_3	297	303	PF02991	0.227
LIG_LIR_Nem_3	44	50	PF02991	0.320
LIG_LIR_Nem_3	473	478	PF02991	0.267
LIG_LIR_Nem_3	548	552	PF02991	0.292
LIG_Pex14_2	31	35	PF04695	0.439
LIG_PTB_Apo_2	168	175	PF02174	0.345
LIG_PTB_Phospho_1	168	174	PF10480	0.345
LIG_REV1ctd_RIR_1	87	96	PF16727	0.239
LIG_RPA_C_Fungi	416	428	PF08784	0.239
LIG_SH2_CRK	169	173	PF00017	0.345
LIG_SH2_CRK	281	285	PF00017	0.227
LIG_SH2_CRK	449	453	PF00017	0.227
LIG_SH2_GRB2like	169	172	PF00017	0.261
LIG_SH2_PTP2	475	478	PF00017	0.239
LIG_SH2_STAP1	174	178	PF00017	0.231
LIG_SH2_STAP1	206	210	PF00017	0.345
LIG_SH2_STAT3	222	225	PF00017	0.345
LIG_SH2_STAT3	362	365	PF00017	0.240
LIG_SH2_STAT5	113	116	PF00017	0.345
LIG_SH2_STAT5	158	161	PF00017	0.234
LIG_SH2_STAT5	222	225	PF00017	0.345
LIG_SH2_STAT5	321	324	PF00017	0.227
LIG_SH2_STAT5	362	365	PF00017	0.344
LIG_SH2_STAT5	373	376	PF00017	0.313
LIG_SH2_STAT5	475	478	PF00017	0.227
LIG_SH2_STAT5	509	512	PF00017	0.227
LIG_SH2_STAT5	56	59	PF00017	0.298
LIG_SH3_3	34	40	PF00018	0.325
LIG_SH3_3	544	550	PF00018	0.192
LIG_SH3_5	437	441	PF00018	0.242
LIG_SUMO_SIM_anti_2	73	79	PF11976	0.296
LIG_UBA3_1	352	358	PF00899	0.301
MOD_CK1_1	283	289	PF00069	0.227
MOD_CK1_1	383	389	PF00069	0.268
MOD_CK2_1	148	154	PF00069	0.227
MOD_CK2_1	201	207	PF00069	0.240
MOD_CK2_1	261	267	PF00069	0.227
MOD_CK2_1	45	51	PF00069	0.357
MOD_CK2_1	521	527	PF00069	0.215
MOD_Cter_Amidation	142	145	PF01082	0.227
MOD_GlcNHglycan	203	206	PF01048	0.250
MOD_GlcNHglycan	216	219	PF01048	0.218
MOD_GlcNHglycan	264	267	PF01048	0.235
MOD_GlcNHglycan	31	34	PF01048	0.379
MOD_GlcNHglycan	310	313	PF01048	0.342
MOD_GlcNHglycan	346	349	PF01048	0.227
MOD_GlcNHglycan	433	436	PF01048	0.234
MOD_GlcNHglycan	460	463	PF01048	0.242
MOD_GSK3_1	129	136	PF00069	0.287
MOD_GSK3_1	262	269	PF00069	0.254
MOD_GSK3_1	280	287	PF00069	0.338
MOD_GSK3_1	376	383	PF00069	0.197
MOD_GSK3_1	460	467	PF00069	0.232
MOD_GSK3_1	478	485	PF00069	0.227
MOD_GSK3_1	492	499	PF00069	0.186
MOD_N-GLC_1	380	385	PF02516	0.228
MOD_N-GLC_1	413	418	PF02516	0.345
MOD_N-GLC_1	492	497	PF02516	0.227
MOD_N-GLC_2	121	123	PF02516	0.227
MOD_NEK2_1	148	153	PF00069	0.239
MOD_NEK2_1	172	177	PF00069	0.235
MOD_NEK2_1	29	34	PF00069	0.511
MOD_NEK2_1	342	347	PF00069	0.227
MOD_NEK2_1	382	387	PF00069	0.470
MOD_NEK2_1	413	418	PF00069	0.231
MOD_NEK2_1	478	483	PF00069	0.240
MOD_NEK2_2	266	271	PF00069	0.227
MOD_NEK2_2	521	526	PF00069	0.240
MOD_PIKK_1	172	178	PF00454	0.227
MOD_PIKK_1	400	406	PF00454	0.239
MOD_PIKK_1	42	48	PF00454	0.308
MOD_PKA_2	172	178	PF00069	0.227
MOD_PKA_2	29	35	PF00069	0.289
MOD_PKA_2	420	426	PF00069	0.226
MOD_Plk_1	266	272	PF00069	0.227
MOD_Plk_4	114	120	PF00069	0.227
MOD_Plk_4	413	419	PF00069	0.239
MOD_Plk_4	471	477	PF00069	0.227
MOD_Plk_4	73	79	PF00069	0.276
MOD_ProDKin_1	442	448	PF00069	0.257
MOD_ProDKin_1	452	458	PF00069	0.231
MOD_SUMO_rev_2	154	159	PF00179	0.239
MOD_SUMO_rev_2	235	244	PF00179	0.227
MOD_SUMO_rev_2	386	394	PF00179	0.240
MOD_SUMO_rev_2	523	531	PF00179	0.185
TRG_DiLeu_BaEn_1	226	231	PF01217	0.345
TRG_ENDOCYTIC_2	113	116	PF00928	0.215
TRG_ENDOCYTIC_2	169	172	PF00928	0.345
TRG_ENDOCYTIC_2	206	209	PF00928	0.227
TRG_ENDOCYTIC_2	281	284	PF00928	0.227
TRG_ENDOCYTIC_2	475	478	PF00928	0.345
TRG_ENDOCYTIC_2	83	86	PF00928	0.242
TRG_ER_diArg_1	398	400	PF00400	0.370
TRG_ER_diArg_1	98	100	PF00400	0.231
TRG_Pf-PMV_PEXEL_1	100	104	PF00026	0.228
TRG_Pf-PMV_PEXEL_1	296	301	PF00026	0.189
TRG_Pf-PMV_PEXEL_1	393	397	PF00026	0.220
TRG_Pf-PMV_PEXEL_1	400	404	PF00026	0.198

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I631	Leptomonas seymouri	88%	100%
A0A0N1PCN7	Leptomonas seymouri	64%	100%
A0A1X0NIU7	Trypanosomatidae	63%	100%
A0A1X0NZZ8	Trypanosomatidae	74%	100%
A0A3R7N408	Trypanosoma rangeli	63%	100%
A0A3S5IRI9	Trypanosoma rangeli	72%	100%
A0A3S7WY69	Leishmania donovani	63%	100%
A2CIN0	Leishmania infantum	92%	100%
A2CIN8	Leishmania donovani	92%	100%
A4HD88	Leishmania braziliensis	61%	100%
C9ZLE3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	71%	100%
D0A701	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
E9AE57	Leishmania major	92%	100%
E9AH43	Leishmania infantum	63%	100%
E9AWM1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	61%	100%
P0AC33	Escherichia coli (strain K12)	64%	100%
P0AC34	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	64%	100%
P14407	Escherichia coli (strain K12)	64%	100%
P40720	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	66%	98%
Q04718	Geobacillus stearothermophilus	28%	100%
Q4QAU9	Leishmania major	62%	100%
Q8X4P8	Escherichia coli O157:H7	64%	100%
V5BGH8	Trypanosoma cruzi	73%	100%
V5DSN1	Trypanosoma cruzi	62%	100%