Non-specific serine/threonine protein kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RIO1 family, putative

Species:

Leishmania braziliensis

UniProt:

A4HH32_LEIBR

TriTrypDb:

LbrM.29.0250 , LBRM2903_290007800

Length:

538

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HH32

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HH32

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	5
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016310	phosphorylation	5	12
GO:0019538	protein metabolic process	3	5
GO:0036211	protein modification process	4	5
GO:0043170	macromolecule metabolic process	3	5
GO:0043412	macromolecule modification	4	5
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	5
GO:0071704	organic substance metabolic process	2	5
GO:1901564	organonitrogen compound metabolic process	3	5
GO:0022613	ribonucleoprotein complex biogenesis	4	1
GO:0042254	ribosome biogenesis	5	1
GO:0044085	cellular component biogenesis	3	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004672	protein kinase activity	3	12
GO:0004674	protein serine/threonine kinase activity	4	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140096	catalytic activity, acting on a protein	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0106310	protein serine kinase activity	4	4
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	398	402	PF00656	0.445
CLV_C14_Caspase3-7	465	469	PF00656	0.771
CLV_C14_Caspase3-7	483	487	PF00656	0.706
CLV_NRD_NRD_1	223	225	PF00675	0.195
CLV_NRD_NRD_1	296	298	PF00675	0.206
CLV_NRD_NRD_1	343	345	PF00675	0.195
CLV_NRD_NRD_1	506	508	PF00675	0.435
CLV_NRD_NRD_1	511	513	PF00675	0.434
CLV_NRD_NRD_1	521	523	PF00675	0.366
CLV_NRD_NRD_1	86	88	PF00675	0.405
CLV_PCSK_FUR_1	519	523	PF00082	0.437
CLV_PCSK_KEX2_1	343	345	PF00082	0.195
CLV_PCSK_KEX2_1	511	513	PF00082	0.426
CLV_PCSK_KEX2_1	521	523	PF00082	0.416
CLV_PCSK_KEX2_1	86	88	PF00082	0.486
CLV_PCSK_PC7_1	507	513	PF00082	0.355
CLV_PCSK_SKI1_1	118	122	PF00082	0.513
CLV_PCSK_SKI1_1	152	156	PF00082	0.307
CLV_PCSK_SKI1_1	2	6	PF00082	0.573
CLV_PCSK_SKI1_1	275	279	PF00082	0.262
CLV_PCSK_SKI1_1	489	493	PF00082	0.644
CLV_PCSK_SKI1_1	512	516	PF00082	0.427
CLV_TASPASE1	89	95	PF01112	0.406
DEG_APCC_DBOX_1	151	159	PF00400	0.301
DEG_Nend_UBRbox_1	1	4	PF02207	0.547
DEG_ODPH_VHL_1	247	260	PF01847	0.406
DOC_MAPK_gen_1	193	202	PF00069	0.395
DOC_MAPK_gen_1	233	241	PF00069	0.492
DOC_MAPK_gen_1	252	260	PF00069	0.321
DOC_MAPK_gen_1	343	349	PF00069	0.406
DOC_MAPK_MEF2A_6	417	426	PF00069	0.377
DOC_PP1_RVXF_1	273	280	PF00149	0.395
DOC_PP4_FxxP_1	36	39	PF00568	0.665
DOC_USP7_MATH_1	375	379	PF00917	0.428
DOC_USP7_MATH_1	65	69	PF00917	0.598
DOC_USP7_UBL2_3	283	287	PF12436	0.481
DOC_USP7_UBL2_3	504	508	PF12436	0.476
DOC_USP7_UBL2_3	527	531	PF12436	0.458
DOC_USP7_UBL2_3	533	537	PF12436	0.473
DOC_WW_Pin1_4	185	190	PF00397	0.462
DOC_WW_Pin1_4	484	489	PF00397	0.685
DOC_WW_Pin1_4	491	496	PF00397	0.592
LIG_14-3-3_CanoR_1	140	144	PF00244	0.458
LIG_APCC_ABBA_1	445	450	PF00400	0.513
LIG_BRCT_BRCA1_1	122	126	PF00533	0.610
LIG_BRCT_BRCA1_1	209	213	PF00533	0.462
LIG_BRCT_BRCA1_1	337	341	PF00533	0.481
LIG_eIF4E_1	300	306	PF01652	0.419
LIG_FHA_1	149	155	PF00498	0.323
LIG_FHA_1	195	201	PF00498	0.395
LIG_FHA_1	280	286	PF00498	0.438
LIG_FHA_2	16	22	PF00498	0.518
LIG_FHA_2	171	177	PF00498	0.338
LIG_FHA_2	227	233	PF00498	0.399
LIG_FHA_2	501	507	PF00498	0.456
LIG_LIR_Apic_2	266	272	PF02991	0.420
LIG_LIR_Apic_2	33	39	PF02991	0.671
LIG_LIR_Apic_2	330	336	PF02991	0.441
LIG_LIR_Gen_1	286	296	PF02991	0.395
LIG_LIR_Gen_1	311	318	PF02991	0.395
LIG_LIR_Gen_1	366	376	PF02991	0.325
LIG_LIR_Gen_1	420	429	PF02991	0.372
LIG_LIR_Nem_3	212	218	PF02991	0.424
LIG_LIR_Nem_3	286	292	PF02991	0.395
LIG_LIR_Nem_3	29	35	PF02991	0.570
LIG_LIR_Nem_3	311	316	PF02991	0.395
LIG_LIR_Nem_3	366	371	PF02991	0.261
LIG_LIR_Nem_3	420	426	PF02991	0.355
LIG_REV1ctd_RIR_1	350	358	PF16727	0.395
LIG_RPA_C_Insects	522	537	PF08784	0.536
LIG_SH2_CRK	156	160	PF00017	0.306
LIG_SH2_CRK	313	317	PF00017	0.230
LIG_SH2_SRC	333	336	PF00017	0.294
LIG_SH2_STAP1	32	36	PF00017	0.677
LIG_SH2_STAT5	208	211	PF00017	0.277
LIG_SH2_STAT5	218	221	PF00017	0.277
LIG_SH2_STAT5	289	292	PF00017	0.230
LIG_SH2_STAT5	35	38	PF00017	0.672
LIG_SH3_3	143	149	PF00018	0.394
LIG_SH3_3	243	249	PF00018	0.245
LIG_SH3_3	274	280	PF00018	0.230
LIG_SH3_3	45	51	PF00018	0.590
LIG_SUMO_SIM_par_1	321	326	PF11976	0.230
LIG_TRAF2_1	24	27	PF00917	0.559
LIG_TRAF2_1	503	506	PF00917	0.459
LIG_TYR_ITIM	154	159	PF00017	0.294
LIG_UBA3_1	392	397	PF00899	0.488
LIG_WRC_WIRS_1	199	204	PF05994	0.375
LIG_WRC_WIRS_1	349	354	PF05994	0.304
MOD_CDC14_SPxK_1	487	490	PF00782	0.626
MOD_CDK_SPK_2	484	489	PF00069	0.490
MOD_CDK_SPxK_1	484	490	PF00069	0.627
MOD_CK1_1	198	204	PF00069	0.399
MOD_CK1_1	529	535	PF00069	0.406
MOD_CK2_1	170	176	PF00069	0.345
MOD_CK2_1	21	27	PF00069	0.538
MOD_CK2_1	226	232	PF00069	0.235
MOD_CK2_1	428	434	PF00069	0.532
MOD_CK2_1	500	506	PF00069	0.494
MOD_Cter_Amidation	535	538	PF01082	0.669
MOD_GlcNHglycan	103	106	PF01048	0.517
MOD_GlcNHglycan	160	163	PF01048	0.335
MOD_GlcNHglycan	183	186	PF01048	0.375
MOD_GlcNHglycan	209	212	PF01048	0.323
MOD_GlcNHglycan	377	380	PF01048	0.434
MOD_GlcNHglycan	404	408	PF01048	0.344
MOD_GlcNHglycan	457	460	PF01048	0.721
MOD_GlcNHglycan	528	531	PF01048	0.456
MOD_GlcNHglycan	98	101	PF01048	0.557
MOD_GSK3_1	135	142	PF00069	0.511
MOD_GSK3_1	158	165	PF00069	0.447
MOD_GSK3_1	181	188	PF00069	0.384
MOD_GSK3_1	194	201	PF00069	0.424
MOD_GSK3_1	348	355	PF00069	0.374
MOD_GSK3_1	403	410	PF00069	0.344
MOD_GSK3_1	424	431	PF00069	0.500
MOD_N-GLC_1	424	429	PF02516	0.605
MOD_N-GLC_1	500	505	PF02516	0.602
MOD_N-GLC_2	168	170	PF02516	0.346
MOD_NEK2_1	120	125	PF00069	0.505
MOD_NEK2_1	158	163	PF00069	0.321
MOD_NEK2_1	226	231	PF00069	0.237
MOD_NEK2_1	292	297	PF00069	0.289
MOD_NEK2_1	352	357	PF00069	0.270
MOD_NEK2_1	424	429	PF00069	0.454
MOD_PIKK_1	325	331	PF00454	0.237
MOD_PIKK_1	352	358	PF00454	0.341
MOD_PKA_1	526	532	PF00069	0.491
MOD_PKA_2	139	145	PF00069	0.500
MOD_Plk_1	118	124	PF00069	0.465
MOD_Plk_1	424	430	PF00069	0.612
MOD_Plk_2-3	139	145	PF00069	0.472
MOD_Plk_2-3	477	483	PF00069	0.619
MOD_Plk_4	195	201	PF00069	0.274
MOD_Plk_4	348	354	PF00069	0.252
MOD_ProDKin_1	185	191	PF00069	0.323
MOD_ProDKin_1	484	490	PF00069	0.688
MOD_ProDKin_1	491	497	PF00069	0.586
MOD_SUMO_for_1	41	44	PF00179	0.583
TRG_DiLeu_BaEn_1	234	239	PF01217	0.375
TRG_DiLeu_BaEn_1	312	317	PF01217	0.289
TRG_DiLeu_BaEn_2	443	449	PF01217	0.469
TRG_DiLeu_BaLyEn_6	149	154	PF01217	0.339
TRG_ENDOCYTIC_2	156	159	PF00928	0.301
TRG_ENDOCYTIC_2	180	183	PF00928	0.230
TRG_ENDOCYTIC_2	289	292	PF00928	0.230
TRG_ENDOCYTIC_2	313	316	PF00928	0.230
TRG_ER_diArg_1	149	152	PF00400	0.354
TRG_ER_diArg_1	342	344	PF00400	0.230
TRG_ER_diArg_1	511	513	PF00400	0.358
TRG_ER_diArg_1	85	87	PF00400	0.424
TRG_ER_diLys_1	533	538	PF00400	0.554
TRG_NLS_Bipartite_1	521	538	PF00514	0.620
TRG_NLS_MonoExtC_3	532	537	PF00514	0.568
TRG_NLS_MonoExtN_4	488	493	PF00514	0.749
TRG_NLS_MonoExtN_4	531	538	PF00514	0.577
TRG_Pf-PMV_PEXEL_1	112	116	PF00026	0.497
TRG_Pf-PMV_PEXEL_1	430	434	PF00026	0.466

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIE8	Leptomonas seymouri	81%	97%
A0A0S4JDG4	Bodo saltans	53%	97%
A0A1X0NZG1	Trypanosomatidae	64%	97%
A0A3S7X229	Leishmania donovani	89%	100%
A0A422NUW2	Trypanosoma rangeli	62%	96%
A4I477	Leishmania infantum	89%	100%
C9ZKQ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	94%
E9ADM6	Leishmania major	89%	100%
E9AM55	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
G0S3J5	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	39%	100%
O42650	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	41%	100%
Q12196	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	100%
Q922Q2	Mus musculus	41%	95%
Q9BRS2	Homo sapiens	41%	95%
V5BUJ3	Trypanosoma cruzi	62%	99%