Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4HH14_LEIBR

TriTrypDb:

LbrM.29.0070 , LBRM2903_290006000

Length:

473

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HH14

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HH14

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006436	tryptophanyl-tRNA aminoacylation	7	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004830	tryptophan-tRNA ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	450	454	PF00656	0.297
CLV_C14_Caspase3-7	55	59	PF00656	0.405
CLV_NRD_NRD_1	133	135	PF00675	0.323
CLV_NRD_NRD_1	445	447	PF00675	0.407
CLV_PCSK_KEX2_1	133	135	PF00082	0.348
CLV_PCSK_KEX2_1	411	413	PF00082	0.300
CLV_PCSK_KEX2_1	447	449	PF00082	0.410
CLV_PCSK_PC1ET2_1	411	413	PF00082	0.300
CLV_PCSK_PC1ET2_1	447	449	PF00082	0.410
CLV_PCSK_SKI1_1	102	106	PF00082	0.423
CLV_PCSK_SKI1_1	134	138	PF00082	0.330
CLV_PCSK_SKI1_1	200	204	PF00082	0.285
CLV_PCSK_SKI1_1	274	278	PF00082	0.258
CLV_PCSK_SKI1_1	363	367	PF00082	0.250
CLV_PCSK_SKI1_1	371	375	PF00082	0.250
CLV_PCSK_SKI1_1	401	405	PF00082	0.336
CLV_PCSK_SKI1_1	421	425	PF00082	0.119
CLV_PCSK_SKI1_1	428	432	PF00082	0.245
CLV_PCSK_SKI1_1	447	451	PF00082	0.411
DOC_AGCK_PIF_2	235	240	PF00069	0.450
DOC_CKS1_1	88	93	PF01111	0.451
DOC_MAPK_gen_1	381	387	PF00069	0.555
DOC_PP1_RVXF_1	132	139	PF00149	0.331
DOC_PP2B_LxvP_1	4	7	PF13499	0.416
DOC_PP4_FxxP_1	187	190	PF00568	0.450
DOC_PP4_FxxP_1	327	330	PF00568	0.405
DOC_PP4_FxxP_1	9	12	PF00568	0.380
DOC_USP7_MATH_1	282	286	PF00917	0.450
DOC_USP7_MATH_1	469	473	PF00917	0.365
DOC_USP7_UBL2_3	200	204	PF12436	0.498
DOC_USP7_UBL2_3	411	415	PF12436	0.500
DOC_WW_Pin1_4	10	15	PF00397	0.369
DOC_WW_Pin1_4	358	363	PF00397	0.512
DOC_WW_Pin1_4	61	66	PF00397	0.407
DOC_WW_Pin1_4	87	92	PF00397	0.454
LIG_14-3-3_CanoR_1	162	171	PF00244	0.450
LIG_14-3-3_CanoR_1	446	456	PF00244	0.423
LIG_Actin_WH2_2	204	222	PF00022	0.536
LIG_Actin_WH2_2	314	331	PF00022	0.450
LIG_BRCT_BRCA1_1	12	16	PF00533	0.365
LIG_BRCT_BRCA1_1	286	290	PF00533	0.450
LIG_BRCT_BRCA1_1	334	338	PF00533	0.450
LIG_deltaCOP1_diTrp_1	58	67	PF00928	0.401
LIG_EH1_1	32	40	PF00400	0.329
LIG_eIF4E_1	33	39	PF01652	0.330
LIG_EVH1_1	4	8	PF00568	0.427
LIG_FHA_1	178	184	PF00498	0.448
LIG_FHA_1	214	220	PF00498	0.441
LIG_FHA_1	316	322	PF00498	0.449
LIG_FHA_1	34	40	PF00498	0.325
LIG_FHA_1	422	428	PF00498	0.457
LIG_FHA_1	434	440	PF00498	0.436
LIG_FHA_1	52	58	PF00498	0.350
LIG_FHA_2	194	200	PF00498	0.450
LIG_FHA_2	205	211	PF00498	0.501
LIG_FHA_2	389	395	PF00498	0.487
LIG_FHA_2	439	445	PF00498	0.382
LIG_FHA_2	448	454	PF00498	0.409
LIG_LIR_Apic_2	185	190	PF02991	0.450
LIG_LIR_Gen_1	237	245	PF02991	0.475
LIG_LIR_Gen_1	455	465	PF02991	0.508
LIG_LIR_Gen_1	75	83	PF02991	0.672
LIG_LIR_Gen_1	97	107	PF02991	0.413
LIG_LIR_Nem_3	13	19	PF02991	0.581
LIG_LIR_Nem_3	154	159	PF02991	0.474
LIG_LIR_Nem_3	232	238	PF02991	0.450
LIG_LIR_Nem_3	287	293	PF02991	0.445
LIG_LIR_Nem_3	312	317	PF02991	0.462
LIG_LIR_Nem_3	335	341	PF02991	0.442
LIG_LIR_Nem_3	455	460	PF02991	0.510
LIG_LIR_Nem_3	97	103	PF02991	0.415
LIG_MLH1_MIPbox_1	334	338	PF16413	0.450
LIG_Pex14_1	399	403	PF04695	0.450
LIG_Pex14_2	286	290	PF04695	0.450
LIG_Pex14_2	72	76	PF04695	0.518
LIG_REV1ctd_RIR_1	135	145	PF16727	0.332
LIG_REV1ctd_RIR_1	199	208	PF16727	0.491
LIG_SH2_CRK	100	104	PF00017	0.407
LIG_SH2_CRK	245	249	PF00017	0.450
LIG_SH2_STAP1	100	104	PF00017	0.396
LIG_SH2_STAT5	157	160	PF00017	0.450
LIG_SH2_STAT5	17	20	PF00017	0.355
LIG_SH2_STAT5	240	243	PF00017	0.511
LIG_SH2_STAT5	255	258	PF00017	0.536
LIG_SH2_STAT5	26	29	PF00017	0.569
LIG_SH2_STAT5	314	317	PF00017	0.448
LIG_SH2_STAT5	33	36	PF00017	0.396
LIG_SH3_2	5	10	PF14604	0.402
LIG_SH3_3	2	8	PF00018	0.431
LIG_SH3_3	390	396	PF00018	0.450
LIG_SUMO_SIM_anti_2	36	43	PF11976	0.331
LIG_SUMO_SIM_par_1	36	43	PF11976	0.331
LIG_TRAF2_1	207	210	PF00917	0.522
LIG_TRAF2_1	441	444	PF00917	0.458
LIG_TRAF2_1	83	86	PF00917	0.680
LIG_TYR_ITIM	98	103	PF00017	0.416
LIG_UBA3_1	174	181	PF00899	0.462
LIG_UBA3_1	293	298	PF00899	0.444
MOD_CDK_SPK_2	358	363	PF00069	0.536
MOD_CK1_1	165	171	PF00069	0.451
MOD_CK1_1	349	355	PF00069	0.462
MOD_CK2_1	193	199	PF00069	0.450
MOD_CK2_1	204	210	PF00069	0.499
MOD_CK2_1	438	444	PF00069	0.311
MOD_GlcNHglycan	286	289	PF01048	0.292
MOD_GlcNHglycan	341	344	PF01048	0.250
MOD_GlcNHglycan	374	377	PF01048	0.242
MOD_GlcNHglycan	42	45	PF01048	0.367
MOD_GSK3_1	433	440	PF00069	0.536
MOD_GSK3_1	47	54	PF00069	0.593
MOD_GSK3_1	56	63	PF00069	0.531
MOD_N-GLC_1	229	234	PF02516	0.263
MOD_NEK2_1	123	128	PF00069	0.264
MOD_NEK2_1	177	182	PF00069	0.450
MOD_NEK2_1	183	188	PF00069	0.481
MOD_NEK2_1	341	346	PF00069	0.465
MOD_NEK2_1	348	353	PF00069	0.410
MOD_NEK2_1	40	45	PF00069	0.352
MOD_NEK2_1	431	436	PF00069	0.406
MOD_NEK2_1	51	56	PF00069	0.366
MOD_NEK2_2	28	33	PF00069	0.354
MOD_PIKK_1	255	261	PF00454	0.450
MOD_PIKK_1	52	58	PF00454	0.410
MOD_PKA_1	447	453	PF00069	0.500
MOD_PKA_2	121	127	PF00069	0.502
MOD_PKA_2	315	321	PF00069	0.450
MOD_PKA_2	447	453	PF00069	0.453
MOD_Plk_1	204	210	PF00069	0.476
MOD_Plk_1	229	235	PF00069	0.371
MOD_Plk_1	28	34	PF00069	0.350
MOD_Plk_2-3	75	81	PF00069	0.396
MOD_Plk_4	229	235	PF00069	0.450
MOD_Plk_4	28	34	PF00069	0.499
MOD_ProDKin_1	10	16	PF00069	0.365
MOD_ProDKin_1	358	364	PF00069	0.512
MOD_ProDKin_1	61	67	PF00069	0.406
MOD_ProDKin_1	87	93	PF00069	0.455
MOD_SUMO_for_1	266	269	PF00179	0.462
MOD_SUMO_rev_2	197	202	PF00179	0.450
MOD_SUMO_rev_2	405	413	PF00179	0.533
TRG_DiLeu_BaEn_4	408	414	PF01217	0.536
TRG_ENDOCYTIC_2	100	103	PF00928	0.415
TRG_ENDOCYTIC_2	245	248	PF00928	0.450
TRG_ENDOCYTIC_2	314	317	PF00928	0.450
TRG_ER_diArg_1	132	134	PF00400	0.329
TRG_ER_diArg_1	446	449	PF00400	0.307
TRG_NLS_Bipartite_1	428	450	PF00514	0.586
TRG_NLS_MonoExtC_3	445	451	PF00514	0.496

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	84%	100%
A0A0N1HXL2	Leptomonas seymouri	41%	100%
A0A0S4IN16	Bodo saltans	75%	100%
A0A0S4JC70	Bodo saltans	43%	100%
A0A1X0NGJ6	Trypanosomatidae	40%	100%
A0A1X0NZB4	Trypanosomatidae	76%	100%
A0A3R7MPN7	Trypanosoma rangeli	78%	100%
A0A3S7WXL2	Leishmania donovani	45%	100%
A0A3S7X238	Leishmania donovani	90%	100%
A0A422NW64	Trypanosoma rangeli	41%	100%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	30%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	38%	100%
A4HCP4	Leishmania braziliensis	38%	100%
A4I070	Leishmania infantum	44%	100%
A4I443	Leishmania infantum	90%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	35%	100%
B6YUH1	Thermococcus onnurineus (strain NA1)	45%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	45%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	78%	100%
E9ADK8	Leishmania major	88%	100%
E9AM73	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AW33	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	31%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	45%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	43%	100%
P17248	Bos taurus	54%	99%
P23381	Homo sapiens	55%	100%
P23612	Oryctolagus cuniculus	55%	100%
P32921	Mus musculus	55%	98%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	55%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	48%	100%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	46%	100%
Q4QBE4	Leishmania major	43%	100%
Q55DZ8	Dictyostelium discoideum	54%	100%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	28%	100%
Q5JEP3	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	44%	100%
Q5R4J1	Pongo abelii	55%	100%
Q5V4J1	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	26%	100%
Q6P7B0	Rattus norvegicus	55%	98%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	30%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	45%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	35%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	46%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	48%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	35%	100%
Q9SR15	Arabidopsis thaliana	57%	100%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	45%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	31%	100%
V5B4S5	Trypanosoma cruzi	79%	100%