ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair and recombination helicase protein PIF3, putative

Species:

Leishmania braziliensis

UniProt:

A4HGV5_LEIBR

TriTrypDb:

LbrM.28.2730 , LBRM2903_280034200

Length:

859

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005657	replication fork	2	1

Expansion

Sequence features

A4HGV5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HGV5

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006310	DNA recombination	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0032200	telomere organization	6	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051276	chromosome organization	5	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006260	DNA replication	5	1
GO:0032392	DNA geometric change	7	1
GO:0032508	DNA duplex unwinding	8	1
GO:0071103	DNA conformation change	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003678	DNA helicase activity	3	11
GO:0003824	catalytic activity	1	11
GO:0004386	helicase activity	2	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008094	ATP-dependent activity, acting on DNA	2	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0016887	ATP hydrolysis activity	7	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000287	magnesium ion binding	5	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	298	302	PF00656	0.335
CLV_NRD_NRD_1	408	410	PF00675	0.459
CLV_NRD_NRD_1	44	46	PF00675	0.458
CLV_NRD_NRD_1	483	485	PF00675	0.756
CLV_NRD_NRD_1	581	583	PF00675	0.696
CLV_NRD_NRD_1	593	595	PF00675	0.600
CLV_NRD_NRD_1	693	695	PF00675	0.516
CLV_NRD_NRD_1	77	79	PF00675	0.646
CLV_NRD_NRD_1	794	796	PF00675	0.525
CLV_NRD_NRD_1	813	815	PF00675	0.361
CLV_NRD_NRD_1	87	89	PF00675	0.668
CLV_NRD_NRD_1	96	98	PF00675	0.404
CLV_PCSK_FUR_1	591	595	PF00082	0.704
CLV_PCSK_KEX2_1	407	409	PF00082	0.478
CLV_PCSK_KEX2_1	581	583	PF00082	0.682
CLV_PCSK_KEX2_1	590	592	PF00082	0.694
CLV_PCSK_KEX2_1	593	595	PF00082	0.646
CLV_PCSK_KEX2_1	693	695	PF00082	0.516
CLV_PCSK_KEX2_1	77	79	PF00082	0.672
CLV_PCSK_KEX2_1	813	815	PF00082	0.489
CLV_PCSK_KEX2_1	87	89	PF00082	0.625
CLV_PCSK_PC1ET2_1	590	592	PF00082	0.731
CLV_PCSK_SKI1_1	143	147	PF00082	0.622
CLV_PCSK_SKI1_1	2	6	PF00082	0.639
CLV_PCSK_SKI1_1	212	216	PF00082	0.342
CLV_PCSK_SKI1_1	230	234	PF00082	0.311
CLV_PCSK_SKI1_1	357	361	PF00082	0.438
CLV_PCSK_SKI1_1	515	519	PF00082	0.465
CLV_PCSK_SKI1_1	582	586	PF00082	0.539
CLV_PCSK_SKI1_1	618	622	PF00082	0.503
CLV_PCSK_SKI1_1	700	704	PF00082	0.571
CLV_PCSK_SKI1_1	814	818	PF00082	0.479
CLV_PCSK_SKI1_1	852	856	PF00082	0.420
CLV_PCSK_SKI1_1	97	101	PF00082	0.747
DEG_APCC_DBOX_1	463	471	PF00400	0.527
DEG_Kelch_Keap1_1	539	544	PF01344	0.530
DEG_ODPH_VHL_1	492	504	PF01847	0.372
DEG_ODPH_VHL_1	651	664	PF01847	0.432
DEG_SCF_TRCP1_1	541	547	PF00400	0.529
DOC_CKS1_1	190	195	PF01111	0.628
DOC_CKS1_1	358	363	PF01111	0.510
DOC_CKS1_1	99	104	PF01111	0.488
DOC_CYCLIN_RxL_1	91	104	PF00134	0.459
DOC_CYCLIN_yCln2_LP_2	358	364	PF00134	0.510
DOC_CYCLIN_yCln2_LP_2	390	396	PF00134	0.586
DOC_CYCLIN_yCln2_LP_2	749	755	PF00134	0.515
DOC_MAPK_DCC_7	710	720	PF00069	0.510
DOC_MAPK_gen_1	45	53	PF00069	0.474
DOC_MAPK_gen_1	655	664	PF00069	0.382
DOC_MAPK_gen_1	793	802	PF00069	0.384
DOC_MAPK_gen_1	813	819	PF00069	0.220
DOC_MAPK_MEF2A_6	208	215	PF00069	0.252
DOC_MAPK_MEF2A_6	239	248	PF00069	0.285
DOC_MAPK_RevD_3	575	591	PF00069	0.449
DOC_MAPK_RevD_3	800	814	PF00069	0.436
DOC_PP1_RVXF_1	850	856	PF00149	0.425
DOC_PP2B_LxvP_1	749	752	PF13499	0.516
DOC_PP4_FxxP_1	19	22	PF00568	0.537
DOC_PP4_FxxP_1	270	273	PF00568	0.335
DOC_PP4_FxxP_1	683	686	PF00568	0.414
DOC_PP4_FxxP_1	855	858	PF00568	0.452
DOC_USP7_MATH_1	118	122	PF00917	0.651
DOC_USP7_MATH_1	133	137	PF00917	0.414
DOC_USP7_MATH_1	344	348	PF00917	0.475
DOC_USP7_MATH_1	395	399	PF00917	0.356
DOC_USP7_MATH_1	566	570	PF00917	0.500
DOC_USP7_MATH_1	839	843	PF00917	0.514
DOC_USP7_UBL2_3	115	119	PF12436	0.500
DOC_USP7_UBL2_3	91	95	PF12436	0.427
DOC_WW_Pin1_4	189	194	PF00397	0.570
DOC_WW_Pin1_4	357	362	PF00397	0.499
DOC_WW_Pin1_4	427	432	PF00397	0.481
DOC_WW_Pin1_4	53	58	PF00397	0.619
DOC_WW_Pin1_4	854	859	PF00397	0.561
DOC_WW_Pin1_4	98	103	PF00397	0.480
LIG_14-3-3_CanoR_1	180	188	PF00244	0.533
LIG_14-3-3_CanoR_1	247	256	PF00244	0.368
LIG_14-3-3_CanoR_1	582	588	PF00244	0.608
LIG_14-3-3_CanoR_1	618	623	PF00244	0.486
LIG_14-3-3_CanoR_1	813	819	PF00244	0.517
LIG_14-3-3_CanoR_1	840	844	PF00244	0.461
LIG_14-3-3_CanoR_1	852	858	PF00244	0.574
LIG_Actin_WH2_2	234	249	PF00022	0.335
LIG_Actin_WH2_2	60	76	PF00022	0.355
LIG_Actin_WH2_2	644	659	PF00022	0.536
LIG_BIR_III_2	275	279	PF00653	0.211
LIG_BIR_III_4	453	457	PF00653	0.641
LIG_BRCT_BRCA1_1	11	15	PF00533	0.618
LIG_BRCT_BRCA1_1	346	350	PF00533	0.400
LIG_BRCT_BRCA1_1	39	43	PF00533	0.441
LIG_EH_1	20	24	PF12763	0.545
LIG_FHA_1	229	235	PF00498	0.335
LIG_FHA_1	251	257	PF00498	0.335
LIG_FHA_1	28	34	PF00498	0.494
LIG_FHA_1	349	355	PF00498	0.260
LIG_FHA_1	5	11	PF00498	0.636
LIG_FHA_1	590	596	PF00498	0.684
LIG_FHA_1	634	640	PF00498	0.600
LIG_FHA_1	748	754	PF00498	0.517
LIG_FHA_1	770	776	PF00498	0.378
LIG_FHA_1	94	100	PF00498	0.635
LIG_FHA_2	190	196	PF00498	0.543
LIG_FHA_2	421	427	PF00498	0.346
LIG_FHA_2	619	625	PF00498	0.486
LIG_FHA_2	641	647	PF00498	0.605
LIG_Integrin_isoDGR_2	24	26	PF01839	0.628
LIG_LIR_Apic_2	17	22	PF02991	0.531
LIG_LIR_Apic_2	182	188	PF02991	0.466
LIG_LIR_Apic_2	402	406	PF02991	0.413
LIG_LIR_Apic_2	681	686	PF02991	0.415
LIG_LIR_Gen_1	165	174	PF02991	0.621
LIG_LIR_Gen_1	175	186	PF02991	0.645
LIG_LIR_Gen_1	216	226	PF02991	0.211
LIG_LIR_Gen_1	332	340	PF02991	0.335
LIG_LIR_Gen_1	347	358	PF02991	0.390
LIG_LIR_Gen_1	384	395	PF02991	0.523
LIG_LIR_Gen_1	603	612	PF02991	0.411
LIG_LIR_LC3C_4	798	802	PF02991	0.482
LIG_LIR_Nem_3	125	130	PF02991	0.421
LIG_LIR_Nem_3	165	170	PF02991	0.569
LIG_LIR_Nem_3	175	181	PF02991	0.595
LIG_LIR_Nem_3	216	221	PF02991	0.211
LIG_LIR_Nem_3	332	336	PF02991	0.328
LIG_LIR_Nem_3	347	353	PF02991	0.393
LIG_LIR_Nem_3	384	390	PF02991	0.519
LIG_LIR_Nem_3	603	607	PF02991	0.423
LIG_OCRL_FandH_1	166	178	PF00620	0.390
LIG_PCNA_yPIPBox_3	710	721	PF02747	0.543
LIG_PDZ_Class_1	854	859	PF00595	0.451
LIG_Pex14_2	15	19	PF04695	0.534
LIG_Pex14_2	266	270	PF04695	0.321
LIG_SH2_CRK	11	15	PF00017	0.650
LIG_SH2_CRK	178	182	PF00017	0.651
LIG_SH2_CRK	185	189	PF00017	0.625
LIG_SH2_CRK	44	48	PF00017	0.607
LIG_SH2_GRB2like	11	14	PF00017	0.393
LIG_SH2_NCK_1	345	349	PF00017	0.509
LIG_SH2_STAP1	11	15	PF00017	0.501
LIG_SH2_STAP1	345	349	PF00017	0.516
LIG_SH2_STAP1	757	761	PF00017	0.472
LIG_SH2_STAT3	803	806	PF00017	0.557
LIG_SH2_STAT5	169	172	PF00017	0.587
LIG_SH2_STAT5	236	239	PF00017	0.252
LIG_SH2_STAT5	403	406	PF00017	0.413
LIG_SH2_STAT5	742	745	PF00017	0.470
LIG_SH2_STAT5	788	791	PF00017	0.353
LIG_SH3_3	190	196	PF00018	0.669
LIG_SH3_3	44	50	PF00018	0.561
LIG_SH3_3	499	505	PF00018	0.441
LIG_SH3_3	560	566	PF00018	0.504
LIG_SH3_3	723	729	PF00018	0.417
LIG_SH3_3	829	835	PF00018	0.374
LIG_SUMO_SIM_anti_2	290	296	PF11976	0.331
LIG_SUMO_SIM_anti_2	500	505	PF11976	0.548
LIG_SUMO_SIM_par_1	290	296	PF11976	0.294
LIG_SUMO_SIM_par_1	631	636	PF11976	0.454
LIG_SUMO_SIM_par_1	729	734	PF11976	0.293
LIG_SUMO_SIM_par_1	771	776	PF11976	0.367
LIG_TRAF2_1	423	426	PF00917	0.358
LIG_TRAF2_1	83	86	PF00917	0.455
MOD_CDK_SPxK_1	357	363	PF00069	0.506
MOD_CDK_SPxK_1	427	433	PF00069	0.535
MOD_CDK_SPxxK_3	98	105	PF00069	0.488
MOD_CK1_1	136	142	PF00069	0.458
MOD_CK1_1	179	185	PF00069	0.544
MOD_CK1_1	225	231	PF00069	0.378
MOD_CK1_1	420	426	PF00069	0.488
MOD_CK1_1	539	545	PF00069	0.715
MOD_CK1_1	552	558	PF00069	0.721
MOD_CK1_1	576	582	PF00069	0.718
MOD_CK1_1	673	679	PF00069	0.426
MOD_CK1_1	841	847	PF00069	0.533
MOD_CK2_1	420	426	PF00069	0.351
MOD_CK2_1	53	59	PF00069	0.616
MOD_CK2_1	597	603	PF00069	0.518
MOD_CK2_1	618	624	PF00069	0.526
MOD_CK2_1	812	818	PF00069	0.410
MOD_GlcNHglycan	116	119	PF01048	0.764
MOD_GlcNHglycan	120	123	PF01048	0.676
MOD_GlcNHglycan	135	138	PF01048	0.307
MOD_GlcNHglycan	181	184	PF01048	0.445
MOD_GlcNHglycan	215	218	PF01048	0.401
MOD_GlcNHglycan	224	227	PF01048	0.371
MOD_GlcNHglycan	346	349	PF01048	0.391
MOD_GlcNHglycan	444	447	PF01048	0.515
MOD_GlcNHglycan	529	532	PF01048	0.724
MOD_GlcNHglycan	538	541	PF01048	0.750
MOD_GlcNHglycan	554	557	PF01048	0.629
MOD_GlcNHglycan	568	571	PF01048	0.684
MOD_GlcNHglycan	599	602	PF01048	0.545
MOD_GlcNHglycan	68	71	PF01048	0.345
MOD_GlcNHglycan	696	700	PF01048	0.523
MOD_GlcNHglycan	814	817	PF01048	0.405
MOD_GSK3_1	114	121	PF00069	0.713
MOD_GSK3_1	132	139	PF00069	0.548
MOD_GSK3_1	191	198	PF00069	0.568
MOD_GSK3_1	25	32	PF00069	0.544
MOD_GSK3_1	250	257	PF00069	0.244
MOD_GSK3_1	334	341	PF00069	0.473
MOD_GSK3_1	344	351	PF00069	0.432
MOD_GSK3_1	395	402	PF00069	0.542
MOD_GSK3_1	417	424	PF00069	0.408
MOD_GSK3_1	438	445	PF00069	0.577
MOD_GSK3_1	545	552	PF00069	0.719
MOD_GSK3_1	582	589	PF00069	0.579
MOD_GSK3_1	606	613	PF00069	0.483
MOD_GSK3_1	666	673	PF00069	0.450
MOD_GSK3_1	731	738	PF00069	0.308
MOD_GSK3_1	765	772	PF00069	0.402
MOD_GSK3_1	834	841	PF00069	0.441
MOD_LATS_1	379	385	PF00433	0.304
MOD_N-GLC_1	396	401	PF02516	0.526
MOD_N-GLC_1	666	671	PF02516	0.476
MOD_N-GLC_1	673	678	PF02516	0.427
MOD_NEK2_1	15	20	PF00069	0.628
MOD_NEK2_1	213	218	PF00069	0.279
MOD_NEK2_1	250	255	PF00069	0.335
MOD_NEK2_1	27	32	PF00069	0.535
MOD_NEK2_1	37	42	PF00069	0.469
MOD_NEK2_1	438	443	PF00069	0.481
MOD_NEK2_1	633	638	PF00069	0.526
MOD_NEK2_1	668	673	PF00069	0.449
MOD_NEK2_1	735	740	PF00069	0.590
MOD_NEK2_1	769	774	PF00069	0.385
MOD_NEK2_1	817	822	PF00069	0.349
MOD_NEK2_2	262	267	PF00069	0.354
MOD_NEK2_2	757	762	PF00069	0.376
MOD_PIKK_1	136	142	PF00454	0.612
MOD_PIKK_1	201	207	PF00454	0.211
MOD_PIKK_1	239	245	PF00454	0.211
MOD_PIKK_1	421	427	PF00454	0.572
MOD_PIKK_1	472	478	PF00454	0.341
MOD_PIKK_1	546	552	PF00454	0.524
MOD_PIKK_1	841	847	PF00454	0.500
MOD_PKA_1	45	51	PF00069	0.395
MOD_PKA_1	87	93	PF00069	0.446
MOD_PKA_2	104	110	PF00069	0.633
MOD_PKA_2	179	185	PF00069	0.685
MOD_PKA_2	25	31	PF00069	0.544
MOD_PKA_2	692	698	PF00069	0.650
MOD_PKA_2	812	818	PF00069	0.478
MOD_PKA_2	839	845	PF00069	0.411
MOD_PKA_2	87	93	PF00069	0.539
MOD_Plk_1	239	245	PF00069	0.329
MOD_Plk_1	295	301	PF00069	0.335
MOD_Plk_1	338	344	PF00069	0.486
MOD_Plk_1	396	402	PF00069	0.481
MOD_Plk_1	425	431	PF00069	0.404
MOD_Plk_2-3	295	301	PF00069	0.335
MOD_Plk_4	15	21	PF00069	0.596
MOD_Plk_4	228	234	PF00069	0.373
MOD_Plk_4	295	301	PF00069	0.335
MOD_Plk_4	399	405	PF00069	0.321
MOD_Plk_4	549	555	PF00069	0.545
MOD_Plk_4	618	624	PF00069	0.484
MOD_Plk_4	626	632	PF00069	0.427
MOD_Plk_4	738	744	PF00069	0.430
MOD_Plk_4	769	775	PF00069	0.380
MOD_Plk_4	805	811	PF00069	0.522
MOD_ProDKin_1	189	195	PF00069	0.571
MOD_ProDKin_1	357	363	PF00069	0.506
MOD_ProDKin_1	427	433	PF00069	0.488
MOD_ProDKin_1	53	59	PF00069	0.616
MOD_ProDKin_1	98	104	PF00069	0.485
MOD_SUMO_rev_2	410	420	PF00179	0.437
TRG_DiLeu_BaEn_3	625	631	PF01217	0.508
TRG_DiLeu_BaLyEn_6	369	374	PF01217	0.435
TRG_DiLeu_BaLyEn_6	463	468	PF01217	0.648
TRG_DiLeu_BaLyEn_6	716	721	PF01217	0.441
TRG_ENDOCYTIC_2	11	14	PF00928	0.628
TRG_ENDOCYTIC_2	178	181	PF00928	0.545
TRG_ENDOCYTIC_2	238	241	PF00928	0.479
TRG_ENDOCYTIC_2	44	47	PF00928	0.625
TRG_ER_diArg_1	406	409	PF00400	0.461
TRG_ER_diArg_1	518	521	PF00400	0.412
TRG_ER_diArg_1	580	582	PF00400	0.693
TRG_ER_diArg_1	591	594	PF00400	0.682
TRG_ER_diArg_1	692	694	PF00400	0.517
TRG_ER_diArg_1	812	814	PF00400	0.574
TRG_ER_diArg_1	87	89	PF00400	0.562
TRG_NLS_MonoExtC_3	589	594	PF00514	0.709
TRG_Pf-PMV_PEXEL_1	239	243	PF00026	0.304
TRG_Pf-PMV_PEXEL_1	372	376	PF00026	0.415
TRG_Pf-PMV_PEXEL_1	719	724	PF00026	0.425
TRG_Pf-PMV_PEXEL_1	795	799	PF00026	0.457

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCJ6	Leptomonas seymouri	59%	77%
A0A1X0NSB2	Trypanosomatidae	50%	100%
A0A3Q8IGY7	Leishmania donovani	80%	92%
A0A422NKI2	Trypanosoma rangeli	49%	100%
A4I3Y5	Leishmania infantum	80%	92%
D0A956	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9B075	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	92%
Q383A1	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	46%	100%
Q4Q810	Leishmania major	79%	100%
V5BL79	Trypanosoma cruzi	50%	100%