Protein RFT1 homolog

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dolichyl-lipid chaperone, putative

Species:

Leishmania braziliensis

UniProt:

A4HGU4_LEIBR

TriTrypDb:

LbrM.28.2610 , LBRM2903_280032800

Length:

729

Annotations

LeishMANIAdb annotations

Highly conserved Eukaryotic protein. Involved in N-linked oligosaccharide assembly.. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005635	nuclear envelope	4	1
GO:0005783	endoplasmic reticulum	5	1
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0031090	organelle membrane	3	1
GO:0031967	organelle envelope	3	1
GO:0031975	envelope	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4HGU4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HGU4

Function

Biological processes
Term	Name	Level	Count
GO:0006488	dolichol-linked oligosaccharide biosynthetic process	5	11
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	11
GO:0006629	lipid metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009987	cellular process	1	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044255	cellular lipid metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:1901135	carbohydrate derivative metabolic process	3	11
GO:1901137	carbohydrate derivative biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0016043	cellular component organization	3	1
GO:0034203	glycolipid translocation	5	1
GO:0034204	lipid translocation	4	1
GO:0046836	glycolipid transport	6	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0061024	membrane organization	4	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0071702	organic substance transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0097035	regulation of membrane lipid distribution	3	1
GO:1901264	carbohydrate derivative transport	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	180	184	PF00656	0.417
CLV_C14_Caspase3-7	373	377	PF00656	0.539
CLV_NRD_NRD_1	382	384	PF00675	0.282
CLV_NRD_NRD_1	687	689	PF00675	0.510
CLV_PCSK_KEX2_1	382	384	PF00082	0.282
CLV_PCSK_KEX2_1	644	646	PF00082	0.331
CLV_PCSK_KEX2_1	687	689	PF00082	0.491
CLV_PCSK_PC1ET2_1	644	646	PF00082	0.251
CLV_PCSK_SKI1_1	24	28	PF00082	0.289
CLV_PCSK_SKI1_1	36	40	PF00082	0.285
CLV_PCSK_SKI1_1	398	402	PF00082	0.257
CLV_PCSK_SKI1_1	433	437	PF00082	0.236
CLV_PCSK_SKI1_1	464	468	PF00082	0.282
CLV_PCSK_SKI1_1	487	491	PF00082	0.511
CLV_PCSK_SKI1_1	496	500	PF00082	0.490
CLV_PCSK_SKI1_1	61	65	PF00082	0.363
CLV_PCSK_SKI1_1	687	691	PF00082	0.574
CLV_PCSK_SKI1_1	699	703	PF00082	0.602
DEG_APCC_DBOX_1	392	400	PF00400	0.531
DEG_APCC_DBOX_1	696	704	PF00400	0.394
DEG_MDM2_SWIB_1	579	587	PF02201	0.335
DEG_SCF_FBW7_1	352	359	PF00400	0.381
DEG_SCF_FBW7_1	689	696	PF00400	0.373
DOC_CKS1_1	353	358	PF01111	0.382
DOC_CKS1_1	497	502	PF01111	0.331
DOC_CKS1_1	690	695	PF01111	0.423
DOC_CYCLIN_RxL_1	430	440	PF00134	0.531
DOC_MAPK_FxFP_2	365	368	PF00069	0.531
DOC_MAPK_gen_1	658	668	PF00069	0.572
DOC_MAPK_MEF2A_6	10	19	PF00069	0.331
DOC_MAPK_MEF2A_6	126	133	PF00069	0.531
DOC_MAPK_MEF2A_6	393	401	PF00069	0.478
DOC_MAPK_MEF2A_6	464	471	PF00069	0.504
DOC_MAPK_MEF2A_6	584	593	PF00069	0.289
DOC_MAPK_NFAT4_5	10	18	PF00069	0.331
DOC_MAPK_NFAT4_5	464	472	PF00069	0.482
DOC_MAPK_RevD_3	631	645	PF00069	0.478
DOC_PP1_RVXF_1	22	29	PF00149	0.289
DOC_PP1_RVXF_1	431	437	PF00149	0.436
DOC_PP1_RVXF_1	466	472	PF00149	0.349
DOC_PP2B_LxvP_1	37	40	PF13499	0.513
DOC_PP2B_LxvP_1	480	483	PF13499	0.291
DOC_PP4_FxxP_1	365	368	PF00568	0.531
DOC_USP7_MATH_1	132	136	PF00917	0.295
DOC_USP7_MATH_1	166	170	PF00917	0.416
DOC_USP7_MATH_1	336	340	PF00917	0.590
DOC_USP7_MATH_1	354	358	PF00917	0.373
DOC_USP7_MATH_1	500	504	PF00917	0.282
DOC_USP7_MATH_1	622	626	PF00917	0.715
DOC_USP7_MATH_1	63	67	PF00917	0.510
DOC_USP7_MATH_1	714	718	PF00917	0.436
DOC_WW_Pin1_4	112	117	PF00397	0.582
DOC_WW_Pin1_4	308	313	PF00397	0.532
DOC_WW_Pin1_4	352	357	PF00397	0.480
DOC_WW_Pin1_4	496	501	PF00397	0.267
DOC_WW_Pin1_4	528	533	PF00397	0.556
DOC_WW_Pin1_4	592	597	PF00397	0.528
DOC_WW_Pin1_4	624	629	PF00397	0.693
DOC_WW_Pin1_4	689	694	PF00397	0.419
LIG_14-3-3_CanoR_1	114	120	PF00244	0.600
LIG_14-3-3_CanoR_1	167	174	PF00244	0.468
LIG_14-3-3_CanoR_1	260	266	PF00244	0.394
LIG_14-3-3_CanoR_1	361	366	PF00244	0.499
LIG_14-3-3_CanoR_1	398	408	PF00244	0.454
LIG_14-3-3_CanoR_1	456	463	PF00244	0.480
LIG_14-3-3_CanoR_1	661	666	PF00244	0.414
LIG_Actin_WH2_2	10	26	PF00022	0.246
LIG_Actin_WH2_2	385	400	PF00022	0.492
LIG_APCC_ABBA_1	49	54	PF00400	0.506
LIG_BRCT_BRCA1_1	363	367	PF00533	0.462
LIG_eIF4E_1	425	431	PF01652	0.446
LIG_eIF4E_1	52	58	PF01652	0.477
LIG_FHA_1	201	207	PF00498	0.458
LIG_FHA_1	25	31	PF00498	0.275
LIG_FHA_1	288	294	PF00498	0.356
LIG_FHA_1	33	39	PF00498	0.327
LIG_FHA_1	46	52	PF00498	0.452
LIG_FHA_1	497	503	PF00498	0.311
LIG_FHA_1	595	601	PF00498	0.487
LIG_FHA_1	62	68	PF00498	0.533
LIG_FHA_2	259	265	PF00498	0.348
LIG_FHA_2	270	276	PF00498	0.322
LIG_GBD_Chelix_1	15	23	PF00786	0.404
LIG_LIR_Apic_2	363	368	PF02991	0.529
LIG_LIR_Apic_2	494	500	PF02991	0.267
LIG_LIR_Gen_1	359	369	PF02991	0.501
LIG_LIR_Gen_1	476	484	PF02991	0.421
LIG_LIR_Gen_1	588	598	PF02991	0.425
LIG_LIR_Gen_1	637	646	PF02991	0.553
LIG_LIR_Gen_1	692	703	PF02991	0.383
LIG_LIR_LC3C_4	296	299	PF02991	0.193
LIG_LIR_LC3C_4	429	432	PF02991	0.381
LIG_LIR_Nem_3	225	230	PF02991	0.387
LIG_LIR_Nem_3	359	365	PF02991	0.465
LIG_LIR_Nem_3	476	480	PF02991	0.406
LIG_LIR_Nem_3	48	52	PF02991	0.485
LIG_LIR_Nem_3	588	593	PF02991	0.351
LIG_LIR_Nem_3	637	641	PF02991	0.580
LIG_LIR_Nem_3	692	698	PF02991	0.337
LIG_MYND_1	624	628	PF01753	0.633
LIG_NRBOX	29	35	PF00104	0.276
LIG_PCNA_yPIPBox_3	519	527	PF02747	0.349
LIG_Pex14_2	365	369	PF04695	0.531
LIG_Pex14_2	471	475	PF04695	0.404
LIG_Pex14_2	579	583	PF04695	0.335
LIG_Pex14_2	638	642	PF04695	0.535
LIG_PTAP_UEV_1	107	112	PF05743	0.682
LIG_PTB_Apo_2	40	47	PF02174	0.523
LIG_PTB_Phospho_1	40	46	PF10480	0.523
LIG_SH2_CRK	295	299	PF00017	0.394
LIG_SH2_CRK	421	425	PF00017	0.436
LIG_SH2_CRK	511	515	PF00017	0.276
LIG_SH2_GRB2like	41	44	PF00017	0.494
LIG_SH2_NCK_1	165	169	PF00017	0.259
LIG_SH2_PTP2	554	557	PF00017	0.314
LIG_SH2_SRC	41	44	PF00017	0.554
LIG_SH2_STAP1	295	299	PF00017	0.394
LIG_SH2_STAP1	493	497	PF00017	0.321
LIG_SH2_STAP1	675	679	PF00017	0.335
LIG_SH2_STAT3	250	253	PF00017	0.331
LIG_SH2_STAT5	198	201	PF00017	0.275
LIG_SH2_STAT5	295	298	PF00017	0.218
LIG_SH2_STAT5	46	49	PF00017	0.472
LIG_SH2_STAT5	472	475	PF00017	0.295
LIG_SH2_STAT5	493	496	PF00017	0.299
LIG_SH2_STAT5	509	512	PF00017	0.189
LIG_SH2_STAT5	549	552	PF00017	0.277
LIG_SH2_STAT5	554	557	PF00017	0.264
LIG_SH2_STAT5	590	593	PF00017	0.452
LIG_SH3_3	105	111	PF00018	0.689
LIG_SH3_3	191	197	PF00018	0.357
LIG_SH3_3	526	532	PF00018	0.584
LIG_SH3_3	590	596	PF00018	0.383
LIG_SH3_3	612	618	PF00018	0.784
LIG_SH3_3	625	631	PF00018	0.566
LIG_Sin3_3	319	326	PF02671	0.482
LIG_SUMO_SIM_anti_2	568	574	PF11976	0.333
LIG_SUMO_SIM_anti_2	664	670	PF11976	0.193
LIG_SUMO_SIM_par_1	201	208	PF11976	0.245
LIG_SUMO_SIM_par_1	217	223	PF11976	0.329
LIG_SUMO_SIM_par_1	228	234	PF11976	0.472
LIG_SUMO_SIM_par_1	295	300	PF11976	0.215
LIG_TRAF2_1	92	95	PF00917	0.602
LIG_TYR_ITIM	292	297	PF00017	0.404
LIG_WRC_WIRS_1	25	30	PF05994	0.288
LIG_WRC_WIRS_1	388	393	PF05994	0.335
LIG_WRC_WIRS_1	46	51	PF05994	0.359
MOD_CDK_SPxxK_3	627	634	PF00069	0.353
MOD_CK1_1	109	115	PF00069	0.679
MOD_CK1_1	29	35	PF00069	0.404
MOD_CK1_1	341	347	PF00069	0.289
MOD_CK1_1	4	10	PF00069	0.393
MOD_CK1_1	411	417	PF00069	0.358
MOD_CK1_1	594	600	PF00069	0.326
MOD_CK1_1	66	72	PF00069	0.335
MOD_CK1_1	717	723	PF00069	0.521
MOD_CK2_1	259	265	PF00069	0.423
MOD_CK2_1	269	275	PF00069	0.393
MOD_CK2_1	307	313	PF00069	0.402
MOD_CK2_1	399	405	PF00069	0.289
MOD_CK2_1	723	729	PF00069	0.439
MOD_CK2_1	73	79	PF00069	0.363
MOD_GlcNHglycan	108	111	PF01048	0.701
MOD_GlcNHglycan	183	186	PF01048	0.481
MOD_GlcNHglycan	299	302	PF01048	0.427
MOD_GlcNHglycan	338	341	PF01048	0.433
MOD_GlcNHglycan	343	346	PF01048	0.410
MOD_GlcNHglycan	722	725	PF01048	0.685
MOD_GlcNHglycan	75	79	PF01048	0.570
MOD_GSK3_1	1	8	PF00069	0.588
MOD_GSK3_1	154	161	PF00069	0.430
MOD_GSK3_1	177	184	PF00069	0.442
MOD_GSK3_1	186	193	PF00069	0.377
MOD_GSK3_1	24	31	PF00069	0.271
MOD_GSK3_1	269	276	PF00069	0.344
MOD_GSK3_1	293	300	PF00069	0.460
MOD_GSK3_1	348	355	PF00069	0.226
MOD_GSK3_1	356	363	PF00069	0.342
MOD_GSK3_1	370	377	PF00069	0.390
MOD_GSK3_1	399	406	PF00069	0.368
MOD_GSK3_1	407	414	PF00069	0.356
MOD_GSK3_1	445	452	PF00069	0.382
MOD_GSK3_1	487	494	PF00069	0.326
MOD_GSK3_1	496	503	PF00069	0.289
MOD_GSK3_1	601	608	PF00069	0.656
MOD_GSK3_1	622	629	PF00069	0.636
MOD_GSK3_1	689	696	PF00069	0.574
MOD_N-GLC_2	441	443	PF02516	0.314
MOD_NEK2_1	17	22	PF00069	0.321
MOD_NEK2_1	186	191	PF00069	0.371
MOD_NEK2_1	220	225	PF00069	0.435
MOD_NEK2_1	273	278	PF00069	0.324
MOD_NEK2_1	28	33	PF00069	0.225
MOD_NEK2_1	297	302	PF00069	0.412
MOD_NEK2_1	329	334	PF00069	0.414
MOD_NEK2_1	387	392	PF00069	0.307
MOD_NEK2_1	407	412	PF00069	0.150
MOD_NEK2_1	449	454	PF00069	0.284
MOD_NEK2_1	475	480	PF00069	0.315
MOD_NEK2_1	488	493	PF00069	0.356
MOD_NEK2_1	591	596	PF00069	0.520
MOD_NEK2_1	654	659	PF00069	0.355
MOD_NEK2_2	356	361	PF00069	0.328
MOD_NEK2_2	585	590	PF00069	0.404
MOD_PIKK_1	166	172	PF00454	0.478
MOD_PIKK_1	354	360	PF00454	0.314
MOD_PIKK_1	482	488	PF00454	0.451
MOD_PK_1	175	181	PF00069	0.414
MOD_PK_1	661	667	PF00069	0.335
MOD_PKA_2	166	172	PF00069	0.505
MOD_PKA_2	259	265	PF00069	0.293
MOD_PKA_2	360	366	PF00069	0.233
MOD_PKA_2	392	398	PF00069	0.345
MOD_PKA_2	455	461	PF00069	0.384
MOD_PKB_1	704	712	PF00069	0.420
MOD_Plk_1	200	206	PF00069	0.382
MOD_Plk_1	449	455	PF00069	0.374
MOD_Plk_4	115	121	PF00069	0.471
MOD_Plk_4	154	160	PF00069	0.502
MOD_Plk_4	190	196	PF00069	0.490
MOD_Plk_4	200	206	PF00069	0.303
MOD_Plk_4	222	228	PF00069	0.447
MOD_Plk_4	234	240	PF00069	0.401
MOD_Plk_4	241	247	PF00069	0.361
MOD_Plk_4	29	35	PF00069	0.289
MOD_Plk_4	293	299	PF00069	0.376
MOD_Plk_4	348	354	PF00069	0.400
MOD_Plk_4	361	367	PF00069	0.420
MOD_Plk_4	374	380	PF00069	0.354
MOD_Plk_4	387	393	PF00069	0.222
MOD_Plk_4	403	409	PF00069	0.200
MOD_Plk_4	426	432	PF00069	0.304
MOD_Plk_4	488	494	PF00069	0.344
MOD_Plk_4	543	549	PF00069	0.424
MOD_Plk_4	568	574	PF00069	0.421
MOD_Plk_4	578	584	PF00069	0.355
MOD_Plk_4	585	591	PF00069	0.239
MOD_Plk_4	594	600	PF00069	0.396
MOD_Plk_4	637	643	PF00069	0.437
MOD_Plk_4	646	652	PF00069	0.365
MOD_Plk_4	661	667	PF00069	0.333
MOD_ProDKin_1	112	118	PF00069	0.461
MOD_ProDKin_1	308	314	PF00069	0.404
MOD_ProDKin_1	352	358	PF00069	0.332
MOD_ProDKin_1	496	502	PF00069	0.314
MOD_ProDKin_1	528	534	PF00069	0.438
MOD_ProDKin_1	592	598	PF00069	0.414
MOD_ProDKin_1	624	630	PF00069	0.620
MOD_ProDKin_1	689	695	PF00069	0.530
TRG_DiLeu_BaEn_1	234	239	PF01217	0.292
TRG_DiLeu_BaEn_1	403	408	PF01217	0.293
TRG_DiLeu_BaEn_1	685	690	PF01217	0.499
TRG_DiLeu_BaEn_2	439	445	PF01217	0.314
TRG_DiLeu_BaLyEn_6	33	38	PF01217	0.383
TRG_DiLeu_BaLyEn_6	430	435	PF01217	0.349
TRG_DiLeu_BaLyEn_6	696	701	PF01217	0.468
TRG_DiLeu_LyEn_5	685	690	PF01217	0.379
TRG_ENDOCYTIC_2	294	297	PF00928	0.404
TRG_ENDOCYTIC_2	420	423	PF00928	0.274
TRG_ENDOCYTIC_2	46	49	PF00928	0.452
TRG_ENDOCYTIC_2	472	475	PF00928	0.427
TRG_ENDOCYTIC_2	554	557	PF00928	0.294
TRG_ENDOCYTIC_2	590	593	PF00928	0.452
TRG_ER_diArg_1	686	688	PF00400	0.361
TRG_ER_diArg_1	697	700	PF00400	0.441
TRG_ER_diArg_1	703	706	PF00400	0.491
TRG_ER_diLys_1	725	729	PF00400	0.445
TRG_NES_CRM1_1	222	234	PF08389	0.413
TRG_Pf-PMV_PEXEL_1	271	275	PF00026	0.284
TRG_Pf-PMV_PEXEL_1	327	331	PF00026	0.193

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHL6	Leptomonas seymouri	59%	100%
A0A1X0NQN5	Trypanosomatidae	33%	100%
A0A3Q8IDZ2	Leishmania donovani	79%	100%
A0A3S5IQL5	Trypanosoma rangeli	35%	100%
A4I3X2	Leishmania infantum	79%	100%
D0A939	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9B062	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
Q4Q823	Leishmania major	79%	100%
V5AWN8	Trypanosoma cruzi	34%	100%