Adenine DNA glycosylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

A/G-specific adenine glycosylase, putative

Species:

Leishmania braziliensis

UniProt:

A4HGR8_LEIBR

TriTrypDb:

LbrM.28.2340 , LBRM2903_280029800

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HGR8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HGR8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006284	base-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006298	mismatch repair	6	1

Molecular functions
Term	Name	Level	Count
GO:0000700	mismatch base pair DNA N-glycosylase activity	5	12
GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity	6	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0016787	hydrolase activity	2	12
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	12
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	12
GO:0019104	DNA N-glycosylase activity	4	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0000702	oxidized base lesion DNA N-glycosylase activity	5	1
GO:0003676	nucleic acid binding	3	4
GO:0003677	DNA binding	4	4
GO:0003684	damaged DNA binding	5	1
GO:0003690	double-stranded DNA binding	5	1
GO:0008534	oxidized purine nucleobase lesion DNA N-glycosylase activity	6	1
GO:0030983	mismatched DNA binding	6	1
GO:0032356	oxidized DNA binding	6	1
GO:0032357	oxidized purine DNA binding	7	1
GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity	7	1
GO:0035485	adenine/guanine mispair binding	7	1
GO:0097159	organic cyclic compound binding	2	4
GO:1901363	heterocyclic compound binding	2	4
GO:0004518	nuclease activity	4	2
GO:0004519	endonuclease activity	5	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	124	126	PF00675	0.204
CLV_NRD_NRD_1	129	131	PF00675	0.204
CLV_NRD_NRD_1	314	316	PF00675	0.273
CLV_NRD_NRD_1	320	322	PF00675	0.273
CLV_NRD_NRD_1	485	487	PF00675	0.638
CLV_PCSK_KEX2_1	124	126	PF00082	0.204
CLV_PCSK_KEX2_1	293	295	PF00082	0.504
CLV_PCSK_KEX2_1	314	316	PF00082	0.274
CLV_PCSK_KEX2_1	320	322	PF00082	0.274
CLV_PCSK_KEX2_1	404	406	PF00082	0.551
CLV_PCSK_KEX2_1	458	460	PF00082	0.514
CLV_PCSK_KEX2_1	485	487	PF00082	0.564
CLV_PCSK_PC1ET2_1	293	295	PF00082	0.504
CLV_PCSK_PC1ET2_1	404	406	PF00082	0.434
CLV_PCSK_PC1ET2_1	458	460	PF00082	0.514
CLV_PCSK_SKI1_1	210	214	PF00082	0.299
CLV_PCSK_SKI1_1	287	291	PF00082	0.535
CLV_PCSK_SKI1_1	376	380	PF00082	0.282
CLV_PCSK_SKI1_1	427	431	PF00082	0.422
CLV_PCSK_SKI1_1	443	447	PF00082	0.261
DEG_APCC_DBOX_1	442	450	PF00400	0.362
DEG_Nend_UBRbox_1	1	4	PF02207	0.344
DEG_SCF_FBW7_1	6	11	PF00400	0.405
DOC_ANK_TNKS_1	319	326	PF00023	0.161
DOC_CKS1_1	5	10	PF01111	0.477
DOC_MAPK_gen_1	293	301	PF00069	0.351
DOC_MAPK_gen_1	363	371	PF00069	0.307
DOC_MAPK_gen_1	461	470	PF00069	0.573
DOC_MAPK_MEF2A_6	250	258	PF00069	0.250
DOC_PP1_RVXF_1	22	29	PF00149	0.210
DOC_PP2B_LxvP_1	159	162	PF13499	0.508
DOC_SPAK_OSR1_1	315	319	PF12202	0.273
DOC_USP7_MATH_1	166	170	PF00917	0.418
DOC_USP7_MATH_1	171	175	PF00917	0.391
DOC_USP7_MATH_1	203	207	PF00917	0.446
DOC_USP7_MATH_1	224	228	PF00917	0.513
DOC_USP7_MATH_1	292	296	PF00917	0.434
DOC_USP7_MATH_1	334	338	PF00917	0.267
DOC_USP7_MATH_1	44	48	PF00917	0.555
DOC_USP7_MATH_1	8	12	PF00917	0.410
DOC_WW_Pin1_4	145	150	PF00397	0.438
DOC_WW_Pin1_4	220	225	PF00397	0.484
DOC_WW_Pin1_4	4	9	PF00397	0.430
DOC_WW_Pin1_4	450	455	PF00397	0.409
DOC_WW_Pin1_4	475	480	PF00397	0.591
DOC_WW_Pin1_4	62	67	PF00397	0.392
LIG_14-3-3_CanoR_1	376	386	PF00244	0.268
LIG_14-3-3_CanoR_1	40	44	PF00244	0.465
LIG_14-3-3_CanoR_1	437	441	PF00244	0.304
LIG_14-3-3_CanoR_1	60	66	PF00244	0.504
LIG_Actin_WH2_2	424	439	PF00022	0.377
LIG_BRCT_BRCA1_1	173	177	PF00533	0.404
LIG_BRCT_BRCA1_1	50	54	PF00533	0.457
LIG_FHA_1	224	230	PF00498	0.498
LIG_FHA_1	465	471	PF00498	0.451
LIG_FHA_2	107	113	PF00498	0.520
LIG_FHA_2	357	363	PF00498	0.446
LIG_FHA_2	81	87	PF00498	0.432
LIG_IRF3_LxIS_1	254	259	PF10401	0.234
LIG_LIR_Apic_2	4	8	PF02991	0.473
LIG_LIR_Gen_1	415	426	PF02991	0.366
LIG_LIR_Nem_3	16	21	PF02991	0.357
LIG_LIR_Nem_3	332	336	PF02991	0.278
LIG_LIR_Nem_3	415	421	PF02991	0.384
LIG_LIR_Nem_3	65	71	PF02991	0.475
LIG_LRP6_Inhibitor_1	184	190	PF00058	0.308
LIG_Pex14_1	95	99	PF04695	0.404
LIG_RPA_C_Fungi	309	321	PF08784	0.281
LIG_SH2_CRK	18	22	PF00017	0.339
LIG_SH2_CRK	418	422	PF00017	0.376
LIG_SH2_NCK_1	167	171	PF00017	0.254
LIG_SH2_STAP1	167	171	PF00017	0.254
LIG_SH2_STAT5	135	138	PF00017	0.241
LIG_SH2_STAT5	167	170	PF00017	0.239
LIG_SH2_STAT5	418	421	PF00017	0.556
LIG_SH2_STAT5	92	95	PF00017	0.209
LIG_SH3_3	146	152	PF00018	0.216
LIG_SH3_3	245	251	PF00018	0.306
LIG_SH3_3	316	322	PF00018	0.298
LIG_SH3_3	388	394	PF00018	0.363
LIG_SUMO_SIM_anti_2	385	390	PF11976	0.249
LIG_SUMO_SIM_par_1	353	359	PF11976	0.426
LIG_SUMO_SIM_par_1	466	472	PF11976	0.452
LIG_TRAF2_1	153	156	PF00917	0.384
LIG_TRAF2_1	20	23	PF00917	0.348
LIG_TRAF2_1	469	472	PF00917	0.594
MOD_CDC14_SPxK_1	478	481	PF00782	0.593
MOD_CDK_SPK_2	62	67	PF00069	0.347
MOD_CDK_SPxK_1	475	481	PF00069	0.593
MOD_CDK_SPxxK_3	475	482	PF00069	0.592
MOD_CK1_1	101	107	PF00069	0.252
MOD_CK1_1	11	17	PF00069	0.530
MOD_CK1_1	180	186	PF00069	0.161
MOD_CK1_1	223	229	PF00069	0.561
MOD_CK1_1	356	362	PF00069	0.426
MOD_CK1_1	380	386	PF00069	0.346
MOD_CK1_1	439	445	PF00069	0.263
MOD_CK1_1	62	68	PF00069	0.477
MOD_CK1_1	80	86	PF00069	0.138
MOD_CK2_1	106	112	PF00069	0.343
MOD_CK2_1	150	156	PF00069	0.301
MOD_CK2_1	17	23	PF00069	0.442
MOD_CK2_1	292	298	PF00069	0.345
MOD_CK2_1	356	362	PF00069	0.380
MOD_CK2_1	450	456	PF00069	0.535
MOD_CK2_1	481	487	PF00069	0.448
MOD_CK2_1	80	86	PF00069	0.341
MOD_GlcNHglycan	11	14	PF01048	0.302
MOD_GlcNHglycan	168	171	PF01048	0.325
MOD_GlcNHglycan	205	208	PF01048	0.394
MOD_GlcNHglycan	226	229	PF01048	0.499
MOD_GlcNHglycan	233	237	PF01048	0.365
MOD_GlcNHglycan	268	271	PF01048	0.366
MOD_GlcNHglycan	304	308	PF01048	0.400
MOD_GlcNHglycan	336	339	PF01048	0.323
MOD_GlcNHglycan	438	441	PF01048	0.283
MOD_GlcNHglycan	46	49	PF01048	0.689
MOD_GlcNHglycan	50	53	PF01048	0.634
MOD_GlcNHglycan	61	64	PF01048	0.449
MOD_GSK3_1	101	108	PF00069	0.245
MOD_GSK3_1	220	227	PF00069	0.402
MOD_GSK3_1	332	339	PF00069	0.246
MOD_GSK3_1	4	11	PF00069	0.529
MOD_GSK3_1	44	51	PF00069	0.525
MOD_GSK3_1	460	467	PF00069	0.532
MOD_GSK3_1	471	478	PF00069	0.623
MOD_GSK3_1	58	65	PF00069	0.614
MOD_GSK3_1	73	80	PF00069	0.175
MOD_NEK2_1	177	182	PF00069	0.336
MOD_NEK2_1	244	249	PF00069	0.479
MOD_NEK2_1	256	261	PF00069	0.311
MOD_NEK2_1	371	376	PF00069	0.384
MOD_NEK2_1	436	441	PF00069	0.478
MOD_NEK2_1	9	14	PF00069	0.346
MOD_NEK2_2	292	297	PF00069	0.279
MOD_PIKK_1	151	157	PF00454	0.337
MOD_PIKK_1	77	83	PF00454	0.239
MOD_PK_1	98	104	PF00069	0.397
MOD_PKA_1	481	487	PF00069	0.448
MOD_PKA_2	364	370	PF00069	0.303
MOD_PKA_2	39	45	PF00069	0.460
MOD_PKA_2	436	442	PF00069	0.291
MOD_PKA_2	460	466	PF00069	0.585
MOD_PKA_2	59	65	PF00069	0.529
MOD_Plk_1	210	216	PF00069	0.249
MOD_Plk_4	112	118	PF00069	0.387
MOD_Plk_4	353	359	PF00069	0.303
MOD_Plk_4	84	90	PF00069	0.249
MOD_ProDKin_1	145	151	PF00069	0.287
MOD_ProDKin_1	220	226	PF00069	0.478
MOD_ProDKin_1	4	10	PF00069	0.433
MOD_ProDKin_1	450	456	PF00069	0.427
MOD_ProDKin_1	475	481	PF00069	0.593
MOD_ProDKin_1	62	68	PF00069	0.374
MOD_SUMO_rev_2	108	116	PF00179	0.387
MOD_SUMO_rev_2	198	206	PF00179	0.238
MOD_SUMO_rev_2	453	460	PF00179	0.541
TRG_ENDOCYTIC_2	18	21	PF00928	0.345
TRG_ENDOCYTIC_2	418	421	PF00928	0.381
TRG_ER_diArg_1	123	125	PF00400	0.239
TRG_ER_diArg_1	314	316	PF00400	0.274
TRG_ER_diArg_1	319	321	PF00400	0.274
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.320

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL90	Leptomonas seymouri	63%	96%
A0A0S4IRI2	Bodo saltans	40%	93%
A0A1X0NR87	Trypanosomatidae	46%	100%
A0A3S7X1P4	Leishmania donovani	82%	98%
A0A422N7D8	Trypanosoma rangeli	42%	100%
A4I3U3	Leishmania infantum	82%	98%
D0A913	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B035	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	98%
F4JRF4	Arabidopsis thaliana	33%	78%
O31584	Bacillus subtilis (strain 168)	30%	100%
P17802	Escherichia coli (strain K12)	29%	100%
P44320	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	27%	100%
Q05869	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	29%	100%
Q10159	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
Q4Q851	Leishmania major	81%	100%
Q8R5G2	Rattus norvegicus	32%	95%
Q99P21	Mus musculus	30%	95%
Q9UIF7	Homo sapiens	32%	90%
V5B3S1	Trypanosoma cruzi	42%	100%