TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania braziliensis

UniProt:

A4HGL5_LEIBR

TriTrypDb:

LbrM.28.1790 , LBRM2903_280024100

Length:

849

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HGL5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HGL5

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	2
GO:0016829	lyase activity	2	2
GO:0016835	carbon-oxygen lyase activity	3	2
GO:0016836	hydro-lyase activity	4	2
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	458	462	PF00656	0.285
CLV_NRD_NRD_1	153	155	PF00675	0.537
CLV_NRD_NRD_1	305	307	PF00675	0.361
CLV_NRD_NRD_1	308	310	PF00675	0.356
CLV_NRD_NRD_1	426	428	PF00675	0.271
CLV_NRD_NRD_1	430	432	PF00675	0.272
CLV_NRD_NRD_1	488	490	PF00675	0.265
CLV_NRD_NRD_1	537	539	PF00675	0.435
CLV_NRD_NRD_1	613	615	PF00675	0.265
CLV_NRD_NRD_1	731	733	PF00675	0.585
CLV_NRD_NRD_1	748	750	PF00675	0.398
CLV_NRD_NRD_1	799	801	PF00675	0.505
CLV_NRD_NRD_1	815	817	PF00675	0.531
CLV_PCSK_FUR_1	306	310	PF00082	0.406
CLV_PCSK_FUR_1	813	817	PF00082	0.538
CLV_PCSK_KEX2_1	153	155	PF00082	0.672
CLV_PCSK_KEX2_1	305	307	PF00082	0.408
CLV_PCSK_KEX2_1	308	310	PF00082	0.412
CLV_PCSK_KEX2_1	425	427	PF00082	0.265
CLV_PCSK_KEX2_1	430	432	PF00082	0.265
CLV_PCSK_KEX2_1	488	490	PF00082	0.265
CLV_PCSK_KEX2_1	537	539	PF00082	0.482
CLV_PCSK_KEX2_1	748	750	PF00082	0.484
CLV_PCSK_KEX2_1	757	759	PF00082	0.446
CLV_PCSK_KEX2_1	815	817	PF00082	0.627
CLV_PCSK_PC1ET2_1	757	759	PF00082	0.591
CLV_PCSK_PC7_1	426	432	PF00082	0.265
CLV_PCSK_PC7_1	744	750	PF00082	0.484
CLV_PCSK_SKI1_1	347	351	PF00082	0.281
CLV_PCSK_SKI1_1	407	411	PF00082	0.275
CLV_PCSK_SKI1_1	488	492	PF00082	0.291
CLV_PCSK_SKI1_1	633	637	PF00082	0.417
CLV_PCSK_SKI1_1	70	74	PF00082	0.541
CLV_PCSK_SKI1_1	757	761	PF00082	0.510
CLV_PCSK_SKI1_1	767	771	PF00082	0.400
CLV_PCSK_SKI1_1	815	819	PF00082	0.598
CLV_Separin_Metazoa	607	611	PF03568	0.265
DEG_APCC_DBOX_1	288	296	PF00400	0.279
DOC_CKS1_1	4	9	PF01111	0.536
DOC_CYCLIN_RxL_1	610	622	PF00134	0.358
DOC_CYCLIN_RxL_1	755	763	PF00134	0.542
DOC_MAPK_gen_1	305	316	PF00069	0.291
DOC_MAPK_gen_1	38	47	PF00069	0.548
DOC_MAPK_gen_1	425	437	PF00069	0.348
DOC_MAPK_MEF2A_6	308	316	PF00069	0.187
DOC_PP1_RVXF_1	588	594	PF00149	0.187
DOC_PP1_RVXF_1	755	762	PF00149	0.508
DOC_PP2B_LxvP_1	664	667	PF13499	0.421
DOC_PP2B_LxvP_1	722	725	PF13499	0.533
DOC_PP4_FxxP_1	4	7	PF00568	0.546
DOC_PP4_FxxP_1	605	608	PF00568	0.265
DOC_PP4_FxxP_1	74	77	PF00568	0.567
DOC_PP4_FxxP_1	761	764	PF00568	0.365
DOC_USP7_MATH_1	417	421	PF00917	0.187
DOC_USP7_MATH_1	595	599	PF00917	0.417
DOC_USP7_MATH_1	708	712	PF00917	0.703
DOC_USP7_MATH_1	78	82	PF00917	0.708
DOC_USP7_MATH_1	786	790	PF00917	0.586
DOC_USP7_MATH_1	819	823	PF00917	0.508
DOC_WW_Pin1_4	103	108	PF00397	0.724
DOC_WW_Pin1_4	293	298	PF00397	0.265
DOC_WW_Pin1_4	3	8	PF00397	0.500
DOC_WW_Pin1_4	760	765	PF00397	0.362
DOC_WW_Pin1_4	804	809	PF00397	0.613
LIG_14-3-3_CanoR_1	272	277	PF00244	0.419
LIG_14-3-3_CanoR_1	370	374	PF00244	0.265
LIG_14-3-3_CanoR_1	488	495	PF00244	0.261
LIG_Actin_WH2_2	461	479	PF00022	0.410
LIG_AP2alpha_2	729	731	PF02296	0.623
LIG_BRCT_BRCA1_1	68	72	PF00533	0.619
LIG_BRCT_BRCA1_1	843	847	PF00533	0.573
LIG_CaM_IQ_9	121	136	PF13499	0.519
LIG_CtBP_PxDLS_1	616	620	PF00389	0.279
LIG_deltaCOP1_diTrp_1	539	544	PF00928	0.410
LIG_EH1_1	22	30	PF00400	0.347
LIG_eIF4E_1	321	327	PF01652	0.298
LIG_FHA_1	194	200	PF00498	0.734
LIG_FHA_1	213	219	PF00498	0.509
LIG_FHA_1	24	30	PF00498	0.419
LIG_FHA_1	264	270	PF00498	0.336
LIG_FHA_1	503	509	PF00498	0.279
LIG_FHA_1	525	531	PF00498	0.197
LIG_FHA_1	829	835	PF00498	0.702
LIG_FHA_2	37	43	PF00498	0.580
LIG_FHA_2	398	404	PF00498	0.284
LIG_FHA_2	438	444	PF00498	0.228
LIG_FHA_2	456	462	PF00498	0.176
LIG_FHA_2	530	536	PF00498	0.323
LIG_FHA_2	541	547	PF00498	0.330
LIG_FHA_2	555	561	PF00498	0.322
LIG_FHA_2	682	688	PF00498	0.481
LIG_HCF-1_HBM_1	678	681	PF13415	0.343
LIG_LIR_Apic_2	604	608	PF02991	0.265
LIG_LIR_Apic_2	71	77	PF02991	0.525
LIG_LIR_Gen_1	379	390	PF02991	0.265
LIG_LIR_Gen_1	497	508	PF02991	0.225
LIG_LIR_Gen_1	539	548	PF02991	0.410
LIG_LIR_Nem_3	120	126	PF02991	0.539
LIG_LIR_Nem_3	359	365	PF02991	0.301
LIG_LIR_Nem_3	379	385	PF02991	0.145
LIG_LIR_Nem_3	478	483	PF02991	0.319
LIG_LIR_Nem_3	497	503	PF02991	0.161
LIG_LIR_Nem_3	539	544	PF02991	0.410
LIG_LIR_Nem_3	567	571	PF02991	0.291
LIG_LIR_Nem_3	668	674	PF02991	0.592
LIG_LIR_Nem_3	687	692	PF02991	0.296
LIG_LIR_Nem_3	836	840	PF02991	0.581
LIG_LIR_Nem_3	9	15	PF02991	0.494
LIG_NRBOX	405	411	PF00104	0.265
LIG_PCNA_PIPBox_1	575	584	PF02747	0.298
LIG_PCNA_yPIPBox_3	575	588	PF02747	0.420
LIG_PCNA_yPIPBox_3	638	650	PF02747	0.265
LIG_Pex14_1	540	544	PF04695	0.410
LIG_Pex14_2	12	16	PF04695	0.460
LIG_Pex14_2	332	336	PF04695	0.265
LIG_Pex14_2	392	396	PF04695	0.279
LIG_SH2_CRK	641	645	PF00017	0.265
LIG_SH2_CRK	794	798	PF00017	0.600
LIG_SH2_GRB2like	641	644	PF00017	0.265
LIG_SH2_NCK_1	623	627	PF00017	0.333
LIG_SH2_NCK_1	641	645	PF00017	0.211
LIG_SH2_SRC	623	626	PF00017	0.421
LIG_SH2_SRC	641	644	PF00017	0.202
LIG_SH2_SRC	692	695	PF00017	0.574
LIG_SH2_STAP1	213	217	PF00017	0.471
LIG_SH2_STAP1	623	627	PF00017	0.361
LIG_SH2_STAP1	641	645	PF00017	0.211
LIG_SH2_STAP1	794	798	PF00017	0.600
LIG_SH2_STAT3	366	369	PF00017	0.289
LIG_SH2_STAT3	556	559	PF00017	0.187
LIG_SH2_STAT3	581	584	PF00017	0.279
LIG_SH2_STAT5	244	247	PF00017	0.265
LIG_SH2_STAT5	282	285	PF00017	0.279
LIG_SH2_STAT5	399	402	PF00017	0.279
LIG_SH2_STAT5	480	483	PF00017	0.346
LIG_SH2_STAT5	556	559	PF00017	0.358
LIG_SH2_STAT5	582	585	PF00017	0.199
LIG_SH2_STAT5	681	684	PF00017	0.388
LIG_SH3_1	112	118	PF00018	0.511
LIG_SH3_1	158	164	PF00018	0.528
LIG_SH3_3	112	118	PF00018	0.664
LIG_SH3_3	158	164	PF00018	0.528
LIG_SH3_3	322	328	PF00018	0.266
LIG_SH3_3	340	346	PF00018	0.279
LIG_SH3_3	558	564	PF00018	0.311
LIG_SH3_3	605	611	PF00018	0.265
LIG_SH3_3	74	80	PF00018	0.540
LIG_SH3_3	779	785	PF00018	0.473
LIG_SUMO_SIM_anti_2	658	665	PF11976	0.436
LIG_SUMO_SIM_par_1	26	32	PF11976	0.521
LIG_SUMO_SIM_par_1	615	620	PF11976	0.327
LIG_WRC_WIRS_1	495	500	PF05994	0.410
LIG_WRC_WIRS_1	541	546	PF05994	0.399
LIG_WRC_WIRS_1	834	839	PF05994	0.653
LIG_WW_2	77	80	PF00397	0.659
LIG_WW_3	607	611	PF00397	0.265
MOD_CDK_SPK_2	804	809	PF00069	0.618
MOD_CDK_SPxxK_3	760	767	PF00069	0.358
MOD_CK1_1	233	239	PF00069	0.483
MOD_CK1_1	317	323	PF00069	0.276
MOD_CK1_1	36	42	PF00069	0.778
MOD_CK1_1	669	675	PF00069	0.388
MOD_CK1_1	822	828	PF00069	0.671
MOD_CK1_1	830	836	PF00069	0.596
MOD_CK1_1	841	847	PF00069	0.352
MOD_CK2_1	36	42	PF00069	0.619
MOD_CK2_1	402	408	PF00069	0.288
MOD_CK2_1	417	423	PF00069	0.323
MOD_CK2_1	463	469	PF00069	0.291
MOD_CK2_1	529	535	PF00069	0.392
MOD_CK2_1	706	712	PF00069	0.566
MOD_CK2_1	79	85	PF00069	0.556
MOD_GlcNHglycan	164	167	PF01048	0.553
MOD_GlcNHglycan	170	173	PF01048	0.557
MOD_GlcNHglycan	221	224	PF01048	0.642
MOD_GlcNHglycan	235	238	PF01048	0.598
MOD_GlcNHglycan	453	456	PF01048	0.339
MOD_GlcNHglycan	599	602	PF01048	0.358
MOD_GlcNHglycan	671	674	PF01048	0.624
MOD_GlcNHglycan	708	711	PF01048	0.567
MOD_GlcNHglycan	788	791	PF01048	0.560
MOD_GlcNHglycan	827	830	PF01048	0.627
MOD_GSK3_1	180	187	PF00069	0.782
MOD_GSK3_1	32	39	PF00069	0.578
MOD_GSK3_1	451	458	PF00069	0.350
MOD_GSK3_1	484	491	PF00069	0.298
MOD_GSK3_1	524	531	PF00069	0.392
MOD_GSK3_1	655	662	PF00069	0.416
MOD_GSK3_1	706	713	PF00069	0.727
MOD_GSK3_1	815	822	PF00069	0.724
MOD_GSK3_1	824	831	PF00069	0.642
MOD_GSK3_1	841	848	PF00069	0.346
MOD_LATS_1	270	276	PF00433	0.410
MOD_N-GLC_1	397	402	PF02516	0.358
MOD_N-GLC_1	786	791	PF02516	0.580
MOD_NEK2_1	245	250	PF00069	0.438
MOD_NEK2_1	72	77	PF00069	0.589
MOD_NEK2_2	33	38	PF00069	0.469
MOD_NEK2_2	628	633	PF00069	0.327
MOD_PIKK_1	184	190	PF00454	0.521
MOD_PIKK_1	417	423	PF00454	0.327
MOD_PIKK_1	482	488	PF00454	0.225
MOD_PIKK_1	6	12	PF00454	0.507
MOD_PIKK_1	736	742	PF00454	0.658
MOD_PK_1	272	278	PF00069	0.410
MOD_PK_1	291	297	PF00069	0.410
MOD_PK_1	341	347	PF00069	0.327
MOD_PKA_1	488	494	PF00069	0.265
MOD_PKA_1	815	821	PF00069	0.669
MOD_PKA_2	369	375	PF00069	0.265
MOD_PKA_2	488	494	PF00069	0.265
MOD_PKA_2	546	552	PF00069	0.442
MOD_PKA_2	554	560	PF00069	0.145
MOD_PKA_2	743	749	PF00069	0.608
MOD_PKA_2	815	821	PF00069	0.611
MOD_PKB_1	289	297	PF00069	0.187
MOD_PKB_1	813	821	PF00069	0.614
MOD_Plk_1	119	125	PF00069	0.633
MOD_Plk_1	213	219	PF00069	0.622
MOD_Plk_1	317	323	PF00069	0.279
MOD_Plk_1	341	347	PF00069	0.381
MOD_Plk_1	402	408	PF00069	0.298
MOD_Plk_1	617	623	PF00069	0.395
MOD_Plk_4	119	125	PF00069	0.578
MOD_Plk_4	213	219	PF00069	0.474
MOD_Plk_4	494	500	PF00069	0.274
MOD_Plk_4	659	665	PF00069	0.479
MOD_Plk_4	666	672	PF00069	0.499
MOD_Plk_4	79	85	PF00069	0.571
MOD_ProDKin_1	103	109	PF00069	0.724
MOD_ProDKin_1	293	299	PF00069	0.265
MOD_ProDKin_1	3	9	PF00069	0.498
MOD_ProDKin_1	760	766	PF00069	0.356
MOD_ProDKin_1	804	810	PF00069	0.617
MOD_SUMO_rev_2	69	75	PF00179	0.740
MOD_SUMO_rev_2	706	715	PF00179	0.632
MOD_SUMO_rev_2	750	759	PF00179	0.557
TRG_DiLeu_BaLyEn_6	755	760	PF01217	0.509
TRG_ENDOCYTIC_2	480	483	PF00928	0.358
TRG_ENDOCYTIC_2	582	585	PF00928	0.298
TRG_ENDOCYTIC_2	641	644	PF00928	0.278
TRG_ENDOCYTIC_2	794	797	PF00928	0.607
TRG_ER_diArg_1	128	131	PF00400	0.685
TRG_ER_diArg_1	152	154	PF00400	0.616
TRG_ER_diArg_1	289	292	PF00400	0.265
TRG_ER_diArg_1	305	307	PF00400	0.265
TRG_ER_diArg_1	37	40	PF00400	0.480
TRG_ER_diArg_1	424	427	PF00400	0.265
TRG_ER_diArg_1	429	431	PF00400	0.265
TRG_ER_diArg_1	488	490	PF00400	0.265
TRG_ER_diArg_1	553	556	PF00400	0.327
TRG_ER_diArg_1	812	815	PF00400	0.563
TRG_NES_CRM1_1	281	293	PF08389	0.323
TRG_Pf-PMV_PEXEL_1	404	408	PF00026	0.275
TRG_Pf-PMV_PEXEL_1	430	434	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	488	493	PF00026	0.279
TRG_Pf-PMV_PEXEL_1	515	520	PF00026	0.460
TRG_Pf-PMV_PEXEL_1	654	659	PF00026	0.470
TRG_Pf-PMV_PEXEL_1	66	71	PF00026	0.500
TRG_Pf-PMV_PEXEL_1	758	762	PF00026	0.511
TRG_Pf-PMV_PEXEL_1	767	771	PF00026	0.457

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P820	Leptomonas seymouri	66%	86%
A0A0S4K0L8	Bodo saltans	45%	84%
A0A1X0NQX7	Trypanosomatidae	56%	92%
A0A3Q8II50	Leishmania donovani	84%	100%
A0A3S5ISD4	Trypanosoma rangeli	53%	97%
A4I3P1	Leishmania infantum	89%	100%
D0A8C3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	92%
E9AZY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
Q4Q8A3	Leishmania major	84%	100%
V5BFB8	Trypanosoma cruzi	59%	92%