DNA polymerase kappa

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase kappa, putative

Species:

Leishmania braziliensis

UniProt:

A4HGJ3_LEIBR

TriTrypDb:

LbrM.28.1570 , LBRM2903_280021700

Length:

754

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A4HGJ3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HGJ3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006260	DNA replication	5	2
GO:0006281	DNA repair	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0000731	DNA synthesis involved in DNA repair	6	2
GO:0006301	postreplication repair	6	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0019985	translesion synthesis	7	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0042276	error-prone translesion synthesis	8	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0071897	DNA biosynthetic process	5	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003684	damaged DNA binding	5	12
GO:0003824	catalytic activity	1	12
GO:0003887	DNA-directed DNA polymerase activity	5	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034061	DNA polymerase activity	4	12
GO:0043167	ion binding	2	2
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0097159	organic cyclic compound binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	674	678	PF00656	0.670
CLV_NRD_NRD_1	178	180	PF00675	0.382
CLV_NRD_NRD_1	183	185	PF00675	0.387
CLV_NRD_NRD_1	364	366	PF00675	0.361
CLV_NRD_NRD_1	418	420	PF00675	0.224
CLV_NRD_NRD_1	454	456	PF00675	0.366
CLV_NRD_NRD_1	503	505	PF00675	0.264
CLV_NRD_NRD_1	576	578	PF00675	0.223
CLV_NRD_NRD_1	605	607	PF00675	0.223
CLV_NRD_NRD_1	662	664	PF00675	0.567
CLV_PCSK_FUR_1	183	187	PF00082	0.315
CLV_PCSK_KEX2_1	183	185	PF00082	0.403
CLV_PCSK_KEX2_1	363	365	PF00082	0.346
CLV_PCSK_KEX2_1	418	420	PF00082	0.168
CLV_PCSK_KEX2_1	503	505	PF00082	0.292
CLV_PCSK_KEX2_1	576	578	PF00082	0.223
CLV_PCSK_KEX2_1	604	606	PF00082	0.223
CLV_PCSK_KEX2_1	664	666	PF00082	0.545
CLV_PCSK_PC1ET2_1	185	187	PF00082	0.324
CLV_PCSK_PC1ET2_1	664	666	PF00082	0.658
CLV_PCSK_PC7_1	179	185	PF00082	0.287
CLV_PCSK_SKI1_1	124	128	PF00082	0.508
CLV_PCSK_SKI1_1	141	145	PF00082	0.254
CLV_PCSK_SKI1_1	576	580	PF00082	0.223
CLV_Separin_Metazoa	573	577	PF03568	0.423
DEG_APCC_DBOX_1	445	453	PF00400	0.319
DEG_APCC_DBOX_1	518	526	PF00400	0.435
DEG_APCC_DBOX_1	575	583	PF00400	0.423
DEG_SPOP_SBC_1	129	133	PF00917	0.476
DEG_SPOP_SBC_1	483	487	PF00917	0.518
DOC_ANK_TNKS_1	418	425	PF00023	0.368
DOC_CKS1_1	68	73	PF01111	0.510
DOC_CYCLIN_RxL_1	557	571	PF00134	0.368
DOC_MAPK_gen_1	183	193	PF00069	0.375
DOC_MAPK_gen_1	515	522	PF00069	0.368
DOC_MAPK_gen_1	576	584	PF00069	0.397
DOC_MAPK_gen_1	592	600	PF00069	0.360
DOC_MAPK_HePTP_8	574	586	PF00069	0.419
DOC_MAPK_MEF2A_6	227	236	PF00069	0.356
DOC_MAPK_MEF2A_6	289	296	PF00069	0.437
DOC_MAPK_MEF2A_6	577	586	PF00069	0.419
DOC_MAPK_MEF2A_6	592	600	PF00069	0.419
DOC_MAPK_RevD_3	234	249	PF00069	0.232
DOC_MIT_MIM_1	593	605	PF04212	0.407
DOC_PP1_RVXF_1	139	146	PF00149	0.425
DOC_PP2B_LxvP_1	582	585	PF13499	0.442
DOC_SPAK_OSR1_1	351	355	PF12202	0.423
DOC_USP7_MATH_1	10	14	PF00917	0.721
DOC_USP7_MATH_1	113	117	PF00917	0.738
DOC_USP7_MATH_1	119	123	PF00917	0.593
DOC_USP7_MATH_1	129	133	PF00917	0.495
DOC_USP7_MATH_1	607	611	PF00917	0.507
DOC_USP7_MATH_1	63	67	PF00917	0.509
DOC_USP7_MATH_1	638	642	PF00917	0.567
DOC_USP7_MATH_1	710	714	PF00917	0.564
DOC_USP7_UBL2_3	559	563	PF12436	0.423
DOC_USP7_UBL2_3	730	734	PF12436	0.506
DOC_WW_Pin1_4	109	114	PF00397	0.478
DOC_WW_Pin1_4	11	16	PF00397	0.633
DOC_WW_Pin1_4	33	38	PF00397	0.572
DOC_WW_Pin1_4	378	383	PF00397	0.407
DOC_WW_Pin1_4	43	48	PF00397	0.570
DOC_WW_Pin1_4	59	64	PF00397	0.546
DOC_WW_Pin1_4	648	653	PF00397	0.505
DOC_WW_Pin1_4	67	72	PF00397	0.623
DOC_WW_Pin1_4	715	720	PF00397	0.618
LIG_14-3-3_CanoR_1	25	29	PF00244	0.587
LIG_14-3-3_CanoR_1	455	459	PF00244	0.339
LIG_14-3-3_CanoR_1	503	508	PF00244	0.230
LIG_14-3-3_CanoR_1	531	538	PF00244	0.441
LIG_14-3-3_CanoR_1	59	63	PF00244	0.494
LIG_14-3-3_CanoR_1	639	648	PF00244	0.518
LIG_Actin_WH2_2	518	533	PF00022	0.485
LIG_APCC_ABBA_1	232	237	PF00400	0.236
LIG_APCC_ABBA_1	565	570	PF00400	0.442
LIG_BIR_II_1	1	5	PF00653	0.599
LIG_BRCT_BRCA1_1	122	126	PF00533	0.459
LIG_BRCT_BRCA1_1	307	311	PF00533	0.442
LIG_BRCT_BRCA1_1	633	637	PF00533	0.510
LIG_FHA_1	129	135	PF00498	0.442
LIG_FHA_1	158	164	PF00498	0.374
LIG_FHA_1	267	273	PF00498	0.408
LIG_FHA_1	379	385	PF00498	0.437
LIG_FHA_1	485	491	PF00498	0.485
LIG_FHA_2	345	351	PF00498	0.371
LIG_FHA_2	436	442	PF00498	0.325
LIG_FHA_2	700	706	PF00498	0.533
LIG_LIR_Apic_2	389	394	PF02991	0.507
LIG_LIR_Gen_1	228	236	PF02991	0.351
LIG_LIR_Gen_1	239	247	PF02991	0.418
LIG_LIR_Gen_1	457	466	PF02991	0.356
LIG_LIR_Gen_1	569	579	PF02991	0.540
LIG_LIR_Gen_1	588	598	PF02991	0.428
LIG_LIR_Nem_3	207	212	PF02991	0.388
LIG_LIR_Nem_3	220	226	PF02991	0.377
LIG_LIR_Nem_3	228	232	PF02991	0.316
LIG_LIR_Nem_3	239	245	PF02991	0.424
LIG_LIR_Nem_3	308	314	PF02991	0.461
LIG_LIR_Nem_3	457	461	PF02991	0.291
LIG_LIR_Nem_3	510	516	PF02991	0.461
LIG_LIR_Nem_3	569	575	PF02991	0.540
LIG_LIR_Nem_3	588	593	PF02991	0.414
LIG_LIR_Nem_3	98	102	PF02991	0.500
LIG_MLH1_MIPbox_1	633	637	PF16413	0.510
LIG_NRBOX	578	584	PF00104	0.442
LIG_PCNA_PIPBox_1	331	340	PF02747	0.442
LIG_PCNA_PIPBox_1	630	639	PF02747	0.540
LIG_PCNA_yPIPBox_3	630	639	PF02747	0.540
LIG_PTB_Apo_2	247	254	PF02174	0.442
LIG_PTB_Phospho_1	247	253	PF10480	0.442
LIG_Rb_pABgroove_1	466	474	PF01858	0.402
LIG_REV1ctd_RIR_1	634	643	PF16727	0.569
LIG_SH2_CRK	391	395	PF00017	0.376
LIG_SH2_CRK	99	103	PF00017	0.492
LIG_SH2_GRB2like	128	131	PF00017	0.458
LIG_SH2_GRB2like	174	177	PF00017	0.323
LIG_SH2_NCK_1	242	246	PF00017	0.452
LIG_SH2_NCK_1	253	257	PF00017	0.440
LIG_SH2_NCK_1	318	322	PF00017	0.368
LIG_SH2_PTP2	233	236	PF00017	0.245
LIG_SH2_SRC	233	236	PF00017	0.295
LIG_SH2_SRC	253	256	PF00017	0.406
LIG_SH2_SRC	318	321	PF00017	0.368
LIG_SH2_STAP1	458	462	PF00017	0.293
LIG_SH2_STAP1	568	572	PF00017	0.381
LIG_SH2_STAT3	337	340	PF00017	0.371
LIG_SH2_STAT5	174	177	PF00017	0.388
LIG_SH2_STAT5	233	236	PF00017	0.278
LIG_SH2_STAT5	271	274	PF00017	0.473
LIG_SH2_STAT5	337	340	PF00017	0.437
LIG_SH2_STAT5	411	414	PF00017	0.538
LIG_SH2_STAT5	451	454	PF00017	0.310
LIG_SH2_STAT5	568	571	PF00017	0.430
LIG_SH3_2	38	43	PF14604	0.476
LIG_SH3_3	292	298	PF00018	0.442
LIG_SH3_3	35	41	PF00018	0.568
LIG_SH3_3	44	50	PF00018	0.563
LIG_SH3_3	65	71	PF00018	0.523
LIG_SH3_3	704	710	PF00018	0.643
LIG_SH3_4	393	400	PF00018	0.334
LIG_SUMO_SIM_anti_2	747	754	PF11976	0.521
LIG_SUMO_SIM_par_1	293	300	PF11976	0.442
LIG_SUMO_SIM_par_1	616	623	PF11976	0.472
LIG_SUMO_SIM_par_1	691	699	PF11976	0.584
LIG_SUMO_SIM_par_1	747	754	PF11976	0.634
LIG_UBA3_1	163	169	PF00899	0.369
LIG_UBA3_1	449	456	PF00899	0.388
LIG_UBA3_1	595	602	PF00899	0.174
LIG_UBA3_1	659	664	PF00899	0.482
LIG_WRC_WIRS_1	226	231	PF05994	0.279
LIG_WW_3	40	44	PF00397	0.469
MOD_CDK_SPxK_1	648	654	PF00069	0.505
MOD_CDK_SPxK_1	67	73	PF00069	0.541
MOD_CK1_1	105	111	PF00069	0.461
MOD_CK1_1	13	19	PF00069	0.541
MOD_CK1_1	168	174	PF00069	0.374
MOD_CK1_1	228	234	PF00069	0.329
MOD_CK1_1	27	33	PF00069	0.481
MOD_CK1_1	426	432	PF00069	0.290
MOD_CK1_1	482	488	PF00069	0.521
MOD_CK1_1	533	539	PF00069	0.252
MOD_CK1_1	696	702	PF00069	0.573
MOD_CK1_1	742	748	PF00069	0.531
MOD_CK1_1	88	94	PF00069	0.606
MOD_CK1_1	95	101	PF00069	0.565
MOD_CK2_1	344	350	PF00069	0.192
MOD_CK2_1	435	441	PF00069	0.308
MOD_CK2_1	619	625	PF00069	0.499
MOD_CK2_1	699	705	PF00069	0.549
MOD_GlcNHglycan	1	4	PF01048	0.590
MOD_GlcNHglycan	104	107	PF01048	0.483
MOD_GlcNHglycan	279	282	PF01048	0.485
MOD_GlcNHglycan	307	310	PF01048	0.284
MOD_GlcNHglycan	374	377	PF01048	0.369
MOD_GlcNHglycan	463	466	PF01048	0.234
MOD_GlcNHglycan	481	484	PF01048	0.702
MOD_GlcNHglycan	6	10	PF01048	0.621
MOD_GlcNHglycan	609	612	PF01048	0.534
MOD_GlcNHglycan	640	643	PF01048	0.614
MOD_GlcNHglycan	671	674	PF01048	0.501
MOD_GlcNHglycan	677	681	PF01048	0.526
MOD_GlcNHglycan	743	747	PF01048	0.700
MOD_GlcNHglycan	88	91	PF01048	0.582
MOD_GSK3_1	109	116	PF00069	0.547
MOD_GSK3_1	13	20	PF00069	0.524
MOD_GSK3_1	130	137	PF00069	0.647
MOD_GSK3_1	478	485	PF00069	0.575
MOD_GSK3_1	55	62	PF00069	0.602
MOD_GSK3_1	616	623	PF00069	0.498
MOD_GSK3_1	625	632	PF00069	0.531
MOD_GSK3_1	63	70	PF00069	0.569
MOD_GSK3_1	676	683	PF00069	0.506
MOD_GSK3_1	84	91	PF00069	0.687
MOD_GSK3_1	98	105	PF00069	0.630
MOD_LATS_1	557	563	PF00433	0.279
MOD_N-GLC_1	129	134	PF02516	0.493
MOD_N-GLC_1	675	680	PF02516	0.504
MOD_N-GLC_1	85	90	PF02516	0.570
MOD_N-GLC_2	201	203	PF02516	0.274
MOD_NEK2_1	145	150	PF00069	0.360
MOD_NEK2_1	192	197	PF00069	0.393
MOD_NEK2_1	265	270	PF00069	0.274
MOD_NEK2_1	343	348	PF00069	0.249
MOD_NEK2_1	372	377	PF00069	0.325
MOD_NEK2_1	461	466	PF00069	0.356
MOD_NEK2_1	542	547	PF00069	0.248
MOD_NEK2_1	85	90	PF00069	0.535
MOD_PIKK_1	542	548	PF00454	0.263
MOD_PIKK_1	626	632	PF00454	0.471
MOD_PIKK_1	696	702	PF00454	0.530
MOD_PK_1	503	509	PF00069	0.174
MOD_PKA_1	503	509	PF00069	0.174
MOD_PKA_2	24	30	PF00069	0.478
MOD_PKA_2	4	10	PF00069	0.592
MOD_PKA_2	426	432	PF00069	0.252
MOD_PKA_2	454	460	PF00069	0.339
MOD_PKA_2	503	509	PF00069	0.229
MOD_PKA_2	530	536	PF00069	0.252
MOD_PKA_2	58	64	PF00069	0.518
MOD_PKA_2	638	644	PF00069	0.470
MOD_PKA_2	666	672	PF00069	0.620
MOD_PKB_1	665	673	PF00069	0.485
MOD_Plk_1	129	135	PF00069	0.442
MOD_Plk_1	219	225	PF00069	0.259
MOD_Plk_1	509	515	PF00069	0.271
MOD_Plk_4	228	234	PF00069	0.297
MOD_Plk_4	24	30	PF00069	0.548
MOD_Plk_4	426	432	PF00069	0.279
MOD_Plk_4	533	539	PF00069	0.267
MOD_ProDKin_1	109	115	PF00069	0.478
MOD_ProDKin_1	11	17	PF00069	0.625
MOD_ProDKin_1	33	39	PF00069	0.574
MOD_ProDKin_1	378	384	PF00069	0.229
MOD_ProDKin_1	43	49	PF00069	0.567
MOD_ProDKin_1	59	65	PF00069	0.546
MOD_ProDKin_1	648	654	PF00069	0.505
MOD_ProDKin_1	67	73	PF00069	0.625
MOD_ProDKin_1	715	721	PF00069	0.615
MOD_SUMO_for_1	158	161	PF00179	0.285
MOD_SUMO_rev_2	172	181	PF00179	0.398
MOD_SUMO_rev_2	389	394	PF00179	0.266
TRG_DiLeu_BaLyEn_6	591	596	PF01217	0.266
TRG_ENDOCYTIC_2	226	229	PF00928	0.351
TRG_ENDOCYTIC_2	233	236	PF00928	0.274
TRG_ENDOCYTIC_2	242	245	PF00928	0.254
TRG_ENDOCYTIC_2	253	256	PF00928	0.151
TRG_ENDOCYTIC_2	458	461	PF00928	0.371
TRG_ENDOCYTIC_2	472	475	PF00928	0.627
TRG_ENDOCYTIC_2	572	575	PF00928	0.425
TRG_ENDOCYTIC_2	99	102	PF00928	0.498
TRG_ER_diArg_1	183	186	PF00400	0.372
TRG_ER_diArg_1	363	365	PF00400	0.261
TRG_ER_diArg_1	417	419	PF00400	0.174
TRG_ER_diArg_1	446	449	PF00400	0.315
TRG_ER_diArg_1	502	504	PF00400	0.340
TRG_ER_diArg_1	575	577	PF00400	0.252
TRG_ER_diArg_1	603	606	PF00400	0.252
TRG_ER_diArg_1	662	665	PF00400	0.556
TRG_NES_CRM1_1	521	532	PF08389	0.174
TRG_NES_CRM1_1	573	588	PF08389	0.269
TRG_NLS_MonoExtC_3	183	189	PF00514	0.288
TRG_NLS_MonoExtC_3	662	667	PF00514	0.562
TRG_NLS_MonoExtN_4	183	188	PF00514	0.287
TRG_Pf-PMV_PEXEL_1	210	215	PF00026	0.339
TRG_Pf-PMV_PEXEL_1	357	361	PF00026	0.252
TRG_Pf-PMV_PEXEL_1	577	581	PF00026	0.279

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7I6	Leptomonas seymouri	61%	100%
A0A0S4JDU0	Bodo saltans	38%	85%
A0A3Q8IEM0	Leishmania donovani	72%	100%
A0A3S7X1J7	Leishmania donovani	37%	100%
A4I3M0	Leishmania infantum	72%	100%
A4I3M1	Leishmania infantum	37%	100%
E9AZW1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	71%	100%
E9AZW2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
P34409	Caenorhabditis elegans	31%	100%
Q4Q8C4	Leishmania major	37%	100%
Q4Q8C5	Leishmania major	71%	99%