Putative endo/exonuclease Mre11

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

endo/exonuclease Mre11, putative

Species:

Leishmania braziliensis

UniProt:

A4HFW3_LEIBR

TriTrypDb:

LbrM.27.2030 , LBRM2903_270026800

Length:

863

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	6
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043231	intracellular membrane-bounded organelle	4	6
GO:0110165	cellular anatomical entity	1	7
GO:0030870	Mre11 complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0035861	site of double-strand break	3	1
GO:0090734	site of DNA damage	2	1
GO:0140513	nuclear protein-containing complex	2	2

Expansion

Sequence features

A4HFW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HFW3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006259	DNA metabolic process	4	8
GO:0006281	DNA repair	5	8
GO:0006302	double-strand break repair	6	8
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0006950	response to stress	2	8
GO:0006974	DNA damage response	4	8
GO:0008152	metabolic process	1	8
GO:0009987	cellular process	1	8
GO:0033554	cellular response to stress	3	8
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0043170	macromolecule metabolic process	3	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0044260	obsolete cellular macromolecule metabolic process	3	8
GO:0046483	heterocycle metabolic process	3	8
GO:0050896	response to stimulus	1	8
GO:0051716	cellular response to stimulus	2	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0000075	cell cycle checkpoint signaling	4	1
GO:0000077	DNA damage checkpoint signaling	5	1
GO:0000723	telomere maintenance	5	1
GO:0000724	double-strand break repair via homologous recombination	7	1
GO:0000725	recombinational repair	6	1
GO:0006303	double-strand break repair via nonhomologous end joining	7	1
GO:0006310	DNA recombination	5	1
GO:0006996	organelle organization	4	1
GO:0007093	mitotic cell cycle checkpoint signaling	4	1
GO:0007095	mitotic G2 DNA damage checkpoint signaling	6	1
GO:0007165	signal transduction	2	1
GO:0007346	regulation of mitotic cell cycle	5	1
GO:0010389	regulation of G2/M transition of mitotic cell cycle	7	1
GO:0010564	regulation of cell cycle process	5	1
GO:0010948	negative regulation of cell cycle process	6	1
GO:0010972	negative regulation of G2/M transition of mitotic cell cycle	8	1
GO:0016043	cellular component organization	3	1
GO:0022402	cell cycle process	2	1
GO:0022414	reproductive process	1	2
GO:0031570	DNA integrity checkpoint signaling	5	1
GO:0032200	telomere organization	6	1
GO:0035556	intracellular signal transduction	3	1
GO:0042138	meiotic DNA double-strand break formation	4	1
GO:0042770	signal transduction in response to DNA damage	4	1
GO:0044773	mitotic DNA damage checkpoint signaling	6	1
GO:0044774	mitotic DNA integrity checkpoint signaling	5	1
GO:0044818	mitotic G2/M transition checkpoint	5	1
GO:0045786	negative regulation of cell cycle	5	1
GO:0045930	negative regulation of mitotic cell cycle	6	1
GO:0048519	negative regulation of biological process	3	1
GO:0048523	negative regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051276	chromosome organization	5	1
GO:0051726	regulation of cell cycle	4	1
GO:0061982	meiosis I cell cycle process	3	1
GO:0065007	biological regulation	1	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	1
GO:0097551	mitochondrial double-strand break repair	7	1
GO:0097552	mitochondrial double-strand break repair via homologous recombination	7	1
GO:1901987	regulation of cell cycle phase transition	6	1
GO:1901988	negative regulation of cell cycle phase transition	7	1
GO:1901990	regulation of mitotic cell cycle phase transition	6	1
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	1
GO:1902749	regulation of cell cycle G2/M phase transition	7	1
GO:1902750	negative regulation of cell cycle G2/M phase transition	8	1
GO:1903046	meiotic cell cycle process	2	1
GO:1903047	mitotic cell cycle process	3	1
GO:0007049	cell cycle	2	1
GO:0051321	meiotic cell cycle	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0004518	nuclease activity	4	8
GO:0004519	endonuclease activity	5	8
GO:0004527	exonuclease activity	5	8
GO:0005488	binding	1	8
GO:0016787	hydrolase activity	2	8
GO:0016788	hydrolase activity, acting on ester bonds	3	8
GO:0030145	manganese ion binding	6	8
GO:0043167	ion binding	2	8
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0046914	transition metal ion binding	5	8
GO:0000014	single-stranded DNA endodeoxyribonuclease activity	6	1
GO:0004520	DNA endonuclease activity	5	2
GO:0004536	DNA nuclease activity	4	2
GO:0140097	catalytic activity, acting on DNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:0004529	DNA exonuclease activity	5	1
GO:0008296	3'-5'-DNA exonuclease activity	7	1
GO:0008408	3'-5' exonuclease activity	6	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	311	315	PF00656	0.556
CLV_C14_Caspase3-7	597	601	PF00656	0.632
CLV_C14_Caspase3-7	832	836	PF00656	0.575
CLV_NRD_NRD_1	161	163	PF00675	0.430
CLV_NRD_NRD_1	200	202	PF00675	0.404
CLV_NRD_NRD_1	24	26	PF00675	0.376
CLV_NRD_NRD_1	303	305	PF00675	0.317
CLV_NRD_NRD_1	405	407	PF00675	0.319
CLV_NRD_NRD_1	496	498	PF00675	0.593
CLV_NRD_NRD_1	73	75	PF00675	0.417
CLV_NRD_NRD_1	775	777	PF00675	0.830
CLV_NRD_NRD_1	827	829	PF00675	0.568
CLV_PCSK_KEX2_1	24	26	PF00082	0.376
CLV_PCSK_KEX2_1	303	305	PF00082	0.317
CLV_PCSK_KEX2_1	405	407	PF00082	0.319
CLV_PCSK_KEX2_1	496	498	PF00082	0.593
CLV_PCSK_KEX2_1	520	522	PF00082	0.527
CLV_PCSK_KEX2_1	692	694	PF00082	0.755
CLV_PCSK_KEX2_1	775	777	PF00082	0.778
CLV_PCSK_KEX2_1	827	829	PF00082	0.568
CLV_PCSK_PC1ET2_1	520	522	PF00082	0.527
CLV_PCSK_PC1ET2_1	692	694	PF00082	0.755
CLV_PCSK_PC7_1	299	305	PF00082	0.317
CLV_PCSK_SKI1_1	397	401	PF00082	0.295
CLV_PCSK_SKI1_1	7	11	PF00082	0.430
CLV_PCSK_SKI1_1	75	79	PF00082	0.451
CLV_Separin_Metazoa	35	39	PF03568	0.430
CLV_Separin_Metazoa	394	398	PF03568	0.556
DEG_SPOP_SBC_1	829	833	PF00917	0.563
DOC_CYCLIN_RxL_1	4	13	PF00134	0.430
DOC_CYCLIN_yCln2_LP_2	395	401	PF00134	0.556
DOC_CYCLIN_yCln2_LP_2	426	432	PF00134	0.579
DOC_MAPK_gen_1	162	170	PF00069	0.376
DOC_MAPK_gen_1	183	192	PF00069	0.376
DOC_MAPK_gen_1	201	209	PF00069	0.376
DOC_MAPK_MEF2A_6	355	363	PF00069	0.517
DOC_MAPK_MEF2A_6	585	593	PF00069	0.536
DOC_PP1_SILK_1	504	509	PF00149	0.699
DOC_PP2B_LxvP_1	246	249	PF13499	0.518
DOC_PP2B_LxvP_1	426	429	PF13499	0.603
DOC_PP2B_PxIxI_1	107	113	PF00149	0.346
DOC_PP4_FxxP_1	105	108	PF00568	0.376
DOC_USP7_MATH_1	249	253	PF00917	0.385
DOC_USP7_MATH_1	421	425	PF00917	0.446
DOC_USP7_MATH_1	502	506	PF00917	0.631
DOC_USP7_MATH_1	565	569	PF00917	0.815
DOC_USP7_MATH_1	611	615	PF00917	0.699
DOC_USP7_MATH_1	675	679	PF00917	0.756
DOC_USP7_MATH_1	755	759	PF00917	0.684
DOC_USP7_MATH_1	829	833	PF00917	0.813
DOC_WW_Pin1_4	288	293	PF00397	0.517
DOC_WW_Pin1_4	821	826	PF00397	0.740
LIG_14-3-3_CanoR_1	162	168	PF00244	0.430
LIG_14-3-3_CanoR_1	284	289	PF00244	0.524
LIG_14-3-3_CanoR_1	303	307	PF00244	0.400
LIG_14-3-3_CanoR_1	38	46	PF00244	0.430
LIG_14-3-3_CanoR_1	609	619	PF00244	0.664
LIG_14-3-3_CanoR_1	68	73	PF00244	0.357
LIG_14-3-3_CanoR_1	693	702	PF00244	0.760
LIG_Actin_WH2_2	147	164	PF00022	0.430
LIG_Actin_WH2_2	382	399	PF00022	0.556
LIG_BIR_II_1	1	5	PF00653	0.581
LIG_BIR_III_4	653	657	PF00653	0.573
LIG_BRCT_BRCA1_1	251	255	PF00533	0.392
LIG_BRCT_BRCA1_1	606	610	PF00533	0.738
LIG_EH1_1	43	51	PF00400	0.376
LIG_FHA_1	128	134	PF00498	0.376
LIG_FHA_1	303	309	PF00498	0.556
LIG_FHA_1	434	440	PF00498	0.534
LIG_FHA_1	723	729	PF00498	0.828
LIG_FHA_1	95	101	PF00498	0.374
LIG_FHA_2	147	153	PF00498	0.430
LIG_FHA_2	29	35	PF00498	0.376
LIG_FHA_2	40	46	PF00498	0.376
LIG_Integrin_RGD_1	25	27	PF01839	0.430
LIG_LIR_Apic_2	370	376	PF02991	0.519
LIG_LIR_Gen_1	165	173	PF02991	0.380
LIG_LIR_Gen_1	252	260	PF02991	0.463
LIG_LIR_Gen_1	27	37	PF02991	0.378
LIG_LIR_Gen_1	364	373	PF02991	0.556
LIG_LIR_Gen_1	579	589	PF02991	0.678
LIG_LIR_Gen_1	82	92	PF02991	0.328
LIG_LIR_Nem_3	138	144	PF02991	0.430
LIG_LIR_Nem_3	165	170	PF02991	0.380
LIG_LIR_Nem_3	252	258	PF02991	0.440
LIG_LIR_Nem_3	27	33	PF02991	0.378
LIG_LIR_Nem_3	277	281	PF02991	0.299
LIG_LIR_Nem_3	3	9	PF02991	0.376
LIG_LIR_Nem_3	364	368	PF02991	0.556
LIG_LIR_Nem_3	493	498	PF02991	0.566
LIG_LIR_Nem_3	71	76	PF02991	0.376
LIG_LIR_Nem_3	82	88	PF02991	0.376
LIG_LYPXL_yS_3	356	359	PF13949	0.556
LIG_NRBOX	482	488	PF00104	0.567
LIG_PALB2_WD40_1	199	207	PF16756	0.376
LIG_PCNA_yPIPBox_3	609	619	PF02747	0.664
LIG_Pex14_2	606	610	PF04695	0.814
LIG_SH2_GRB2like	141	144	PF00017	0.430
LIG_SH2_NCK_1	527	531	PF00017	0.634
LIG_SH2_STAP1	527	531	PF00017	0.582
LIG_SH2_STAT5	141	144	PF00017	0.382
LIG_SH2_STAT5	293	296	PF00017	0.556
LIG_SH2_STAT5	76	79	PF00017	0.376
LIG_SH3_3	109	115	PF00018	0.376
LIG_SH3_3	245	251	PF00018	0.467
LIG_SH3_3	254	260	PF00018	0.418
LIG_SH3_3	386	392	PF00018	0.504
LIG_SH3_3	395	401	PF00018	0.479
LIG_SH3_3	412	418	PF00018	0.483
LIG_SH3_3	426	432	PF00018	0.547
LIG_SH3_3	684	690	PF00018	0.795
LIG_SH3_3	758	764	PF00018	0.562
LIG_SH3_3	819	825	PF00018	0.798
LIG_SUMO_SIM_anti_2	130	138	PF11976	0.376
LIG_SUMO_SIM_par_1	648	653	PF11976	0.569
LIG_TRAF2_1	269	272	PF00917	0.483
LIG_TRAF2_1	32	35	PF00917	0.376
LIG_TRFH_1	288	292	PF08558	0.376
LIG_TYR_ITIM	354	359	PF00017	0.430
LIG_UBA3_1	483	492	PF00899	0.566
LIG_UBA3_1	847	853	PF00899	0.552
LIG_WRC_WIRS_1	30	35	PF05994	0.376
LIG_WRC_WIRS_1	321	326	PF05994	0.430
LIG_WRC_WIRS_1	347	352	PF05994	0.311
LIG_WRC_WIRS_1	578	583	PF05994	0.511
MOD_CDC14_SPxK_1	824	827	PF00782	0.557
MOD_CDK_SPxK_1	821	827	PF00069	0.553
MOD_CDK_SPxxK_3	821	828	PF00069	0.557
MOD_CK1_1	2	8	PF00069	0.523
MOD_CK1_1	29	35	PF00069	0.376
MOD_CK1_1	327	333	PF00069	0.385
MOD_CK1_1	490	496	PF00069	0.568
MOD_CK1_1	604	610	PF00069	0.560
MOD_CK1_1	780	786	PF00069	0.803
MOD_CK1_1	798	804	PF00069	0.466
MOD_CK1_1	852	858	PF00069	0.692
MOD_CK1_1	86	92	PF00069	0.376
MOD_CK2_1	266	272	PF00069	0.481
MOD_CK2_1	28	34	PF00069	0.376
MOD_CK2_1	331	337	PF00069	0.501
MOD_CK2_1	456	462	PF00069	0.376
MOD_Cter_Amidation	199	202	PF01082	0.404
MOD_Cter_Amidation	825	828	PF01082	0.565
MOD_GlcNHglycan	268	271	PF01048	0.543
MOD_GlcNHglycan	369	372	PF01048	0.376
MOD_GlcNHglycan	423	426	PF01048	0.396
MOD_GlcNHglycan	462	466	PF01048	0.376
MOD_GlcNHglycan	606	609	PF01048	0.700
MOD_GlcNHglycan	695	698	PF01048	0.780
MOD_GlcNHglycan	735	741	PF01048	0.559
MOD_GlcNHglycan	757	760	PF01048	0.696
MOD_GlcNHglycan	772	775	PF01048	0.788
MOD_GlcNHglycan	779	782	PF01048	0.748
MOD_GlcNHglycan	789	792	PF01048	0.611
MOD_GlcNHglycan	797	800	PF01048	0.657
MOD_GlcNHglycan	803	806	PF01048	0.721
MOD_GlcNHglycan	819	822	PF01048	0.610
MOD_GlcNHglycan	835	838	PF01048	0.834
MOD_GSK3_1	259	266	PF00069	0.437
MOD_GSK3_1	284	291	PF00069	0.464
MOD_GSK3_1	320	327	PF00069	0.460
MOD_GSK3_1	363	370	PF00069	0.347
MOD_GSK3_1	486	493	PF00069	0.501
MOD_GSK3_1	553	560	PF00069	0.589
MOD_GSK3_1	79	86	PF00069	0.458
MOD_GSK3_1	817	824	PF00069	0.732
MOD_GSK3_1	829	836	PF00069	0.777
MOD_GSK3_1	90	97	PF00069	0.408
MOD_N-GLC_1	502	507	PF02516	0.706
MOD_N-GLC_1	601	606	PF02516	0.555
MOD_N-GLC_1	709	714	PF02516	0.729
MOD_N-GLC_1	79	84	PF02516	0.249
MOD_N-GLC_2	453	455	PF02516	0.501
MOD_NEK2_1	135	140	PF00069	0.380
MOD_NEK2_1	324	329	PF00069	0.385
MOD_NEK2_1	486	491	PF00069	0.553
MOD_NEK2_1	610	615	PF00069	0.717
MOD_NEK2_2	565	570	PF00069	0.561
MOD_PIKK_1	487	493	PF00454	0.559
MOD_PKA_1	162	168	PF00069	0.501
MOD_PKA_2	283	289	PF00069	0.515
MOD_PKA_2	302	308	PF00069	0.212
MOD_PKA_2	557	563	PF00069	0.571
MOD_Plk_1	2	8	PF00069	0.497
MOD_Plk_1	26	32	PF00069	0.376
MOD_Plk_1	502	508	PF00069	0.703
MOD_Plk_1	601	607	PF00069	0.695
MOD_Plk_1	79	85	PF00069	0.263
MOD_Plk_2-3	314	320	PF00069	0.430
MOD_Plk_2-3	331	337	PF00069	0.430
MOD_Plk_4	163	169	PF00069	0.342
MOD_Plk_4	260	266	PF00069	0.438
MOD_Plk_4	320	326	PF00069	0.397
MOD_Plk_4	411	417	PF00069	0.390
MOD_Plk_4	453	459	PF00069	0.376
MOD_Plk_4	490	496	PF00069	0.479
MOD_Plk_4	502	508	PF00069	0.542
MOD_Plk_4	675	681	PF00069	0.691
MOD_Plk_4	68	74	PF00069	0.376
MOD_Plk_4	809	815	PF00069	0.716
MOD_ProDKin_1	288	294	PF00069	0.376
MOD_ProDKin_1	821	827	PF00069	0.745
MOD_SUMO_for_1	401	404	PF00179	0.501
MOD_SUMO_rev_2	54	62	PF00179	0.376
TRG_DiLeu_BaEn_1	242	247	PF01217	0.486
TRG_DiLeu_BaLyEn_6	354	359	PF01217	0.447
TRG_ENDOCYTIC_2	278	281	PF00928	0.386
TRG_ENDOCYTIC_2	356	359	PF00928	0.430
TRG_ENDOCYTIC_2	385	388	PF00928	0.362
TRG_ER_diArg_1	23	25	PF00400	0.430
TRG_ER_diArg_1	302	304	PF00400	0.376
TRG_ER_diArg_1	405	407	PF00400	0.430
TRG_ER_diArg_1	495	497	PF00400	0.607
TRG_ER_diArg_1	638	641	PF00400	0.768
TRG_ER_diArg_1	827	829	PF00400	0.568
TRG_NES_CRM1_1	226	242	PF08389	0.376
TRG_NES_CRM1_1	433	445	PF08389	0.430
TRG_NLS_MonoExtN_4	689	696	PF00514	0.823
TRG_Pf-PMV_PEXEL_1	567	571	PF00026	0.558
TRG_Pf-PMV_PEXEL_1	621	625	PF00026	0.562

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB75	Leptomonas seymouri	63%	99%
A0A3Q8IDJ4	Leishmania donovani	84%	100%
A4I2Z5	Leishmania infantum	84%	100%
E9ADG7	Leishmania major	83%	99%
E9AZ91	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
V5BIP5	Trypanosoma cruzi	61%	100%