Putative trypanothione synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

trypanothione synthetase

Species:

Leishmania braziliensis

UniProt:

A4HFW1_LEIBR

TriTrypDb:

LbrM.27.2010 , LBRM2903_270026600

Length:

652

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HFW1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HFW1

PDB structure(s): 2vob_A , 2vob_B , 2vpm_A , 2vpm_B , 2vps_A

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0016874	ligase activity	2	6
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	6
GO:0016880	acid-ammonia (or amide) ligase activity	4	6
GO:0047479	trypanothione synthase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	121	125	PF00656	0.260
CLV_C14_Caspase3-7	165	169	PF00656	0.513
CLV_C14_Caspase3-7	561	565	PF00656	0.443
CLV_C14_Caspase3-7	89	93	PF00656	0.371
CLV_NRD_NRD_1	109	111	PF00675	0.460
CLV_NRD_NRD_1	222	224	PF00675	0.544
CLV_NRD_NRD_1	540	542	PF00675	0.447
CLV_NRD_NRD_1	64	66	PF00675	0.581
CLV_NRD_NRD_1	69	71	PF00675	0.541
CLV_PCSK_KEX2_1	222	224	PF00082	0.544
CLV_PCSK_KEX2_1	577	579	PF00082	0.418
CLV_PCSK_KEX2_1	64	66	PF00082	0.581
CLV_PCSK_PC1ET2_1	577	579	PF00082	0.418
CLV_PCSK_SKI1_1	383	387	PF00082	0.443
CLV_PCSK_SKI1_1	513	517	PF00082	0.451
CLV_PCSK_SKI1_1	65	69	PF00082	0.581
DEG_APCC_DBOX_1	254	262	PF00400	0.456
DEG_MDM2_SWIB_1	62	69	PF02201	0.344
DOC_CYCLIN_yCln2_LP_2	636	642	PF00134	0.594
DOC_MAPK_gen_1	485	493	PF00069	0.443
DOC_MAPK_JIP1_4	231	237	PF00069	0.376
DOC_MAPK_MEF2A_6	197	206	PF00069	0.569
DOC_PP1_RVXF_1	395	401	PF00149	0.443
DOC_PP2B_LxvP_1	636	639	PF13499	0.430
DOC_PP2B_LxvP_1	73	76	PF13499	0.381
DOC_PP4_FxxP_1	212	215	PF00568	0.538
DOC_PP4_FxxP_1	304	307	PF00568	0.443
DOC_USP7_MATH_1	388	392	PF00917	0.275
DOC_USP7_UBL2_3	163	167	PF12436	0.337
DOC_USP7_UBL2_3	479	483	PF12436	0.443
DOC_USP7_UBL2_3	643	647	PF12436	0.524
DOC_WW_Pin1_4	478	483	PF00397	0.443
DOC_WW_Pin1_4	621	626	PF00397	0.493
LIG_14-3-3_CanoR_1	222	226	PF00244	0.357
LIG_14-3-3_CanoR_1	313	319	PF00244	0.538
LIG_BIR_III_2	124	128	PF00653	0.307
LIG_BRCT_BRCA1_1	208	212	PF00533	0.539
LIG_BRCT_BRCA1_1	325	329	PF00533	0.443
LIG_BRCT_BRCA1_1	375	379	PF00533	0.275
LIG_deltaCOP1_diTrp_1	308	318	PF00928	0.443
LIG_deltaCOP1_diTrp_1	495	502	PF00928	0.443
LIG_FHA_1	114	120	PF00498	0.371
LIG_FHA_1	289	295	PF00498	0.443
LIG_FHA_1	335	341	PF00498	0.443
LIG_FHA_1	393	399	PF00498	0.443
LIG_FHA_2	170	176	PF00498	0.485
LIG_FHA_2	350	356	PF00498	0.495
LIG_FHA_2	559	565	PF00498	0.462
LIG_FHA_2	587	593	PF00498	0.275
LIG_FHA_2	618	624	PF00498	0.454
LIG_LIR_Apic_2	209	215	PF02991	0.536
LIG_LIR_Apic_2	498	504	PF02991	0.443
LIG_LIR_Gen_1	10	19	PF02991	0.361
LIG_LIR_Gen_1	139	149	PF02991	0.381
LIG_LIR_Gen_1	168	179	PF02991	0.507
LIG_LIR_Gen_1	407	418	PF02991	0.443
LIG_LIR_Gen_1	495	505	PF02991	0.443
LIG_LIR_Gen_1	592	603	PF02991	0.443
LIG_LIR_Gen_1	623	633	PF02991	0.443
LIG_LIR_Gen_1	97	104	PF02991	0.378
LIG_LIR_Nem_3	10	14	PF02991	0.422
LIG_LIR_Nem_3	139	144	PF02991	0.381
LIG_LIR_Nem_3	168	174	PF02991	0.498
LIG_LIR_Nem_3	308	314	PF02991	0.443
LIG_LIR_Nem_3	407	413	PF02991	0.443
LIG_LIR_Nem_3	495	500	PF02991	0.443
LIG_LIR_Nem_3	503	509	PF02991	0.355
LIG_LIR_Nem_3	592	598	PF02991	0.443
LIG_LIR_Nem_3	623	629	PF02991	0.443
LIG_LIR_Nem_3	97	102	PF02991	0.378
LIG_MLH1_MIPbox_1	208	212	PF16413	0.370
LIG_MYND_3	1	5	PF01753	0.501
LIG_NRBOX	487	493	PF00104	0.443
LIG_Pex14_1	154	158	PF04695	0.468
LIG_Pex14_1	497	501	PF04695	0.443
LIG_Pex14_2	245	249	PF04695	0.526
LIG_Pex14_2	329	333	PF04695	0.443
LIG_Pex14_2	502	506	PF04695	0.443
LIG_Pex14_2	51	55	PF04695	0.351
LIG_Pex14_2	62	66	PF04695	0.360
LIG_Rb_LxCxE_1	336	355	PF01857	0.275
LIG_Rb_pABgroove_1	426	434	PF01858	0.443
LIG_SH2_CRK	410	414	PF00017	0.443
LIG_SH2_CRK	582	586	PF00017	0.443
LIG_SH2_CRK	595	599	PF00017	0.300
LIG_SH2_GRB2like	33	36	PF00017	0.494
LIG_SH2_GRB2like	520	523	PF00017	0.443
LIG_SH2_NCK_1	595	599	PF00017	0.443
LIG_SH2_PTP2	141	144	PF00017	0.381
LIG_SH2_SRC	25	28	PF00017	0.510
LIG_SH2_SRC	595	598	PF00017	0.443
LIG_SH2_STAP1	158	162	PF00017	0.439
LIG_SH2_STAP1	410	414	PF00017	0.443
LIG_SH2_STAT3	279	282	PF00017	0.443
LIG_SH2_STAT3	414	417	PF00017	0.443
LIG_SH2_STAT5	141	144	PF00017	0.381
LIG_SH2_STAT5	211	214	PF00017	0.468
LIG_SH2_STAT5	219	222	PF00017	0.464
LIG_SH2_STAT5	324	327	PF00017	0.547
LIG_SH2_STAT5	40	43	PF00017	0.481
LIG_SH2_STAT5	410	413	PF00017	0.443
LIG_SH2_STAT5	414	417	PF00017	0.399
LIG_SH2_STAT5	501	504	PF00017	0.443
LIG_SH3_3	186	192	PF00018	0.476
LIG_SH3_3	212	218	PF00018	0.617
LIG_SH3_3	521	527	PF00018	0.443
LIG_SH3_3	619	625	PF00018	0.443
LIG_SH3_3	636	642	PF00018	0.446
LIG_SH3_4	479	486	PF00018	0.443
LIG_SUMO_SIM_par_1	115	124	PF11976	0.260
LIG_TRAF2_1	470	473	PF00917	0.443
LIG_TYR_ITAM	579	598	PF00017	0.443
LIG_UBA3_1	585	590	PF00899	0.443
LIG_UBA3_1	84	88	PF00899	0.362
MOD_CDK_SPK_2	478	483	PF00069	0.443
MOD_CDK_SPxxK_3	478	485	PF00069	0.443
MOD_CK1_1	132	138	PF00069	0.297
MOD_CK1_1	349	355	PF00069	0.485
MOD_CK1_1	392	398	PF00069	0.443
MOD_CK1_1	462	468	PF00069	0.443
MOD_CK1_1	556	562	PF00069	0.441
MOD_CK1_1	617	623	PF00069	0.542
MOD_CK2_1	253	259	PF00069	0.623
MOD_CK2_1	261	267	PF00069	0.343
MOD_CK2_1	349	355	PF00069	0.495
MOD_CK2_1	617	623	PF00069	0.280
MOD_CMANNOS	455	458	PF00535	0.443
MOD_Cter_Amidation	575	578	PF01082	0.418
MOD_GlcNHglycan	325	328	PF01048	0.443
MOD_GlcNHglycan	348	351	PF01048	0.443
MOD_GlcNHglycan	376	379	PF01048	0.506
MOD_GlcNHglycan	391	394	PF01048	0.379
MOD_GlcNHglycan	41	44	PF01048	0.487
MOD_GlcNHglycan	461	464	PF01048	0.508
MOD_GlcNHglycan	561	564	PF01048	0.467
MOD_GlcNHglycan	570	573	PF01048	0.307
MOD_GlcNHglycan	607	610	PF01048	0.446
MOD_GSK3_1	206	213	PF00069	0.393
MOD_GSK3_1	261	268	PF00069	0.511
MOD_GSK3_1	388	395	PF00069	0.311
MOD_GSK3_1	39	46	PF00069	0.424
MOD_GSK3_1	603	610	PF00069	0.351
MOD_GSK3_1	617	624	PF00069	0.485
MOD_N-GLC_1	105	110	PF02516	0.562
MOD_N-GLC_1	334	339	PF02516	0.443
MOD_N-GLC_1	556	561	PF02516	0.379
MOD_NEK2_1	118	123	PF00069	0.260
MOD_NEK2_1	206	211	PF00069	0.406
MOD_NEK2_1	221	226	PF00069	0.339
MOD_NEK2_1	251	256	PF00069	0.575
MOD_NEK2_1	261	266	PF00069	0.339
MOD_NEK2_1	288	293	PF00069	0.462
MOD_NEK2_1	459	464	PF00069	0.443
MOD_NEK2_1	607	612	PF00069	0.521
MOD_PIKK_1	278	284	PF00454	0.443
MOD_PKA_2	221	227	PF00069	0.359
MOD_PKA_2	314	320	PF00069	0.547
MOD_PKA_2	374	380	PF00069	0.542
MOD_Plk_1	154	160	PF00069	0.456
MOD_Plk_1	43	49	PF00069	0.443
MOD_Plk_1	556	562	PF00069	0.379
MOD_Plk_1	614	620	PF00069	0.493
MOD_Plk_4	113	119	PF00069	0.331
MOD_Plk_4	129	135	PF00069	0.249
MOD_Plk_4	206	212	PF00069	0.368
MOD_Plk_4	261	267	PF00069	0.519
MOD_Plk_4	320	326	PF00069	0.443
MOD_Plk_4	349	355	PF00069	0.443
MOD_Plk_4	454	460	PF00069	0.443
MOD_Plk_4	614	620	PF00069	0.443
MOD_ProDKin_1	478	484	PF00069	0.443
MOD_ProDKin_1	621	627	PF00069	0.493
MOD_SUMO_for_1	162	165	PF00179	0.315
MOD_SUMO_for_1	530	533	PF00179	0.443
MOD_SUMO_rev_2	224	232	PF00179	0.408
MOD_SUMO_rev_2	296	301	PF00179	0.443
MOD_SUMO_rev_2	336	343	PF00179	0.443
MOD_SUMO_rev_2	608	618	PF00179	0.443
TRG_DiLeu_BaEn_1	113	118	PF01217	0.381
TRG_ENDOCYTIC_2	141	144	PF00928	0.381
TRG_ENDOCYTIC_2	410	413	PF00928	0.443
TRG_ENDOCYTIC_2	57	60	PF00928	0.381
TRG_ENDOCYTIC_2	582	585	PF00928	0.443
TRG_ENDOCYTIC_2	594	597	PF00928	0.311
TRG_ER_diArg_1	221	223	PF00400	0.567
TRG_ER_diArg_1	255	258	PF00400	0.640
TRG_ER_diArg_1	312	315	PF00400	0.525
TRG_ER_diArg_1	449	452	PF00400	0.443
TRG_ER_diArg_1	64	66	PF00400	0.381

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5Y6	Leptomonas seymouri	76%	100%
A0A0N1PC88	Leptomonas seymouri	27%	91%
A0A0S4J0Y2	Bodo saltans	28%	74%
A0A0S4JSQ6	Bodo saltans	52%	100%
A0A1X0ND08	Trypanosomatidae	27%	91%
A0A1X0NR71	Trypanosomatidae	63%	100%
A0A3Q8IGF4	Leishmania donovani	86%	100%
A0A3Q8IGS6	Leishmania donovani	26%	91%
A0A3R7LYC7	Trypanosoma rangeli	60%	100%
A4I1T8	Leishmania infantum	27%	91%
A4I2Z3	Leishmania infantum	86%	100%
C9ZJE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
E9AXX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	91%
E9AZ89	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
O60993	Crithidia fasciculata	77%	100%
P0AES0	Escherichia coli (strain K12)	34%	100%
P0AES1	Shigella flexneri	34%	100%
P90518	Crithidia fasciculata	26%	91%
Q711P7	Leishmania major	85%	100%
Q9GT49	Trypanosoma cruzi (strain CL Brener)	59%	100%
V5ASH7	Trypanosoma cruzi	28%	91%
V5AYP7	Trypanosoma cruzi	59%	100%