Putative metallopeptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Lys-63-specific deubiquitinase BRCC36, putative

Species:

Leishmania braziliensis

UniProt:

A4HF88_LEIBR

TriTrypDb:

LbrM.26.2360 , LBRM2903_260029500

Length:

261

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	2
GO:0070531	BRCA1-A complex	3	2
GO:0070552	BRISC complex	3	2
GO:0140513	nuclear protein-containing complex	2	2

Expansion

Sequence features

A4HF88

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HF88

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006302	double-strand break repair	6	1
GO:0006508	proteolysis	4	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016570	histone modification	5	1
GO:0016578	histone deubiquitination	6	1
GO:0016579	protein deubiquitination	6	2
GO:0019538	protein metabolic process	3	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0070536	protein K63-linked deubiquitination	7	2
GO:0070537	histone H2A K63-linked deubiquitination	7	1
GO:0070646	protein modification by small protein removal	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0008233	peptidase activity	3	9
GO:0008237	metallopeptidase activity	4	9
GO:0016787	hydrolase activity	2	9
GO:0140096	catalytic activity, acting on a protein	2	9
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0140030	modification-dependent protein binding	3	1
GO:0004843	cysteine-type deubiquitinase activity	5	1
GO:0008234	cysteine-type peptidase activity	4	1
GO:0019783	ubiquitin-like protein peptidase activity	4	1
GO:0101005	deubiquitinase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	227	229	PF00675	0.533
DEG_Nend_Nbox_1	1	3	PF02207	0.459
DEG_SPOP_SBC_1	173	177	PF00917	0.547
DOC_MAPK_gen_1	206	216	PF00069	0.534
DOC_USP7_MATH_1	143	147	PF00917	0.398
DOC_USP7_MATH_1	202	206	PF00917	0.560
DOC_WW_Pin1_4	19	24	PF00397	0.527
DOC_WW_Pin1_4	255	260	PF00397	0.380
LIG_14-3-3_CanoR_1	104	110	PF00244	0.291
LIG_14-3-3_CanoR_1	240	249	PF00244	0.404
LIG_14-3-3_CanoR_1	73	80	PF00244	0.406
LIG_BRCT_BRCA1_1	159	163	PF00533	0.568
LIG_BRCT_BRCA1_1	176	180	PF00533	0.261
LIG_Clathr_ClatBox_1	216	220	PF01394	0.535
LIG_EH1_1	216	224	PF00400	0.451
LIG_FHA_1	14	20	PF00498	0.530
LIG_FHA_1	174	180	PF00498	0.446
LIG_FHA_1	80	86	PF00498	0.328
LIG_FHA_2	173	179	PF00498	0.406
LIG_FHA_2	192	198	PF00498	0.500
LIG_FHA_2	225	231	PF00498	0.534
LIG_LIR_Gen_1	160	171	PF02991	0.561
LIG_LIR_Nem_3	108	112	PF02991	0.281
LIG_LIR_Nem_3	114	120	PF02991	0.356
LIG_LIR_Nem_3	160	166	PF02991	0.539
LIG_LIR_Nem_3	177	183	PF02991	0.387
LIG_SH2_SRC	112	115	PF00017	0.211
LIG_SH2_STAT5	112	115	PF00017	0.368
LIG_SH2_STAT5	40	43	PF00017	0.502
LIG_SH2_STAT5	83	86	PF00017	0.421
LIG_SH3_3	147	153	PF00018	0.563
LIG_SH3_3	220	226	PF00018	0.474
LIG_SH3_3	89	95	PF00018	0.382
LIG_SUMO_SIM_par_1	99	105	PF11976	0.395
MOD_CK1_1	60	66	PF00069	0.536
MOD_CK2_1	172	178	PF00069	0.443
MOD_CK2_1	191	197	PF00069	0.511
MOD_CK2_1	224	230	PF00069	0.500
MOD_DYRK1A_RPxSP_1	255	259	PF00069	0.370
MOD_GlcNHglycan	114	117	PF01048	0.399
MOD_GlcNHglycan	242	245	PF01048	0.490
MOD_GlcNHglycan	252	255	PF01048	0.415
MOD_GlcNHglycan	30	33	PF01048	0.666
MOD_GlcNHglycan	58	62	PF01048	0.548
MOD_GlcNHglycan	65	68	PF01048	0.436
MOD_GSK3_1	120	127	PF00069	0.385
MOD_GSK3_1	13	20	PF00069	0.609
MOD_GSK3_1	191	198	PF00069	0.512
MOD_GSK3_1	63	70	PF00069	0.488
MOD_GSK3_1	75	82	PF00069	0.397
MOD_N-GLC_1	131	136	PF02516	0.515
MOD_NEK2_1	120	125	PF00069	0.369
MOD_NEK2_1	239	244	PF00069	0.451
MOD_NEK2_1	250	255	PF00069	0.553
MOD_NEK2_1	27	32	PF00069	0.509
MOD_PIKK_1	102	108	PF00454	0.332
MOD_PKA_2	195	201	PF00069	0.578
MOD_PKA_2	239	245	PF00069	0.491
MOD_PKA_2	72	78	PF00069	0.323
MOD_Plk_4	120	126	PF00069	0.470
MOD_Plk_4	96	102	PF00069	0.338
MOD_ProDKin_1	19	25	PF00069	0.524
MOD_ProDKin_1	255	261	PF00069	0.393
MOD_SUMO_for_1	33	36	PF00179	0.618
MOD_SUMO_rev_2	29	39	PF00179	0.570
TRG_DiLeu_BaLyEn_6	234	239	PF01217	0.552
TRG_Pf-PMV_PEXEL_1	181	185	PF00026	0.397
TRG_Pf-PMV_PEXEL_1	209	213	PF00026	0.405
TRG_Pf-PMV_PEXEL_1	248	252	PF00026	0.330

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFL1	Leptomonas seymouri	57%	78%
A0A0S4IX58	Bodo saltans	35%	100%
A0A1X0P845	Trypanosomatidae	42%	100%
B2RYM5	Rattus norvegicus	31%	90%
C9ZX24	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	94%
E9AYM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	78%
P46737	Mus musculus	32%	90%
Q4Q8U8	Leishmania major	75%	100%
V5BGS3	Trypanosoma cruzi	41%	86%