Putative nitrilase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative nitrilase

Gene product:

nitrilase, putative

Species:

Leishmania braziliensis

UniProt:

A4HF72_LEIBR

TriTrypDb:

LbrM.26.2200 , LBRM2903_260026900

Length:

279

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 10
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4HF72

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HF72

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0006082	organic acid metabolic process	3	1
GO:0006107	oxaloacetate metabolic process	7	1
GO:0006520	amino acid metabolic process	3	1
GO:0006528	asparagine metabolic process	6	1
GO:0006541	glutamine metabolic process	6	1
GO:0009064	glutamine family amino acid metabolic process	5	1
GO:0009066	aspartate family amino acid metabolic process	5	1
GO:0009987	cellular process	1	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0043436	oxoacid metabolic process	4	1
GO:0043648	dicarboxylic acid metabolic process	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044281	small molecule metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901605	alpha-amino acid metabolic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0000257	nitrilase activity	5	7
GO:0003824	catalytic activity	1	12
GO:0016787	hydrolase activity	2	12
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	12
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	12
GO:0016815	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in nitriles	4	7
GO:0080061	indole-3-acetonitrile nitrilase activity	6	7
GO:0050152	omega-amidase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	114	116	PF00675	0.346
CLV_NRD_NRD_1	22	24	PF00675	0.366
CLV_PCSK_KEX2_1	34	36	PF00082	0.390
CLV_PCSK_PC1ET2_1	34	36	PF00082	0.413
DEG_APCC_DBOX_1	268	276	PF00400	0.507
DOC_MAPK_gen_1	23	30	PF00069	0.424
DOC_MAPK_gen_1	34	41	PF00069	0.375
DOC_MAPK_MEF2A_6	151	158	PF00069	0.375
DOC_PP4_FxxP_1	46	49	PF00568	0.356
DOC_USP7_MATH_1	89	93	PF00917	0.432
DOC_USP7_UBL2_3	34	38	PF12436	0.442
DOC_WW_Pin1_4	208	213	PF00397	0.356
LIG_14-3-3_CanoR_1	121	129	PF00244	0.325
LIG_14-3-3_CanoR_1	57	64	PF00244	0.491
LIG_14-3-3_CanoR_1	96	106	PF00244	0.250
LIG_Actin_WH2_2	95	113	PF00022	0.441
LIG_BIR_II_1	1	5	PF00653	0.517
LIG_BRCT_BRCA1_1	100	104	PF00533	0.390
LIG_FHA_1	122	128	PF00498	0.387
LIG_FHA_1	172	178	PF00498	0.347
LIG_FHA_2	214	220	PF00498	0.441
LIG_LIR_Apic_2	44	49	PF02991	0.356
LIG_LIR_Gen_1	67	76	PF02991	0.360
LIG_LIR_Nem_3	159	165	PF02991	0.356
LIG_LIR_Nem_3	174	179	PF02991	0.356
LIG_LIR_Nem_3	243	248	PF02991	0.387
LIG_LIR_Nem_3	52	58	PF02991	0.367
LIG_LYPXL_yS_3	162	165	PF13949	0.390
LIG_PTB_Apo_2	200	207	PF02174	0.371
LIG_PTB_Apo_2	267	274	PF02174	0.418
LIG_PTB_Phospho_1	200	206	PF10480	0.476
LIG_PTB_Phospho_1	267	273	PF10480	0.415
LIG_SH2_PTP2	206	209	PF00017	0.476
LIG_SH2_STAP1	273	277	PF00017	0.490
LIG_SH2_STAP1	58	62	PF00017	0.371
LIG_SH2_STAT3	169	172	PF00017	0.433
LIG_SH2_STAT5	206	209	PF00017	0.426
LIG_SH2_STAT5	220	223	PF00017	0.267
LIG_SH2_STAT5	273	276	PF00017	0.606
LIG_SH2_STAT5	54	57	PF00017	0.371
LIG_SH3_3	183	189	PF00018	0.371
LIG_SH3_3	225	231	PF00018	0.362
LIG_SUMO_SIM_anti_2	37	44	PF11976	0.414
LIG_SUMO_SIM_par_1	2	8	PF11976	0.313
LIG_SxIP_EBH_1	259	268	PF03271	0.342
LIG_TYR_ITIM	160	165	PF00017	0.418
MOD_CDC14_SPxK_1	211	214	PF00782	0.356
MOD_CDK_SPxK_1	208	214	PF00069	0.356
MOD_CK1_1	100	106	PF00069	0.476
MOD_CK1_1	253	259	PF00069	0.482
MOD_CK1_1	3	9	PF00069	0.543
MOD_CK2_1	142	148	PF00069	0.476
MOD_CK2_1	213	219	PF00069	0.413
MOD_CK2_1	84	90	PF00069	0.356
MOD_GlcNHglycan	135	138	PF01048	0.410
MOD_GlcNHglycan	215	219	PF01048	0.441
MOD_GlcNHglycan	251	255	PF01048	0.419
MOD_GlcNHglycan	90	94	PF01048	0.504
MOD_GSK3_1	253	260	PF00069	0.323
MOD_N-GLC_1	65	70	PF02516	0.476
MOD_N-GLC_1	98	103	PF02516	0.371
MOD_NEK2_1	5	10	PF00069	0.262
MOD_NEK2_1	97	102	PF00069	0.350
MOD_PK_1	77	83	PF00069	0.413
MOD_PKA_1	34	40	PF00069	0.413
MOD_PKA_2	213	219	PF00069	0.413
MOD_PKA_2	34	40	PF00069	0.413
MOD_PKA_2	56	62	PF00069	0.511
MOD_Plk_1	139	145	PF00069	0.480
MOD_Plk_1	250	256	PF00069	0.441
MOD_Plk_1	65	71	PF00069	0.432
MOD_Plk_1	98	104	PF00069	0.390
MOD_Plk_4	172	178	PF00069	0.349
MOD_Plk_4	223	229	PF00069	0.361
MOD_Plk_4	77	83	PF00069	0.413
MOD_ProDKin_1	208	214	PF00069	0.356
MOD_SUMO_rev_2	241	250	PF00179	0.503
TRG_DiLeu_BaEn_2	240	246	PF01217	0.476
TRG_ENDOCYTIC_2	162	165	PF00928	0.371
TRG_ENDOCYTIC_2	206	209	PF00928	0.476
TRG_ER_diArg_1	259	262	PF00400	0.565

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1H1	Leptomonas seymouri	79%	100%
A0A0S4ILR2	Bodo saltans	56%	98%
A0A140NCB4	Escherichia coli (strain B / BL21-DE3)	26%	100%
A0A140NDS5	Escherichia coli (strain B / BL21-DE3)	25%	100%
A0A1X0P8A8	Trypanosomatidae	72%	100%
A0A3S7X065	Leishmania donovani	89%	100%
A0A422P3W9	Trypanosoma rangeli	64%	100%
A4I2F5	Leishmania infantum	88%	100%
C9ZX42	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E9AYK8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
K2QXC4	Macrophomina phaseolina (strain MS6)	24%	81%
O31664	Bacillus subtilis (strain 168)	29%	100%
O59829	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
O94660	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
P0DP65	Staphylococcus aureus (strain 04-02981)	30%	100%
P0DP66	Synechocystis sp. (strain PCC 6803 / GT-S)	29%	100%
P0DP67	Yersinia enterocolitica	25%	100%
P0DP68	Yersinia enterocolitica	25%	98%
P46010	Arabidopsis thaliana	26%	81%
P47016	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	91%
P49954	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	96%
P55175	Synechocystis sp. (strain PCC 6803 / Kazusa)	29%	100%
P55176	Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CFBP 6595 / CHA0)	28%	98%
P55177	Staphylococcus aureus	29%	100%
P55178	Staphylococcus lugdunensis	27%	100%
P58054	Escherichia coli O157:H7	26%	100%
P9WJ00	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	26%	100%
P9WJ01	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	26%	100%
Q10166	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	47%	87%
Q28IE5	Xenopus tropicalis	47%	100%
Q2T9R6	Bos taurus	47%	100%
Q32LH4	Bos taurus	31%	85%
Q3HVN1	Solanum tuberosum	23%	93%
Q47679	Escherichia coli (strain K12)	25%	100%
Q497B0	Rattus norvegicus	49%	100%
Q4Q8W4	Leishmania major	89%	100%
Q4VBV9	Danio rerio	48%	100%
Q54JM9	Dictyostelium discoideum	46%	85%
Q557J5	Dictyostelium discoideum	31%	96%
Q5NHL7	Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)	23%	98%
Q5R4L6	Pongo abelii	46%	100%
Q6INI7	Xenopus laevis	48%	100%
Q6IR61	Xenopus laevis	47%	100%
Q75SP7	Pseudomonas sp.	25%	100%
Q7TQ94	Rattus norvegicus	30%	85%
Q86X76	Homo sapiens	30%	85%
Q8RUF8	Arabidopsis thaliana	44%	76%
Q8VDK1	Mus musculus	32%	86%
Q8VYF5	Arabidopsis thaliana	23%	93%
Q93NG1	Paenarthrobacter nicotinovorans	29%	95%
Q93XI4	Oryza sativa subsp. japonica	24%	93%
Q94JV5	Arabidopsis thaliana	28%	91%
Q9JHW2	Mus musculus	48%	100%
Q9NQR4	Homo sapiens	46%	100%
Q9UYV8	Pyrococcus abyssi (strain GE5 / Orsay)	28%	100%
Q9XGI9	Solanum lycopersicum	23%	93%
V5B8Z7	Trypanosoma cruzi	63%	100%
V5IPE4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	25%	85%