Putative sphingosine kinase A, B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Sphingosine kinase

Species:

Leishmania braziliensis

UniProt:

A4HES6_LEIBR

TriTrypDb:

LbrM.26.0720 , LBRM2903_260012000

Length:

817

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0016020	membrane	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HES6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HES6

Function

Biological processes
Term	Name	Level	Count
GO:0006066	alcohol metabolic process	3	1
GO:0006629	lipid metabolic process	3	1
GO:0006643	membrane lipid metabolic process	4	1
GO:0006665	sphingolipid metabolic process	4	1
GO:0006670	sphingosine metabolic process	6	1
GO:0006793	phosphorus metabolic process	3	4
GO:0006796	phosphate-containing compound metabolic process	4	4
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	4
GO:0008610	lipid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	4
GO:0016310	phosphorylation	5	4
GO:0019751	polyol metabolic process	4	1
GO:0030148	sphingolipid biosynthetic process	5	1
GO:0034311	diol metabolic process	5	1
GO:0034312	diol biosynthetic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046165	alcohol biosynthetic process	4	1
GO:0046173	polyol biosynthetic process	5	1
GO:0046467	membrane lipid biosynthetic process	4	1
GO:0046512	sphingosine biosynthetic process	5	1
GO:0046519	sphingoid metabolic process	5	1
GO:0046520	sphingoid biosynthetic process	6	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901615	organic hydroxy compound metabolic process	3	1
GO:1901617	organic hydroxy compound biosynthetic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0001727	lipid kinase activity	5	5
GO:0003824	catalytic activity	1	10
GO:0008481	sphinganine kinase activity	5	5
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	10
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	10
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	6
GO:0017050	D-erythro-sphingosine kinase activity	5	10
GO:0003951	NAD+ kinase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	146	150	PF00656	0.493
CLV_C14_Caspase3-7	790	794	PF00656	0.357
CLV_NRD_NRD_1	138	140	PF00675	0.508
CLV_NRD_NRD_1	248	250	PF00675	0.448
CLV_NRD_NRD_1	288	290	PF00675	0.294
CLV_NRD_NRD_1	482	484	PF00675	0.514
CLV_NRD_NRD_1	749	751	PF00675	0.276
CLV_PCSK_KEX2_1	138	140	PF00082	0.508
CLV_PCSK_KEX2_1	248	250	PF00082	0.448
CLV_PCSK_KEX2_1	288	290	PF00082	0.293
CLV_PCSK_KEX2_1	476	478	PF00082	0.471
CLV_PCSK_KEX2_1	482	484	PF00082	0.411
CLV_PCSK_KEX2_1	749	751	PF00082	0.337
CLV_PCSK_PC1ET2_1	476	478	PF00082	0.356
CLV_PCSK_PC1ET2_1	482	484	PF00082	0.352
CLV_PCSK_PC7_1	472	478	PF00082	0.433
CLV_PCSK_SKI1_1	232	236	PF00082	0.360
CLV_PCSK_SKI1_1	290	294	PF00082	0.436
CLV_PCSK_SKI1_1	298	302	PF00082	0.443
CLV_PCSK_SKI1_1	354	358	PF00082	0.499
CLV_PCSK_SKI1_1	38	42	PF00082	0.464
CLV_PCSK_SKI1_1	53	57	PF00082	0.487
CLV_PCSK_SKI1_1	546	550	PF00082	0.384
CLV_PCSK_SKI1_1	591	595	PF00082	0.449
CLV_PCSK_SKI1_1	684	688	PF00082	0.294
CLV_PCSK_SKI1_1	87	91	PF00082	0.519
DEG_APCC_DBOX_1	287	295	PF00400	0.472
DEG_SPOP_SBC_1	646	650	PF00917	0.490
DOC_CYCLIN_RxL_1	229	238	PF00134	0.358
DOC_CYCLIN_yCln2_LP_2	260	266	PF00134	0.288
DOC_CYCLIN_yCln2_LP_2	56	62	PF00134	0.405
DOC_CYCLIN_yCln2_LP_2	724	730	PF00134	0.408
DOC_MAPK_gen_1	205	214	PF00069	0.318
DOC_MAPK_gen_1	229	237	PF00069	0.351
DOC_MAPK_gen_1	51	60	PF00069	0.511
DOC_MAPK_gen_1	749	758	PF00069	0.300
DOC_MAPK_gen_1	777	785	PF00069	0.395
DOC_MAPK_gen_1	803	813	PF00069	0.408
DOC_MAPK_HePTP_8	202	214	PF00069	0.347
DOC_MAPK_MEF2A_6	205	214	PF00069	0.236
DOC_MAPK_MEF2A_6	229	237	PF00069	0.377
DOC_MAPK_MEF2A_6	51	60	PF00069	0.519
DOC_MAPK_MEF2A_6	546	554	PF00069	0.448
DOC_MAPK_MEF2A_6	777	785	PF00069	0.463
DOC_PP1_RVXF_1	544	550	PF00149	0.398
DOC_PP1_RVXF_1	85	91	PF00149	0.557
DOC_PP1_SILK_1	591	596	PF00149	0.291
DOC_PP2B_LxvP_1	56	59	PF13499	0.462
DOC_PP2B_LxvP_1	724	727	PF13499	0.408
DOC_PP2B_LxvP_1	9	12	PF13499	0.471
DOC_SPAK_OSR1_1	574	578	PF12202	0.417
DOC_USP7_MATH_1	148	152	PF00917	0.749
DOC_USP7_MATH_1	310	314	PF00917	0.706
DOC_USP7_MATH_1	387	391	PF00917	0.556
DOC_USP7_MATH_1	421	425	PF00917	0.402
DOC_USP7_MATH_1	548	552	PF00917	0.332
DOC_USP7_MATH_1	598	602	PF00917	0.498
DOC_USP7_MATH_1	646	650	PF00917	0.596
DOC_USP7_MATH_1	729	733	PF00917	0.337
DOC_USP7_MATH_1	798	802	PF00917	0.408
DOC_USP7_MATH_1	91	95	PF00917	0.387
DOC_USP7_MATH_2	346	352	PF00917	0.490
DOC_USP7_UBL2_3	605	609	PF12436	0.522
DOC_WW_Pin1_4	306	311	PF00397	0.598
DOC_WW_Pin1_4	32	37	PF00397	0.366
DOC_WW_Pin1_4	342	347	PF00397	0.484
DOC_WW_Pin1_4	457	462	PF00397	0.491
DOC_WW_Pin1_4	637	642	PF00397	0.435
LIG_14-3-3_CanoR_1	121	129	PF00244	0.586
LIG_14-3-3_CanoR_1	138	147	PF00244	0.599
LIG_14-3-3_CanoR_1	192	202	PF00244	0.552
LIG_14-3-3_CanoR_1	239	243	PF00244	0.358
LIG_14-3-3_CanoR_1	28	33	PF00244	0.553
LIG_14-3-3_CanoR_1	295	301	PF00244	0.515
LIG_14-3-3_CanoR_1	470	478	PF00244	0.559
LIG_14-3-3_CanoR_1	508	514	PF00244	0.566
LIG_14-3-3_CanoR_1	615	624	PF00244	0.524
LIG_14-3-3_CanoR_1	670	678	PF00244	0.457
LIG_Actin_WH2_2	12	27	PF00022	0.359
LIG_Actin_WH2_2	704	719	PF00022	0.209
LIG_APCC_ABBA_1	678	683	PF00400	0.512
LIG_APCC_ABBAyCdc20_2	354	360	PF00400	0.523
LIG_APCC_ABBAyCdc20_2	684	690	PF00400	0.293
LIG_BRCT_BRCA1_1	140	144	PF00533	0.680
LIG_BRCT_BRCA1_1	313	317	PF00533	0.493
LIG_BRCT_BRCA1_1	526	530	PF00533	0.282
LIG_BRCT_BRCA1_1	550	554	PF00533	0.336
LIG_eIF4E_1	681	687	PF01652	0.501
LIG_FHA_1	126	132	PF00498	0.744
LIG_FHA_1	299	305	PF00498	0.526
LIG_FHA_1	307	313	PF00498	0.689
LIG_FHA_1	386	392	PF00498	0.446
LIG_FHA_1	50	56	PF00498	0.603
LIG_FHA_1	83	89	PF00498	0.623
LIG_FHA_1	95	101	PF00498	0.483
LIG_FHA_2	106	112	PF00498	0.629
LIG_FHA_2	144	150	PF00498	0.665
LIG_FHA_2	343	349	PF00498	0.520
LIG_FHA_2	392	398	PF00498	0.532
LIG_FHA_2	458	464	PF00498	0.538
LIG_FHA_2	512	518	PF00498	0.566
LIG_FHA_2	624	630	PF00498	0.439
LIG_FHA_2	66	72	PF00498	0.420
LIG_FHA_2	680	686	PF00498	0.391
LIG_Integrin_RGD_1	705	707	PF01839	0.437
LIG_LIR_Apic_2	517	521	PF02991	0.379
LIG_LIR_Gen_1	314	322	PF02991	0.574
LIG_LIR_Gen_1	707	716	PF02991	0.280
LIG_LIR_Gen_1	737	745	PF02991	0.380
LIG_LIR_Nem_3	141	147	PF02991	0.679
LIG_LIR_Nem_3	241	245	PF02991	0.237
LIG_LIR_Nem_3	314	320	PF02991	0.575
LIG_LIR_Nem_3	737	743	PF02991	0.380
LIG_LIR_Nem_3	771	775	PF02991	0.408
LIG_PTB_Apo_2	569	576	PF02174	0.377
LIG_SH2_CRK	518	522	PF00017	0.363
LIG_SH2_CRK	581	585	PF00017	0.456
LIG_SH2_CRK	590	594	PF00017	0.387
LIG_SH2_NCK_1	147	151	PF00017	0.490
LIG_SH2_NCK_1	518	522	PF00017	0.363
LIG_SH2_SRC	147	150	PF00017	0.494
LIG_SH2_STAT3	166	169	PF00017	0.528
LIG_SH2_STAT5	166	169	PF00017	0.421
LIG_SH2_STAT5	245	248	PF00017	0.306
LIG_SH2_STAT5	256	259	PF00017	0.369
LIG_SH2_STAT5	596	599	PF00017	0.398
LIG_SH2_STAT5	681	684	PF00017	0.510
LIG_SH2_STAT5	740	743	PF00017	0.408
LIG_SH2_STAT5	772	775	PF00017	0.293
LIG_SUMO_SIM_anti_2	431	436	PF11976	0.394
LIG_SUMO_SIM_par_1	433	439	PF11976	0.497
LIG_TRAF2_1	390	393	PF00917	0.394
LIG_UBA3_1	210	217	PF00899	0.353
LIG_UBA3_1	454	458	PF00899	0.338
LIG_UBA3_1	583	591	PF00899	0.472
LIG_UBA3_1	593	602	PF00899	0.336
MOD_CDC14_SPxK_1	35	38	PF00782	0.403
MOD_CDK_SPxK_1	32	38	PF00069	0.377
MOD_CK1_1	103	109	PF00069	0.726
MOD_CK1_1	142	148	PF00069	0.589
MOD_CK1_1	176	182	PF00069	0.452
MOD_CK1_1	184	190	PF00069	0.462
MOD_CK1_1	215	221	PF00069	0.293
MOD_CK1_1	238	244	PF00069	0.411
MOD_CK1_1	265	271	PF00069	0.243
MOD_CK1_1	299	305	PF00069	0.522
MOD_CK1_1	385	391	PF00069	0.691
MOD_CK1_1	49	55	PF00069	0.579
MOD_CK1_1	535	541	PF00069	0.568
MOD_CK1_1	645	651	PF00069	0.658
MOD_CK1_1	662	668	PF00069	0.700
MOD_CK1_1	761	767	PF00069	0.209
MOD_CK1_1	94	100	PF00069	0.603
MOD_CK2_1	322	328	PF00069	0.654
MOD_CK2_1	342	348	PF00069	0.427
MOD_CK2_1	386	392	PF00069	0.629
MOD_CK2_1	433	439	PF00069	0.436
MOD_CK2_1	457	463	PF00069	0.499
MOD_CK2_1	483	489	PF00069	0.318
MOD_CK2_1	511	517	PF00069	0.405
MOD_CK2_1	623	629	PF00069	0.573
MOD_CK2_1	65	71	PF00069	0.404
MOD_CK2_1	679	685	PF00069	0.428
MOD_CK2_1	789	795	PF00069	0.318
MOD_Cter_Amidation	747	750	PF01082	0.302
MOD_GlcNHglycan	105	108	PF01048	0.705
MOD_GlcNHglycan	132	135	PF01048	0.559
MOD_GlcNHglycan	186	189	PF01048	0.587
MOD_GlcNHglycan	217	220	PF01048	0.305
MOD_GlcNHglycan	362	365	PF01048	0.613
MOD_GlcNHglycan	384	387	PF01048	0.777
MOD_GlcNHglycan	423	426	PF01048	0.390
MOD_GlcNHglycan	473	476	PF01048	0.421
MOD_GlcNHglycan	48	51	PF01048	0.441
MOD_GlcNHglycan	672	675	PF01048	0.544
MOD_GlcNHglycan	763	766	PF01048	0.366
MOD_GSK3_1	117	124	PF00069	0.564
MOD_GSK3_1	138	145	PF00069	0.614
MOD_GSK3_1	148	155	PF00069	0.467
MOD_GSK3_1	265	272	PF00069	0.209
MOD_GSK3_1	28	35	PF00069	0.424
MOD_GSK3_1	298	305	PF00069	0.502
MOD_GSK3_1	306	313	PF00069	0.541
MOD_GSK3_1	340	347	PF00069	0.512
MOD_GSK3_1	382	389	PF00069	0.716
MOD_GSK3_1	41	48	PF00069	0.510
MOD_GSK3_1	611	618	PF00069	0.520
MOD_GSK3_1	642	649	PF00069	0.486
MOD_GSK3_1	658	665	PF00069	0.733
MOD_GSK3_1	670	677	PF00069	0.425
MOD_GSK3_1	798	805	PF00069	0.375
MOD_GSK3_1	96	103	PF00069	0.534
MOD_LATS_1	119	125	PF00433	0.544
MOD_LATS_1	137	143	PF00433	0.450
MOD_N-GLC_1	184	189	PF02516	0.326
MOD_N-GLC_1	322	327	PF02516	0.587
MOD_N-GLC_1	662	667	PF02516	0.737
MOD_NEK2_1	100	105	PF00069	0.666
MOD_NEK2_1	117	122	PF00069	0.640
MOD_NEK2_1	152	157	PF00069	0.541
MOD_NEK2_1	19	24	PF00069	0.474
MOD_NEK2_1	213	218	PF00069	0.288
MOD_NEK2_1	235	240	PF00069	0.318
MOD_NEK2_1	41	46	PF00069	0.555
MOD_NEK2_1	416	421	PF00069	0.364
MOD_NEK2_1	550	555	PF00069	0.339
MOD_NEK2_1	647	652	PF00069	0.554
MOD_NEK2_1	807	812	PF00069	0.408
MOD_NEK2_2	563	568	PF00069	0.488
MOD_NEK2_2	623	628	PF00069	0.529
MOD_NEK2_2	700	705	PF00069	0.474
MOD_NEK2_2	798	803	PF00069	0.408
MOD_PIKK_1	193	199	PF00454	0.278
MOD_PIKK_1	483	489	PF00454	0.605
MOD_PIKK_1	648	654	PF00454	0.593
MOD_PKA_1	138	144	PF00069	0.503
MOD_PKA_1	483	489	PF00069	0.494
MOD_PKA_2	137	143	PF00069	0.773
MOD_PKA_2	238	244	PF00069	0.364
MOD_PKA_2	41	47	PF00069	0.480
MOD_PKA_2	471	477	PF00069	0.497
MOD_PKA_2	669	675	PF00069	0.490
MOD_PKA_2	802	808	PF00069	0.408
MOD_PKA_2	94	100	PF00069	0.646
MOD_PKB_1	613	621	PF00069	0.365
MOD_Plk_1	148	154	PF00069	0.619
MOD_Plk_1	19	25	PF00069	0.320
MOD_Plk_1	497	503	PF00069	0.386
MOD_Plk_1	82	88	PF00069	0.531
MOD_Plk_2-3	433	439	PF00069	0.456
MOD_Plk_4	19	25	PF00069	0.425
MOD_Plk_4	265	271	PF00069	0.408
MOD_Plk_4	440	446	PF00069	0.363
MOD_Plk_4	525	531	PF00069	0.392
MOD_Plk_4	771	777	PF00069	0.215
MOD_Plk_4	96	102	PF00069	0.460
MOD_ProDKin_1	306	312	PF00069	0.604
MOD_ProDKin_1	32	38	PF00069	0.377
MOD_ProDKin_1	342	348	PF00069	0.487
MOD_ProDKin_1	457	463	PF00069	0.499
MOD_ProDKin_1	637	643	PF00069	0.443
MOD_SUMO_for_1	502	505	PF00179	0.384
TRG_DiLeu_BaLyEn_6	230	235	PF01217	0.358
TRG_DiLeu_BaLyEn_6	58	63	PF01217	0.281
TRG_ENDOCYTIC_2	581	584	PF00928	0.371
TRG_ENDOCYTIC_2	590	593	PF00928	0.360
TRG_ENDOCYTIC_2	740	743	PF00928	0.383
TRG_ER_diArg_1	167	170	PF00400	0.440
TRG_ER_diArg_1	470	473	PF00400	0.612
TRG_ER_diArg_1	499	502	PF00400	0.532
TRG_ER_diArg_1	612	615	PF00400	0.412
TRG_ER_diArg_1	749	752	PF00400	0.236
TRG_Pf-PMV_PEXEL_1	595	600	PF00026	0.375
TRG_Pf-PMV_PEXEL_1	684	689	PF00026	0.292
TRG_Pf-PMV_PEXEL_1	717	721	PF00026	0.288

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P530	Leptomonas seymouri	56%	84%
A0A1X0NSV1	Trypanosomatidae	30%	100%
A0A3Q8IGX7	Leishmania donovani	74%	87%
A0A422NN92	Trypanosoma rangeli	32%	100%
A4I203	Leishmania infantum	74%	87%
C9ZS12	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AY50	Leishmania mexicana (strain MHOM/GT/2001/U1103)	71%	89%
Q4Q9C3	Leishmania major	72%	100%