Thioredoxin domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase, putative

Species:

Leishmania braziliensis

UniProt:

A4HES1_LEIBR

TriTrypDb:

LbrM.26.0670 , LBRM2903_260011500

Length:

379

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 9, no: 2
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005794	Golgi apparatus	5	1

Expansion

Sequence features

A4HES1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HES1

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	1
GO:0009987	cellular process	1	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016853	isomerase activity	2	11
GO:0003756	protein disulfide isomerase activity	3	9
GO:0016860	intramolecular oxidoreductase activity	3	9
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	9
GO:0140096	catalytic activity, acting on a protein	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	250	252	PF00675	0.453
CLV_PCSK_KEX2_1	249	251	PF00082	0.496
CLV_PCSK_KEX2_1	263	265	PF00082	0.342
CLV_PCSK_KEX2_1	362	364	PF00082	0.460
CLV_PCSK_PC1ET2_1	263	265	PF00082	0.437
CLV_PCSK_PC1ET2_1	362	364	PF00082	0.491
CLV_PCSK_SKI1_1	174	178	PF00082	0.362
CLV_PCSK_SKI1_1	200	204	PF00082	0.410
CLV_PCSK_SKI1_1	291	295	PF00082	0.553
CLV_PCSK_SKI1_1	312	316	PF00082	0.552
CLV_PCSK_SKI1_1	50	54	PF00082	0.455
CLV_Separin_Metazoa	122	126	PF03568	0.245
DEG_APCC_DBOX_1	250	258	PF00400	0.245
DEG_Nend_UBRbox_3	1	3	PF02207	0.591
DOC_CKS1_1	183	188	PF01111	0.245
DOC_CKS1_1	229	234	PF01111	0.245
DOC_CKS1_1	56	61	PF01111	0.226
DOC_MAPK_gen_1	48	56	PF00069	0.498
DOC_MAPK_HePTP_8	89	101	PF00069	0.245
DOC_MAPK_MEF2A_6	141	150	PF00069	0.376
DOC_MAPK_MEF2A_6	3	12	PF00069	0.633
DOC_MAPK_MEF2A_6	92	101	PF00069	0.245
DOC_PP2B_LxvP_1	66	69	PF13499	0.245
DOC_PP4_FxxP_1	115	118	PF00568	0.245
DOC_PP4_FxxP_1	180	183	PF00568	0.351
DOC_PP4_FxxP_1	56	59	PF00568	0.351
DOC_USP7_MATH_1	18	22	PF00917	0.591
DOC_USP7_UBL2_3	137	141	PF12436	0.245
DOC_USP7_UBL2_3	221	225	PF12436	0.461
DOC_USP7_UBL2_3	263	267	PF12436	0.334
DOC_WW_Pin1_4	107	112	PF00397	0.245
DOC_WW_Pin1_4	182	187	PF00397	0.245
DOC_WW_Pin1_4	228	233	PF00397	0.245
DOC_WW_Pin1_4	55	60	PF00397	0.226
LIG_14-3-3_CanoR_1	125	131	PF00244	0.385
LIG_14-3-3_CanoR_1	3	8	PF00244	0.618
LIG_14-3-3_CanoR_1	343	349	PF00244	0.404
LIG_APCC_ABBA_1	192	197	PF00400	0.245
LIG_APCC_ABBAyCdc20_2	332	338	PF00400	0.223
LIG_BRCT_BRCA1_1	131	135	PF00533	0.245
LIG_BRCT_BRCA1_1	232	236	PF00533	0.245
LIG_BRCT_BRCA1_2	131	137	PF00533	0.245
LIG_CSL_BTD_1	183	186	PF09270	0.245
LIG_FHA_1	229	235	PF00498	0.245
LIG_FHA_1	324	330	PF00498	0.280
LIG_FHA_1	94	100	PF00498	0.446
LIG_FHA_2	156	162	PF00498	0.583
LIG_FHA_2	322	328	PF00498	0.377
LIG_LIR_Apic_2	112	118	PF02991	0.408
LIG_LIR_Apic_2	233	238	PF02991	0.498
LIG_LIR_Gen_1	103	113	PF02991	0.453
LIG_LIR_Gen_1	132	139	PF02991	0.254
LIG_LIR_Gen_1	40	46	PF02991	0.438
LIG_LIR_Gen_1	70	78	PF02991	0.386
LIG_LIR_Nem_3	103	108	PF02991	0.430
LIG_LIR_Nem_3	132	138	PF02991	0.374
LIG_LIR_Nem_3	276	282	PF02991	0.562
LIG_LIR_Nem_3	40	45	PF02991	0.438
LIG_LIR_Nem_3	70	74	PF02991	0.367
LIG_MLH1_MIPbox_1	232	236	PF16413	0.245
LIG_NRBOX	4	10	PF00104	0.539
LIG_Pex14_2	180	184	PF04695	0.353
LIG_Pex3_1	5	16	PF04882	0.595
LIG_SH2_CRK	133	137	PF00017	0.245
LIG_SH2_CRK	57	61	PF00017	0.245
LIG_SH2_PTP2	322	325	PF00017	0.277
LIG_SH2_STAP1	126	130	PF00017	0.469
LIG_SH2_STAT3	373	376	PF00017	0.535
LIG_SH2_STAT5	138	141	PF00017	0.528
LIG_SH2_STAT5	181	184	PF00017	0.380
LIG_SH2_STAT5	235	238	PF00017	0.473
LIG_SH2_STAT5	322	325	PF00017	0.339
LIG_SH2_STAT5	336	339	PF00017	0.339
LIG_SH2_STAT5	373	376	PF00017	0.535
LIG_SH2_STAT5	57	60	PF00017	0.226
LIG_SH2_STAT5	71	74	PF00017	0.365
LIG_SH3_3	105	111	PF00018	0.380
LIG_SH3_3	148	154	PF00018	0.646
LIG_SH3_3	180	186	PF00018	0.484
LIG_SH3_3	26	32	PF00018	0.560
LIG_SUMO_SIM_anti_2	6	12	PF11976	0.621
LIG_SUMO_SIM_anti_2	86	91	PF11976	0.471
LIG_SUMO_SIM_par_1	94	100	PF11976	0.245
LIG_TYR_ITIM	193	198	PF00017	0.414
LIG_TYR_ITIM	224	229	PF00017	0.245
LIG_TYR_ITIM	55	60	PF00017	0.245
LIG_UBA3_1	87	92	PF00899	0.456
MOD_CDK_SPxxK_3	182	189	PF00069	0.245
MOD_CK1_1	129	135	PF00069	0.330
MOD_CK1_1	216	222	PF00069	0.403
MOD_CK1_1	295	301	PF00069	0.478
MOD_CK2_1	155	161	PF00069	0.479
MOD_CK2_1	321	327	PF00069	0.421
MOD_GlcNHglycan	294	297	PF01048	0.429
MOD_GSK3_1	213	220	PF00069	0.424
MOD_N-GLC_1	25	30	PF02516	0.611
MOD_NEK2_1	344	349	PF00069	0.446
MOD_PK_1	3	9	PF00069	0.716
MOD_PKA_2	124	130	PF00069	0.385
MOD_Plk_1	25	31	PF00069	0.602
MOD_Plk_4	109	115	PF00069	0.367
MOD_Plk_4	131	137	PF00069	0.256
MOD_Plk_4	164	170	PF00069	0.414
MOD_Plk_4	230	236	PF00069	0.386
MOD_Plk_4	3	9	PF00069	0.605
MOD_ProDKin_1	107	113	PF00069	0.245
MOD_ProDKin_1	182	188	PF00069	0.245
MOD_ProDKin_1	228	234	PF00069	0.245
MOD_ProDKin_1	55	61	PF00069	0.226
TRG_DiLeu_BaEn_1	103	108	PF01217	0.245
TRG_DiLeu_BaEn_1	187	192	PF01217	0.245
TRG_ENDOCYTIC_2	133	136	PF00928	0.245
TRG_ENDOCYTIC_2	195	198	PF00928	0.401
TRG_ENDOCYTIC_2	226	229	PF00928	0.310
TRG_ENDOCYTIC_2	279	282	PF00928	0.426
TRG_ENDOCYTIC_2	322	325	PF00928	0.245
TRG_ENDOCYTIC_2	57	60	PF00928	0.469
TRG_ENDOCYTIC_2	71	74	PF00928	0.408
TRG_ER_diArg_1	248	251	PF00400	0.245
TRG_ER_diArg_1	62	65	PF00400	0.245

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVU7	Leptomonas seymouri	40%	100%
A0A0S4JJE1	Bodo saltans	32%	100%
A0A1X0NSF7	Trypanosomatidae	39%	99%
A0A3S7WZP2	Leishmania donovani	53%	100%
A0A422N2J8	Trypanosoma rangeli	36%	100%
A4I1Z8	Leishmania infantum	53%	100%
C9ZS16	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AY45	Leishmania mexicana (strain MHOM/GT/2001/U1103)	54%	100%
O13811	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
O22263	Arabidopsis thaliana	31%	100%
P38661	Medicago sativa	30%	100%
Q00216	Aspergillus niger	31%	100%
Q4Q9C8	Leishmania major	52%	100%
Q75M08	Oryza sativa subsp. japonica	31%	100%
Q86IA3	Dictyostelium discoideum	27%	100%
Q92249	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	100%
Q942L2	Oryza sativa subsp. japonica	30%	100%
Q9V438	Drosophila melanogaster	28%	88%
V5DR98	Trypanosoma cruzi	36%	100%