Pseudouridylate synthase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridylate synthase-like protein

Species:

Leishmania braziliensis

UniProt:

A4HEP8_LEIBR

TriTrypDb:

LbrM.26.0440 , LBRM2903_260009300

Length:

689

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

A4HEP8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEP8

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	6
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006396	RNA processing	6	6
GO:0006399	tRNA metabolic process	7	6
GO:0006400	tRNA modification	6	4
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008033	tRNA processing	8	6
GO:0008152	metabolic process	1	6
GO:0009451	RNA modification	5	6
GO:0009987	cellular process	1	6
GO:0016070	RNA metabolic process	5	6
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	4
GO:0034470	ncRNA processing	7	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0034660	ncRNA metabolic process	6	6
GO:0043170	macromolecule metabolic process	3	6
GO:0043412	macromolecule modification	4	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0046483	heterocycle metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:0090304	nucleic acid metabolic process	4	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004730	pseudouridylate synthase activity	5	8
GO:0005488	binding	1	11
GO:0009982	pseudouridine synthase activity	4	11
GO:0016829	lyase activity	2	8
GO:0016835	carbon-oxygen lyase activity	3	8
GO:0016836	hydro-lyase activity	4	8
GO:0016853	isomerase activity	2	11
GO:0016866	intramolecular transferase activity	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	585	589	PF00656	0.526
CLV_NRD_NRD_1	123	125	PF00675	0.309
CLV_NRD_NRD_1	13	15	PF00675	0.571
CLV_NRD_NRD_1	149	151	PF00675	0.327
CLV_NRD_NRD_1	246	248	PF00675	0.491
CLV_NRD_NRD_1	310	312	PF00675	0.386
CLV_NRD_NRD_1	380	382	PF00675	0.291
CLV_NRD_NRD_1	407	409	PF00675	0.387
CLV_NRD_NRD_1	561	563	PF00675	0.554
CLV_NRD_NRD_1	8	10	PF00675	0.592
CLV_PCSK_KEX2_1	123	125	PF00082	0.309
CLV_PCSK_KEX2_1	13	15	PF00082	0.567
CLV_PCSK_KEX2_1	149	151	PF00082	0.327
CLV_PCSK_KEX2_1	246	248	PF00082	0.519
CLV_PCSK_KEX2_1	407	409	PF00082	0.363
CLV_PCSK_KEX2_1	653	655	PF00082	0.553
CLV_PCSK_KEX2_1	7	9	PF00082	0.592
CLV_PCSK_PC1ET2_1	653	655	PF00082	0.568
CLV_PCSK_PC7_1	4	10	PF00082	0.702
CLV_PCSK_SKI1_1	105	109	PF00082	0.551
CLV_PCSK_SKI1_1	153	157	PF00082	0.406
CLV_PCSK_SKI1_1	286	290	PF00082	0.311
CLV_PCSK_SKI1_1	407	411	PF00082	0.413
CLV_PCSK_SKI1_1	515	519	PF00082	0.598
CLV_PCSK_SKI1_1	595	599	PF00082	0.533
CLV_PCSK_SKI1_1	666	670	PF00082	0.467
DEG_Nend_UBRbox_1	1	4	PF02207	0.746
DEG_SPOP_SBC_1	357	361	PF00917	0.369
DOC_CKS1_1	409	414	PF01111	0.476
DOC_CYCLIN_yCln2_LP_2	161	164	PF00134	0.411
DOC_MAPK_DCC_7	194	204	PF00069	0.486
DOC_MAPK_FxFP_2	288	291	PF00069	0.381
DOC_MAPK_gen_1	123	131	PF00069	0.416
DOC_MAPK_gen_1	149	158	PF00069	0.372
DOC_MAPK_gen_1	19	29	PF00069	0.363
DOC_MAPK_gen_1	311	318	PF00069	0.396
DOC_MAPK_gen_1	672	682	PF00069	0.507
DOC_MAPK_HePTP_8	277	289	PF00069	0.328
DOC_MAPK_MEF2A_6	22	31	PF00069	0.329
DOC_MAPK_MEF2A_6	280	289	PF00069	0.305
DOC_MAPK_MEF2A_6	96	103	PF00069	0.318
DOC_PP1_RVXF_1	284	290	PF00149	0.436
DOC_PP2B_LxvP_1	101	104	PF13499	0.358
DOC_PP2B_LxvP_1	161	164	PF13499	0.362
DOC_PP4_FxxP_1	288	291	PF00568	0.321
DOC_USP7_MATH_1	210	214	PF00917	0.703
DOC_USP7_MATH_1	218	222	PF00917	0.548
DOC_USP7_MATH_1	337	341	PF00917	0.567
DOC_USP7_MATH_1	394	398	PF00917	0.470
DOC_USP7_MATH_1	436	440	PF00917	0.440
DOC_USP7_MATH_1	525	529	PF00917	0.424
DOC_USP7_MATH_1	566	570	PF00917	0.640
DOC_WD40_RPTOR_TOS_1	251	256	PF00400	0.516
DOC_WW_Pin1_4	333	338	PF00397	0.573
DOC_WW_Pin1_4	348	353	PF00397	0.606
DOC_WW_Pin1_4	401	406	PF00397	0.413
DOC_WW_Pin1_4	408	413	PF00397	0.371
DOC_WW_Pin1_4	519	524	PF00397	0.398
DOC_WW_Pin1_4	573	578	PF00397	0.591
DOC_WW_Pin1_4	633	638	PF00397	0.626
LIG_14-3-3_CanoR_1	124	130	PF00244	0.432
LIG_14-3-3_CanoR_1	259	269	PF00244	0.583
LIG_14-3-3_CanoR_1	407	412	PF00244	0.416
LIG_14-3-3_CanoR_1	492	499	PF00244	0.400
LIG_14-3-3_CanoR_1	84	92	PF00244	0.350
LIG_14-3-3_CanoR_1	96	102	PF00244	0.300
LIG_Actin_WH2_2	49	64	PF00022	0.354
LIG_APCC_ABBA_1	304	309	PF00400	0.543
LIG_BIR_III_4	450	454	PF00653	0.431
LIG_BRCT_BRCA1_1	137	141	PF00533	0.402
LIG_CaM_IQ_9	179	195	PF13499	0.281
LIG_EH1_1	50	58	PF00400	0.265
LIG_EH1_1	92	100	PF00400	0.299
LIG_FHA_1	126	132	PF00498	0.383
LIG_FHA_1	323	329	PF00498	0.560
LIG_FHA_1	351	357	PF00498	0.610
LIG_FHA_1	358	364	PF00498	0.457
LIG_FHA_1	408	414	PF00498	0.456
LIG_FHA_1	529	535	PF00498	0.341
LIG_FHA_1	62	68	PF00498	0.291
LIG_FHA_2	499	505	PF00498	0.564
LIG_FHA_2	634	640	PF00498	0.593
LIG_LIR_Gen_1	166	175	PF02991	0.338
LIG_LIR_Gen_1	245	255	PF02991	0.368
LIG_LIR_Gen_1	531	539	PF02991	0.429
LIG_LIR_Gen_1	627	637	PF02991	0.516
LIG_LIR_Nem_3	106	111	PF02991	0.298
LIG_LIR_Nem_3	166	171	PF02991	0.319
LIG_LIR_Nem_3	245	251	PF02991	0.330
LIG_LIR_Nem_3	36	41	PF02991	0.332
LIG_LIR_Nem_3	424	429	PF02991	0.388
LIG_LIR_Nem_3	513	517	PF02991	0.540
LIG_LIR_Nem_3	531	536	PF02991	0.285
LIG_LIR_Nem_3	627	633	PF02991	0.550
LIG_PCNA_yPIPBox_3	144	155	PF02747	0.334
LIG_Pex14_2	648	652	PF04695	0.633
LIG_RPA_C_Fungi	254	266	PF08784	0.599
LIG_SH2_CRK	154	158	PF00017	0.304
LIG_SH2_CRK	159	163	PF00017	0.322
LIG_SH2_CRK	514	518	PF00017	0.545
LIG_SH2_GRB2like	248	251	PF00017	0.456
LIG_SH2_PTP2	201	204	PF00017	0.429
LIG_SH2_STAP1	314	318	PF00017	0.550
LIG_SH2_STAP1	429	433	PF00017	0.396
LIG_SH2_STAT3	420	423	PF00017	0.449
LIG_SH2_STAT5	181	184	PF00017	0.257
LIG_SH2_STAT5	201	204	PF00017	0.392
LIG_SH2_STAT5	418	421	PF00017	0.340
LIG_SH2_STAT5	632	635	PF00017	0.526
LIG_SH2_STAT5	647	650	PF00017	0.456
LIG_SH3_3	263	269	PF00018	0.450
LIG_SH3_3	406	412	PF00018	0.370
LIG_SH3_3	42	48	PF00018	0.393
LIG_SUMO_SIM_par_1	471	476	PF11976	0.362
LIG_SxIP_EBH_1	135	149	PF03271	0.399
LIG_TRAF2_1	597	600	PF00917	0.786
LIG_TRFH_1	159	163	PF08558	0.431
LIG_TYR_ITIM	199	204	PF00017	0.472
LIG_UBA3_1	376	382	PF00899	0.304
LIG_UBA3_1	57	62	PF00899	0.381
MOD_CDC14_SPxK_1	404	407	PF00782	0.403
MOD_CDK_SPxK_1	401	407	PF00069	0.390
MOD_CDK_SPxxK_3	401	408	PF00069	0.434
MOD_CK1_1	219	225	PF00069	0.518
MOD_CK1_1	240	246	PF00069	0.474
MOD_CK1_1	257	263	PF00069	0.476
MOD_CK1_1	340	346	PF00069	0.634
MOD_CK1_1	348	354	PF00069	0.577
MOD_CK1_1	528	534	PF00069	0.335
MOD_CK1_1	85	91	PF00069	0.356
MOD_CK2_1	205	211	PF00069	0.555
MOD_CK2_1	307	313	PF00069	0.529
MOD_CK2_1	394	400	PF00069	0.475
MOD_CK2_1	498	504	PF00069	0.531
MOD_CK2_1	618	624	PF00069	0.631
MOD_CK2_1	633	639	PF00069	0.592
MOD_DYRK1A_RPxSP_1	408	412	PF00069	0.478
MOD_GlcNHglycan	113	116	PF01048	0.399
MOD_GlcNHglycan	137	140	PF01048	0.566
MOD_GlcNHglycan	175	178	PF01048	0.391
MOD_GlcNHglycan	207	210	PF01048	0.394
MOD_GlcNHglycan	211	215	PF01048	0.537
MOD_GlcNHglycan	259	262	PF01048	0.600
MOD_GlcNHglycan	325	328	PF01048	0.757
MOD_GlcNHglycan	33	36	PF01048	0.447
MOD_GlcNHglycan	339	342	PF01048	0.678
MOD_GlcNHglycan	345	348	PF01048	0.579
MOD_GlcNHglycan	438	441	PF01048	0.491
MOD_GlcNHglycan	494	497	PF01048	0.494
MOD_GlcNHglycan	639	643	PF01048	0.490
MOD_GSK3_1	333	340	PF00069	0.581
MOD_GSK3_1	342	349	PF00069	0.541
MOD_GSK3_1	494	501	PF00069	0.597
MOD_GSK3_1	513	520	PF00069	0.564
MOD_GSK3_1	524	531	PF00069	0.510
MOD_GSK3_1	591	598	PF00069	0.607
MOD_GSK3_1	607	614	PF00069	0.748
MOD_N-GLC_1	249	254	PF02516	0.346
MOD_N-GLC_1	257	262	PF02516	0.496
MOD_N-GLC_1	436	441	PF02516	0.500
MOD_N-GLC_1	540	545	PF02516	0.325
MOD_N-GLC_1	589	594	PF02516	0.734
MOD_N-GLC_2	232	234	PF02516	0.266
MOD_NEK2_1	135	140	PF00069	0.480
MOD_NEK2_1	227	232	PF00069	0.364
MOD_NEK2_1	237	242	PF00069	0.358
MOD_NEK2_1	31	36	PF00069	0.321
MOD_NEK2_1	421	426	PF00069	0.316
MOD_NEK2_1	494	499	PF00069	0.604
MOD_NEK2_1	61	66	PF00069	0.276
MOD_NEK2_1	82	87	PF00069	0.374
MOD_PIKK_1	192	198	PF00454	0.554
MOD_PIKK_1	216	222	PF00454	0.634
MOD_PK_1	130	136	PF00069	0.251
MOD_PKA_1	407	413	PF00069	0.376
MOD_PKA_2	394	400	PF00069	0.345
MOD_PKA_2	407	413	PF00069	0.375
MOD_PKA_2	443	449	PF00069	0.395
MOD_PKB_1	490	498	PF00069	0.486
MOD_Plk_1	249	255	PF00069	0.377
MOD_Plk_1	429	435	PF00069	0.489
MOD_Plk_1	528	534	PF00069	0.335
MOD_Plk_1	540	546	PF00069	0.366
MOD_Plk_1	683	689	PF00069	0.514
MOD_Plk_4	130	136	PF00069	0.449
MOD_Plk_4	137	143	PF00069	0.472
MOD_Plk_4	219	225	PF00069	0.532
MOD_Plk_4	358	364	PF00069	0.438
MOD_Plk_4	371	377	PF00069	0.358
MOD_Plk_4	383	389	PF00069	0.361
MOD_Plk_4	394	400	PF00069	0.348
MOD_Plk_4	498	504	PF00069	0.626
MOD_Plk_4	528	534	PF00069	0.380
MOD_Plk_4	97	103	PF00069	0.432
MOD_ProDKin_1	333	339	PF00069	0.573
MOD_ProDKin_1	348	354	PF00069	0.596
MOD_ProDKin_1	401	407	PF00069	0.418
MOD_ProDKin_1	408	414	PF00069	0.367
MOD_ProDKin_1	519	525	PF00069	0.396
MOD_ProDKin_1	573	579	PF00069	0.598
MOD_ProDKin_1	633	639	PF00069	0.622
MOD_SUMO_rev_2	550	556	PF00179	0.326
MOD_SUMO_rev_2	585	594	PF00179	0.617
TRG_DiLeu_BaEn_1	301	306	PF01217	0.460
TRG_DiLeu_BaEn_1	504	509	PF01217	0.395
TRG_DiLeu_BaEn_2	370	376	PF01217	0.308
TRG_DiLeu_BaEn_2	528	534	PF01217	0.314
TRG_DiLeu_BaLyEn_6	532	537	PF01217	0.421
TRG_ENDOCYTIC_2	152	155	PF00928	0.288
TRG_ENDOCYTIC_2	159	162	PF00928	0.282
TRG_ENDOCYTIC_2	201	204	PF00928	0.392
TRG_ENDOCYTIC_2	248	251	PF00928	0.456
TRG_ENDOCYTIC_2	418	421	PF00928	0.351
TRG_ENDOCYTIC_2	514	517	PF00928	0.542
TRG_ER_diArg_1	12	14	PF00400	0.591
TRG_ER_diArg_1	123	125	PF00400	0.309
TRG_ER_diArg_1	149	151	PF00400	0.327
TRG_ER_diArg_1	246	248	PF00400	0.531
TRG_ER_diArg_1	407	409	PF00400	0.387
TRG_ER_diArg_1	559	562	PF00400	0.513
TRG_ER_diArg_1	654	657	PF00400	0.544
TRG_ER_diArg_1	7	9	PF00400	0.624
TRG_ER_diArg_1	71	74	PF00400	0.429
TRG_NLS_MonoExtC_3	652	657	PF00514	0.607
TRG_NLS_MonoExtN_4	650	657	PF00514	0.482
TRG_Pf-PMV_PEXEL_1	39	44	PF00026	0.357
TRG_Pf-PMV_PEXEL_1	554	558	PF00026	0.530

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5J6	Leptomonas seymouri	59%	100%
A0A0S4KL20	Bodo saltans	34%	93%
A0A1X0NSY0	Trypanosomatidae	40%	100%
A0A3Q8ICM8	Leishmania donovani	79%	99%
A0A3R7KTM1	Trypanosoma rangeli	40%	100%
A4I1X8	Leishmania infantum	79%	99%
C9ZS41	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AY24	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	99%
Q4Q9E9	Leishmania major	80%	100%