6-phosphofructo-2-kinase/fructose-2,6-biphosphata se-1-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein

Species:

Leishmania braziliensis

UniProt:

A4HEN8_LEIBR

TriTrypDb:

LbrM.26.0340 , LBRM2903_260008200

Length:

667

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A4HEN8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEN8

Function

Biological processes
Term	Name	Level	Count
GO:0005975	carbohydrate metabolic process	3	12
GO:0005996	monosaccharide metabolic process	3	12
GO:0006000	fructose metabolic process	5	12
GO:0006003	fructose 2,6-bisphosphate metabolic process	4	12
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0019318	hexose metabolic process	4	12
GO:0019637	organophosphate metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901135	carbohydrate derivative metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0003873	6-phosphofructo-2-kinase activity	6	12
GO:0004331	fructose-2,6-bisphosphate 2-phosphatase activity	8	7
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008443	phosphofructokinase activity	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0016787	hydrolase activity	2	7
GO:0016788	hydrolase activity, acting on ester bonds	3	7
GO:0016791	phosphatase activity	5	7
GO:0017076	purine nucleotide binding	4	12
GO:0019200	carbohydrate kinase activity	5	12
GO:0019203	carbohydrate phosphatase activity	6	7
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0042578	phosphoric ester hydrolase activity	4	7
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0050308	sugar-phosphatase activity	7	7
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	613	617	PF00656	0.505
CLV_NRD_NRD_1	240	242	PF00675	0.376
CLV_NRD_NRD_1	260	262	PF00675	0.122
CLV_NRD_NRD_1	28	30	PF00675	0.600
CLV_NRD_NRD_1	31	33	PF00675	0.578
CLV_NRD_NRD_1	319	321	PF00675	0.244
CLV_PCSK_FUR_1	29	33	PF00082	0.589
CLV_PCSK_KEX2_1	240	242	PF00082	0.337
CLV_PCSK_KEX2_1	259	261	PF00082	0.168
CLV_PCSK_KEX2_1	30	32	PF00082	0.630
CLV_PCSK_KEX2_1	319	321	PF00082	0.244
CLV_PCSK_KEX2_1	488	490	PF00082	0.412
CLV_PCSK_PC1ET2_1	30	32	PF00082	0.665
CLV_PCSK_PC1ET2_1	488	490	PF00082	0.400
CLV_PCSK_SKI1_1	107	111	PF00082	0.243
CLV_PCSK_SKI1_1	264	268	PF00082	0.389
CLV_PCSK_SKI1_1	288	292	PF00082	0.231
CLV_PCSK_SKI1_1	337	341	PF00082	0.339
CLV_PCSK_SKI1_1	353	357	PF00082	0.243
CLV_PCSK_SKI1_1	359	363	PF00082	0.363
CLV_PCSK_SKI1_1	418	422	PF00082	0.388
CLV_PCSK_SKI1_1	464	468	PF00082	0.305
DEG_SPOP_SBC_1	475	479	PF00917	0.166
DOC_CYCLIN_RxL_1	458	468	PF00134	0.388
DOC_CYCLIN_RxL_1	521	532	PF00134	0.286
DOC_CYCLIN_yClb1_LxF_4	416	421	PF00134	0.388
DOC_MAPK_gen_1	29	38	PF00069	0.649
DOC_MAPK_gen_1	458	467	PF00069	0.166
DOC_MAPK_MEF2A_6	135	143	PF00069	0.308
DOC_PP1_RVXF_1	32	39	PF00149	0.627
DOC_PP1_RVXF_1	335	341	PF00149	0.243
DOC_PP1_RVXF_1	416	422	PF00149	0.388
DOC_PP1_RVXF_1	462	468	PF00149	0.166
DOC_PP1_RVXF_1	522	529	PF00149	0.308
DOC_USP7_MATH_1	147	151	PF00917	0.376
DOC_USP7_MATH_1	16	20	PF00917	0.678
DOC_USP7_MATH_1	498	502	PF00917	0.350
DOC_USP7_UBL2_3	30	34	PF12436	0.657
DOC_WW_Pin1_4	11	16	PF00397	0.663
DOC_WW_Pin1_4	155	160	PF00397	0.258
DOC_WW_Pin1_4	197	202	PF00397	0.573
DOC_WW_Pin1_4	275	280	PF00397	0.346
DOC_WW_Pin1_4	426	431	PF00397	0.331
DOC_WW_Pin1_4	504	509	PF00397	0.166
DOC_WW_Pin1_4	515	520	PF00397	0.247
DOC_WW_Pin1_4	590	595	PF00397	0.363
DOC_WW_Pin1_4	74	79	PF00397	0.741
LIG_14-3-3_CanoR_1	230	235	PF00244	0.277
LIG_14-3-3_CanoR_1	454	462	PF00244	0.337
LIG_14-3-3_CanoR_1	489	495	PF00244	0.255
LIG_BIR_II_1	1	5	PF00653	0.662
LIG_BIR_III_4	102	106	PF00653	0.438
LIG_BRCT_BRCA1_1	175	179	PF00533	0.516
LIG_Dynein_DLC8_1	487	493	PF01221	0.337
LIG_eIF4E_1	121	127	PF01652	0.388
LIG_FHA_1	265	271	PF00498	0.426
LIG_FHA_1	296	302	PF00498	0.264
LIG_FHA_1	328	334	PF00498	0.246
LIG_FHA_1	403	409	PF00498	0.267
LIG_FHA_1	475	481	PF00498	0.329
LIG_FHA_1	489	495	PF00498	0.185
LIG_FHA_1	5	11	PF00498	0.640
LIG_FHA_1	590	596	PF00498	0.314
LIG_FHA_1	89	95	PF00498	0.428
LIG_FHA_2	156	162	PF00498	0.288
LIG_FHA_2	263	269	PF00498	0.365
LIG_FHA_2	389	395	PF00498	0.284
LIG_FHA_2	508	514	PF00498	0.419
LIG_FHA_2	528	534	PF00498	0.277
LIG_FHA_2	611	617	PF00498	0.501
LIG_FHA_2	75	81	PF00498	0.615
LIG_GBD_Chelix_1	289	297	PF00786	0.217
LIG_Integrin_isoDGR_2	133	135	PF01839	0.308
LIG_LIR_Gen_1	208	217	PF02991	0.460
LIG_LIR_Gen_1	37	44	PF02991	0.476
LIG_LIR_Gen_1	394	402	PF02991	0.243
LIG_LIR_Gen_1	440	449	PF02991	0.246
LIG_LIR_Nem_3	165	171	PF02991	0.450
LIG_LIR_Nem_3	19	25	PF02991	0.734
LIG_LIR_Nem_3	208	212	PF02991	0.501
LIG_LIR_Nem_3	371	376	PF02991	0.258
LIG_LIR_Nem_3	381	387	PF02991	0.221
LIG_LIR_Nem_3	394	400	PF02991	0.236
LIG_LIR_Nem_3	440	444	PF02991	0.254
LIG_PCNA_yPIPBox_3	325	339	PF02747	0.166
LIG_SH2_CRK	384	388	PF00017	0.258
LIG_SH2_CRK	462	466	PF00017	0.166
LIG_SH2_PTP2	397	400	PF00017	0.258
LIG_SH2_PTP2	441	444	PF00017	0.302
LIG_SH2_STAP1	406	410	PF00017	0.388
LIG_SH2_STAP1	462	466	PF00017	0.258
LIG_SH2_STAP1	608	612	PF00017	0.258
LIG_SH2_STAT5	234	237	PF00017	0.255
LIG_SH2_STAT5	242	245	PF00017	0.228
LIG_SH2_STAT5	300	303	PF00017	0.337
LIG_SH2_STAT5	322	325	PF00017	0.308
LIG_SH2_STAT5	397	400	PF00017	0.270
LIG_SH2_STAT5	434	437	PF00017	0.295
LIG_SH2_STAT5	441	444	PF00017	0.298
LIG_SH2_STAT5	469	472	PF00017	0.258
LIG_SH2_STAT5	496	499	PF00017	0.302
LIG_SH2_STAT5	527	530	PF00017	0.243
LIG_SH2_STAT5	564	567	PF00017	0.325
LIG_SH2_STAT5	610	613	PF00017	0.267
LIG_SH3_3	209	215	PF00018	0.536
LIG_SH3_3	222	228	PF00018	0.182
LIG_SH3_3	273	279	PF00018	0.451
LIG_SH3_3	360	366	PF00018	0.337
LIG_SH3_3	513	519	PF00018	0.256
LIG_SH3_3	78	84	PF00018	0.580
LIG_SUMO_SIM_anti_2	219	225	PF11976	0.258
LIG_SUMO_SIM_anti_2	304	311	PF11976	0.262
LIG_SUMO_SIM_anti_2	440	447	PF11976	0.302
LIG_SUMO_SIM_anti_2	476	485	PF11976	0.391
LIG_SUMO_SIM_par_1	139	144	PF11976	0.466
LIG_SUMO_SIM_par_1	158	165	PF11976	0.170
LIG_SUMO_SIM_par_1	591	599	PF11976	0.245
LIG_SUMO_SIM_par_1	647	653	PF11976	0.318
LIG_SUMO_SIM_par_1	88	93	PF11976	0.362
LIG_TYR_ITAM	381	400	PF00017	0.276
LIG_UBA3_1	223	232	PF00899	0.243
MOD_CDK_SPxxK_3	515	522	PF00069	0.166
MOD_CK1_1	2	8	PF00069	0.544
MOD_CK1_1	200	206	PF00069	0.554
MOD_CK1_1	275	281	PF00069	0.343
MOD_CK1_1	453	459	PF00069	0.243
MOD_CK1_1	476	482	PF00069	0.291
MOD_CK1_1	506	512	PF00069	0.205
MOD_CK2_1	155	161	PF00069	0.350
MOD_CK2_1	192	198	PF00069	0.486
MOD_CK2_1	20	26	PF00069	0.678
MOD_CK2_1	262	268	PF00069	0.267
MOD_CK2_1	507	513	PF00069	0.381
MOD_CK2_1	576	582	PF00069	0.393
MOD_CK2_1	658	664	PF00069	0.411
MOD_GlcNHglycan	11	14	PF01048	0.739
MOD_GlcNHglycan	208	212	PF01048	0.604
MOD_GlcNHglycan	22	25	PF01048	0.613
MOD_GlcNHglycan	304	307	PF01048	0.320
MOD_GlcNHglycan	411	414	PF01048	0.167
MOD_GlcNHglycan	99	102	PF01048	0.376
MOD_GSK3_1	16	23	PF00069	0.476
MOD_GSK3_1	188	195	PF00069	0.611
MOD_GSK3_1	400	407	PF00069	0.409
MOD_GSK3_1	474	481	PF00069	0.294
MOD_GSK3_1	5	12	PF00069	0.538
MOD_GSK3_1	503	510	PF00069	0.235
MOD_GSK3_1	547	554	PF00069	0.267
MOD_GSK3_1	58	65	PF00069	0.413
MOD_GSK3_1	606	613	PF00069	0.306
MOD_GSK3_1	660	667	PF00069	0.468
MOD_N-GLC_1	333	338	PF02516	0.365
MOD_N-GLC_1	424	429	PF02516	0.367
MOD_N-GLC_1	62	67	PF02516	0.551
MOD_N-GLC_1	74	79	PF02516	0.679
MOD_NEK2_1	216	221	PF00069	0.266
MOD_NEK2_1	310	315	PF00069	0.256
MOD_NEK2_1	433	438	PF00069	0.277
MOD_NEK2_1	596	601	PF00069	0.243
MOD_NEK2_2	490	495	PF00069	0.277
MOD_NEK2_2	498	503	PF00069	0.277
MOD_PIKK_1	16	22	PF00454	0.461
MOD_PIKK_1	310	316	PF00454	0.348
MOD_PIKK_1	400	406	PF00454	0.308
MOD_PIKK_1	424	430	PF00454	0.273
MOD_PIKK_1	488	494	PF00454	0.323
MOD_PK_1	272	278	PF00069	0.166
MOD_PKA_1	488	494	PF00069	0.388
MOD_PKA_2	20	26	PF00069	0.472
MOD_PKA_2	327	333	PF00069	0.305
MOD_PKA_2	453	459	PF00069	0.277
MOD_PKA_2	488	494	PF00069	0.255
MOD_PKB_1	458	466	PF00069	0.308
MOD_Plk_1	2	8	PF00069	0.457
MOD_Plk_1	207	213	PF00069	0.508
MOD_Plk_1	262	268	PF00069	0.166
MOD_Plk_1	272	278	PF00069	0.144
MOD_Plk_1	295	301	PF00069	0.277
MOD_Plk_1	333	339	PF00069	0.388
MOD_Plk_1	393	399	PF00069	0.161
MOD_Plk_1	498	504	PF00069	0.277
MOD_Plk_4	122	128	PF00069	0.343
MOD_Plk_4	147	153	PF00069	0.284
MOD_Plk_4	216	222	PF00069	0.246
MOD_Plk_4	230	236	PF00069	0.255
MOD_Plk_4	272	278	PF00069	0.450
MOD_Plk_4	296	302	PF00069	0.420
MOD_Plk_4	393	399	PF00069	0.174
MOD_Plk_4	478	484	PF00069	0.337
MOD_Plk_4	606	612	PF00069	0.315
MOD_ProDKin_1	11	17	PF00069	0.664
MOD_ProDKin_1	155	161	PF00069	0.258
MOD_ProDKin_1	197	203	PF00069	0.569
MOD_ProDKin_1	275	281	PF00069	0.346
MOD_ProDKin_1	426	432	PF00069	0.331
MOD_ProDKin_1	504	510	PF00069	0.166
MOD_ProDKin_1	515	521	PF00069	0.247
MOD_ProDKin_1	590	596	PF00069	0.363
MOD_ProDKin_1	74	80	PF00069	0.735
MOD_SUMO_rev_2	56	66	PF00179	0.612
TRG_DiLeu_BaEn_1	440	445	PF01217	0.277
TRG_ENDOCYTIC_2	22	25	PF00928	0.704
TRG_ENDOCYTIC_2	373	376	PF00928	0.254
TRG_ENDOCYTIC_2	384	387	PF00928	0.224
TRG_ENDOCYTIC_2	397	400	PF00928	0.243
TRG_ENDOCYTIC_2	441	444	PF00928	0.252
TRG_ENDOCYTIC_2	462	465	PF00928	0.166
TRG_ENDOCYTIC_2	608	611	PF00928	0.307
TRG_ER_diArg_1	239	241	PF00400	0.385
TRG_ER_diArg_1	259	261	PF00400	0.122
TRG_ER_diArg_1	29	32	PF00400	0.594
TRG_ER_diArg_1	521	524	PF00400	0.418
TRG_NLS_MonoCore_2	28	33	PF00514	0.568
TRG_NLS_MonoExtC_3	29	34	PF00514	0.658
TRG_NLS_MonoExtN_4	29	34	PF00514	0.612
TRG_Pf-PMV_PEXEL_1	325	329	PF00026	0.388
TRG_Pf-PMV_PEXEL_1	464	468	PF00026	0.337

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXF4	Leptomonas seymouri	72%	98%
A0A0S4IN06	Bodo saltans	40%	90%
A0A0S4KH03	Bodo saltans	24%	100%
A0A1X0NU02	Trypanosomatidae	53%	100%
A0A3R7MQ36	Trypanosoma rangeli	55%	100%
A0A3S7WZP0	Leishmania donovani	86%	100%
A4I1W7	Leishmania infantum	86%	100%
C9ZS53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
C9ZUM0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	95%
E9AY13	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
O35552	Rattus norvegicus	27%	100%
O60825	Homo sapiens	27%	100%
P07953	Rattus norvegicus	29%	100%
P16118	Homo sapiens	28%	100%
P25114	Rattus norvegicus	28%	100%
P26285	Bos taurus	27%	100%
P49872	Bos taurus	28%	100%
P70265	Mus musculus	27%	100%
P70266	Mus musculus	29%	100%
Q06137	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q16875	Homo sapiens	27%	100%
Q16877	Homo sapiens	28%	100%
Q28901	Bos taurus	27%	100%
Q4Q9G0	Leishmania major	85%	100%
Q4R8B6	Macaca fascicularis	28%	100%
Q5NVT1	Pongo abelii	27%	100%
Q5R9C1	Pongo abelii	27%	100%
Q6DTY7	Mus musculus	28%	100%
Q8TFH0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
Q91309	Lithobates catesbeianus	27%	100%
Q91348	Gallus gallus	28%	100%
Q9JJH5	Rattus norvegicus	27%	100%
Q9MB58	Arabidopsis thaliana	31%	90%
V5DR58	Trypanosoma cruzi	56%	97%