Hydrolase_4 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Uncharacterised conserved protein (DUF2305), putative

Species:

Leishmania braziliensis

UniProt:

A4HEN1_LEIBR

TriTrypDb:

LbrM.26.0250 , LBRM2903_260007300

Length:

314

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005811	lipid droplet	5	12
GO:0043226	organelle	2	12
GO:0043228	non-membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043232	intracellular non-membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0016020	membrane	2	7

Expansion

Sequence features

A4HEN1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEN1

Function

Biological processes
Term	Name	Level	Count
GO:0019915	lipid storage	3	12
GO:0051179	localization	1	12
GO:0051235	maintenance of location	2	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016298	lipase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0016788	hydrolase activity, acting on ester bonds	3	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	106	110	PF00656	0.412
CLV_NRD_NRD_1	170	172	PF00675	0.366
CLV_NRD_NRD_1	49	51	PF00675	0.282
CLV_PCSK_KEX2_1	170	172	PF00082	0.360
CLV_PCSK_KEX2_1	276	278	PF00082	0.376
CLV_PCSK_PC1ET2_1	276	278	PF00082	0.376
CLV_PCSK_SKI1_1	170	174	PF00082	0.268
CLV_PCSK_SKI1_1	199	203	PF00082	0.510
CLV_PCSK_SKI1_1	239	243	PF00082	0.491
CLV_PCSK_SKI1_1	255	259	PF00082	0.320
CLV_PCSK_SKI1_1	50	54	PF00082	0.251
DEG_APCC_DBOX_1	254	262	PF00400	0.356
DOC_MAPK_gen_1	123	130	PF00069	0.475
DOC_MAPK_gen_1	50	56	PF00069	0.431
DOC_MAPK_gen_1	75	82	PF00069	0.409
DOC_PP1_RVXF_1	49	56	PF00149	0.553
DOC_PP4_FxxP_1	65	68	PF00568	0.518
DOC_USP7_MATH_1	86	90	PF00917	0.470
DOC_WW_Pin1_4	163	168	PF00397	0.446
DOC_WW_Pin1_4	208	213	PF00397	0.211
LIG_14-3-3_CanoR_1	97	102	PF00244	0.530
LIG_Actin_WH2_2	234	252	PF00022	0.285
LIG_BIR_II_1	1	5	PF00653	0.602
LIG_eIF4E_1	196	202	PF01652	0.319
LIG_FHA_2	32	38	PF00498	0.473
LIG_Integrin_isoDGR_2	168	170	PF01839	0.334
LIG_LIR_Gen_1	230	240	PF02991	0.328
LIG_LIR_Nem_3	236	241	PF02991	0.250
LIG_LIR_Nem_3	264	269	PF02991	0.312
LIG_MYND_1	208	212	PF01753	0.272
LIG_NRBOX	197	203	PF00104	0.351
LIG_PTB_Apo_2	226	233	PF02174	0.312
LIG_PTB_Phospho_1	226	232	PF10480	0.337
LIG_SH2_CRK	147	151	PF00017	0.556
LIG_SH2_STAP1	271	275	PF00017	0.272
LIG_SH2_STAT3	259	262	PF00017	0.272
LIG_SH2_STAT5	129	132	PF00017	0.387
LIG_SH2_STAT5	174	177	PF00017	0.399
LIG_SH2_STAT5	260	263	PF00017	0.264
LIG_SH2_STAT5	285	288	PF00017	0.176
LIG_SH2_STAT5	32	35	PF00017	0.539
LIG_SH3_3	51	57	PF00018	0.464
LIG_SUMO_SIM_anti_2	79	84	PF11976	0.458
LIG_TRFH_1	154	158	PF08558	0.512
LIG_UBA3_1	190	197	PF00899	0.366
MOD_CDK_SPxxK_3	163	170	PF00069	0.485
MOD_CK1_1	3	9	PF00069	0.612
MOD_CK2_1	267	273	PF00069	0.368
MOD_CK2_1	31	37	PF00069	0.597
MOD_CK2_1	97	103	PF00069	0.529
MOD_Cter_Amidation	168	171	PF01082	0.306
MOD_GSK3_1	107	114	PF00069	0.517
MOD_GSK3_1	173	180	PF00069	0.464
MOD_GSK3_1	31	38	PF00069	0.634
MOD_NEK2_1	111	116	PF00069	0.476
MOD_NEK2_1	173	178	PF00069	0.485
MOD_NEK2_1	227	232	PF00069	0.320
MOD_NEK2_1	23	28	PF00069	0.576
MOD_PIKK_1	177	183	PF00454	0.198
MOD_PIKK_1	88	94	PF00454	0.376
MOD_PK_1	146	152	PF00069	0.472
MOD_Plk_4	107	113	PF00069	0.508
MOD_Plk_4	227	233	PF00069	0.345
MOD_Plk_4	3	9	PF00069	0.601
MOD_Plk_4	301	307	PF00069	0.286
MOD_ProDKin_1	163	169	PF00069	0.446
MOD_ProDKin_1	208	214	PF00069	0.211
MOD_SUMO_rev_2	270	278	PF00179	0.230
TRG_DiLeu_BaEn_1	237	242	PF01217	0.337
TRG_DiLeu_BaLyEn_6	252	257	PF01217	0.328
TRG_ENDOCYTIC_2	147	150	PF00928	0.537
TRG_ENDOCYTIC_2	232	235	PF00928	0.351
TRG_ENDOCYTIC_2	66	69	PF00928	0.451
TRG_ER_diArg_1	170	172	PF00400	0.529
TRG_NES_CRM1_1	192	207	PF08389	0.315
TRG_Pf-PMV_PEXEL_1	239	243	PF00026	0.537

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJP9	Leptomonas seymouri	63%	100%
A0A0S4IN66	Bodo saltans	37%	100%
A0A1X0NSK1	Trypanosomatidae	46%	100%
A0A3S7WZH7	Leishmania donovani	80%	100%
A0A422NVG9	Trypanosoma rangeli	42%	100%
A4I1V9	Leishmania infantum	81%	100%
C9ZS60	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	98%
E9AY04	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
Q4Q9G9	Leishmania major	82%	100%
Q5F477	Gallus gallus	25%	97%
Q5HZX7	Rattus norvegicus	26%	97%
Q5R7E8	Pongo abelii	28%	97%
Q8BVA5	Mus musculus	25%	96%
Q9H6V9	Homo sapiens	28%	97%
Q9W0H3	Drosophila melanogaster	25%	100%
V5BUL9	Trypanosoma cruzi	43%	100%