Nfu_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Scaffold protein Nfu/NifU N terminal/NifU-like domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HEM5_LEIBR

TriTrypDb:

LbrM.26.0190 , LBRM2903_260007000

Length:

282

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HEM5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEM5

Function

Biological processes
Term	Name	Level	Count
GO:0006790	sulfur compound metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0016226	iron-sulfur cluster assembly	4	11
GO:0022607	cellular component assembly	4	11
GO:0031163	metallo-sulfur cluster assembly	5	11
GO:0044237	cellular metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0006807	nitrogen compound metabolic process	2	1
GO:0019538	protein metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044238	primary metabolic process	2	1
GO:0051604	protein maturation	4	1
GO:0071704	organic substance metabolic process	2	1
GO:0097428	protein maturation by iron-sulfur cluster transfer	5	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005506	iron ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0051536	iron-sulfur cluster binding	3	11
GO:0051540	metal cluster binding	2	11
GO:0051539	4 iron, 4 sulfur cluster binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	128	130	PF00675	0.289
CLV_NRD_NRD_1	268	270	PF00675	0.445
CLV_NRD_NRD_1	3	5	PF00675	0.614
CLV_NRD_NRD_1	34	36	PF00675	0.489
CLV_PCSK_KEX2_1	266	268	PF00082	0.413
CLV_PCSK_KEX2_1	3	5	PF00082	0.596
CLV_PCSK_KEX2_1	34	36	PF00082	0.489
CLV_PCSK_PC1ET2_1	266	268	PF00082	0.498
CLV_PCSK_SKI1_1	187	191	PF00082	0.214
CLV_PCSK_SKI1_1	195	199	PF00082	0.236
CLV_PCSK_SKI1_1	270	274	PF00082	0.532
DEG_APCC_KENBOX_2	153	157	PF00400	0.442
DEG_Nend_Nbox_1	1	3	PF02207	0.467
DOC_CYCLIN_RxL_1	184	192	PF00134	0.244
DOC_MAPK_gen_1	15	23	PF00069	0.612
DOC_MAPK_gen_1	3	11	PF00069	0.521
DOC_MAPK_RevD_3	114	130	PF00069	0.323
DOC_PP4_FxxP_1	86	89	PF00568	0.273
DOC_USP7_MATH_1	222	226	PF00917	0.366
DOC_USP7_MATH_1	28	32	PF00917	0.645
DOC_USP7_MATH_1	50	54	PF00917	0.474
DOC_USP7_UBL2_3	266	270	PF12436	0.478
DOC_WW_Pin1_4	101	106	PF00397	0.219
DOC_WW_Pin1_4	68	73	PF00397	0.264
DOC_WW_Pin1_4	94	99	PF00397	0.282
LIG_14-3-3_CanoR_1	29	37	PF00244	0.517
LIG_14-3-3_CanoR_1	78	83	PF00244	0.260
LIG_BRCT_BRCA1_1	80	84	PF00533	0.267
LIG_CORNRBOX	139	147	PF00104	0.163
LIG_FHA_1	18	24	PF00498	0.560
LIG_FHA_1	212	218	PF00498	0.422
LIG_LIR_Apic_2	83	89	PF02991	0.244
LIG_LIR_Gen_1	123	132	PF02991	0.253
LIG_LIR_Gen_1	214	222	PF02991	0.421
LIG_LIR_Gen_1	81	91	PF02991	0.163
LIG_LIR_LC3C_4	63	66	PF02991	0.262
LIG_LIR_Nem_3	123	128	PF02991	0.244
LIG_LIR_Nem_3	135	139	PF02991	0.244
LIG_LIR_Nem_3	214	219	PF02991	0.443
LIG_LIR_Nem_3	81	87	PF02991	0.163
LIG_Pex14_2	80	84	PF04695	0.327
LIG_SH2_CRK	102	106	PF00017	0.263
LIG_SH2_PTP2	125	128	PF00017	0.244
LIG_SH2_STAT5	125	128	PF00017	0.258
LIG_SUMO_SIM_par_1	177	188	PF11976	0.365
LIG_SUMO_SIM_par_1	19	25	PF11976	0.430
LIG_SUMO_SIM_par_1	247	253	PF11976	0.362
MOD_CK1_1	225	231	PF00069	0.404
MOD_CK1_1	44	50	PF00069	0.574
MOD_CK2_1	232	238	PF00069	0.501
MOD_GlcNHglycan	11	14	PF01048	0.519
MOD_GlcNHglycan	234	237	PF01048	0.231
MOD_GlcNHglycan	30	33	PF01048	0.495
MOD_GlcNHglycan	43	46	PF01048	0.504
MOD_GSK3_1	17	24	PF00069	0.510
MOD_GSK3_1	222	229	PF00069	0.409
MOD_GSK3_1	33	40	PF00069	0.646
MOD_GSK3_1	46	53	PF00069	0.526
MOD_GSK3_1	78	85	PF00069	0.244
MOD_N-GLC_1	68	73	PF02516	0.323
MOD_NEK2_1	17	22	PF00069	0.461
MOD_NEK2_1	189	194	PF00069	0.446
MOD_NEK2_1	37	42	PF00069	0.541
MOD_NEK2_1	82	87	PF00069	0.249
MOD_NEK2_1	9	14	PF00069	0.510
MOD_PKA_2	17	23	PF00069	0.614
MOD_PKA_2	28	34	PF00069	0.576
MOD_Plk_1	82	88	PF00069	0.244
MOD_Plk_1	89	95	PF00069	0.244
MOD_Plk_4	17	23	PF00069	0.479
MOD_Plk_4	211	217	PF00069	0.424
MOD_Plk_4	37	43	PF00069	0.632
MOD_ProDKin_1	101	107	PF00069	0.219
MOD_ProDKin_1	68	74	PF00069	0.264
MOD_ProDKin_1	94	100	PF00069	0.282
MOD_SUMO_for_1	87	90	PF00179	0.376
TRG_DiLeu_BaEn_2	211	217	PF01217	0.462
TRG_ENDOCYTIC_2	125	128	PF00928	0.244
TRG_ER_diArg_1	193	196	PF00400	0.407
TRG_ER_diArg_1	2	4	PF00400	0.624
TRG_ER_diArg_1	33	35	PF00400	0.496
TRG_Pf-PMV_PEXEL_1	62	67	PF00026	0.373

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2Q0	Leptomonas seymouri	79%	97%
A0A0N1I735	Leptomonas seymouri	34%	100%
A0A0S4IJH0	Bodo saltans	62%	100%
A0A0S4IVB5	Bodo saltans	37%	100%
A0A1X0NST9	Trypanosomatidae	61%	99%
A0A1X0P8F8	Trypanosomatidae	32%	100%
A0A3R7MX73	Trypanosoma rangeli	61%	100%
A0A3S5H7F5	Leishmania donovani	81%	99%
A0A3S7XAI2	Leishmania donovani	34%	94%
A4HNU8	Leishmania braziliensis	34%	100%
A4I1V6	Leishmania infantum	81%	99%
A4ICP6	Leishmania infantum	34%	94%
B3MRT7	Drosophila ananassae	36%	99%
B3NYF7	Drosophila erecta	37%	100%
B4H303	Drosophila persimilis	41%	100%
B4IMF6	Drosophila sechellia	38%	100%
B4JWR9	Drosophila grimshawi	42%	95%
B4M375	Drosophila virilis	41%	95%
B4NE93	Drosophila willistoni	38%	98%
B4PZ52	Drosophila yakuba	41%	100%
B4R3T1	Drosophila simulans	38%	100%
B5DKJ8	Drosophila pseudoobscura pseudoobscura	38%	99%
C9ZS65	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
D0A2R6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9ASK8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	94%
E9AY01	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
P32860	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	100%
Q4Q1Y8	Leishmania major	34%	91%
Q4Q9H2	Leishmania major	90%	100%
Q8SY96	Drosophila melanogaster	39%	100%
Q9C8J2	Arabidopsis thaliana	41%	100%
Q9LIG6	Arabidopsis thaliana	39%	100%
Q9QZ23	Mus musculus	41%	100%
Q9UMS0	Homo sapiens	41%	100%
Q9UUB8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	100%
V5DRN0	Trypanosoma cruzi	35%	100%