Diacylglycerol acyltransferase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diacylglycerol acyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4HEI1_LEIBR

TriTrypDb:

LbrM.25.2380 , LBRM2903_250025100

Length:

608

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	8
GO:0110165	cellular anatomical entity	1	8
GO:0005737	cytoplasm	2	1
GO:0005789	endoplasmic reticulum membrane	4	3
GO:0031090	organelle membrane	3	3
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

A4HEI1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEI1

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	1
GO:0006638	neutral lipid metabolic process	4	1
GO:0006639	acylglycerol metabolic process	5	1
GO:0006641	triglyceride metabolic process	6	1
GO:0006643	membrane lipid metabolic process	4	1
GO:0008152	metabolic process	1	1
GO:0008610	lipid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	1
GO:0019432	triglyceride biosynthetic process	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0045017	glycerolipid biosynthetic process	4	1
GO:0046460	neutral lipid biosynthetic process	4	1
GO:0046463	acylglycerol biosynthetic process	5	1
GO:0046467	membrane lipid biosynthetic process	4	1
GO:0046486	glycerolipid metabolic process	4	1
GO:0071704	organic substance metabolic process	2	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004144	diacylglycerol O-acyltransferase activity	7	5
GO:0008374	O-acyltransferase activity	5	9
GO:0016411	acylglycerol O-acyltransferase activity	6	5
GO:0016740	transferase activity	2	9
GO:0016746	acyltransferase activity	3	9
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	399	403	PF00656	0.598
CLV_NRD_NRD_1	149	151	PF00675	0.361
CLV_NRD_NRD_1	286	288	PF00675	0.366
CLV_NRD_NRD_1	369	371	PF00675	0.339
CLV_NRD_NRD_1	443	445	PF00675	0.306
CLV_NRD_NRD_1	479	481	PF00675	0.302
CLV_NRD_NRD_1	51	53	PF00675	0.577
CLV_NRD_NRD_1	551	553	PF00675	0.525
CLV_NRD_NRD_1	586	588	PF00675	0.591
CLV_NRD_NRD_1	92	94	PF00675	0.528
CLV_PCSK_KEX2_1	149	151	PF00082	0.405
CLV_PCSK_KEX2_1	237	239	PF00082	0.510
CLV_PCSK_KEX2_1	286	288	PF00082	0.366
CLV_PCSK_KEX2_1	369	371	PF00082	0.341
CLV_PCSK_KEX2_1	479	481	PF00082	0.311
CLV_PCSK_KEX2_1	50	52	PF00082	0.580
CLV_PCSK_KEX2_1	551	553	PF00082	0.515
CLV_PCSK_PC1ET2_1	237	239	PF00082	0.543
CLV_PCSK_PC1ET2_1	479	481	PF00082	0.337
CLV_PCSK_SKI1_1	149	153	PF00082	0.391
CLV_PCSK_SKI1_1	347	351	PF00082	0.257
CLV_PCSK_SKI1_1	369	373	PF00082	0.217
CLV_PCSK_SKI1_1	485	489	PF00082	0.301
CLV_PCSK_SKI1_1	538	542	PF00082	0.438
CLV_PCSK_SKI1_1	71	75	PF00082	0.416
DEG_APCC_DBOX_1	149	157	PF00400	0.627
DEG_Nend_UBRbox_2	1	3	PF02207	0.807
DEG_SCF_FBW7_1	258	265	PF00400	0.578
DEG_SPOP_SBC_1	174	178	PF00917	0.678
DEG_SPOP_SBC_1	262	266	PF00917	0.643
DOC_ANK_TNKS_1	112	119	PF00023	0.538
DOC_CKS1_1	187	192	PF01111	0.839
DOC_CKS1_1	21	26	PF01111	0.679
DOC_CKS1_1	280	285	PF01111	0.667
DOC_CYCLIN_RxL_1	366	375	PF00134	0.418
DOC_MAPK_gen_1	286	293	PF00069	0.597
DOC_MAPK_gen_1	469	478	PF00069	0.503
DOC_MAPK_gen_1	479	488	PF00069	0.486
DOC_MAPK_gen_1	551	558	PF00069	0.325
DOC_MAPK_HePTP_8	476	488	PF00069	0.560
DOC_MAPK_MEF2A_6	286	295	PF00069	0.544
DOC_MAPK_MEF2A_6	341	350	PF00069	0.472
DOC_MAPK_MEF2A_6	405	414	PF00069	0.537
DOC_MAPK_MEF2A_6	479	488	PF00069	0.503
DOC_MAPK_MEF2A_6	551	558	PF00069	0.301
DOC_MAPK_RevD_3	36	51	PF00069	0.598
DOC_PP1_RVXF_1	345	351	PF00149	0.418
DOC_PP2B_LxvP_1	362	365	PF13499	0.501
DOC_PP2B_LxvP_1	421	424	PF13499	0.560
DOC_PP2B_LxvP_1	562	565	PF13499	0.350
DOC_PP4_FxxP_1	332	335	PF00568	0.438
DOC_PP4_FxxP_1	530	533	PF00568	0.357
DOC_USP7_MATH_1	438	442	PF00917	0.525
DOC_USP7_MATH_1	451	455	PF00917	0.501
DOC_USP7_MATH_1	462	466	PF00917	0.471
DOC_USP7_MATH_1	75	79	PF00917	0.637
DOC_USP7_UBL2_3	481	485	PF12436	0.560
DOC_WW_Pin1_4	186	191	PF00397	0.818
DOC_WW_Pin1_4	20	25	PF00397	0.795
DOC_WW_Pin1_4	238	243	PF00397	0.689
DOC_WW_Pin1_4	258	263	PF00397	0.459
DOC_WW_Pin1_4	27	32	PF00397	0.793
DOC_WW_Pin1_4	279	284	PF00397	0.668
DOC_WW_Pin1_4	339	344	PF00397	0.490
DOC_WW_Pin1_4	489	494	PF00397	0.525
DOC_WW_Pin1_4	93	98	PF00397	0.706
LIG_14-3-3_CanoR_1	138	148	PF00244	0.633
LIG_14-3-3_CanoR_1	286	292	PF00244	0.575
LIG_14-3-3_CanoR_1	316	320	PF00244	0.360
LIG_14-3-3_CanoR_1	444	448	PF00244	0.525
LIG_14-3-3_CanoR_1	50	55	PF00244	0.748
LIG_ActinCP_TwfCPI_2	332	341	PF01115	0.438
LIG_BRCT_BRCA1_1	263	267	PF00533	0.667
LIG_BRCT_BRCA1_1	341	345	PF00533	0.480
LIG_BRCT_BRCA1_1	62	66	PF00533	0.770
LIG_DLG_GKlike_1	287	295	PF00625	0.560
LIG_EH1_1	357	365	PF00400	0.418
LIG_eIF4E_1	357	363	PF01652	0.501
LIG_FHA_1	290	296	PF00498	0.310
LIG_FHA_1	307	313	PF00498	0.450
LIG_FHA_1	316	322	PF00498	0.305
LIG_FHA_1	409	415	PF00498	0.533
LIG_FHA_1	490	496	PF00498	0.525
LIG_FHA_1	57	63	PF00498	0.770
LIG_FHA_1	570	576	PF00498	0.398
LIG_FHA_2	243	249	PF00498	0.548
LIG_FHA_2	263	269	PF00498	0.614
LIG_FHA_2	280	286	PF00498	0.579
LIG_FHA_2	569	575	PF00498	0.301
LIG_LIR_Apic_2	315	320	PF02991	0.352
LIG_LIR_Apic_2	331	335	PF02991	0.193
LIG_LIR_Gen_1	294	304	PF02991	0.364
LIG_LIR_Gen_1	342	350	PF02991	0.512
LIG_LIR_Gen_1	63	74	PF02991	0.783
LIG_LIR_LC3C_4	454	459	PF02991	0.525
LIG_LIR_LC3C_4	560	564	PF02991	0.217
LIG_LIR_Nem_3	273	279	PF02991	0.627
LIG_LIR_Nem_3	294	299	PF02991	0.481
LIG_LIR_Nem_3	342	348	PF02991	0.497
LIG_LIR_Nem_3	356	360	PF02991	0.502
LIG_LIR_Nem_3	375	381	PF02991	0.525
LIG_LIR_Nem_3	389	393	PF02991	0.525
LIG_LIR_Nem_3	513	519	PF02991	0.527
LIG_LIR_Nem_3	522	527	PF02991	0.523
LIG_LIR_Nem_3	63	69	PF02991	0.749
LIG_Pex14_1	328	332	PF04695	0.438
LIG_Pex14_1	578	582	PF04695	0.301
LIG_Pex14_2	326	330	PF04695	0.279
LIG_Pex14_2	345	349	PF04695	0.580
LIG_PTB_Apo_2	539	546	PF02174	0.438
LIG_PTB_Phospho_1	539	545	PF10480	0.438
LIG_Rb_pABgroove_1	366	374	PF01858	0.418
LIG_REV1ctd_RIR_1	347	356	PF16727	0.418
LIG_SH2_CRK	317	321	PF00017	0.433
LIG_SH2_CRK	340	344	PF00017	0.418
LIG_SH2_CRK	506	510	PF00017	0.439
LIG_SH2_CRK	545	549	PF00017	0.360
LIG_SH2_PTP2	422	425	PF00017	0.598
LIG_SH2_SRC	125	128	PF00017	0.512
LIG_SH2_SRC	162	165	PF00017	0.558
LIG_SH2_STAT5	139	142	PF00017	0.634
LIG_SH2_STAT5	257	260	PF00017	0.607
LIG_SH2_STAT5	317	320	PF00017	0.422
LIG_SH2_STAT5	325	328	PF00017	0.325
LIG_SH2_STAT5	354	357	PF00017	0.519
LIG_SH2_STAT5	378	381	PF00017	0.501
LIG_SH2_STAT5	422	425	PF00017	0.527
LIG_SH2_STAT5	519	522	PF00017	0.552
LIG_SH2_STAT5	582	585	PF00017	0.284
LIG_SH3_3	179	185	PF00018	0.789
LIG_SH3_3	18	24	PF00018	0.773
LIG_SH3_3	332	338	PF00018	0.357
LIG_SH3_3	378	384	PF00018	0.560
LIG_SH3_3	490	496	PF00018	0.523
LIG_SH3_3	555	561	PF00018	0.301
LIG_SH3_3	562	568	PF00018	0.291
LIG_SH3_3	96	102	PF00018	0.715
LIG_SUMO_SIM_anti_2	309	315	PF11976	0.366
LIG_SUMO_SIM_anti_2	454	459	PF11976	0.507
LIG_SUMO_SIM_par_1	290	297	PF11976	0.395
LIG_SUMO_SIM_par_1	309	315	PF11976	0.178
LIG_SUMO_SIM_par_1	554	560	PF11976	0.325
LIG_TRAF2_1	112	115	PF00917	0.647
LIG_TRAF2_1	487	490	PF00917	0.501
LIG_TRAF2_2	10	15	PF00917	0.656
LIG_UBA3_1	520	525	PF00899	0.431
LIG_WRC_WIRS_1	295	300	PF05994	0.448
LIG_WRC_WIRS_1	329	334	PF05994	0.422
LIG_WRC_WIRS_1	387	392	PF05994	0.391
LIG_WW_2	182	185	PF00397	0.562
MOD_CDK_SPK_2	238	243	PF00069	0.619
MOD_CDK_SPK_2	93	98	PF00069	0.585
MOD_CDK_SPxK_1	186	192	PF00069	0.830
MOD_CDK_SPxxK_3	279	286	PF00069	0.576
MOD_CK1_1	176	182	PF00069	0.704
MOD_CK1_1	263	269	PF00069	0.659
MOD_CK1_1	270	276	PF00069	0.652
MOD_CK1_1	294	300	PF00069	0.496
MOD_CK1_1	315	321	PF00069	0.492
MOD_CK1_1	60	66	PF00069	0.682
MOD_CK2_1	109	115	PF00069	0.572
MOD_CK2_1	279	285	PF00069	0.475
MOD_Cter_Amidation	235	238	PF01082	0.766
MOD_DYRK1A_RPxSP_1	20	24	PF00069	0.769
MOD_GlcNHglycan	268	272	PF01048	0.433
MOD_GlcNHglycan	437	441	PF01048	0.342
MOD_GlcNHglycan	448	451	PF01048	0.312
MOD_GlcNHglycan	77	80	PF01048	0.673
MOD_GSK3_1	173	180	PF00069	0.656
MOD_GSK3_1	194	201	PF00069	0.766
MOD_GSK3_1	238	245	PF00069	0.506
MOD_GSK3_1	258	265	PF00069	0.300
MOD_GSK3_1	275	282	PF00069	0.559
MOD_GSK3_1	287	294	PF00069	0.297
MOD_GSK3_1	56	63	PF00069	0.674
MOD_GSK3_1	85	92	PF00069	0.754
MOD_N-GLC_2	89	91	PF02516	0.579
MOD_NEK2_1	267	272	PF00069	0.503
MOD_NEK2_1	291	296	PF00069	0.357
MOD_NEK2_1	312	317	PF00069	0.500
MOD_NEK2_1	599	604	PF00069	0.430
MOD_OFUCOSY	435	442	PF10250	0.438
MOD_PKA_1	50	56	PF00069	0.630
MOD_PKA_2	315	321	PF00069	0.438
MOD_PKA_2	333	339	PF00069	0.434
MOD_PKA_2	443	449	PF00069	0.357
MOD_PKA_2	50	56	PF00069	0.747
MOD_PKA_2	75	81	PF00069	0.741
MOD_Plk_1	34	40	PF00069	0.517
MOD_Plk_1	462	468	PF00069	0.438
MOD_Plk_4	275	281	PF00069	0.583
MOD_Plk_4	291	297	PF00069	0.306
MOD_Plk_4	341	347	PF00069	0.403
MOD_Plk_4	451	457	PF00069	0.378
MOD_Plk_4	462	468	PF00069	0.315
MOD_ProDKin_1	186	192	PF00069	0.801
MOD_ProDKin_1	20	26	PF00069	0.770
MOD_ProDKin_1	238	244	PF00069	0.604
MOD_ProDKin_1	258	264	PF00069	0.295
MOD_ProDKin_1	27	33	PF00069	0.761
MOD_ProDKin_1	279	285	PF00069	0.587
MOD_ProDKin_1	339	345	PF00069	0.341
MOD_ProDKin_1	489	495	PF00069	0.391
MOD_ProDKin_1	93	99	PF00069	0.641
MOD_SUMO_rev_2	142	148	PF00179	0.558
TRG_DiLeu_BaLyEn_6	246	251	PF01217	0.513
TRG_DiLeu_BaLyEn_6	358	363	PF01217	0.240
TRG_ENDOCYTIC_2	357	360	PF00928	0.335
TRG_ENDOCYTIC_2	378	381	PF00928	0.357
TRG_ENDOCYTIC_2	422	425	PF00928	0.397
TRG_ENDOCYTIC_2	545	548	PF00928	0.438
TRG_ER_diArg_1	136	139	PF00400	0.577
TRG_ER_diArg_1	148	150	PF00400	0.460
TRG_ER_diArg_1	286	288	PF00400	0.448
TRG_ER_diArg_1	368	370	PF00400	0.412
TRG_ER_diArg_1	50	52	PF00400	0.637
TRG_ER_diArg_1	551	553	PF00400	0.391
TRG_Pf-PMV_PEXEL_1	369	373	PF00026	0.240
TRG_Pf-PMV_PEXEL_1	518	522	PF00026	0.240
TRG_Pf-PMV_PEXEL_1	52	57	PF00026	0.705
TRG_Pf-PMV_PEXEL_1	587	592	PF00026	0.492
TRG_Pf-PMV_PEXEL_1	594	598	PF00026	0.377

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PE36	Leptomonas seymouri	57%	93%
A0A3R7NQK7	Trypanosoma rangeli	44%	100%
A0A3S7WZ82	Leishmania donovani	78%	100%
A4I1K9	Leishmania infantum	77%	100%
C9ZK51	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AXP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	99%
Q4Q9S8	Leishmania major	76%	100%