3-oxo-5-alpha-steroid 4-dehydrogenase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase, putative

Species:

Leishmania braziliensis

UniProt:

A4HEG5_LEIBR

TriTrypDb:

LbrM.25.2220 , LBRM2903_250026700

Length:

309

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	1
GO:0005777	peroxisome	6	1
GO:0020015	glycosome	7	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0031090	organelle membrane	3	1

Expansion

Sequence features

A4HEG5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HEG5

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0000038	very long-chain fatty acid metabolic process	5	1
GO:0006082	organic acid metabolic process	3	1
GO:0006631	fatty acid metabolic process	4	1
GO:0006633	fatty acid biosynthetic process	5	1
GO:0008610	lipid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	1
GO:0016053	organic acid biosynthetic process	4	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0032787	monocarboxylic acid metabolic process	6	1
GO:0042761	very long-chain fatty acid biosynthetic process	6	1
GO:0043436	oxoacid metabolic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044281	small molecule metabolic process	2	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046394	carboxylic acid biosynthetic process	5	1
GO:0072330	monocarboxylic acid biosynthetic process	6	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	5
GO:0016229	steroid dehydrogenase activity	3	5
GO:0016491	oxidoreductase activity	2	11
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	11
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	5
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	1
GO:0102758	very-long-chain enoyl-CoA reductase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	7	11	PF00656	0.633
CLV_NRD_NRD_1	12	14	PF00675	0.449
CLV_NRD_NRD_1	225	227	PF00675	0.431
CLV_NRD_NRD_1	39	41	PF00675	0.278
CLV_PCSK_KEX2_1	149	151	PF00082	0.233
CLV_PCSK_PC1ET2_1	149	151	PF00082	0.233
CLV_PCSK_SKI1_1	168	172	PF00082	0.261
CLV_PCSK_SKI1_1	41	45	PF00082	0.265
CLV_PCSK_SKI1_1	99	103	PF00082	0.214
DEG_APCC_DBOX_1	63	71	PF00400	0.627
DEG_Nend_UBRbox_1	1	4	PF02207	0.485
DOC_MAPK_gen_1	13	19	PF00069	0.576
DOC_MAPK_gen_1	149	157	PF00069	0.477
DOC_MAPK_gen_1	29	38	PF00069	0.440
DOC_MAPK_gen_1	299	307	PF00069	0.424
DOC_MAPK_gen_1	62	70	PF00069	0.601
DOC_MAPK_MEF2A_6	137	144	PF00069	0.382
DOC_MAPK_NFAT4_5	137	145	PF00069	0.382
DOC_PP4_FxxP_1	160	163	PF00568	0.492
DOC_PP4_FxxP_1	43	46	PF00568	0.527
DOC_USP7_MATH_1	198	202	PF00917	0.222
DOC_USP7_MATH_1	243	247	PF00917	0.309
DOC_USP7_MATH_1	31	35	PF00917	0.530
DOC_USP7_MATH_1	51	55	PF00917	0.622
DOC_WW_Pin1_4	187	192	PF00397	0.373
LIG_14-3-3_CanoR_1	40	44	PF00244	0.479
LIG_BRCT_BRCA1_1	189	193	PF00533	0.301
LIG_BRCT_BRCA1_1	267	271	PF00533	0.251
LIG_FHA_1	133	139	PF00498	0.268
LIG_FHA_1	143	149	PF00498	0.296
LIG_FHA_1	231	237	PF00498	0.226
LIG_FHA_1	262	268	PF00498	0.291
LIG_FHA_2	22	28	PF00498	0.490
LIG_FHA_2	67	73	PF00498	0.560
LIG_FHA_2	86	92	PF00498	0.400
LIG_GBD_Chelix_1	110	118	PF00786	0.382
LIG_LIR_Apic_2	105	109	PF02991	0.249
LIG_LIR_Apic_2	42	46	PF02991	0.523
LIG_LIR_Gen_1	179	188	PF02991	0.313
LIG_LIR_Gen_1	256	262	PF02991	0.262
LIG_LIR_Nem_3	100	106	PF02991	0.465
LIG_LIR_Nem_3	179	183	PF02991	0.343
LIG_LIR_Nem_3	201	206	PF02991	0.261
LIG_LIR_Nem_3	256	260	PF02991	0.262
LIG_LIR_Nem_3	268	274	PF02991	0.289
LIG_LIR_Nem_3	88	92	PF02991	0.464
LIG_MLH1_MIPbox_1	189	193	PF16413	0.215
LIG_MLH1_MIPbox_1	268	272	PF16413	0.349
LIG_PALB2_WD40_1	266	274	PF16756	0.248
LIG_PCNA_yPIPBox_3	234	245	PF02747	0.303
LIG_Pex14_2	143	147	PF04695	0.325
LIG_Pex14_2	156	160	PF04695	0.501
LIG_Pex14_2	203	207	PF04695	0.206
LIG_PTB_Apo_2	170	177	PF02174	0.414
LIG_PTB_Phospho_1	170	176	PF10480	0.414
LIG_SH2_CRK	106	110	PF00017	0.249
LIG_SH2_CRK	98	102	PF00017	0.455
LIG_SH2_PTP2	139	142	PF00017	0.327
LIG_SH2_STAP1	134	138	PF00017	0.268
LIG_SH2_STAT5	103	106	PF00017	0.314
LIG_SH2_STAT5	134	137	PF00017	0.275
LIG_SH2_STAT5	139	142	PF00017	0.263
LIG_SH2_STAT5	186	189	PF00017	0.261
LIG_SH2_STAT5	250	253	PF00017	0.214
LIG_SH2_STAT5	291	294	PF00017	0.428
LIG_SH3_1	234	240	PF00018	0.245
LIG_SH3_2	163	168	PF14604	0.445
LIG_SH3_3	160	166	PF00018	0.479
LIG_SH3_3	234	240	PF00018	0.225
LIG_SH3_3	242	248	PF00018	0.202
LIG_SH3_3	42	48	PF00018	0.502
LIG_SUMO_SIM_par_1	66	72	PF11976	0.548
LIG_TYR_ITIM	87	92	PF00017	0.483
LIG_TYR_ITIM	96	101	PF00017	0.439
LIG_UBA3_1	67	73	PF00899	0.602
LIG_WRC_WIRS_1	177	182	PF05994	0.249
MOD_CK1_1	161	167	PF00069	0.414
MOD_CK1_1	256	262	PF00069	0.312
MOD_CK1_1	54	60	PF00069	0.612
MOD_CK2_1	66	72	PF00069	0.461
MOD_GlcNHglycan	125	128	PF01048	0.587
MOD_GlcNHglycan	196	199	PF01048	0.455
MOD_GlcNHglycan	53	56	PF01048	0.383
MOD_GlcNHglycan	6	9	PF01048	0.342
MOD_GSK3_1	187	194	PF00069	0.390
MOD_GSK3_1	221	228	PF00069	0.321
MOD_GSK3_1	261	268	PF00069	0.261
MOD_GSK3_1	54	61	PF00069	0.621
MOD_N-GLC_1	21	26	PF02516	0.304
MOD_NEK2_1	142	147	PF00069	0.255
MOD_NEK2_1	193	198	PF00069	0.225
MOD_NEK2_1	266	271	PF00069	0.260
MOD_NEK2_2	97	102	PF00069	0.455
MOD_PIKK_1	132	138	PF00454	0.268
MOD_PIKK_1	74	80	PF00454	0.588
MOD_PKA_2	118	124	PF00069	0.257
MOD_PKA_2	225	231	PF00069	0.261
MOD_PKA_2	39	45	PF00069	0.489
MOD_Plk_1	21	27	PF00069	0.520
MOD_Plk_4	118	124	PF00069	0.257
MOD_Plk_4	151	157	PF00069	0.471
MOD_Plk_4	253	259	PF00069	0.229
MOD_Plk_4	266	272	PF00069	0.214
MOD_Plk_4	82	88	PF00069	0.612
MOD_ProDKin_1	187	193	PF00069	0.373
MOD_SUMO_for_1	148	151	PF00179	0.440
TRG_ENDOCYTIC_2	139	142	PF00928	0.327
TRG_ENDOCYTIC_2	177	180	PF00928	0.249
TRG_ENDOCYTIC_2	89	92	PF00928	0.487
TRG_ENDOCYTIC_2	98	101	PF00928	0.441
TRG_NLS_Bipartite_1	285	303	PF00514	0.447
TRG_NLS_MonoExtC_3	284	289	PF00514	0.509
TRG_NLS_MonoExtN_4	285	290	PF00514	0.489
TRG_NLS_MonoExtN_4	296	303	PF00514	0.441

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF75	Leptomonas seymouri	74%	100%
A0A0S4IKY4	Bodo saltans	56%	100%
A0A1X0NYG0	Trypanosomatidae	60%	100%
A0A3Q8INK5	Leishmania donovani	89%	100%
A0A422NGL4	Trypanosoma rangeli	60%	100%
A4I1M5	Leishmania infantum	89%	100%
C9ZK67	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
E9AXQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
O94511	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
Q3SZ89	Bos taurus	34%	85%
Q3ZCD7	Bos taurus	39%	100%
Q4Q9R2	Leishmania major	90%	99%
Q55C17	Dictyostelium discoideum	36%	100%
Q5HYJ1	Homo sapiens	32%	85%
Q64232	Rattus norvegicus	39%	100%
Q68FF9	Mus musculus	22%	100%
Q8BFZ1	Mus musculus	34%	86%
Q99190	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q9CY27	Mus musculus	39%	100%
Q9M2U2	Arabidopsis thaliana	32%	100%
Q9N5Y2	Caenorhabditis elegans	42%	100%
Q9NZ01	Homo sapiens	39%	100%