Putative pumillio protein 8

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Pumilio homology domain family member 8

Species:

Leishmania braziliensis

UniProt:

A4HED9_LEIBR

TriTrypDb:

LbrM.25.1950 , LBRM2903_250032800

Length:

577

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HED9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HED9

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0034248	regulation of amide metabolic process	5	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003729	mRNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	452	456	PF00656	0.355
CLV_NRD_NRD_1	237	239	PF00675	0.459
CLV_NRD_NRD_1	313	315	PF00675	0.427
CLV_NRD_NRD_1	385	387	PF00675	0.412
CLV_NRD_NRD_1	541	543	PF00675	0.746
CLV_NRD_NRD_1	560	562	PF00675	0.783
CLV_PCSK_KEX2_1	217	219	PF00082	0.431
CLV_PCSK_KEX2_1	237	239	PF00082	0.394
CLV_PCSK_KEX2_1	313	315	PF00082	0.446
CLV_PCSK_KEX2_1	387	389	PF00082	0.523
CLV_PCSK_KEX2_1	503	505	PF00082	0.654
CLV_PCSK_KEX2_1	560	562	PF00082	0.797
CLV_PCSK_PC1ET2_1	217	219	PF00082	0.400
CLV_PCSK_PC1ET2_1	313	315	PF00082	0.446
CLV_PCSK_PC1ET2_1	387	389	PF00082	0.423
CLV_PCSK_PC1ET2_1	503	505	PF00082	0.657
CLV_PCSK_SKI1_1	165	169	PF00082	0.443
CLV_PCSK_SKI1_1	201	205	PF00082	0.344
CLV_PCSK_SKI1_1	237	241	PF00082	0.377
CLV_PCSK_SKI1_1	24	28	PF00082	0.458
CLV_PCSK_SKI1_1	315	319	PF00082	0.417
CLV_PCSK_SKI1_1	388	392	PF00082	0.443
CLV_PCSK_SKI1_1	443	447	PF00082	0.426
CLV_PCSK_SKI1_1	468	472	PF00082	0.406
CLV_PCSK_SKI1_1	543	547	PF00082	0.705
CLV_PCSK_SKI1_1	560	564	PF00082	0.757
CLV_Separin_Metazoa	55	59	PF03568	0.437
DEG_APCC_DBOX_1	236	244	PF00400	0.527
DEG_APCC_DBOX_1	467	475	PF00400	0.479
DEG_SCF_FBW7_1	105	112	PF00400	0.409
DEG_SCF_FBW7_2	379	385	PF00400	0.399
DOC_CDC14_PxL_1	171	179	PF14671	0.388
DOC_CDC14_PxL_1	435	443	PF14671	0.432
DOC_CKS1_1	106	111	PF01111	0.418
DOC_CKS1_1	379	384	PF01111	0.374
DOC_CYCLIN_RxL_1	234	241	PF00134	0.449
DOC_MAPK_MEF2A_6	201	208	PF00069	0.367
DOC_MAPK_NFAT4_5	201	209	PF00069	0.367
DOC_PP1_RVXF_1	91	98	PF00149	0.284
DOC_PP4_FxxP_1	390	393	PF00568	0.451
DOC_PP4_FxxP_1	97	100	PF00568	0.303
DOC_USP7_MATH_1	109	113	PF00917	0.396
DOC_USP7_MATH_1	199	203	PF00917	0.455
DOC_USP7_MATH_1	300	304	PF00917	0.416
DOC_USP7_MATH_1	430	434	PF00917	0.468
DOC_USP7_MATH_1	449	453	PF00917	0.507
DOC_USP7_MATH_1	517	521	PF00917	0.565
DOC_USP7_MATH_1	74	78	PF00917	0.658
DOC_USP7_MATH_2	159	165	PF00917	0.556
DOC_USP7_UBL2_3	132	136	PF12436	0.514
DOC_USP7_UBL2_3	44	48	PF12436	0.501
DOC_USP7_UBL2_3	563	567	PF12436	0.750
DOC_WW_Pin1_4	105	110	PF00397	0.406
DOC_WW_Pin1_4	194	199	PF00397	0.521
DOC_WW_Pin1_4	366	371	PF00397	0.372
DOC_WW_Pin1_4	378	383	PF00397	0.368
LIG_14-3-3_CanoR_1	113	119	PF00244	0.387
LIG_Actin_WH2_2	239	256	PF00022	0.401
LIG_Actin_WH2_2	431	449	PF00022	0.390
LIG_APCC_ABBA_1	206	211	PF00400	0.428
LIG_APCC_ABBA_1	380	385	PF00400	0.444
LIG_BRCT_BRCA1_1	134	138	PF00533	0.428
LIG_BRCT_BRCA1_1	302	306	PF00533	0.407
LIG_Clathr_ClatBox_1	400	404	PF01394	0.452
LIG_deltaCOP1_diTrp_1	407	413	PF00928	0.461
LIG_EH1_1	148	156	PF00400	0.353
LIG_eIF4E_1	371	377	PF01652	0.446
LIG_FHA_1	106	112	PF00498	0.445
LIG_FHA_1	166	172	PF00498	0.479
LIG_FHA_1	420	426	PF00498	0.435
LIG_FHA_1	430	436	PF00498	0.403
LIG_FHA_2	272	278	PF00498	0.409
LIG_FHA_2	379	385	PF00498	0.412
LIG_FHA_2	508	514	PF00498	0.500
LIG_FHA_2	535	541	PF00498	0.774
LIG_FHA_2	63	69	PF00498	0.539
LIG_HCF-1_HBM_1	85	88	PF13415	0.350
LIG_Integrin_isoDGR_2	528	530	PF01839	0.485
LIG_LIR_Apic_2	192	198	PF02991	0.514
LIG_LIR_Apic_2	330	336	PF02991	0.391
LIG_LIR_Gen_1	143	154	PF02991	0.437
LIG_LIR_Gen_1	161	171	PF02991	0.329
LIG_LIR_Gen_1	99	109	PF02991	0.531
LIG_LIR_Nem_3	135	141	PF02991	0.401
LIG_LIR_Nem_3	143	149	PF02991	0.339
LIG_LIR_Nem_3	161	166	PF02991	0.398
LIG_LIR_Nem_3	85	91	PF02991	0.381
LIG_LIR_Nem_3	99	105	PF02991	0.462
LIG_LYPXL_yS_3	222	225	PF13949	0.404
LIG_LYPXL_yS_3	259	262	PF13949	0.435
LIG_LYPXL_yS_3	88	91	PF13949	0.404
LIG_NRBOX	340	346	PF00104	0.474
LIG_NRBOX	375	381	PF00104	0.395
LIG_PCNA_yPIPBox_3	263	275	PF02747	0.397
LIG_PDZ_Class_1	572	577	PF00595	0.522
LIG_PTB_Apo_2	365	372	PF02174	0.375
LIG_PTB_Phospho_1	365	371	PF10480	0.464
LIG_Rb_pABgroove_1	238	246	PF01858	0.461
LIG_Rb_pABgroove_1	336	344	PF01858	0.340
LIG_RPA_C_Fungi	455	467	PF08784	0.545
LIG_SH2_CRK	163	167	PF00017	0.468
LIG_SH2_CRK	333	337	PF00017	0.348
LIG_SH2_CRK	373	377	PF00017	0.448
LIG_SH2_STAP1	141	145	PF00017	0.424
LIG_SH2_STAP1	146	150	PF00017	0.412
LIG_SH2_STAP1	163	167	PF00017	0.504
LIG_SH2_STAP1	191	195	PF00017	0.392
LIG_SH2_STAP1	346	350	PF00017	0.398
LIG_SH2_STAP1	84	88	PF00017	0.475
LIG_SH2_STAT3	346	349	PF00017	0.418
LIG_SH2_STAT3	371	374	PF00017	0.483
LIG_SH2_STAT5	269	272	PF00017	0.401
LIG_SH2_STAT5	354	357	PF00017	0.362
LIG_SH3_1	217	223	PF00018	0.556
LIG_SH3_3	103	109	PF00018	0.439
LIG_SH3_3	169	175	PF00018	0.369
LIG_SH3_3	217	223	PF00018	0.551
LIG_SH3_3	364	370	PF00018	0.524
LIG_SUMO_SIM_anti_2	202	207	PF11976	0.391
LIG_SUMO_SIM_anti_2	211	217	PF11976	0.355
LIG_SUMO_SIM_par_1	248	255	PF11976	0.429
LIG_SUMO_SIM_par_1	421	427	PF11976	0.462
LIG_TRAF2_1	11	14	PF00917	0.704
LIG_TRAF2_1	274	277	PF00917	0.294
LIG_TRAF2_1	382	385	PF00917	0.486
LIG_TRAF2_1	488	491	PF00917	0.560
LIG_TRAF2_1	509	512	PF00917	0.683
LIG_TRAF2_1	65	68	PF00917	0.601
LIG_TYR_ITIM	257	262	PF00017	0.426
LIG_UBA3_1	203	210	PF00899	0.359
LIG_UBA3_1	243	249	PF00899	0.387
LIG_UBA3_1	400	406	PF00899	0.491
LIG_UBA3_1	94	101	PF00899	0.421
MOD_CDK_SPxxK_3	194	201	PF00069	0.514
MOD_CK1_1	189	195	PF00069	0.449
MOD_CK2_1	249	255	PF00069	0.483
MOD_CK2_1	271	277	PF00069	0.415
MOD_CK2_1	378	384	PF00069	0.429
MOD_CK2_1	484	490	PF00069	0.520
MOD_CK2_1	507	513	PF00069	0.660
MOD_CK2_1	521	527	PF00069	0.717
MOD_CK2_1	62	68	PF00069	0.586
MOD_Cter_Amidation	501	504	PF01082	0.723
MOD_Cter_Amidation	558	561	PF01082	0.771
MOD_GlcNHglycan	122	125	PF01048	0.529
MOD_GlcNHglycan	27	30	PF01048	0.404
MOD_GlcNHglycan	415	418	PF01048	0.557
MOD_GlcNHglycan	451	454	PF01048	0.489
MOD_GlcNHglycan	486	489	PF01048	0.558
MOD_GlcNHglycan	519	522	PF01048	0.578
MOD_GlcNHglycan	574	577	PF01048	0.630
MOD_GlcNHglycan	76	79	PF01048	0.436
MOD_GSK3_1	101	108	PF00069	0.390
MOD_GSK3_1	132	139	PF00069	0.506
MOD_GSK3_1	161	168	PF00069	0.504
MOD_GSK3_1	186	193	PF00069	0.388
MOD_GSK3_1	300	307	PF00069	0.437
MOD_GSK3_1	409	416	PF00069	0.488
MOD_GSK3_1	43	50	PF00069	0.386
MOD_GSK3_1	517	524	PF00069	0.479
MOD_GSK3_1	567	574	PF00069	0.605
MOD_NEK2_1	1	6	PF00069	0.603
MOD_NEK2_1	209	214	PF00069	0.406
MOD_NEK2_1	25	30	PF00069	0.410
MOD_NEK2_1	265	270	PF00069	0.448
MOD_NEK2_1	281	286	PF00069	0.269
MOD_NEK2_1	304	309	PF00069	0.414
MOD_NEK2_1	328	333	PF00069	0.439
MOD_NEK2_1	479	484	PF00069	0.435
MOD_PK_1	107	113	PF00069	0.546
MOD_PKA_1	567	573	PF00069	0.702
MOD_PKA_2	14	20	PF00069	0.632
MOD_Plk_1	136	142	PF00069	0.495
MOD_Plk_1	199	205	PF00069	0.486
MOD_Plk_1	67	73	PF00069	0.603
MOD_Plk_2-3	507	513	PF00069	0.507
MOD_Plk_2-3	67	73	PF00069	0.671
MOD_Plk_4	101	107	PF00069	0.354
MOD_Plk_4	114	120	PF00069	0.416
MOD_Plk_4	126	132	PF00069	0.375
MOD_Plk_4	186	192	PF00069	0.451
MOD_Plk_4	265	271	PF00069	0.516
MOD_Plk_4	409	415	PF00069	0.415
MOD_ProDKin_1	105	111	PF00069	0.401
MOD_ProDKin_1	194	200	PF00069	0.517
MOD_ProDKin_1	366	372	PF00069	0.368
MOD_ProDKin_1	378	384	PF00069	0.372
MOD_SUMO_rev_2	451	458	PF00179	0.347
TRG_DiLeu_BaEn_4	276	282	PF01217	0.287
TRG_DiLeu_BaLyEn_6	235	240	PF01217	0.399
TRG_ENDOCYTIC_2	146	149	PF00928	0.376
TRG_ENDOCYTIC_2	163	166	PF00928	0.333
TRG_ENDOCYTIC_2	222	225	PF00928	0.392
TRG_ENDOCYTIC_2	259	262	PF00928	0.418
TRG_ENDOCYTIC_2	373	376	PF00928	0.448
TRG_ENDOCYTIC_2	88	91	PF00928	0.404
TRG_ER_diArg_1	237	239	PF00400	0.455
TRG_ER_diArg_1	560	562	PF00400	0.797
TRG_NLS_MonoExtC_3	541	546	PF00514	0.755
TRG_NLS_MonoExtN_4	386	391	PF00514	0.407
TRG_NLS_MonoExtN_4	542	547	PF00514	0.782
TRG_Pf-PMV_PEXEL_1	64	68	PF00026	0.571

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HWV1	Leptomonas seymouri	78%	100%
A0A0S4IXV1	Bodo saltans	45%	82%
A0A1X0NZF1	Trypanosomatidae	58%	94%
A0A3Q8IDL8	Leishmania donovani	85%	100%
A0A3R7MC19	Trypanosoma rangeli	55%	99%
A4I1T6	Leishmania infantum	85%	100%
C9ZKE5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	95%
E9AXX0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q4Q9K0	Leishmania major	84%	100%
V5D8H4	Trypanosoma cruzi	53%	99%