Not1 N-terminal domain, CCR4-Not complex component-domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

General negative regulator of transcription subunit 5

Species:

Leishmania braziliensis

UniProt:

A4HE86_LEIBR

TriTrypDb:

LbrM.25.1400 , LBRM2903_250027400

Length:

643

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	8
GO:0005737	cytoplasm	2	8
GO:0030015	CCR4-NOT core complex	3	8
GO:0032991	protein-containing complex	1	8
GO:0043226	organelle	2	8
GO:0043227	membrane-bounded organelle	3	8
GO:0043229	intracellular organelle	3	8
GO:0043231	intracellular membrane-bounded organelle	4	8
GO:0110165	cellular anatomical entity	1	8
GO:0140535	intracellular protein-containing complex	2	8
GO:0000932	P-body	5	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1

Expansion

Sequence features

A4HE86

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HE86

Function

Biological processes
Term	Name	Level	Count
GO:0006355	regulation of DNA-templated transcription	6	8
GO:0009889	regulation of biosynthetic process	4	8
GO:0010468	regulation of gene expression	5	8
GO:0010556	regulation of macromolecule biosynthetic process	5	8
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	8
GO:0019222	regulation of metabolic process	3	8
GO:0031323	regulation of cellular metabolic process	4	8
GO:0031326	regulation of cellular biosynthetic process	5	8
GO:0050789	regulation of biological process	2	8
GO:0050794	regulation of cellular process	3	8
GO:0051171	regulation of nitrogen compound metabolic process	4	8
GO:0051252	regulation of RNA metabolic process	5	8
GO:0060255	regulation of macromolecule metabolic process	4	8
GO:0065007	biological regulation	1	8
GO:0080090	regulation of primary metabolic process	4	8
GO:1903506	regulation of nucleic acid-templated transcription	7	8
GO:2001141	regulation of RNA biosynthetic process	6	8
GO:0000289	nuclear-transcribed mRNA poly(A) tail shortening	8	1
GO:0000956	nuclear-transcribed mRNA catabolic process	7	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006401	RNA catabolic process	5	1
GO:0006402	mRNA catabolic process	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009892	negative regulation of metabolic process	4	1
GO:0009987	cellular process	1	1
GO:0010605	negative regulation of macromolecule metabolic process	5	1
GO:0010629	negative regulation of gene expression	6	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046700	heterocycle catabolic process	4	1
GO:0048519	negative regulation of biological process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.581
CLV_C14_Caspase3-7	20	24	PF00656	0.522
CLV_C14_Caspase3-7	312	316	PF00656	0.582
CLV_NRD_NRD_1	131	133	PF00675	0.293
CLV_NRD_NRD_1	151	153	PF00675	0.221
CLV_NRD_NRD_1	413	415	PF00675	0.569
CLV_NRD_NRD_1	80	82	PF00675	0.333
CLV_PCSK_FUR_1	149	153	PF00082	0.333
CLV_PCSK_KEX2_1	151	153	PF00082	0.333
CLV_PCSK_KEX2_1	413	415	PF00082	0.595
CLV_PCSK_KEX2_1	49	51	PF00082	0.293
CLV_PCSK_PC1ET2_1	49	51	PF00082	0.333
CLV_PCSK_SKI1_1	13	17	PF00082	0.333
CLV_PCSK_SKI1_1	381	385	PF00082	0.896
CLV_PCSK_SKI1_1	50	54	PF00082	0.333
CLV_PCSK_SKI1_1	579	583	PF00082	0.377
CLV_PCSK_SKI1_1	96	100	PF00082	0.343
DEG_Kelch_Keap1_1	315	320	PF01344	0.583
DEG_SCF_FBW7_1	472	478	PF00400	0.847
DEG_SPOP_SBC_1	248	252	PF00917	0.575
DEG_SPOP_SBC_1	475	479	PF00917	0.574
DEG_SPOP_SBC_1	602	606	PF00917	0.545
DOC_CKS1_1	441	446	PF01111	0.581
DOC_CKS1_1	460	465	PF01111	0.606
DOC_CKS1_1	472	477	PF01111	0.832
DOC_CYCLIN_yClb5_NLxxxL_5	510	519	PF00134	0.377
DOC_CYCLIN_yCln2_LP_2	399	405	PF00134	0.600
DOC_MAPK_gen_1	228	235	PF00069	0.651
DOC_MAPK_gen_1	72	79	PF00069	0.522
DOC_PP2B_LxvP_1	399	402	PF13499	0.581
DOC_PP2B_LxvP_1	464	467	PF13499	0.624
DOC_PP4_FxxP_1	222	225	PF00568	0.522
DOC_USP7_MATH_1	282	286	PF00917	0.614
DOC_USP7_MATH_1	300	304	PF00917	0.741
DOC_USP7_MATH_1	36	40	PF00917	0.533
DOC_USP7_MATH_1	452	456	PF00917	0.679
DOC_USP7_MATH_1	475	479	PF00917	0.836
DOC_USP7_MATH_1	483	487	PF00917	0.661
DOC_USP7_UBL2_3	249	253	PF12436	0.742
DOC_USP7_UBL2_3	49	53	PF12436	0.533
DOC_USP7_UBL2_3	498	502	PF12436	0.550
DOC_USP7_UBL2_3	72	76	PF12436	0.533
DOC_WW_Pin1_4	244	249	PF00397	0.635
DOC_WW_Pin1_4	253	258	PF00397	0.646
DOC_WW_Pin1_4	263	268	PF00397	0.617
DOC_WW_Pin1_4	275	280	PF00397	0.607
DOC_WW_Pin1_4	285	290	PF00397	0.726
DOC_WW_Pin1_4	374	379	PF00397	0.694
DOC_WW_Pin1_4	404	409	PF00397	0.685
DOC_WW_Pin1_4	440	445	PF00397	0.624
DOC_WW_Pin1_4	459	464	PF00397	0.603
DOC_WW_Pin1_4	468	473	PF00397	0.651
DOC_WW_Pin1_4	497	502	PF00397	0.744
LIG_14-3-3_CanoR_1	396	402	PF00244	0.738
LIG_14-3-3_CanoR_1	611	617	PF00244	0.533
LIG_BIR_II_1	1	5	PF00653	0.578
LIG_BRCT_BRCA1_1	612	616	PF00533	0.533
LIG_EVH1_1	487	491	PF00568	0.639
LIG_FHA_1	188	194	PF00498	0.525
LIG_FHA_1	219	225	PF00498	0.551
LIG_FHA_1	359	365	PF00498	0.629
LIG_FHA_1	380	386	PF00498	0.699
LIG_FHA_1	460	466	PF00498	0.671
LIG_FHA_1	479	485	PF00498	0.802
LIG_FHA_1	516	522	PF00498	0.515
LIG_FHA_2	332	338	PF00498	0.647
LIG_FHA_2	390	396	PF00498	0.642
LIG_FHA_2	477	483	PF00498	0.794
LIG_FHA_2	602	608	PF00498	0.539
LIG_FHA_2	613	619	PF00498	0.520
LIG_FHA_2	7	13	PF00498	0.507
LIG_Integrin_isoDGR_2	147	149	PF01839	0.333
LIG_LIR_Apic_2	220	225	PF02991	0.522
LIG_LIR_Apic_2	367	371	PF02991	0.574
LIG_LIR_Gen_1	217	227	PF02991	0.494
LIG_LIR_Gen_1	23	33	PF02991	0.494
LIG_LIR_Gen_1	551	561	PF02991	0.577
LIG_LIR_Gen_1	613	619	PF02991	0.533
LIG_LIR_Nem_3	217	222	PF02991	0.494
LIG_LIR_Nem_3	23	28	PF02991	0.494
LIG_LIR_Nem_3	486	490	PF02991	0.708
LIG_LIR_Nem_3	559	565	PF02991	0.531
LIG_LIR_Nem_3	571	575	PF02991	0.540
LIG_LIR_Nem_3	613	619	PF02991	0.495
LIG_LIR_Nem_3	634	638	PF02991	0.530
LIG_LIR_Nem_3	89	94	PF02991	0.533
LIG_MAD2	363	371	PF02301	0.557
LIG_Pex14_2	619	623	PF04695	0.493
LIG_PTB_Apo_2	65	72	PF02174	0.533
LIG_PTB_Phospho_1	65	71	PF10480	0.533
LIG_REV1ctd_RIR_1	171	179	PF16727	0.522
LIG_SH2_NCK_1	527	531	PF00017	0.533
LIG_SH2_SRC	527	530	PF00017	0.533
LIG_SH2_STAT3	140	143	PF00017	0.558
LIG_SH2_STAT3	538	541	PF00017	0.533
LIG_SH2_STAT3	566	569	PF00017	0.493
LIG_SH2_STAT5	538	541	PF00017	0.555
LIG_SH2_STAT5	575	578	PF00017	0.493
LIG_SH2_STAT5	614	617	PF00017	0.533
LIG_SH2_STAT5	637	640	PF00017	0.556
LIG_SH3_1	523	529	PF00018	0.533
LIG_SH3_3	242	248	PF00018	0.594
LIG_SH3_3	273	279	PF00018	0.538
LIG_SH3_3	286	292	PF00018	0.570
LIG_SH3_3	368	374	PF00018	0.654
LIG_SH3_3	438	444	PF00018	0.680
LIG_SH3_3	457	463	PF00018	0.650
LIG_SH3_3	469	475	PF00018	0.731
LIG_SH3_3	485	491	PF00018	0.748
LIG_SH3_3	523	529	PF00018	0.510
LIG_SUMO_SIM_anti_2	209	215	PF11976	0.522
LIG_TRAF2_1	127	130	PF00917	0.612
LIG_WRC_WIRS_1	219	224	PF05994	0.533
LIG_WRC_WIRS_1	62	67	PF05994	0.522
LIG_WRC_WIRS_1	88	93	PF05994	0.493
MOD_CDK_SPK_2	244	249	PF00069	0.691
MOD_CDK_SPK_2	497	502	PF00069	0.556
MOD_CDK_SPxK_1	497	503	PF00069	0.547
MOD_CDK_SPxxK_3	374	381	PF00069	0.659
MOD_CK1_1	247	253	PF00069	0.675
MOD_CK1_1	285	291	PF00069	0.569
MOD_CK1_1	445	451	PF00069	0.653
MOD_CK1_1	471	477	PF00069	0.689
MOD_CK1_1	478	484	PF00069	0.643
MOD_CK1_1	497	503	PF00069	0.406
MOD_CK2_1	115	121	PF00069	0.533
MOD_CK2_1	235	241	PF00069	0.662
MOD_CK2_1	268	274	PF00069	0.654
MOD_CK2_1	350	356	PF00069	0.662
MOD_CK2_1	36	42	PF00069	0.533
MOD_CK2_1	389	395	PF00069	0.694
MOD_CK2_1	452	458	PF00069	0.786
MOD_CK2_1	601	607	PF00069	0.533
MOD_Cter_Amidation	130	133	PF01082	0.333
MOD_GlcNHglycan	109	112	PF01048	0.321
MOD_GlcNHglycan	117	120	PF01048	0.360
MOD_GlcNHglycan	298	301	PF01048	0.601
MOD_GlcNHglycan	38	41	PF01048	0.333
MOD_GlcNHglycan	447	450	PF01048	0.656
MOD_GSK3_1	214	221	PF00069	0.533
MOD_GSK3_1	244	251	PF00069	0.794
MOD_GSK3_1	253	260	PF00069	0.778
MOD_GSK3_1	263	270	PF00069	0.752
MOD_GSK3_1	296	303	PF00069	0.671
MOD_GSK3_1	304	311	PF00069	0.841
MOD_GSK3_1	343	350	PF00069	0.797
MOD_GSK3_1	379	386	PF00069	0.658
MOD_GSK3_1	467	474	PF00069	0.704
MOD_GSK3_1	601	608	PF00069	0.545
MOD_GSK3_1	97	104	PF00069	0.508
MOD_N-GLC_1	36	41	PF02516	0.298
MOD_N-GLC_1	433	438	PF02516	0.551
MOD_NEK2_1	268	273	PF00069	0.612
MOD_NEK2_1	508	513	PF00069	0.526
MOD_NEK2_1	77	82	PF00069	0.533
MOD_NEK2_2	612	617	PF00069	0.533
MOD_PIKK_1	300	306	PF00454	0.567
MOD_PK_1	228	234	PF00069	0.524
MOD_PKA_2	610	616	PF00069	0.533
MOD_Plk_1	214	220	PF00069	0.533
MOD_Plk_1	336	342	PF00069	0.637
MOD_Plk_1	508	514	PF00069	0.322
MOD_Plk_2-3	187	193	PF00069	0.525
MOD_Plk_2-3	214	220	PF00069	0.558
MOD_Plk_4	175	181	PF00069	0.603
MOD_Plk_4	214	220	PF00069	0.533
MOD_Plk_4	228	234	PF00069	0.648
MOD_Plk_4	290	296	PF00069	0.556
MOD_Plk_4	557	563	PF00069	0.507
MOD_Plk_4	61	67	PF00069	0.522
MOD_Plk_4	612	618	PF00069	0.533
MOD_ProDKin_1	244	250	PF00069	0.637
MOD_ProDKin_1	253	259	PF00069	0.643
MOD_ProDKin_1	263	269	PF00069	0.617
MOD_ProDKin_1	275	281	PF00069	0.609
MOD_ProDKin_1	285	291	PF00069	0.727
MOD_ProDKin_1	374	380	PF00069	0.689
MOD_ProDKin_1	404	410	PF00069	0.683
MOD_ProDKin_1	440	446	PF00069	0.627
MOD_ProDKin_1	459	465	PF00069	0.605
MOD_ProDKin_1	468	474	PF00069	0.652
MOD_ProDKin_1	497	503	PF00069	0.735
MOD_SUMO_for_1	174	177	PF00179	0.522
MOD_SUMO_for_1	48	51	PF00179	0.533
MOD_SUMO_rev_2	220	229	PF00179	0.669
MOD_SUMO_rev_2	236	245	PF00179	0.667
MOD_SUMO_rev_2	45	55	PF00179	0.533
MOD_SUMO_rev_2	629	638	PF00179	0.493
MOD_SUMO_rev_2	95	100	PF00179	0.533
TRG_DiLeu_BaEn_1	10	15	PF01217	0.493
TRG_DiLeu_LyEn_5	10	15	PF01217	0.493
TRG_ENDOCYTIC_2	219	222	PF00928	0.493
TRG_ENDOCYTIC_2	25	28	PF00928	0.493
TRG_ENDOCYTIC_2	553	556	PF00928	0.536
TRG_ENDOCYTIC_2	596	599	PF00928	0.533
TRG_ENDOCYTIC_2	614	617	PF00928	0.533
TRG_ENDOCYTIC_2	635	638	PF00928	0.493
TRG_ER_diArg_1	149	152	PF00400	0.533
TRG_ER_diArg_1	523	526	PF00400	0.533
TRG_NES_CRM1_1	198	214	PF08389	0.612

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMJ8	Leptomonas seymouri	65%	95%
A0A3S5H7F0	Leishmania donovani	87%	100%
A4I1N2	Leishmania infantum	87%	100%
C9ZK81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9AXR6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	99%
Q4Q9Q5	Leishmania major	87%	99%