NPL4 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NPL4 family, putative

Species:

Leishmania braziliensis

UniProt:

A4HE71_LEIBR

TriTrypDb:

LbrM.25.1250 , LBRM2903_250022100

Length:

427

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HE71

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HE71

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006511	ubiquitin-dependent protein catabolic process	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009056	catabolic process	2	11
GO:0009057	macromolecule catabolic process	4	11
GO:0009987	cellular process	1	11
GO:0019538	protein metabolic process	3	11
GO:0019941	modification-dependent protein catabolic process	6	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043632	modification-dependent macromolecule catabolic process	5	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044248	cellular catabolic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044265	obsolete cellular macromolecule catabolic process	4	11
GO:0051603	proteolysis involved in protein catabolic process	5	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901575	organic substance catabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031625	ubiquitin protein ligase binding	5	1
GO:0032182	ubiquitin-like protein binding	3	1
GO:0043130	ubiquitin binding	4	1
GO:0044389	ubiquitin-like protein ligase binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	2	6	PF00656	0.499
CLV_NRD_NRD_1	16	18	PF00675	0.447
CLV_NRD_NRD_1	171	173	PF00675	0.227
CLV_NRD_NRD_1	30	32	PF00675	0.492
CLV_NRD_NRD_1	310	312	PF00675	0.408
CLV_NRD_NRD_1	368	370	PF00675	0.437
CLV_PCSK_KEX2_1	15	17	PF00082	0.459
CLV_PCSK_KEX2_1	171	173	PF00082	0.244
CLV_PCSK_KEX2_1	310	312	PF00082	0.408
CLV_PCSK_KEX2_1	368	370	PF00082	0.400
CLV_PCSK_KEX2_1	58	60	PF00082	0.440
CLV_PCSK_PC1ET2_1	368	370	PF00082	0.400
CLV_PCSK_PC1ET2_1	58	60	PF00082	0.440
CLV_PCSK_PC7_1	11	17	PF00082	0.555
CLV_PCSK_SKI1_1	163	167	PF00082	0.351
CLV_PCSK_SKI1_1	192	196	PF00082	0.348
CLV_PCSK_SKI1_1	311	315	PF00082	0.466
CLV_PCSK_SKI1_1	324	328	PF00082	0.273
CLV_PCSK_SKI1_1	341	345	PF00082	0.379
CLV_PCSK_SKI1_1	408	412	PF00082	0.452
DEG_APCC_DBOX_1	3	11	PF00400	0.353
DEG_APCC_DBOX_1	90	98	PF00400	0.465
DEG_SPOP_SBC_1	70	74	PF00917	0.464
DOC_MAPK_gen_1	160	170	PF00069	0.530
DOC_MAPK_gen_1	368	374	PF00069	0.439
DOC_PP1_RVXF_1	82	88	PF00149	0.407
DOC_PP4_FxxP_1	335	338	PF00568	0.389
DOC_PP4_MxPP_1	352	355	PF00568	0.418
DOC_USP7_MATH_1	217	221	PF00917	0.639
DOC_USP7_MATH_1	239	243	PF00917	0.529
DOC_USP7_MATH_1	400	404	PF00917	0.432
DOC_WW_Pin1_4	24	29	PF00397	0.658
DOC_WW_Pin1_4	257	262	PF00397	0.331
DOC_WW_Pin1_4	313	318	PF00397	0.345
DOC_WW_Pin1_4	334	339	PF00397	0.361
LIG_14-3-3_CanoR_1	324	330	PF00244	0.314
LIG_14-3-3_CanoR_1	399	405	PF00244	0.333
LIG_Actin_WH2_2	157	173	PF00022	0.512
LIG_BIR_II_1	1	5	PF00653	0.518
LIG_BRCT_BRCA1_1	48	52	PF00533	0.397
LIG_Clathr_ClatBox_1	94	98	PF01394	0.395
LIG_FHA_1	128	134	PF00498	0.457
LIG_FHA_1	247	253	PF00498	0.577
LIG_FHA_1	25	31	PF00498	0.632
LIG_FHA_1	292	298	PF00498	0.518
LIG_FHA_1	325	331	PF00498	0.313
LIG_FHA_1	65	71	PF00498	0.559
LIG_FHA_1	90	96	PF00498	0.345
LIG_FHA_2	24	30	PF00498	0.519
LIG_FHA_2	300	306	PF00498	0.565
LIG_FHA_2	317	323	PF00498	0.441
LIG_FHA_2	391	397	PF00498	0.410
LIG_LIR_Gen_1	327	336	PF02991	0.323
LIG_LIR_Gen_1	380	388	PF02991	0.275
LIG_LIR_Gen_1	49	60	PF02991	0.476
LIG_LIR_Nem_3	147	153	PF02991	0.490
LIG_LIR_Nem_3	323	329	PF02991	0.316
LIG_LIR_Nem_3	380	384	PF02991	0.273
LIG_LIR_Nem_3	49	55	PF02991	0.446
LIG_NRBOX	383	389	PF00104	0.358
LIG_Pex14_1	325	329	PF04695	0.312
LIG_Rb_pABgroove_1	120	128	PF01858	0.474
LIG_SH2_CRK	119	123	PF00017	0.438
LIG_SH2_CRK	417	421	PF00017	0.409
LIG_SH2_GRB2like	363	366	PF00017	0.416
LIG_SH2_NCK_1	126	130	PF00017	0.494
LIG_SH2_PTP2	329	332	PF00017	0.321
LIG_SH2_SRC	126	129	PF00017	0.530
LIG_SH2_STAP1	119	123	PF00017	0.480
LIG_SH2_STAP1	41	45	PF00017	0.521
LIG_SH2_STAT5	329	332	PF00017	0.321
LIG_SH2_STAT5	386	389	PF00017	0.450
LIG_SH3_3	135	141	PF00018	0.469
LIG_SH3_3	347	353	PF00018	0.481
LIG_SUMO_SIM_anti_2	5	13	PF11976	0.339
LIG_SUMO_SIM_par_1	247	253	PF11976	0.483
LIG_SUMO_SIM_par_1	5	13	PF11976	0.339
LIG_TYR_ITIM	189	194	PF00017	0.232
LIG_TYR_ITSM	325	332	PF00017	0.311
LIG_UBA3_1	51	58	PF00899	0.429
LIG_WRC_WIRS_1	105	110	PF05994	0.313
MOD_CDK_SPxxK_3	24	31	PF00069	0.507
MOD_CDK_SPxxK_3	334	341	PF00069	0.358
MOD_CK1_1	220	226	PF00069	0.621
MOD_CK1_1	242	248	PF00069	0.577
MOD_CK1_1	3	9	PF00069	0.418
MOD_CK1_1	316	322	PF00069	0.310
MOD_CK2_1	23	29	PF00069	0.381
MOD_CK2_1	391	397	PF00069	0.479
MOD_GlcNHglycan	222	226	PF01048	0.716
MOD_GlcNHglycan	227	230	PF01048	0.740
MOD_GlcNHglycan	236	239	PF01048	0.683
MOD_GlcNHglycan	241	244	PF01048	0.715
MOD_GlcNHglycan	305	309	PF01048	0.620
MOD_GlcNHglycan	41	44	PF01048	0.532
MOD_GlcNHglycan	48	51	PF01048	0.491
MOD_GSK3_1	195	202	PF00069	0.337
MOD_GSK3_1	217	224	PF00069	0.597
MOD_GSK3_1	242	249	PF00069	0.465
MOD_GSK3_1	311	318	PF00069	0.439
MOD_GSK3_1	320	327	PF00069	0.318
MOD_GSK3_1	386	393	PF00069	0.461
MOD_GSK3_1	400	407	PF00069	0.332
MOD_GSK3_1	61	68	PF00069	0.527
MOD_LATS_1	309	315	PF00433	0.487
MOD_N-GLC_1	225	230	PF02516	0.501
MOD_N-GLC_2	34	36	PF02516	0.505
MOD_NEK2_1	234	239	PF00069	0.500
MOD_NEK2_1	345	350	PF00069	0.416
MOD_PIKK_1	386	392	PF00454	0.460
MOD_PIKK_1	71	77	PF00454	0.433
MOD_PIKK_1	89	95	PF00454	0.372
MOD_PKA_2	245	251	PF00069	0.392
MOD_PKA_2	3	9	PF00069	0.416
MOD_PKA_2	345	351	PF00069	0.380
MOD_PKB_1	15	23	PF00069	0.421
MOD_Plk_1	199	205	PF00069	0.322
MOD_Plk_1	246	252	PF00069	0.464
MOD_Plk_1	80	86	PF00069	0.393
MOD_Plk_4	104	110	PF00069	0.325
MOD_Plk_4	246	252	PF00069	0.592
MOD_Plk_4	3	9	PF00069	0.356
MOD_Plk_4	325	331	PF00069	0.358
MOD_Plk_4	61	67	PF00069	0.443
MOD_ProDKin_1	24	30	PF00069	0.655
MOD_ProDKin_1	257	263	PF00069	0.328
MOD_ProDKin_1	313	319	PF00069	0.338
MOD_ProDKin_1	334	340	PF00069	0.356
MOD_SUMO_rev_2	107	116	PF00179	0.425
MOD_SUMO_rev_2	54	60	PF00179	0.389
TRG_DiLeu_BaLyEn_6	56	61	PF01217	0.446
TRG_ENDOCYTIC_2	119	122	PF00928	0.438
TRG_ENDOCYTIC_2	191	194	PF00928	0.350
TRG_ENDOCYTIC_2	329	332	PF00928	0.321
TRG_ENDOCYTIC_2	417	420	PF00928	0.385
TRG_ER_diArg_1	15	17	PF00400	0.448
TRG_ER_diArg_1	170	172	PF00400	0.427
TRG_ER_diArg_1	310	312	PF00400	0.408
TRG_ER_diArg_1	84	87	PF00400	0.280
TRG_NES_CRM1_1	376	390	PF08389	0.383
TRG_NLS_MonoExtN_4	367	372	PF00514	0.432

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I4H2	Leptomonas seymouri	69%	99%
A0A0S4KHU4	Bodo saltans	40%	100%
A0A1X0NM08	Trypanosomatidae	47%	100%
A0A3S7WZ51	Leishmania donovani	89%	100%
A0A422P221	Trypanosoma rangeli	49%	100%
C9ZK00	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	85%
E9AXK7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	68%
Q4Q9W4	Leishmania major	88%	100%
V5BCY4	Trypanosoma cruzi	49%	100%