Diphthine--ammonia ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MJ0570-related uncharacterized domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4HDX7_LEIBR

TriTrypDb:

LbrM.25.0300 , LBRM2903_250008300

Length:

614

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4HDX7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HDX7

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	1
GO:0006448	regulation of translational elongation	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0017182	peptidyl-diphthamide metabolic process	7	1
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018202	peptidyl-histidine modification	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0034248	regulation of amide metabolic process	5	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1900247	regulation of cytoplasmic translational elongation	8	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0016874	ligase activity	2	10
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	10
GO:0016880	acid-ammonia (or amide) ligase activity	4	10
GO:0017178	diphthine-ammonia ligase activity	5	10
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	327	329	PF00675	0.442
CLV_NRD_NRD_1	464	466	PF00675	0.379
CLV_NRD_NRD_1	81	83	PF00675	0.257
CLV_PCSK_KEX2_1	327	329	PF00082	0.346
CLV_PCSK_KEX2_1	464	466	PF00082	0.379
CLV_PCSK_KEX2_1	81	83	PF00082	0.249
CLV_PCSK_KEX2_1	86	88	PF00082	0.344
CLV_PCSK_PC1ET2_1	86	88	PF00082	0.224
CLV_PCSK_PC7_1	82	88	PF00082	0.365
CLV_PCSK_SKI1_1	112	116	PF00082	0.243
CLV_PCSK_SKI1_1	327	331	PF00082	0.456
CLV_PCSK_SKI1_1	516	520	PF00082	0.279
DEG_APCC_DBOX_1	146	154	PF00400	0.272
DEG_APCC_DBOX_1	198	206	PF00400	0.320
DEG_Nend_UBRbox_1	1	4	PF02207	0.249
DOC_CYCLIN_RxL_1	325	334	PF00134	0.327
DOC_CYCLIN_RxL_1	532	542	PF00134	0.407
DOC_CYCLIN_yCln2_LP_2	225	231	PF00134	0.374
DOC_MAPK_FxFP_2	285	288	PF00069	0.321
DOC_PP1_RVXF_1	138	144	PF00149	0.305
DOC_PP2B_LxvP_1	432	435	PF13499	0.338
DOC_PP2B_LxvP_1	76	79	PF13499	0.365
DOC_PP4_FxxP_1	285	288	PF00568	0.321
DOC_PP4_FxxP_1	385	388	PF00568	0.377
DOC_USP7_MATH_1	236	240	PF00917	0.171
DOC_USP7_MATH_1	286	290	PF00917	0.244
DOC_USP7_MATH_1	307	311	PF00917	0.615
DOC_USP7_MATH_1	354	358	PF00917	0.511
DOC_USP7_MATH_1	388	392	PF00917	0.426
DOC_USP7_MATH_1	402	406	PF00917	0.409
DOC_USP7_MATH_1	506	510	PF00917	0.267
DOC_WW_Pin1_4	248	253	PF00397	0.374
DOC_WW_Pin1_4	298	303	PF00397	0.367
DOC_WW_Pin1_4	376	381	PF00397	0.435
DOC_WW_Pin1_4	477	482	PF00397	0.408
LIG_14-3-3_CanoR_1	296	305	PF00244	0.472
LIG_14-3-3_CanoR_1	417	421	PF00244	0.343
LIG_14-3-3_CanoR_1	474	484	PF00244	0.461
LIG_14-3-3_CanoR_1	535	540	PF00244	0.408
LIG_14-3-3_CanoR_1	598	602	PF00244	0.381
LIG_Actin_WH2_2	65	83	PF00022	0.272
LIG_BRCT_BRCA1_1	472	476	PF00533	0.308
LIG_DLG_GKlike_1	535	543	PF00625	0.225
LIG_FHA_1	1	7	PF00498	0.264
LIG_FHA_1	178	184	PF00498	0.244
LIG_FHA_1	220	226	PF00498	0.249
LIG_FHA_1	245	251	PF00498	0.311
LIG_FHA_1	422	428	PF00498	0.599
LIG_FHA_1	434	440	PF00498	0.368
LIG_FHA_1	485	491	PF00498	0.312
LIG_FHA_1	508	514	PF00498	0.329
LIG_FHA_1	549	555	PF00498	0.256
LIG_FHA_1	572	578	PF00498	0.364
LIG_FHA_1	592	598	PF00498	0.380
LIG_FHA_2	192	198	PF00498	0.365
LIG_FHA_2	236	242	PF00498	0.248
LIG_FHA_2	365	371	PF00498	0.307
LIG_FHA_2	490	496	PF00498	0.380
LIG_LIR_Gen_1	152	161	PF02991	0.365
LIG_LIR_Gen_1	389	400	PF02991	0.284
LIG_LIR_Gen_1	534	544	PF02991	0.363
LIG_LIR_Gen_1	576	587	PF02991	0.248
LIG_LIR_Gen_1	58	69	PF02991	0.249
LIG_LIR_Nem_3	108	114	PF02991	0.255
LIG_LIR_Nem_3	119	124	PF02991	0.332
LIG_LIR_Nem_3	379	385	PF02991	0.338
LIG_LIR_Nem_3	389	395	PF02991	0.352
LIG_LIR_Nem_3	534	539	PF02991	0.425
LIG_LIR_Nem_3	542	547	PF02991	0.348
LIG_LIR_Nem_3	576	582	PF02991	0.342
LIG_LIR_Nem_3	58	64	PF02991	0.249
LIG_MYND_3	34	38	PF01753	0.171
LIG_Pex14_2	381	385	PF04695	0.355
LIG_Pex14_2	540	544	PF04695	0.492
LIG_SH2_CRK	111	115	PF00017	0.272
LIG_SH2_CRK	61	65	PF00017	0.365
LIG_SH2_NCK_1	420	424	PF00017	0.351
LIG_SH2_STAP1	220	224	PF00017	0.365
LIG_SH2_STAP1	400	404	PF00017	0.403
LIG_SH2_STAT3	231	234	PF00017	0.171
LIG_SH2_STAT3	59	62	PF00017	0.249
LIG_SH2_STAT5	155	158	PF00017	0.365
LIG_SH2_STAT5	284	287	PF00017	0.252
LIG_SH2_STAT5	536	539	PF00017	0.362
LIG_SH2_STAT5	84	87	PF00017	0.197
LIG_SH3_3	404	410	PF00018	0.295
LIG_SUMO_SIM_anti_2	238	244	PF11976	0.330
LIG_SUMO_SIM_anti_2	3	8	PF11976	0.278
LIG_SUMO_SIM_par_1	2	8	PF11976	0.249
LIG_SUMO_SIM_par_1	412	419	PF11976	0.298
LIG_SUMO_SIM_par_1	550	557	PF11976	0.284
LIG_TRAF2_1	101	104	PF00917	0.313
LIG_TRAF2_1	194	197	PF00917	0.308
LIG_TRAF2_1	266	269	PF00917	0.371
LIG_TRAF2_1	609	612	PF00917	0.617
LIG_UBA3_1	113	118	PF00899	0.273
MOD_CDK_SPK_2	298	303	PF00069	0.335
MOD_CDK_SPxK_1	477	483	PF00069	0.358
MOD_CK1_1	273	279	PF00069	0.315
MOD_CK1_1	310	316	PF00069	0.635
MOD_CK1_1	418	424	PF00069	0.449
MOD_CK2_1	191	197	PF00069	0.350
MOD_CK2_1	235	241	PF00069	0.248
MOD_CK2_1	263	269	PF00069	0.316
MOD_CK2_1	310	316	PF00069	0.565
MOD_CK2_1	354	360	PF00069	0.405
MOD_CK2_1	364	370	PF00069	0.392
MOD_CK2_1	383	389	PF00069	0.368
MOD_CK2_1	589	595	PF00069	0.434
MOD_GlcNHglycan	128	131	PF01048	0.308
MOD_GlcNHglycan	252	255	PF01048	0.344
MOD_GlcNHglycan	265	268	PF01048	0.391
MOD_GlcNHglycan	298	301	PF01048	0.502
MOD_GlcNHglycan	313	316	PF01048	0.709
MOD_GlcNHglycan	356	359	PF01048	0.483
MOD_GlcNHglycan	477	480	PF01048	0.301
MOD_GlcNHglycan	591	594	PF01048	0.402
MOD_GlcNHglycan	7	10	PF01048	0.269
MOD_GSK3_1	244	251	PF00069	0.305
MOD_GSK3_1	294	301	PF00069	0.521
MOD_GSK3_1	307	314	PF00069	0.658
MOD_GSK3_1	318	325	PF00069	0.607
MOD_GSK3_1	36	43	PF00069	0.252
MOD_GSK3_1	364	371	PF00069	0.441
MOD_GSK3_1	412	419	PF00069	0.475
MOD_GSK3_1	535	542	PF00069	0.369
MOD_GSK3_1	548	555	PF00069	0.255
MOD_GSK3_1	567	574	PF00069	0.409
MOD_GSK3_1	91	98	PF00069	0.389
MOD_N-GLC_1	305	310	PF02516	0.416
MOD_NEK2_1	177	182	PF00069	0.234
MOD_NEK2_1	208	213	PF00069	0.268
MOD_NEK2_1	294	299	PF00069	0.480
MOD_NEK2_1	305	310	PF00069	0.586
MOD_NEK2_1	331	336	PF00069	0.515
MOD_NEK2_1	412	417	PF00069	0.417
MOD_NEK2_1	475	480	PF00069	0.416
MOD_NEK2_1	507	512	PF00069	0.345
MOD_NEK2_1	539	544	PF00069	0.466
MOD_NEK2_1	60	65	PF00069	0.365
MOD_PIKK_1	458	464	PF00454	0.490
MOD_PIKK_1	507	513	PF00454	0.457
MOD_PIKK_1	568	574	PF00454	0.404
MOD_PK_1	179	185	PF00069	0.272
MOD_PKA_2	198	204	PF00069	0.365
MOD_PKA_2	416	422	PF00069	0.493
MOD_PKA_2	548	554	PF00069	0.358
MOD_PKA_2	597	603	PF00069	0.500
MOD_Plk_1	220	226	PF00069	0.337
MOD_Plk_1	514	520	PF00069	0.295
MOD_Plk_1	91	97	PF00069	0.365
MOD_Plk_2-3	489	495	PF00069	0.277
MOD_Plk_2-3	99	105	PF00069	0.171
MOD_Plk_4	10	16	PF00069	0.289
MOD_Plk_4	106	112	PF00069	0.232
MOD_Plk_4	220	226	PF00069	0.251
MOD_Plk_4	535	541	PF00069	0.311
MOD_Plk_4	548	554	PF00069	0.322
MOD_Plk_4	55	61	PF00069	0.365
MOD_Plk_4	91	97	PF00069	0.373
MOD_ProDKin_1	248	254	PF00069	0.365
MOD_ProDKin_1	298	304	PF00069	0.375
MOD_ProDKin_1	376	382	PF00069	0.432
MOD_ProDKin_1	477	483	PF00069	0.411
MOD_SUMO_for_1	117	120	PF00179	0.239
TRG_DiLeu_BaLyEn_6	109	114	PF01217	0.166
TRG_DiLeu_BaLyEn_6	290	295	PF01217	0.429
TRG_ENDOCYTIC_2	111	114	PF00928	0.272
TRG_ENDOCYTIC_2	155	158	PF00928	0.365
TRG_ENDOCYTIC_2	284	287	PF00928	0.252
TRG_ENDOCYTIC_2	382	385	PF00928	0.460
TRG_ENDOCYTIC_2	536	539	PF00928	0.362
TRG_ENDOCYTIC_2	61	64	PF00928	0.401
TRG_ER_diArg_1	138	141	PF00400	0.272
TRG_ER_diArg_1	326	328	PF00400	0.353
TRG_ER_diArg_1	464	466	PF00400	0.401
TRG_ER_diArg_1	80	82	PF00400	0.249
TRG_NES_CRM1_1	163	174	PF08389	0.331
TRG_NES_CRM1_1	595	608	PF08389	0.373
TRG_Pf-PMV_PEXEL_1	193	197	PF00026	0.365

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEV4	Leptomonas seymouri	59%	100%
A0A1X0P4I2	Trypanosomatidae	39%	100%
A0A3R7K7F7	Trypanosoma rangeli	39%	100%
A0A3S7WYW4	Leishmania donovani	79%	100%
A4I172	Leishmania infantum	79%	100%
D0A5F8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AXA6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
Q4QA66	Leishmania major	79%	100%
Q9USQ7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%