Cleavage and polyadenylation specificity factor subunit 2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor, putative

Species:

Leishmania braziliensis

UniProt:

A4HDW4_LEIBR

TriTrypDb:

LbrM.25.0170 , LBRM2903_250006900

Length:

829

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	11
GO:0005849	mRNA cleavage factor complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0140513	nuclear protein-containing complex	2	11
GO:0005654	nucleoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HDW4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HDW4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006378	mRNA polyadenylation	7	11
GO:0006379	mRNA cleavage	7	11
GO:0006396	RNA processing	6	11
GO:0006397	mRNA processing	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0016071	mRNA metabolic process	6	11
GO:0031123	RNA 3'-end processing	7	11
GO:0031124	mRNA 3'-end processing	8	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043631	RNA polyadenylation	6	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	11
GO:0090501	RNA phosphodiester bond hydrolysis	6	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006398	mRNA 3'-end processing by stem-loop binding and cleavage	8	1
GO:0008334	histone mRNA metabolic process	7	1
GO:0098787	mRNA cleavage involved in mRNA processing	8	1
GO:0098789	pre-mRNA cleavage required for polyadenylation	9	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0005488	binding	1	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	118	122	PF00656	0.563
CLV_C14_Caspase3-7	438	442	PF00656	0.539
CLV_NRD_NRD_1	392	394	PF00675	0.471
CLV_NRD_NRD_1	416	418	PF00675	0.515
CLV_NRD_NRD_1	589	591	PF00675	0.429
CLV_NRD_NRD_1	686	688	PF00675	0.355
CLV_NRD_NRD_1	723	725	PF00675	0.392
CLV_NRD_NRD_1	765	767	PF00675	0.457
CLV_NRD_NRD_1	796	798	PF00675	0.313
CLV_NRD_NRD_1	818	820	PF00675	0.335
CLV_PCSK_KEX2_1	307	309	PF00082	0.555
CLV_PCSK_KEX2_1	392	394	PF00082	0.448
CLV_PCSK_KEX2_1	415	417	PF00082	0.513
CLV_PCSK_KEX2_1	529	531	PF00082	0.682
CLV_PCSK_KEX2_1	549	551	PF00082	0.423
CLV_PCSK_KEX2_1	589	591	PF00082	0.493
CLV_PCSK_KEX2_1	686	688	PF00082	0.332
CLV_PCSK_KEX2_1	722	724	PF00082	0.335
CLV_PCSK_KEX2_1	765	767	PF00082	0.473
CLV_PCSK_KEX2_1	818	820	PF00082	0.424
CLV_PCSK_PC1ET2_1	307	309	PF00082	0.555
CLV_PCSK_PC1ET2_1	529	531	PF00082	0.675
CLV_PCSK_PC1ET2_1	549	551	PF00082	0.423
CLV_PCSK_SKI1_1	230	234	PF00082	0.442
CLV_PCSK_SKI1_1	276	280	PF00082	0.460
CLV_PCSK_SKI1_1	307	311	PF00082	0.475
CLV_PCSK_SKI1_1	34	38	PF00082	0.273
CLV_PCSK_SKI1_1	492	496	PF00082	0.777
CLV_PCSK_SKI1_1	592	596	PF00082	0.476
CLV_PCSK_SKI1_1	608	612	PF00082	0.224
CLV_PCSK_SKI1_1	642	646	PF00082	0.402
DEG_APCC_DBOX_1	591	599	PF00400	0.486
DEG_Nend_Nbox_1	1	3	PF02207	0.520
DOC_ANK_TNKS_1	796	803	PF00023	0.424
DOC_CKS1_1	809	814	PF01111	0.424
DOC_CYCLIN_RxL_1	224	235	PF00134	0.551
DOC_CYCLIN_RxL_1	506	518	PF00134	0.641
DOC_MAPK_gen_1	579	587	PF00069	0.547
DOC_MAPK_gen_1	589	595	PF00069	0.458
DOC_MAPK_gen_1	783	790	PF00069	0.424
DOC_MAPK_HePTP_8	252	264	PF00069	0.450
DOC_MAPK_MEF2A_6	241	250	PF00069	0.368
DOC_MAPK_MEF2A_6	255	264	PF00069	0.301
DOC_MAPK_MEF2A_6	492	501	PF00069	0.738
DOC_MAPK_MEF2A_6	579	587	PF00069	0.524
DOC_MAPK_MEF2A_6	783	790	PF00069	0.332
DOC_MAPK_RevD_3	785	798	PF00069	0.424
DOC_PP1_RVXF_1	32	38	PF00149	0.539
DOC_PP1_RVXF_1	383	389	PF00149	0.322
DOC_PP1_RVXF_1	508	515	PF00149	0.605
DOC_PP1_SILK_1	608	613	PF00149	0.408
DOC_PP2B_LxvP_1	499	502	PF13499	0.589
DOC_PP2B_PxIxI_1	327	333	PF00149	0.435
DOC_PP4_FxxP_1	182	185	PF00568	0.473
DOC_PP4_FxxP_1	198	201	PF00568	0.391
DOC_USP7_MATH_1	194	198	PF00917	0.453
DOC_USP7_MATH_1	456	460	PF00917	0.672
DOC_USP7_MATH_1	640	644	PF00917	0.514
DOC_USP7_MATH_1	737	741	PF00917	0.369
DOC_USP7_UBL2_3	146	150	PF12436	0.539
DOC_USP7_UBL2_3	230	234	PF12436	0.548
DOC_USP7_UBL2_3	608	612	PF12436	0.499
DOC_WW_Pin1_4	16	21	PF00397	0.515
DOC_WW_Pin1_4	493	498	PF00397	0.519
DOC_WW_Pin1_4	64	69	PF00397	0.473
DOC_WW_Pin1_4	808	813	PF00397	0.323
LIG_14-3-3_CanoR_1	3	11	PF00244	0.383
LIG_14-3-3_CanoR_1	554	561	PF00244	0.629
LIG_14-3-3_CanoR_1	592	602	PF00244	0.399
LIG_14-3-3_CanoR_1	722	731	PF00244	0.457
LIG_14-3-3_CanoR_1	818	822	PF00244	0.355
LIG_Actin_WH2_2	225	243	PF00022	0.415
LIG_Actin_WH2_2	670	688	PF00022	0.457
LIG_Actin_WH2_2	755	770	PF00022	0.341
LIG_Actin_WH2_2	802	820	PF00022	0.332
LIG_APCC_ABBA_1	787	792	PF00400	0.424
LIG_BIR_III_2	558	562	PF00653	0.725
LIG_BIR_III_4	483	487	PF00653	0.561
LIG_BRCT_BRCA1_1	113	117	PF00533	0.511
LIG_BRCT_BRCA1_1	210	214	PF00533	0.475
LIG_BRCT_BRCA1_1	336	340	PF00533	0.479
LIG_BRCT_BRCA1_1	647	651	PF00533	0.562
LIG_BRCT_BRCA1_1	753	757	PF00533	0.424
LIG_Clathr_ClatBox_1	27	31	PF01394	0.473
LIG_Clathr_ClatBox_1	787	791	PF01394	0.387
LIG_CtBP_PxDLS_1	659	663	PF00389	0.529
LIG_deltaCOP1_diTrp_1	38	45	PF00928	0.473
LIG_eIF4E_1	176	182	PF01652	0.473
LIG_FHA_1	114	120	PF00498	0.470
LIG_FHA_1	124	130	PF00498	0.473
LIG_FHA_1	201	207	PF00498	0.368
LIG_FHA_1	293	299	PF00498	0.404
LIG_FHA_1	45	51	PF00498	0.539
LIG_FHA_1	494	500	PF00498	0.632
LIG_FHA_1	694	700	PF00498	0.405
LIG_FHA_1	801	807	PF00498	0.424
LIG_FHA_1	809	815	PF00498	0.424
LIG_FHA_1	93	99	PF00498	0.495
LIG_FHA_2	116	122	PF00498	0.562
LIG_FHA_2	221	227	PF00498	0.565
LIG_FHA_2	291	297	PF00498	0.412
LIG_FHA_2	436	442	PF00498	0.537
LIG_GBD_Chelix_1	677	685	PF00786	0.424
LIG_KLC1_Yacidic_2	402	407	PF13176	0.596
LIG_LIR_Apic_2	106	112	PF02991	0.563
LIG_LIR_Apic_2	179	185	PF02991	0.473
LIG_LIR_Apic_2	197	201	PF02991	0.403
LIG_LIR_Apic_2	532	538	PF02991	0.540
LIG_LIR_Gen_1	130	139	PF02991	0.459
LIG_LIR_Gen_1	300	309	PF02991	0.563
LIG_LIR_Gen_1	402	412	PF02991	0.499
LIG_LIR_Gen_1	423	429	PF02991	0.694
LIG_LIR_Gen_1	775	786	PF02991	0.424
LIG_LIR_Nem_3	130	136	PF02991	0.459
LIG_LIR_Nem_3	231	236	PF02991	0.414
LIG_LIR_Nem_3	273	278	PF02991	0.401
LIG_LIR_Nem_3	300	304	PF02991	0.560
LIG_LIR_Nem_3	402	408	PF02991	0.499
LIG_LIR_Nem_3	423	428	PF02991	0.695
LIG_LIR_Nem_3	513	517	PF02991	0.683
LIG_LIR_Nem_3	775	781	PF02991	0.424
LIG_LIR_Nem_3	820	825	PF02991	0.333
LIG_NRBOX	262	268	PF00104	0.250
LIG_Pex14_1	172	176	PF04695	0.459
LIG_Pex14_1	41	45	PF04695	0.473
LIG_Pex14_2	37	41	PF04695	0.539
LIG_Pex14_2	45	49	PF04695	0.539
LIG_Rb_pABgroove_1	31	39	PF01858	0.539
LIG_RPA_C_Fungi	350	362	PF08784	0.533
LIG_SH2_CRK	109	113	PF00017	0.424
LIG_SH2_CRK	24	28	PF00017	0.430
LIG_SH2_CRK	275	279	PF00017	0.372
LIG_SH2_CRK	535	539	PF00017	0.538
LIG_SH2_CRK	825	829	PF00017	0.406
LIG_SH2_PTP2	405	408	PF00017	0.555
LIG_SH2_SRC	109	112	PF00017	0.424
LIG_SH2_STAP1	170	174	PF00017	0.457
LIG_SH2_STAP1	225	229	PF00017	0.423
LIG_SH2_STAP1	24	28	PF00017	0.437
LIG_SH2_STAP1	667	671	PF00017	0.495
LIG_SH2_STAP1	825	829	PF00017	0.625
LIG_SH2_STAT3	225	228	PF00017	0.433
LIG_SH2_STAT3	669	672	PF00017	0.482
LIG_SH2_STAT5	183	186	PF00017	0.312
LIG_SH2_STAT5	26	29	PF00017	0.385
LIG_SH2_STAT5	301	304	PF00017	0.556
LIG_SH2_STAT5	349	352	PF00017	0.432
LIG_SH2_STAT5	405	408	PF00017	0.555
LIG_SH2_STAT5	56	59	PF00017	0.260
LIG_SH2_STAT5	730	733	PF00017	0.326
LIG_SH3_3	17	23	PF00018	0.447
LIG_SH3_3	243	249	PF00018	0.351
LIG_SH3_3	364	370	PF00018	0.439
LIG_SH3_3	497	503	PF00018	0.730
LIG_SUMO_SIM_anti_2	359	366	PF11976	0.468
LIG_SUMO_SIM_par_1	205	211	PF11976	0.234
LIG_SUMO_SIM_par_1	26	31	PF11976	0.312
LIG_SUMO_SIM_par_1	359	366	PF11976	0.471
LIG_SUMO_SIM_par_1	658	664	PF11976	0.539
LIG_SUMO_SIM_par_1	785	792	PF11976	0.385
LIG_SUMO_SIM_par_1	801	808	PF11976	0.385
LIG_TRAF2_1	318	321	PF00917	0.410
LIG_TRAF2_1	397	400	PF00917	0.579
LIG_TRAF2_1	540	543	PF00917	0.657
LIG_TRAF2_1	771	774	PF00917	0.467
LIG_WRC_WIRS_1	104	109	PF05994	0.457
LIG_WRC_WIRS_1	133	138	PF05994	0.385
LIG_WRC_WIRS_1	195	200	PF05994	0.274
LIG_WW_2	367	370	PF00397	0.562
MOD_CK1_1	135	141	PF00069	0.457
MOD_CK1_1	219	225	PF00069	0.473
MOD_CK1_1	394	400	PF00069	0.589
MOD_CK1_1	468	474	PF00069	0.529
MOD_CK1_1	5	11	PF00069	0.359
MOD_CK1_1	521	527	PF00069	0.617
MOD_CK1_1	578	584	PF00069	0.533
MOD_CK1_1	596	602	PF00069	0.477
MOD_CK1_1	652	658	PF00069	0.570
MOD_CK1_1	693	699	PF00069	0.375
MOD_CK1_1	808	814	PF00069	0.341
MOD_CK2_1	10	16	PF00069	0.491
MOD_CK2_1	135	141	PF00069	0.424
MOD_CK2_1	220	226	PF00069	0.583
MOD_CK2_1	290	296	PF00069	0.417
MOD_CK2_1	315	321	PF00069	0.387
MOD_CK2_1	394	400	PF00069	0.565
MOD_CK2_1	537	543	PF00069	0.588
MOD_CK2_1	692	698	PF00069	0.405
MOD_CK2_1	768	774	PF00069	0.467
MOD_GlcNHglycan	317	320	PF01048	0.508
MOD_GlcNHglycan	342	345	PF01048	0.379
MOD_GlcNHglycan	396	399	PF01048	0.602
MOD_GlcNHglycan	4	7	PF01048	0.384
MOD_GlcNHglycan	470	473	PF01048	0.613
MOD_GlcNHglycan	523	526	PF01048	0.544
MOD_GlcNHglycan	597	601	PF01048	0.410
MOD_GlcNHglycan	655	658	PF01048	0.454
MOD_GlcNHglycan	705	709	PF01048	0.456
MOD_GlcNHglycan	753	756	PF01048	0.424
MOD_GlcNHglycan	774	777	PF01048	0.382
MOD_GSK3_1	111	118	PF00069	0.259
MOD_GSK3_1	128	135	PF00069	0.127
MOD_GSK3_1	185	192	PF00069	0.312
MOD_GSK3_1	216	223	PF00069	0.541
MOD_GSK3_1	466	473	PF00069	0.667
MOD_GSK3_1	488	495	PF00069	0.634
MOD_GSK3_1	5	12	PF00069	0.358
MOD_GSK3_1	506	513	PF00069	0.686
MOD_GSK3_1	606	613	PF00069	0.419
MOD_GSK3_1	64	71	PF00069	0.385
MOD_GSK3_1	645	652	PF00069	0.574
MOD_GSK3_1	661	668	PF00069	0.460
MOD_GSK3_1	673	680	PF00069	0.332
MOD_GSK3_1	688	695	PF00069	0.288
MOD_GSK3_1	722	729	PF00069	0.304
MOD_GSK3_1	733	740	PF00069	0.412
MOD_GSK3_1	768	775	PF00069	0.424
MOD_GSK3_1	800	807	PF00069	0.323
MOD_GSK3_1	99	106	PF00069	0.457
MOD_LATS_1	508	514	PF00433	0.729
MOD_N-GLC_1	219	224	PF02516	0.646
MOD_N-GLC_1	372	377	PF02516	0.309
MOD_NEK2_1	2	7	PF00069	0.433
MOD_NEK2_1	208	213	PF00069	0.405
MOD_NEK2_1	214	219	PF00069	0.460
MOD_NEK2_1	236	241	PF00069	0.426
MOD_NEK2_1	388	393	PF00069	0.443
MOD_NEK2_1	45	50	PF00069	0.385
MOD_NEK2_1	651	656	PF00069	0.569
MOD_NEK2_1	673	678	PF00069	0.350
MOD_NEK2_1	78	83	PF00069	0.467
MOD_NEK2_1	817	822	PF00069	0.374
MOD_NEK2_2	145	150	PF00069	0.334
MOD_NEK2_2	170	175	PF00069	0.363
MOD_NEK2_2	185	190	PF00069	0.269
MOD_NEK2_2	56	61	PF00069	0.204
MOD_NEK2_2	640	645	PF00069	0.312
MOD_NEK2_2	646	651	PF00069	0.278
MOD_PIKK_1	737	743	PF00454	0.420
MOD_PIKK_1	823	829	PF00454	0.513
MOD_PK_1	10	16	PF00069	0.251
MOD_PKA_1	722	728	PF00069	0.424
MOD_PKA_2	2	8	PF00069	0.417
MOD_PKA_2	220	226	PF00069	0.583
MOD_PKA_2	391	397	PF00069	0.509
MOD_PKA_2	688	694	PF00069	0.385
MOD_PKA_2	722	728	PF00069	0.424
MOD_PKA_2	817	823	PF00069	0.355
MOD_PKA_2	9	15	PF00069	0.345
MOD_PKB_1	766	774	PF00069	0.424
MOD_Plk_1	185	191	PF00069	0.312
MOD_Plk_1	372	378	PF00069	0.445
MOD_Plk_1	45	51	PF00069	0.361
MOD_Plk_1	640	646	PF00069	0.427
MOD_Plk_1	673	679	PF00069	0.424
MOD_Plk_2-3	334	340	PF00069	0.384
MOD_Plk_4	10	16	PF00069	0.388
MOD_Plk_4	128	134	PF00069	0.470
MOD_Plk_4	228	234	PF00069	0.484
MOD_Plk_4	372	378	PF00069	0.314
MOD_Plk_4	420	426	PF00069	0.441
MOD_Plk_4	45	51	PF00069	0.355
MOD_Plk_4	606	612	PF00069	0.465
MOD_Plk_4	646	652	PF00069	0.300
MOD_Plk_4	726	732	PF00069	0.271
MOD_Plk_4	753	759	PF00069	0.385
MOD_Plk_4	84	90	PF00069	0.424
MOD_Plk_4	99	105	PF00069	0.424
MOD_ProDKin_1	16	22	PF00069	0.507
MOD_ProDKin_1	493	499	PF00069	0.519
MOD_ProDKin_1	64	70	PF00069	0.332
MOD_ProDKin_1	808	814	PF00069	0.323
MOD_SUMO_for_1	149	152	PF00179	0.204
MOD_SUMO_rev_2	300	309	PF00179	0.544
TRG_DiLeu_BaEn_4	542	548	PF01217	0.675
TRG_DiLeu_BaLyEn_6	783	788	PF01217	0.424
TRG_ENDOCYTIC_2	133	136	PF00928	0.332
TRG_ENDOCYTIC_2	24	27	PF00928	0.438
TRG_ENDOCYTIC_2	275	278	PF00928	0.377
TRG_ENDOCYTIC_2	301	304	PF00928	0.556
TRG_ENDOCYTIC_2	405	408	PF00928	0.501
TRG_ENDOCYTIC_2	778	781	PF00928	0.424
TRG_ENDOCYTIC_2	825	828	PF00928	0.438
TRG_ER_diArg_1	392	395	PF00400	0.483
TRG_ER_diArg_1	415	417	PF00400	0.572
TRG_ER_diArg_1	589	592	PF00400	0.495
TRG_ER_diArg_1	685	687	PF00400	0.355
TRG_ER_diArg_1	722	724	PF00400	0.358
TRG_ER_diArg_1	764	766	PF00400	0.451
TRG_ER_diArg_1	817	819	PF00400	0.424
TRG_Pf-PMV_PEXEL_1	137	141	PF00026	0.409
TRG_Pf-PMV_PEXEL_1	34	38	PF00026	0.312
TRG_Pf-PMV_PEXEL_1	417	422	PF00026	0.587
TRG_Pf-PMV_PEXEL_1	765	769	PF00026	0.457

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEP3	Leptomonas seymouri	74%	100%
A0A0S4JJR9	Bodo saltans	30%	88%
A0A1X0P5N3	Trypanosomatidae	49%	100%
A0A3Q8IBL1	Leishmania donovani	89%	100%
A0A422NF58	Trypanosoma rangeli	50%	100%
A4I159	Leishmania infantum	89%	100%
D0A5E4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AX93	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QA79	Leishmania major	90%	100%
Q652P4	Oryza sativa subsp. japonica	23%	100%