Vps54_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function N-terminal domain (DUF2450)/Protein of unknown function C-terminus (DUF2451), putative

Species:

Leishmania braziliensis

UniProt:

A4HDS8_LEIBR

TriTrypDb:

LbrM.24.2250 , LBRM2903_240030100

Length:

1016

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	11
GO:0032991	protein-containing complex	1	11
GO:0110165	cellular anatomical entity	1	11
GO:1990745	EARP complex	2	11

Expansion

Sequence features

A4HDS8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HDS8

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0009987	cellular process	1	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0016482	cytosolic transport	4	11
GO:0032456	endocytic recycling	5	11
GO:0042147	retrograde transport, endosome to Golgi	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0051668	localization within membrane	3	11
GO:0098876	vesicle-mediated transport to the plasma membrane	4	11

Molecular functions
Term	Name	Level	Count
GO:0000149	SNARE binding	3	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	115	119	PF00656	0.386
CLV_C14_Caspase3-7	174	178	PF00656	0.479
CLV_C14_Caspase3-7	684	688	PF00656	0.399
CLV_NRD_NRD_1	194	196	PF00675	0.354
CLV_NRD_NRD_1	54	56	PF00675	0.583
CLV_PCSK_KEX2_1	194	196	PF00082	0.430
CLV_PCSK_KEX2_1	232	234	PF00082	0.408
CLV_PCSK_KEX2_1	253	255	PF00082	0.438
CLV_PCSK_KEX2_1	54	56	PF00082	0.561
CLV_PCSK_KEX2_1	912	914	PF00082	0.581
CLV_PCSK_PC1ET2_1	232	234	PF00082	0.408
CLV_PCSK_PC1ET2_1	253	255	PF00082	0.438
CLV_PCSK_PC1ET2_1	912	914	PF00082	0.581
CLV_PCSK_PC7_1	190	196	PF00082	0.232
CLV_PCSK_SKI1_1	100	104	PF00082	0.367
CLV_PCSK_SKI1_1	1001	1005	PF00082	0.461
CLV_PCSK_SKI1_1	17	21	PF00082	0.474
CLV_PCSK_SKI1_1	27	31	PF00082	0.585
CLV_PCSK_SKI1_1	285	289	PF00082	0.502
CLV_PCSK_SKI1_1	342	346	PF00082	0.513
CLV_PCSK_SKI1_1	479	483	PF00082	0.454
CLV_PCSK_SKI1_1	533	537	PF00082	0.418
CLV_PCSK_SKI1_1	54	58	PF00082	0.558
CLV_PCSK_SKI1_1	893	897	PF00082	0.374
CLV_PCSK_SKI1_1	950	954	PF00082	0.449
DEG_APCC_DBOX_1	232	240	PF00400	0.354
DEG_APCC_KENBOX_2	212	216	PF00400	0.447
DEG_MDM2_SWIB_1	580	587	PF02201	0.323
DEG_Nend_UBRbox_2	1	3	PF02207	0.588
DEG_SCF_FBW7_2	880	887	PF00400	0.474
DEG_SPOP_SBC_1	569	573	PF00917	0.640
DEG_SPOP_SBC_1	640	644	PF00917	0.522
DEG_SPOP_SBC_1	745	749	PF00917	0.574
DOC_ANK_TNKS_1	132	139	PF00023	0.479
DOC_CKS1_1	490	495	PF01111	0.551
DOC_CKS1_1	729	734	PF01111	0.633
DOC_CKS1_1	881	886	PF01111	0.469
DOC_MAPK_gen_1	232	239	PF00069	0.354
DOC_MAPK_gen_1	285	293	PF00069	0.524
DOC_MAPK_gen_1	475	483	PF00069	0.334
DOC_MAPK_gen_1	852	860	PF00069	0.557
DOC_MAPK_JIP1_4	989	995	PF00069	0.524
DOC_MAPK_MEF2A_6	286	295	PF00069	0.503
DOC_MAPK_MEF2A_6	446	454	PF00069	0.487
DOC_PP1_RVXF_1	891	897	PF00149	0.433
DOC_PP2B_LxvP_1	618	621	PF13499	0.525
DOC_PP4_FxxP_1	303	306	PF00568	0.570
DOC_PP4_FxxP_1	490	493	PF00568	0.454
DOC_PP4_FxxP_1	602	605	PF00568	0.541
DOC_USP7_MATH_1	164	168	PF00917	0.476
DOC_USP7_MATH_1	569	573	PF00917	0.640
DOC_USP7_MATH_1	621	625	PF00917	0.506
DOC_USP7_MATH_1	782	786	PF00917	0.372
DOC_USP7_MATH_1	840	844	PF00917	0.535
DOC_USP7_MATH_1	885	889	PF00917	0.489
DOC_USP7_UBL2_3	101	105	PF12436	0.345
DOC_USP7_UBL2_3	13	17	PF12436	0.672
DOC_USP7_UBL2_3	20	24	PF12436	0.516
DOC_USP7_UBL2_3	27	31	PF12436	0.554
DOC_USP7_UBL2_3	529	533	PF12436	0.571
DOC_USP7_UBL2_3	908	912	PF12436	0.488
DOC_WW_Pin1_4	399	404	PF00397	0.600
DOC_WW_Pin1_4	489	494	PF00397	0.562
DOC_WW_Pin1_4	510	515	PF00397	0.574
DOC_WW_Pin1_4	521	526	PF00397	0.454
DOC_WW_Pin1_4	728	733	PF00397	0.579
DOC_WW_Pin1_4	798	803	PF00397	0.508
DOC_WW_Pin1_4	880	885	PF00397	0.487
LIG_14-3-3_CanoR_1	195	201	PF00244	0.368
LIG_14-3-3_CanoR_1	815	820	PF00244	0.347
LIG_14-3-3_CanoR_1	969	975	PF00244	0.311
LIG_Actin_WH2_2	26	41	PF00022	0.642
LIG_Actin_WH2_2	837	854	PF00022	0.579
LIG_APCC_ABBA_1	391	396	PF00400	0.519
LIG_APCC_ABBA_1	789	794	PF00400	0.250
LIG_BRCT_BRCA1_1	598	602	PF00533	0.543
LIG_EH_1	557	561	PF12763	0.457
LIG_EH1_1	450	458	PF00400	0.364
LIG_eIF4E_1	975	981	PF01652	0.455
LIG_FHA_1	167	173	PF00498	0.418
LIG_FHA_1	217	223	PF00498	0.311
LIG_FHA_1	24	30	PF00498	0.555
LIG_FHA_1	286	292	PF00498	0.446
LIG_FHA_1	3	9	PF00498	0.671
LIG_FHA_1	429	435	PF00498	0.440
LIG_FHA_1	476	482	PF00498	0.456
LIG_FHA_1	550	556	PF00498	0.594
LIG_FHA_1	569	575	PF00498	0.505
LIG_FHA_1	604	610	PF00498	0.769
LIG_FHA_1	625	631	PF00498	0.590
LIG_FHA_1	881	887	PF00498	0.490
LIG_FHA_2	121	127	PF00498	0.377
LIG_FHA_2	180	186	PF00498	0.337
LIG_FHA_2	682	688	PF00498	0.392
LIG_FHA_2	729	735	PF00498	0.635
LIG_GBD_Chelix_1	426	434	PF00786	0.439
LIG_LIR_Apic_2	300	306	PF02991	0.582
LIG_LIR_Apic_2	599	605	PF02991	0.541
LIG_LIR_Gen_1	367	376	PF02991	0.482
LIG_LIR_Gen_1	382	391	PF02991	0.287
LIG_LIR_Gen_1	687	697	PF02991	0.449
LIG_LIR_Gen_1	905	914	PF02991	0.441
LIG_LIR_Nem_3	276	280	PF02991	0.398
LIG_LIR_Nem_3	367	371	PF02991	0.503
LIG_LIR_Nem_3	382	386	PF02991	0.291
LIG_LIR_Nem_3	402	407	PF02991	0.565
LIG_LIR_Nem_3	458	464	PF02991	0.520
LIG_LIR_Nem_3	577	583	PF02991	0.505
LIG_LIR_Nem_3	649	655	PF02991	0.283
LIG_LIR_Nem_3	687	692	PF02991	0.429
LIG_LIR_Nem_3	752	758	PF02991	0.618
LIG_LIR_Nem_3	905	909	PF02991	0.425
LIG_LIR_Nem_3	973	978	PF02991	0.418
LIG_NRBOX	994	1000	PF00104	0.442
LIG_PDZ_Class_2	1011	1016	PF00595	0.599
LIG_Pex14_2	580	584	PF04695	0.522
LIG_PTB_Apo_2	116	123	PF02174	0.232
LIG_PTB_Phospho_1	116	122	PF10480	0.232
LIG_REV1ctd_RIR_1	100	108	PF16727	0.378
LIG_REV1ctd_RIR_1	54	64	PF16727	0.709
LIG_SH2_CRK	387	391	PF00017	0.487
LIG_SH2_CRK	404	408	PF00017	0.321
LIG_SH2_CRK	906	910	PF00017	0.428
LIG_SH2_NCK_1	683	687	PF00017	0.388
LIG_SH2_SRC	385	388	PF00017	0.493
LIG_SH2_STAP1	387	391	PF00017	0.489
LIG_SH2_STAP1	793	797	PF00017	0.465
LIG_SH2_STAP1	976	980	PF00017	0.493
LIG_SH2_STAT3	538	541	PF00017	0.554
LIG_SH2_STAT3	863	866	PF00017	0.474
LIG_SH2_STAT5	122	125	PF00017	0.447
LIG_SH2_STAT5	268	271	PF00017	0.447
LIG_SH2_STAT5	308	311	PF00017	0.304
LIG_SH2_STAT5	368	371	PF00017	0.524
LIG_SH2_STAT5	385	388	PF00017	0.355
LIG_SH2_STAT5	538	541	PF00017	0.443
LIG_SH2_STAT5	681	684	PF00017	0.374
LIG_SH2_STAT5	730	733	PF00017	0.527
LIG_SH2_STAT5	863	866	PF00017	0.420
LIG_SH2_STAT5	947	950	PF00017	0.416
LIG_SH3_3	519	525	PF00018	0.402
LIG_SH3_3	631	637	PF00018	0.555
LIG_SH3_3	658	664	PF00018	0.503
LIG_SH3_3	799	805	PF00018	0.449
LIG_SH3_3	876	882	PF00018	0.533
LIG_SH3_3	939	945	PF00018	0.485
LIG_SUMO_SIM_anti_2	202	208	PF11976	0.442
LIG_SUMO_SIM_anti_2	288	295	PF11976	0.518
LIG_SUMO_SIM_anti_2	428	436	PF11976	0.424
LIG_SUMO_SIM_anti_2	785	791	PF11976	0.376
LIG_SUMO_SIM_anti_2	977	982	PF11976	0.491
LIG_SUMO_SIM_par_1	430	436	PF11976	0.470
LIG_SUMO_SIM_par_1	804	809	PF11976	0.388
LIG_TRAF2_1	84	87	PF00917	0.644
LIG_TYR_ITSM	971	978	PF00017	0.412
LIG_UBA3_1	222	227	PF00899	0.359
LIG_UBA3_1	236	244	PF00899	0.305
LIG_WRC_WIRS_1	528	533	PF05994	0.600
LIG_WRC_WIRS_1	686	691	PF05994	0.544
LIG_WRC_WIRS_1	926	931	PF05994	0.406
MOD_CDK_SPK_2	489	494	PF00069	0.562
MOD_CDK_SPK_2	728	733	PF00069	0.616
MOD_CK1_1	196	202	PF00069	0.232
MOD_CK1_1	327	333	PF00069	0.287
MOD_CK1_1	455	461	PF00069	0.475
MOD_CK1_1	510	516	PF00069	0.571
MOD_CK1_1	624	630	PF00069	0.566
MOD_CK1_1	639	645	PF00069	0.443
MOD_CK1_1	688	694	PF00069	0.551
MOD_CK1_1	749	755	PF00069	0.446
MOD_CK1_1	785	791	PF00069	0.376
MOD_CK1_1	907	913	PF00069	0.525
MOD_CK1_1	970	976	PF00069	0.290
MOD_CK2_1	120	126	PF00069	0.383
MOD_CK2_1	164	170	PF00069	0.378
MOD_CK2_1	340	346	PF00069	0.415
MOD_CK2_1	76	82	PF00069	0.650
MOD_CK2_1	851	857	PF00069	0.577
MOD_Cter_Amidation	230	233	PF01082	0.479
MOD_GlcNHglycan	166	169	PF01048	0.417
MOD_GlcNHglycan	241	244	PF01048	0.434
MOD_GlcNHglycan	41	44	PF01048	0.645
MOD_GlcNHglycan	509	512	PF01048	0.363
MOD_GlcNHglycan	618	621	PF01048	0.656
MOD_GlcNHglycan	643	646	PF01048	0.451
MOD_GlcNHglycan	768	771	PF01048	0.651
MOD_GSK3_1	120	127	PF00069	0.232
MOD_GSK3_1	146	153	PF00069	0.232
MOD_GSK3_1	175	182	PF00069	0.413
MOD_GSK3_1	281	288	PF00069	0.425
MOD_GSK3_1	428	435	PF00069	0.484
MOD_GSK3_1	570	577	PF00069	0.542
MOD_GSK3_1	626	633	PF00069	0.615
MOD_GSK3_1	636	643	PF00069	0.530
MOD_GSK3_1	681	688	PF00069	0.445
MOD_GSK3_1	744	751	PF00069	0.546
MOD_GSK3_1	76	83	PF00069	0.658
MOD_GSK3_1	761	768	PF00069	0.582
MOD_GSK3_1	970	977	PF00069	0.343
MOD_N-GLC_1	175	180	PF02516	0.438
MOD_N-GLC_1	777	782	PF02516	0.540
MOD_N-GLC_1	970	975	PF02516	0.413
MOD_NEK2_1	103	108	PF00069	0.448
MOD_NEK2_1	179	184	PF00069	0.335
MOD_NEK2_1	239	244	PF00069	0.388
MOD_NEK2_1	260	265	PF00069	0.442
MOD_NEK2_1	312	317	PF00069	0.304
MOD_NEK2_1	332	337	PF00069	0.341
MOD_NEK2_1	340	345	PF00069	0.403
MOD_NEK2_1	386	391	PF00069	0.380
MOD_NEK2_1	574	579	PF00069	0.504
MOD_NEK2_1	630	635	PF00069	0.535
MOD_NEK2_1	641	646	PF00069	0.434
MOD_NEK2_1	704	709	PF00069	0.434
MOD_NEK2_1	867	872	PF00069	0.519
MOD_NEK2_1	937	942	PF00069	0.555
MOD_NEK2_2	885	890	PF00069	0.500
MOD_OFUCOSY	320	325	PF10250	0.540
MOD_PIKK_1	199	205	PF00454	0.463
MOD_PIKK_1	231	237	PF00454	0.408
MOD_PIKK_1	312	318	PF00454	0.504
MOD_PIKK_1	559	565	PF00454	0.533
MOD_PIKK_1	574	580	PF00454	0.562
MOD_PIKK_1	621	627	PF00454	0.633
MOD_PIKK_1	806	812	PF00454	0.513
MOD_PK_1	25	31	PF00069	0.672
MOD_PK_1	254	260	PF00069	0.232
MOD_PK_1	438	444	PF00069	0.569
MOD_PKA_1	285	291	PF00069	0.547
MOD_PKA_2	193	199	PF00069	0.411
MOD_PKA_2	397	403	PF00069	0.579
MOD_PKA_2	761	767	PF00069	0.670
MOD_Plk_1	103	109	PF00069	0.343
MOD_Plk_1	175	181	PF00069	0.438
MOD_Plk_1	216	222	PF00069	0.324
MOD_Plk_1	260	266	PF00069	0.413
MOD_Plk_1	345	351	PF00069	0.435
MOD_Plk_1	505	511	PF00069	0.395
MOD_Plk_1	543	549	PF00069	0.514
MOD_Plk_1	569	575	PF00069	0.555
MOD_Plk_1	702	708	PF00069	0.433
MOD_Plk_1	777	783	PF00069	0.544
MOD_Plk_1	793	799	PF00069	0.357
MOD_Plk_1	856	862	PF00069	0.554
MOD_Plk_1	970	976	PF00069	0.389
MOD_Plk_2-3	76	82	PF00069	0.575
MOD_Plk_4	175	181	PF00069	0.440
MOD_Plk_4	25	31	PF00069	0.651
MOD_Plk_4	269	275	PF00069	0.415
MOD_Plk_4	327	333	PF00069	0.402
MOD_Plk_4	345	351	PF00069	0.452
MOD_Plk_4	386	392	PF00069	0.495
MOD_Plk_4	428	434	PF00069	0.511
MOD_Plk_4	452	458	PF00069	0.365
MOD_Plk_4	515	521	PF00069	0.530
MOD_Plk_4	527	533	PF00069	0.557
MOD_Plk_4	543	549	PF00069	0.625
MOD_Plk_4	570	576	PF00069	0.613
MOD_Plk_4	636	642	PF00069	0.460
MOD_Plk_4	681	687	PF00069	0.399
MOD_Plk_4	782	788	PF00069	0.392
MOD_Plk_4	859	865	PF00069	0.458
MOD_Plk_4	885	891	PF00069	0.461
MOD_Plk_4	932	938	PF00069	0.520
MOD_Plk_4	970	976	PF00069	0.329
MOD_Plk_4	979	985	PF00069	0.317
MOD_ProDKin_1	399	405	PF00069	0.593
MOD_ProDKin_1	489	495	PF00069	0.558
MOD_ProDKin_1	510	516	PF00069	0.576
MOD_ProDKin_1	521	527	PF00069	0.454
MOD_ProDKin_1	728	734	PF00069	0.590
MOD_ProDKin_1	798	804	PF00069	0.510
MOD_ProDKin_1	880	886	PF00069	0.477
MOD_SUMO_for_1	110	113	PF00179	0.261
MOD_SUMO_for_1	252	255	PF00179	0.440
MOD_SUMO_for_1	350	353	PF00179	0.455
MOD_SUMO_rev_2	134	140	PF00179	0.415
MOD_SUMO_rev_2	183	189	PF00179	0.447
MOD_SUMO_rev_2	96	103	PF00179	0.344
TRG_DiLeu_BaEn_2	246	252	PF01217	0.447
TRG_DiLeu_BaEn_2	496	502	PF01217	0.570
TRG_DiLeu_BaEn_4	675	681	PF01217	0.457
TRG_DiLeu_BaLyEn_6	799	804	PF01217	0.418
TRG_DiLeu_BaLyEn_6	890	895	PF01217	0.504
TRG_ENDOCYTIC_2	368	371	PF00928	0.524
TRG_ENDOCYTIC_2	387	390	PF00928	0.278
TRG_ENDOCYTIC_2	404	407	PF00928	0.330
TRG_ENDOCYTIC_2	683	686	PF00928	0.472
TRG_ENDOCYTIC_2	906	909	PF00928	0.425
TRG_ENDOCYTIC_2	975	978	PF00928	0.504
TRG_ER_diArg_1	193	195	PF00400	0.476
TRG_ER_diArg_1	53	55	PF00400	0.588
TRG_NES_CRM1_1	170	185	PF08389	0.479
TRG_NES_CRM1_1	428	443	PF08389	0.432
TRG_NLS_MonoExtC_3	910	916	PF00514	0.498
TRG_NLS_MonoExtN_4	908	915	PF00514	0.485
TRG_Pf-PMV_PEXEL_1	1001	1006	PF00026	0.512
TRG_Pf-PMV_PEXEL_1	141	145	PF00026	0.466
TRG_Pf-PMV_PEXEL_1	173	177	PF00026	0.378
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.401
TRG_Pf-PMV_PEXEL_1	424	428	PF00026	0.461
TRG_Pf-PMV_PEXEL_1	872	876	PF00026	0.567

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIU4	Leptomonas seymouri	72%	100%
A0A0S4KHU7	Bodo saltans	32%	100%
A0A1X0NVY5	Trypanosomatidae	45%	100%
A0A3S7WYM4	Leishmania donovani	84%	100%
A0A422NWJ9	Trypanosoma rangeli	44%	100%
A4I126	Leishmania infantum	84%	100%
E9AX63	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
Q4QAB3	Leishmania major	85%	100%
V5D5L5	Trypanosoma cruzi	45%	100%