Putative ubiquitin-conjugating enzyme e2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania braziliensis

UniProt:

A4HDN1_LEIBR

TriTrypDb:

LbrM.24.1770 , LBRM2903_240025400

Length:

192

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4HDN1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HDN1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0045116	protein neddylation	7	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0016874	ligase activity	2	8
GO:0016740	transferase activity	2	5
GO:0019787	ubiquitin-like protein transferase activity	3	4
GO:0019788	NEDD8 transferase activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	4
GO:0000166	nucleotide binding	3	3
GO:0005488	binding	1	3
GO:0005524	ATP binding	5	3
GO:0017076	purine nucleotide binding	4	3
GO:0030554	adenyl nucleotide binding	5	3
GO:0032553	ribonucleotide binding	3	3
GO:0032555	purine ribonucleotide binding	4	3
GO:0032559	adenyl ribonucleotide binding	5	3
GO:0035639	purine ribonucleoside triphosphate binding	4	3
GO:0036094	small molecule binding	2	3
GO:0043167	ion binding	2	3
GO:0043168	anion binding	3	3
GO:0097159	organic cyclic compound binding	2	3
GO:0097367	carbohydrate derivative binding	2	3
GO:1901265	nucleoside phosphate binding	3	3
GO:1901363	heterocyclic compound binding	2	3
GO:0019789	SUMO transferase activity	4	2
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016746	acyltransferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	108	110	PF00675	0.283
CLV_NRD_NRD_1	183	185	PF00675	0.201
CLV_NRD_NRD_1	9	11	PF00675	0.586
CLV_PCSK_KEX2_1	108	110	PF00082	0.376
CLV_PCSK_KEX2_1	183	185	PF00082	0.273
CLV_PCSK_SKI1_1	108	112	PF00082	0.190
CLV_PCSK_SKI1_1	11	15	PF00082	0.595
CLV_PCSK_SKI1_1	88	92	PF00082	0.363
DEG_SPOP_SBC_1	23	27	PF00917	0.539
DOC_MAPK_gen_1	121	128	PF00069	0.193
DOC_MAPK_MEF2A_6	121	130	PF00069	0.256
DOC_MAPK_MEF2A_6	141	149	PF00069	0.298
DOC_PP1_RVXF_1	86	92	PF00149	0.367
DOC_PP4_FxxP_1	151	154	PF00568	0.259
DOC_USP7_MATH_1	24	28	PF00917	0.628
DOC_USP7_MATH_1	33	37	PF00917	0.556
DOC_WW_Pin1_4	71	76	PF00397	0.412
LIG_14-3-3_CanoR_1	88	97	PF00244	0.354
LIG_BIR_II_1	1	5	PF00653	0.621
LIG_BRCT_BRCA1_1	91	95	PF00533	0.376
LIG_FHA_1	135	141	PF00498	0.267
LIG_FHA_2	137	143	PF00498	0.376
LIG_LIR_Apic_2	148	154	PF02991	0.259
LIG_LIR_Apic_2	94	100	PF02991	0.332
LIG_LIR_Gen_1	176	186	PF02991	0.376
LIG_LIR_Nem_3	176	181	PF02991	0.376
LIG_LIR_Nem_3	63	69	PF02991	0.440
LIG_LIR_Nem_3	72	76	PF02991	0.345
LIG_LIR_Nem_3	84	90	PF02991	0.332
LIG_PDZ_Class_2	187	192	PF00595	0.610
LIG_Pex14_2	87	91	PF04695	0.259
LIG_SH2_CRK	104	108	PF00017	0.341
LIG_SH2_CRK	73	77	PF00017	0.267
LIG_SH2_CRK	97	101	PF00017	0.406
LIG_SH2_SRC	156	159	PF00017	0.376
LIG_SH2_STAT5	104	107	PF00017	0.249
LIG_SH2_STAT5	67	70	PF00017	0.376
LIG_SH2_STAT5	73	76	PF00017	0.376
LIG_SUMO_SIM_par_1	142	148	PF11976	0.334
LIG_TYR_ITIM	71	76	PF00017	0.267
LIG_WRC_WIRS_1	35	40	PF05994	0.558
MOD_CDK_SPxK_1	71	77	PF00069	0.275
MOD_CK1_1	27	33	PF00069	0.637
MOD_CK1_1	34	40	PF00069	0.635
MOD_CK2_1	173	179	PF00069	0.364
MOD_Cter_Amidation	119	122	PF01082	0.175
MOD_GlcNHglycan	186	189	PF01048	0.488
MOD_GlcNHglycan	19	22	PF01048	0.551
MOD_GlcNHglycan	26	29	PF01048	0.584
MOD_GlcNHglycan	97	100	PF01048	0.285
MOD_GSK3_1	19	26	PF00069	0.637
MOD_GSK3_1	27	34	PF00069	0.694
MOD_N-GLC_1	28	33	PF02516	0.766
MOD_NEK2_1	19	24	PF00069	0.560
MOD_NEK2_2	136	141	PF00069	0.275
MOD_PKA_2	102	108	PF00069	0.283
MOD_PKA_2	134	140	PF00069	0.283
MOD_PKA_2	4	10	PF00069	0.522
MOD_ProDKin_1	71	77	PF00069	0.412
MOD_SUMO_for_1	171	174	PF00179	0.283
MOD_SUMO_rev_2	53	61	PF00179	0.412
TRG_DiLeu_BaEn_1	9	14	PF01217	0.475
TRG_DiLeu_BaLyEn_6	85	90	PF01217	0.175
TRG_ENDOCYTIC_2	73	76	PF00928	0.326
TRG_ER_diArg_1	107	109	PF00400	0.355
TRG_ER_diArg_1	183	185	PF00400	0.202
TRG_NES_CRM1_1	142	157	PF08389	0.376
TRG_NES_CRM1_1	50	65	PF08389	0.259
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.598
TRG_Pf-PMV_PEXEL_1	77	81	PF00026	0.312

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXA7	Leptomonas seymouri	27%	71%
A0A0N1IA93	Leptomonas seymouri	74%	100%
A0A0N1PB60	Leptomonas seymouri	28%	100%
A0A0S4JS17	Bodo saltans	27%	100%
A0A0S4KJ33	Bodo saltans	29%	100%
A0A1X0NT81	Trypanosomatidae	26%	92%
A0A1X0NVW4	Trypanosomatidae	47%	89%
A0A3Q8II46	Leishmania donovani	29%	100%
A0A3R7M279	Trypanosoma rangeli	44%	96%
A0A3S5H5C0	Leishmania donovani	28%	100%
A0A3S7WX16	Leishmania donovani	28%	74%
A0A3S7WYH9	Leishmania donovani	91%	100%
A0A422MVJ2	Trypanosoma rangeli	29%	100%
A0A422NDI3	Trypanosoma rangeli	26%	91%
A0A451EJH5	Leishmania donovani	33%	100%
A3KN22	Bos taurus	31%	100%
A4H3D5	Leishmania braziliensis	31%	100%
A4H402	Leishmania braziliensis	28%	100%
A4HCA6	Leishmania braziliensis	28%	74%
A4HMF2	Leishmania braziliensis	33%	100%
A4HRN6	Leishmania infantum	33%	100%
A4HS64	Leishmania infantum	28%	100%
A4HZT8	Leishmania infantum	28%	74%
A4IB24	Leishmania infantum	29%	100%
A7SE05	Nematostella vectensis	24%	94%
C9ZRM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	86%
C9ZWD4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	97%
E9AH78	Leishmania infantum	91%	100%
E9AK51	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AVP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	74%
E9AX16	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
E9B609	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
O13685	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
O74549	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P16577	Triticum aestivum	30%	100%
P42748	Arabidopsis thaliana	30%	100%
P42749	Arabidopsis thaliana	30%	100%
P42750	Arabidopsis thaliana	27%	100%
P52486	Drosophila melanogaster	28%	96%
P52491	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
P61081	Homo sapiens	31%	100%
P61082	Mus musculus	31%	100%
P61088	Homo sapiens	28%	100%
P61089	Mus musculus	28%	100%
P63146	Homo sapiens	27%	100%
P63147	Mus musculus	27%	100%
P63148	Oryctolagus cuniculus	27%	100%
P63149	Rattus norvegicus	27%	100%
Q08BH7	Danio rerio	29%	99%
Q0P5K3	Bos taurus	28%	100%
Q32LD2	Bos taurus	27%	98%
Q32P99	Bos taurus	27%	100%
Q4QAG0	Leishmania major	92%	100%
Q4QBT6	Leishmania major	28%	74%
Q4R4I1	Macaca fascicularis	28%	100%
Q54TI6	Dictyostelium discoideum	32%	83%
Q5R7J6	Pongo abelii	28%	100%
Q6C9W0	Yarrowia lipolytica (strain CLIB 122 / E 150)	28%	100%
Q6CSW8	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	30%	100%
Q6DCZ9	Xenopus laevis	32%	100%
Q6FVQ8	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q6P8D9	Xenopus tropicalis	32%	100%
Q75AF2	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	28%	100%
Q7ZY08	Xenopus laevis	31%	100%
Q9EQX9	Rattus norvegicus	28%	100%
Q9GNC1	Leishmania major	29%	97%
Q9LJD7	Arabidopsis thaliana	28%	100%
Q9NED4	Leishmania major	28%	100%
Q9NPD8	Homo sapiens	28%	97%
Q9SDY5	Arabidopsis thaliana	32%	100%
Q9UTN8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
Q9VSF3	Drosophila melanogaster	30%	100%
Q9W6H5	Danio rerio	33%	100%
Q9ZU75	Arabidopsis thaliana	30%	100%
V5BVS1	Trypanosoma cruzi	32%	86%